LOCUS       sequence-446065-       10820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct 
            with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7
            in vitro transcription detection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10820)
  AUTHORS   Walton RT, Qin Y, Blainey PC
  TITLE     CROPseq-multi: a versatile solution for multiplexed perturbation and
            decoding in pooled CRISPR screens
  JOURNAL   bioRxiv 2024.03.17.585235
REFERENCE   2  (bases 1 to 10820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.03.17.585235"
COMMENT     Sequence Label: CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entr...
FEATURES             Location/Qualifiers
     source          1..10820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1489..1606
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1745..2923
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1976..2914
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2871..2891
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2996..3706
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    3725..4313
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3778..3798)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4196..4207)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4448..4466
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6494..6742)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(6552..6571)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(6722..6742)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     LTR             6789..6969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7047..7168
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7084..7103)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7138..7157
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7208..7343
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7364..7382)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7392..7409)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7392..7408)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7401..7423)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7550..8005
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7637..7656)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7847..7868
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8031..8135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8136..8204,8205..8996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8354..8373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9167..9755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9656..9675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9909..9926
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10043..10064
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10079..10084,10085..10102,10103..10109)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10117..10133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10122..10144
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10141..10157
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10141..10157
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10176..10196
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10178..10196
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        10224..10603
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10605..10803
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10758..10778
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     1501 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     1561 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     1621 ggacagcaga gatccacttt ggcgccggct cgagggggcc cgggtgcaaa gatggataaa
     1681 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     1741 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     1801 ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga
     1861 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     1921 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     1981 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2041 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     2101 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     2161 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     2221 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     2281 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     2341 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     2401 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     2461 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     2521 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     2581 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     2641 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     2701 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     2761 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     2821 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     2881 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgacgtacgg ccaccatgct
     2941 gtgctgtatg agaagaacca aacaggttga aaagaatgat gaggaccaaa agatcatggt
     3001 gtctaagggc gaagagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
     3061 cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
     3121 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
     3181 ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga
     3241 cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgagga
     3301 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
     3361 cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact
     3421 cggctgggag gccttcaccg aaacgctgta ccccgctgac ggcggcctgg aaggcagaaa
     3481 cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacgcca agaccacata
     3541 tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag
     3601 actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt
     3661 ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaattaaa cgcgttaagt
     3721 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3781 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3841 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3901 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     3961 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4021 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4081 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     4141 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4201 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4261 tgttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     4321 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     4381 ggactggaag ggctaattca ctcccaacga agagaagatt tcaccattat cgtttcagac
     4441 ccatttttaa tacgactcac tatagggaaa aaaagcaccg actcggtgcc actttttcaa
     4501 gttgataacg gactagcctt atttcaactt gctatgctgt tagagacgta caaaaaagag
