LOCUS       sequence-446218-        5426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 574, Emission: 596.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5426)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mStrawberry-Cx26-7
FEATURES             Location/Qualifiers
     source          1..5426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     501..520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        775..1078
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1079..1282
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1233..1253
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2096..2806
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mStrawberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILTPNFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVELYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2241..2259)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2509..2528
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2928..3049
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2965..2984)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3019..3038
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3056..3511)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3193..3214)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3405..3424
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3538..3642
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3644..4001
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3644..3835
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3667..3687)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3852..3987
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4036..4830
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4036..4830
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4090..4109)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4700..4719
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5018..5037)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5062..5109
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
       61 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      121 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
      181 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      241 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      301 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      361 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      421 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      481 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      541 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      601 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      661 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
      721 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      781 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      841 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      901 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      961 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     1021 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     1081 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     1141 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     1201 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     1261 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggac
     1321 tcagatctcg agctcaagct tcgaattcgc ggctgcacgc tcctcgggac acagtgccaa
     1381 ccatccagag gacaagatgg attggggcac actacagagc atcctcgggg gtgtcaacaa
     1441 gcactccacc agcattggga aaatctggct cactgtcctc ttcatcttcc gcatcatgat
     1501 cctcgtggtg gccgcgaagg aggtgtgggg agatgagcaa gccgattttg tttgcaacac
     1561 tctccagcct ggctgtaaga atgtgtgcta cgaccactac ttccccatct ctcacatccg
     1621 gctctgggct ctgcagctga tcatggtgtc cacgccggcc ctcctggtag ctatgcacgt
     1681 ggcctaccgg agacacgaaa agaaacggaa gttcatgaag ggagagataa agaacgagtt
     1741 taaggacatc gaagagatca aaacccagaa ggtccgtatc gaagggtccc tgtggtggac
     1801 ctacaccacc agcatcttct tccgggtcat cttcgaagct gtcttcatgt atgtctttta
     1861 catcatgtac aatggcttct tcatgcagcg tctggtgaag tgtaacgcct ggccttgtcc
     1921 caatacagtg gactgcttca tttccaggcc cacagaaaag actgtcttca cggtgttcat
     1981 gatctctgtg tctggaattt gcatcctgct aaacatcaca gagctgtgct atctgttcat
     2041 taggtattgc tcagggaagt ccaaaagacc agtcgcggat ccaccggtgg ctaccatggt
     2101 gagcaagggc gaggagaata acatggccat catcaaggag ttcatgcgct tcaaggtgcg
     2161 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     2221 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     2281 ctgggacatc ctaaccccca acttcaccta cggctccaag gcctacgtga agcaccccgc
     2341 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     2401 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     2461 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     2521 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     2581 gaagggcgag atcaagatga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     2641 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca tcgtcggcat
     2701 caagttggac atcacctccc acaacgagga ctacaccatc gtggaactgt acgaacgcgc
     2761 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaagcgg ccgcgactct
     2821 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     2881 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     2941 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3001 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt
     3061 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     3121 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     3181 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     3241 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     3301 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     3361 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     3421 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     3481 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     3541 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3601 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     3661 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     3721 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     3781 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     3841 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3901 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3961 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     4021 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     4081 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     4141 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     4201 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     4261 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     4321 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     4381 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     4441 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     4501 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     4561 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     4621 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     4681 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     4741 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     4801 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     4861 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     4921 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     4981 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
     5041 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     5101 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     5161 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     5221 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
     5281 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5341 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5401 gagcgtcaga ccccgtagaa aagatc
//
