LOCUS       sequence-446209-        8352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone (attL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   Contiliani DF, Sretenovic S, Dailey M, Zhou M, Cheng Y, Creste S, 
            Xiao S, Qi Y
  TITLE     Harnessing novel cytidine deaminases from the animal kingdom for 
            robust multiplexed base editing in rice.
  JOURNAL   Plant Biotechnol J. 2025 Feb 14. doi: 10.1111/pbi.70000.
  PUBMED    39950393
REFERENCE   2  (bases 1 to 8352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2025 Feb 14. doi: 10.1111/pbi.70000."
COMMENT     Sequence Label: pYPQ265_DlA3G
FEATURES             Location/Qualifiers
     source          1..8352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             358..1149
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1731..1750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1984..2001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2160..2187)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2279..2365)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2414..2436
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2428..2445
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2429..2445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2461..2560
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3212..3277
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3284..3304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3329..3352,3353..3355,3356..7429)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             7342..7350
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7430..7477
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7499..7747
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7760..7780
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7802..8054
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    8099..8222
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8239..8258)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8240..8258)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8263..8279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8263..8279)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcatgga
     2581 ggcgtccact gcgccctgga cacggtgtct gttggatgaa aacacgttca atgaaaactt
     2641 catgaaccaa atcaacccaa gaaaaactta tctttgctac aaggtcgaga tacttgatgg
     2701 cgacactagg gtgctgcttg acgagaagaa ggggtttgtc cagaacaaag gagcaaacga
     2761 acctggaaaa cccagacatg ccgaatcgta cttcttggac agaatcaggt cctggaatct
     2821 tgaccgggga cttcactatc gccttacctg ttttatctca tggacgccat gccatagctg
     2881 cgcgcaggaa cttgcaacgt ttttgggcga aaatagtcac ctgagcctcc atatctttgc
     2941 ctcgcggatt tactcgctgc cgggctacga agccgggctg aggaagttgc aggccgctgg
     3001 agcccagatt gcgataatga ctagcaaaga atttgagcac tgctgggaaa atttcgttga
     3061 ccatcaagat aggccattcc agccctggga cggtttggag gttgtttctc agcacctctg
     3121 caatagtctc caaactatac tgcagatgca ggagaattca ggttctgaga cccctggcac
     3181 aagtgagtca gcaacacccg agtccaccat ggattacaag gaccacgacg gggattacaa
     3241 ggaccacgac attgattaca aggatgatga tgacaagatg gctccgaaga agaagaggaa
     3301 ggttggcatc cacggggtgc cagctgctga caagaagtac tcgatcggcc tcgctattgg
     3361 gactaactct gttggctggg ccgtgatcac cgacgagtac aaggtgccct caaagaagtt
     3421 caaggtcctg ggcaacaccg atcggcattc catcaagaag aatctcattg gcgctctcct
     3481 gttcgacagc ggcgagacgg ctgaggctac gcggctcaag cgcaccgccc gcaggcggta
     3541 cacgcgcagg aagaatcgca tctgctacct gcaggagatt ttctccaacg agatggcgaa
     3601 ggttgacgat tctttcttcc acaggctgga ggagtcattc ctcgtggagg aggataagaa
     3661 gcacgagcgg catccaatct tcggcaacat tgtcgacgag gttgcctacc acgagaagta
     3721 ccctacgatc taccatctgc ggaagaagct cgtggactcc acagataagg cggacctccg
     3781 cctgatctac ctcgctctgg cccacatgat taagttcagg ggccatttcc tgatcgaggg
     3841 ggatctcaac ccggacaata gcgatgttga caagctgttc atccagctcg tgcagacgta
     3901 caaccagctc ttcgaggaga accccattaa tgcgtcaggc gtcgacgcga aggctatcct
     3961 gtccgctagg ctctcgaagt ctcggcgcct cgagaacctg atcgcccagc tgccgggcga
     4021 gaagaagaac ggcctgttcg ggaatctcat tgcgctcagc ctggggctca cgcccaactt
     4081 caagtcgaat ttcgatctcg ctgaggacgc caagctgcag ctctccaagg acacatacga
     4141 cgatgacctg gataacctcc tggcccagat cggcgatcag tacgcggacc tgttcctcgc
     4201 tgccaagaat ctgtcggacg ccatcctcct gtctgatatt ctcagggtga acaccgagat
     4261 tacgaaggct ccgctctcag cctccatgat caagcgctac gacgagcacc atcaggatct
     4321 gaccctcctg aaggcgctgg tcaggcagca gctccccgag aagtacaagg agatcttctt
     4381 cgatcagtcg aagaacggct acgctgggta cattgacggc ggggcctctc aggaggagtt
     4441 ctacaagttc atcaagccga ttctggagaa gatggacggc acggaggagc tgctggtgaa
     4501 gctcaatcgc gaggacctcc tgaggaagca gcggacattc gataacggca gcatcccaca