     4561 caagaagcta aaaaagattt aaaaattatt tttagcgcag ttaatggaac aggaactaaa
     4621 tttaccccaa aaatattacg tgaatcagga tataacgtta ttgaggttga agagcatgca
     4681 tttgaagatg aaacatttaa aaatgttgta aatccaaatc cagaatttga tcctgcatga
     4741 aaaataccgc ttgaatatgg tattaaacat gatgcagata ttattattat gaatgaccca
     4801 gatgctgaca gatttggaat ggcaataaaa catgatggtc attttgtaag attagatgga
     4861 aatcaaacag gaccaatttt aattgattga aaattatcaa atctaaaacg cttaaatagc
     4921 attccaaaaa atccggctct atattcaagt tttgtaacaa gtgatttggg tgatagaatc
     4981 gctcatgaaa aatatggagt taatattgta aaaactttaa ctggatttaa atgaatgggt
     5041 agagaaattg ctaaagaaga agataacgga ttaaattttg tttttgctta tgaagaaagt
     5101 tatggatatg taattgatga ctcagctaga gataaagatg gaatacaagc ttctatatta
     5161 atagcagagg ctgcttgatt ttataaaaaa caaaataaaa cattagtaga ctatttagaa
     5221 gatttattta aagaaatggg tgcatattac actttcactt taaacttgaa ttttaaacca
     5281 gaagaaaaga aattaaaaat tgaaccatta atgaaatcat tgagagcaac acccttaact
     5341 caaattgctg gacttaaagt tgttaatgtt gaagactaca tcgatggaat gtataatatg
     5401 ccaggacaag acttactaaa attttattta gaagataagt catgatttgc tgttcgccca
     5461 agtggaactg aacctaaact aaaaatttat tttataggtg ttggtgaatc tgttcaaaac
     5521 gctaaagtta aagtagacga aattattaaa gaattaaaat taaaaatgaa tatataggag
     5581 aaaaaatgaa actaaacaaa tatatagatc acacattatt aaaacaagat gctacgaaag
     5641 ctgaaattaa acaattatgt gatgaagcaa ttgaatttga ttttgcaaca gtttgtgtta
     5701 attcatattg aacaagctat tgtaaagaat tattaaaagg cacaaatgta ggaataacaa
     5761 atgttgtagg ttttcctcta ggtgcatgca caacagctac aaaagcattc gaagtttctg
     5821 aagcaattaa agatggtgca acagaaattg atatggtatt aaatattggt gcattaaaag
     5881 acaaaaatta tgaattagtt ttagaagaca tgaaagctgt aaaaaaagca gctggatcac
     5941 atgttgttaa atgtattatg gaaaattgtt tattaacaaa agaagaaatc atgaaagctt
     6001 gtgaaatagc tgttgaagct ggattagaat ttgttaaaac atcaacagga ttttcaaaat
     6061 caggtgcaac atttgaagat gttaaactaa tgaagtcagt tgttaaagac aatgctttag
     6121 ttaaagcagc tggtggagtt agaacatttg aagatgctca aaaaatgatt gaagcaggag
     6181 ctgaccgctt aggaacaagt ggtggagtag ctattattaa aggtgaagaa aacaacgcga
     6241 gttactaaaa ctagcgtttt tttattttgc tcatttttat taaaagtttg caaaaaggaa
     6301 cataaaaatt ctaattattg atactaaagt tattaaaaag aagattttgg ttgattttat
     6361 aaaggtcata gaatataata ttttagcatg tgtattttgt gtgctcattt acaaccgtct
     6421 ctgcattggc cgggaatcga acccgggcct cccgcgtggc aggcgagaat tctaccactg
     6481 aaccaccaat gccggtgttt cgtcctttcc acaagatata taaagccaag aaatcgaaat
     6541 actttcaagt tacggtaagc atatgatagt ccattttaaa acataatttt aaaactgcaa
     6601 actacccaag aaattattac tttctacgtc acgtattttg tactaatatc tttgtgttta
     6661 cagtcaaatt aattctaatt atctctctaa cagccttgta tcgtatatgc aaatatgaag
     6721 gaatcatggg aaataggccc tcaagcttgg cgtaactaga tcttgagaca ctgctttttg
     6781 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6961 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7021 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     7081 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7141 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     7201 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7261 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7321 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     7381 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     7441 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     7501 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     7561 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7621 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7681 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7741 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7801 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7861 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7921 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7981 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8041 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8101 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     8161 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     8221 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     8281 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     8341 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     8401 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     8461 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8521 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8581 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8641 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     8701 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8761 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8821 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8881 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8941 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     9001 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     9061 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9121 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     9181 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     9241 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     9301 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     9361 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     9421 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     9481 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     9541 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     9601 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     9661 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9721 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9781 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     9841 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     9901 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     9961 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    10021 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    10081 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    10141 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    10201 aaaagctgga gctgcaagct taagacattg attattgact agttattaat agtaatcaat
    10261 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
    10321 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
    10381 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
    10441 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
    10501 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
    10561 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
    10621 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
    10681 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
    10741 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
    10801 cagcgcgttt tgcctgtact
//
