LOCUS       sequence-446063-       10823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct 
            with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in 
            vitro transcription detection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10823)
  AUTHORS   Walton RT, Qin Y, Blainey PC
  TITLE     CROPseq-multi: a versatile solution for multiplexed perturbation and
            decoding in pooled CRISPR screens
  JOURNAL   bioRxiv 2024.03.17.585235
REFERENCE   2  (bases 1 to 10823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.03.17.585235"
COMMENT     Sequence Label: CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW...
FEATURES             Location/Qualifiers
     source          1..10823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1489..1606
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1745..2923
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1976..2914
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2871..2891
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2930..2939
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2936..3646
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             3689..3709
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3728..4316
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3781..3801)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4199..4210)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4451..4469
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6497..6745)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(6555..6574)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(6725..6745)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     LTR             6792..6972
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7050..7171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7087..7106)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7141..7160
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7211..7346
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7367..7385)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7395..7412)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7395..7411)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7404..7426)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7553..8008
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7640..7659)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7850..7871
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8034..8138
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8139..8207,8208..8999)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8357..8376)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9170..9758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9659..9678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9912..9929
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10046..10067
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10082..10087,10088..10105,10106..10112)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10120..10136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10125..10147
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10144..10160
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10144..10160
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10179..10199
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10181..10199
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        10227..10606
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10608..10806
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10761..10781
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     1501 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     1561 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     1621 ggacagcaga gatccacttt ggcgccggct cgagggggcc cgggtgcaaa gatggataaa
     1681 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     1741 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     1801 ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga
     1861 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     1921 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     1981 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2041 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     2101 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     2161 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     2221 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     2281 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     2341 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     2401 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     2461 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     2521 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     2581 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     2641 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     2701 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     2761 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     2821 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     2881 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgacgtacgg ccaccatggt
     2941 gtctaagggc gaagagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
     3001 cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
     3061 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
     3121 ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga
     3181 cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgagga
     3241 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
     3301 cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact
     3361 cggctgggag gccttcaccg aaacgctgta ccccgctgac ggcggcctgg aaggcagaaa
     3421 cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacgcca agaccacata
     3481 tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag
     3541 actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt
     3601 ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaatagcg gtggcagcaa
     3661 gcgaaccgct gacggaagcg agtttgaacc caagaaaaag agaaaagttt aaacgcgtta
     3721 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     3781 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     3841 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     3901 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     3961 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     4021 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     4081 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     4141 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     4201 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     4261 gcgtgttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     4321 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     4381 gggggactgg aagggctaat tcactcccaa cgaagagaag atttcaccat tatcgtttca
     4441 gacccatttt taatacgact cactataggg aaaaaaagca ccgactcggt gccacttttt