     4561 ccagattcat ctcggggagc tgcacgctat cctgaggagg caggaggact tctacccttt
     4621 cctcaaggat aaccgcgaga agatcgagaa gattctgact ttcaggatcc cgtactacgt
     4681 cggcccactc gctaggggca actcccgctt cgcttggatg acccgcaagt cagaggagac
     4741 gatcacgccg tggaacttcg aggaggtggt cgacaagggc gctagcgctc agtcgttcat
     4801 cgagaggatg acgaatttcg acaagaacct gccaaatgag aaggtgctcc ctaagcactc
     4861 gctcctgtac gagtacttca cagtctacaa cgagctgact aaggtgaagt atgtgaccga
     4921 gggcatgagg aagccggctt tcctgtctgg ggagcagaag aaggccatcg tggacctcct
     4981 gttcaagacc aaccggaagg tcacggttaa gcagctcaag gaggactact tcaagaagat
     5041 tgagtgcttc gattcggtcg agatctctgg cgttgaggac cgcttcaacg cctccctggg
     5101 gacctaccac gatctcctga agatcattaa ggataaggac ttcctggaca acgaggagaa
     5161 tgaggatatc ctcgaggaca ttgtgctgac actcactctg ttcgaggacc gggagatgat
     5221 cgaggagcgc ctgaagactt acgcccatct cttcgatgac aaggtcatga agcagctcaa
     5281 gaggaggagg tacaccggct gggggaggct gagcaggaag ctcatcaacg gcattcggga
     5341 caagcagtcc gggaagacga tcctcgactt cctgaagagc gatggcttcg cgaaccgcaa
     5401 tttcatgcag ctgattcacg atgacagcct cacattcaag gaggatatcc agaaggctca
     5461 ggtgagcggc cagggggact cgctgcacga gcatatcgcg aacctcgctg gctcgccagc
     5521 tatcaagaag gggattctgc agaccgtgaa ggttgtggac gagctggtga aggtcatggg
     5581 caggcacaag cctgagaaca tcgtcattga gatggcccgg gagaatcaga ccacgcagaa
     5641 gggccagaag aactcacgcg agaggatgaa gaggatcgag gagggcatta aggagctggg
     5701 gtcccagatc ctcaaggagc acccggtgga gaacacgcag ctgcagaatg agaagctcta
     5761 cctgtactac ctccagaatg gccgcgatat gtatgtggac caggagctgg atattaacag
     5821 gctcagcgat tacgacgtcg atcatatcgt tccacagtca ttcctgaagg atgactccat
     5881 tgacaacaag gtcctcacca ggtcggacaa gaaccggggc aagtctgata atgttccttc
     5941 agaggaggtc gttaagaaga tgaagaacta ctggcgccag ctcctgaatg ccaagctgat
     6001 cacgcagcgg aagttcgata acctcacaaa ggctgagagg ggcgggctct ctgagctgga
     6061 caaggcgggc ttcatcaaga ggcagctggt cgagacacgg cagatcacta agcacgttgc
     6121 gcagattctc gactcacgga tgaacactaa gtacgatgag aatgacaagc tgatccgcga
     6181 ggtgaaggtc atcaccctga agtcaaagct cgtctccgac ttcaggaagg atttccagtt
     6241 ctacaaggtt cgggagatca acaattacca ccatgcccat gacgcgtacc tgaacgcggt
     6301 ggtcggcaca gctctgatca agaagtaccc aaagctcgag agcgagttcg tgtacgggga
     6361 ctacaaggtt tacgatgtga ggaagatgat cgccaagtcg gagcaggaga ttggcaaggc
     6421 taccgccaag tacttcttct actctaacat tatgaatttc ttcaagacag agatcactct
     6481 ggccaatggc gagatccgga agcgccccct catcgagacg aacggcgaga cgggggagat
     6541 cgtgtgggac aagggcaggg atttcgcgac cgtcaggaag gttctctcca tgccacaagt
     6601 gaatatcgtc aagaagacag aggtccagac tggcgggttc tctaaggagt caattctgcc
     6661 taagcggaac agcgacaagc tcatcgcccg caagaaggac tgggatccga agaagtacgg
     6721 cgggttcgac agccccactg tggcctactc ggtcctggtt gtggcgaagg ttgagaaggg
     6781 caagtccaag aagctcaaga gcgtgaagga gctgctgggg atcacgatta tggagcgctc
     6841 cagcttcgag aagaacccga tcgatttcct ggaggcgaag ggctacaagg aggtgaagaa
     6901 ggacctgatc attaagctcc ccaagtactc actcttcgag ctggagaacg gcaggaagcg
     6961 gatgctggct tccgctggcg agctgcagaa ggggaacgag ctggctctgc cgtccaagta
     7021 tgtgaacttc ctctacctgg cctcccacta cgagaagctc aagggcagcc ccgaggacaa
     7081 cgagcagaag cagctgttcg tcgagcagca caagcattac ctcgacgaga tcattgagca
     7141 gatttccgag ttctccaagc gcgtgatcct ggccgacgcg aatctggata aggtcctctc
     7201 cgcgtacaac aagcaccgcg acaagccaat cagggagcag gctgagaata tcattcatct
     7261 cttcaccctg acgaacctcg gcgcccctgc tgctttcaag tacttcgaca caactatcga
     7321 tcgcaagagg tacacaagca ctaaggaggt cctggacgcg accctcatcc accagtcgat
     7381 taccggcctc tacgagacgc gcatcgacct gtctcagctc gggggcgaca agcggccagc
     7441 ggcgacgaag aaggcggggc aggcgaagaa gaagaaggga gacggatcag gcgggtcaac
     7501 aaacctcagt gacatcatag agaaggaaac tggtaagcaa ctggttattc aagagagcat
     7561 tctgatgctc cctgaggaag tcgaggaagt tataggaaac aagcctgaga gcgatatact
     7621 cgtgcatacg gcgtatgacg agagcacgga tgaaaatgtg atgctcttga ccagcgatgc
     7681 gccagaatat aaaccctggg cactggttat tcaggactct aacggggaaa ataaaatcaa
     7741 gatgctctca ggtggctccc caaagaagaa acgcaaggtt tagtggcggc cgccgacgtc
     7801 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     7861 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     7921 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     7981 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     8041 tatgttacta gatcgggaat tgatcccccc tcgacagctt ccggaaaggg cgaattcgca
     8101 actttgtata caaaagttga acgagaaacg taaaatgata taaatatcaa tatattaaat
     8161 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta
     8221 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     8281 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     8341 tcatgcctcc tc
//