     4501 caagttgata acggactagc cttatttcaa cttgctatgc tgttagagac gtacaaaaaa
     4561 gagcaagaag ctaaaaaaga tttaaaaatt atttttagcg cagttaatgg aacaggaact
     4621 aaatttaccc caaaaatatt acgtgaatca ggatataacg ttattgaggt tgaagagcat
     4681 gcatttgaag atgaaacatt taaaaatgtt gtaaatccaa atccagaatt tgatcctgca
     4741 tgaaaaatac cgcttgaata tggtattaaa catgatgcag atattattat tatgaatgac
     4801 ccagatgctg acagatttgg aatggcaata aaacatgatg gtcattttgt aagattagat
     4861 ggaaatcaaa caggaccaat tttaattgat tgaaaattat caaatctaaa acgcttaaat
     4921 agcattccaa aaaatccggc tctatattca agttttgtaa caagtgattt gggtgataga
     4981 atcgctcatg aaaaatatgg agttaatatt gtaaaaactt taactggatt taaatgaatg
     5041 ggtagagaaa ttgctaaaga agaagataac ggattaaatt ttgtttttgc ttatgaagaa
     5101 agttatggat atgtaattga tgactcagct agagataaag atggaataca agcttctata
     5161 ttaatagcag aggctgcttg attttataaa aaacaaaata aaacattagt agactattta
     5221 gaagatttat ttaaagaaat gggtgcatat tacactttca ctttaaactt gaattttaaa
     5281 ccagaagaaa agaaattaaa aattgaacca ttaatgaaat cattgagagc aacaccctta
     5341 actcaaattg ctggacttaa agttgttaat gttgaagact acatcgatgg aatgtataat
     5401 atgccaggac aagacttact aaaattttat ttagaagata agtcatgatt tgctgttcgc
     5461 ccaagtggaa ctgaacctaa actaaaaatt tattttatag gtgttggtga atctgttcaa
     5521 aacgctaaag ttaaagtaga cgaaattatt aaagaattaa aattaaaaat gaatatatag
     5581 gagaaaaaat gaaactaaac aaatatatag atcacacatt attaaaacaa gatgctacga
     5641 aagctgaaat taaacaatta tgtgatgaag caattgaatt tgattttgca acagtttgtg
     5701 ttaattcata ttgaacaagc tattgtaaag aattattaaa aggcacaaat gtaggaataa
     5761 caaatgttgt aggttttcct ctaggtgcat gcacaacagc tacaaaagca ttcgaagttt
     5821 ctgaagcaat taaagatggt gcaacagaaa ttgatatggt attaaatatt ggtgcattaa
     5881 aagacaaaaa ttatgaatta gttttagaag acatgaaagc tgtaaaaaaa gcagctggat
     5941 cacatgttgt taaatgtatt atggaaaatt gtttattaac aaaagaagaa atcatgaaag
     6001 cttgtgaaat agctgttgaa gctggattag aatttgttaa aacatcaaca ggattttcaa
     6061 aatcaggtgc aacatttgaa gatgttaaac taatgaagtc agttgttaaa gacaatgctt
     6121 tagttaaagc agctggtgga gttagaacat ttgaagatgc tcaaaaaatg attgaagcag
     6181 gagctgaccg cttaggaaca agtggtggag tagctattat taaaggtgaa gaaaacaacg
     6241 cgagttacta aaactagcgt ttttttattt tgctcatttt tattaaaagt ttgcaaaaag
     6301 gaacataaaa attctaatta ttgatactaa agttattaaa aagaagattt tggttgattt
     6361 tataaaggtc atagaatata atattttagc atgtgtattt tgtgtgctca tttacaaccg
     6421 tctctgcatt ggccgggaat cgaacccggg cctcccgcgt ggcaggcgag aattctacca
     6481 ctgaaccacc aatgccggtg tttcgtcctt tccacaagat atataaagcc aagaaatcga
     6541 aatactttca agttacggta agcatatgat agtccatttt aaaacataat tttaaaactg
     6601 caaactaccc aagaaattat tactttctac gtcacgtatt ttgtactaat atctttgtgt
     6661 ttacagtcaa attaattcta attatctctc taacagcctt gtatcgtata tgcaaatatg
     6721 aaggaatcat gggaaatagg ccctcaagct tggcgtaact agatcttgag acactgcttt
     6781 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6841 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6901 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6961 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     7021 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7081 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7141 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     7201 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7261 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7321 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     7381 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7441 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7501 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     7561 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7621 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7681 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7741 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7801 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7861 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7921 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7981 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     8041 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8101 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8161 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8221 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8281 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8341 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8401 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8461 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8521 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8581 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8641 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8701 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8761 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8821 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8881 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8941 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9001 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9061 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9121 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9181 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9241 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9301 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9361 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9421 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9481 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9541 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9601 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9661 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9721 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9781 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9841 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9901 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9961 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    10021 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    10081 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    10141 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
    10201 aacaaaagct ggagctgcaa gcttaagaca ttgattattg actagttatt aatagtaatc
    10261 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
    10321 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
    10381 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
    10441 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
    10501 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
    10561 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
    10621 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
    10681 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
    10741 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
    10801 aagcagcgcg ttttgcctgt act
//
