LOCUS       sequence-446221-       12561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express METTL1-CM gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12561)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 12561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:METTL1-CM_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..12561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gap             1006..1105
                     /estimated_length=100
     polyA_signal    6956..7037
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(7339..8052,8053..8055,8056..8058))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7342..8052,8053..8055,8056..8058))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7384..7405)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7733..7752
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7992..8013
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9239..9255)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9239..9255)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(9310..9328)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9338..9355)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9338..9354)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9347..9369)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9495..9950)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9632..9653)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9844..9863
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9977..10081
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10082..10150,10151..10942)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10300..10319)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11113..11701
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11602..11621
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11855..11872
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11989..12010
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12025..12030,12031..12048,12049..12055)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12063..12079
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12068..12090
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12087..12103
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12087..12103
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12122..12142
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12124..12142
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcactgttct tcaggcactc gtggttacgg gtttttgttt tgcactcttg cacacacatc
       61 tctcggcctc caaaacaaac acacaactgc acgagggctc gaagccccca ccgaaaaaca
      121 aaaacattat gctaatgagg aaggcacagc tgttcccttc ttcactctct ctctcgttct
      181 ttcaacaaca gtcgcctaat ttctctcgtt cctttaaaga gccgccaagc agaaacagtt
      241 aatgcaaata taagcctgcg ggaaataagg agaattttta caaaatccga gcttcccctc
      301 ctgccaaaat gtgatgtgtg actcacgcat tcgatatttg gtgtaatgag agtgaatcat
      361 tctgctccac accgaacccg atgccacaga ccactcagat gcacaccgag gagactattt
      421 taacagcaca ttacatccag actgtcacag atgcattacc aagagtgtga tttcagtgtc
      481 gaatgcaggt cttaaaggaa aatctgttgc gattgcatgt gcgctaacta gacacgacca
      541 acgaatggga acaatctgtg aaatattata caatcacagg caattaaaag atgtttaaag
      601 ttcaatttag ttttaattaa tatgttatat aaaactaatc tgttttaaat gtatatttat
      661 gattttagct ttagctagcc tcttgaccag cagctgaagt gtccggcaag tacctaaaag
      721 ggagaccaca tgggaaagct aggttgctcc cggaagaggt gttagtaaac cagcaggtct
      781 acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa cctgtggtct
      841 gtgtgggtcc taatgcccca gtataaacca gcaggtctac ctgtggtctg tgtgggtcct
      901 aatgccccag tataaaccag caggtcaacc tgtggtctgt gtgggtccta atgccccagt
      961 ataaaccagc aggtctacct gtggtctgtg tgggtcctaa tgcccnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnncagca ggtcaacctg tggtctgtgt gggtcctaat
     1141 gccccagtat aaaccagcag gtctacctgt ggtctgtgtg ggtcctaatg ccccagtata
     1201 aaccagcagg tcaacctgtg gtctgtgtgg gttctaatgc cccagtataa accagcaggt
     1261 caacctgtgg tctgtgtggg tccaaatgcc ccagtacaaa ccagcaggtc aacctgtggt
     1321 ctgtgtgggt cctaatgccc cagtataaac cagcaggtca acctgtggtc tgtgtgggtc
     1381 ctaatgcccc ggtacaaacc agcatgtcaa cctgtggtct gtgtgggtcc taatgccccg
     1441 gtataaacca gcagatcaac ctgtggtctg tgtgggtcct aatgccccag tataaaccag
     1501 cagatcaacc tgtggtctgt gtgggtccta aagtcccagg ataaaccagc atgtcaacct
     1561 gtggtctgtg tgggtcctaa tgccccggta taaaccagca gatcaacctg tggtctgtgt
     1621 gggtcctaat gccccaggat aaaccagcag gtcaacctgt ggtctgtgtg ggtcctaatg
     1681 ccccagtata aaccagcagg tcaacctgtg gtctgtgtgg gtcctaatgc cccagtatat
     1741 tgatggagac tctatactgc tcggtgagct ccatctttcg gataagatgt taaaacaagg
     1801 tcccgactct ctgtggtggt aaaaaattcc caggatgtcc ttcctaccct ggcatcctgg
     1861 acaaatttgc tcactgtcct ctgtccatca tggcctccta accatcccca tatcataatt
     1921 ggctttatca ctctagctgg ggccgaaatc gcatacttcc attttatgag gtacgctaaa
     1981 atcagtatgc gagccaagta atatgtccga atacttccgg cgagattcta gagtgtgcat
     2041 tcgatgcacg ctacgctatt ccatgatgcc ccgtgagaga attcttgaat cggagagaag
     2101 caacgcaact catgcgggta ggttacgtga cgatgacata ttggcggatg tagtacgtcc
     2161 aagttccatt catactgctc acattcatat tttatagaat gttcttttcc cacagccaag
     2221 tagtaagttt aaattcaaat acagtaccta ctaagtagta gtagacggtt tcggacgcag
     2281 cccctgtctc ctctccacca atatactggt gtgtagtgtg cggtctggtg caatatggct
     2341 gccatcgcgt catccaggtg gatgctgcac actggtggtt gatgaggaga ttttgcccaa
     2401 aaaagtgttt tgagtttcca gaaaagcgcg atataaatgt aaggaattat cattagctta
     2461 ttataatgga actttctgga taacttgtgc cagagctagg ggccattaaa actttgccct
     2521 ggggtacctc agggagctgc aaggaagtgc tatagggcta atgaaatgtt tgggggaaaa
     2581 aaaaaaattt tataagtaaa aataaaacta aaataattaa taatttcatt gttgaaaatt
     2641 atagtaaaat tgataaacaa gataagcagc attagtataa aaaactaaaa acaaacgtaa
     2701 tttaaaataa ttaaattgtt ttaaataaat tatacaaaat aatgtttaca acgacacggt
     2761 ggtgcagtag gtagtgctgt cgccagcaaa gcaagaaggt cgctggttcg agcctcagct
     2821 ggatcacttg atgtttctgt gtggagcatt cgcgtgcgtt taaccccaga ttaataaaga
     2881 gactaagccg aaaagaaaat aaatgtatga aaatgtttac gtaaaatgta aataaatgca
     2941 tgattaataa aatatatgaa ttttaaagat tgaggtagaa ttgttttatt attcacacat
     3001 ttggttattt ttaatcagtg acaacttgtg atgctcttta tattgtttat tacgctactt
     3061 tgaatattca gtctcaaatt gcatgtaaat acgacttcaa agcagaatta gtttaattta
     3121 attgcctgta aacagtgttg tactttgatg gtgattgtct gataataata tgatttcact
     3181 atttaaaatt tgaaaaaaaa aaatctgaaa aatactcacc ggccacttta ttaggtacac
     3241 ctgtctaact gcttgttaac gtaaatttct aatcagccaa tcaccaggca gcaactcagt
     3301 gcatttaggc atgtagacat ggtcaagacg atctgctgca gttcaaagcg agcatcagaa
     3361 tggagaagca aggtgattta agtgacttta aacgtgacgt gtttgttggt gccagacggg
     3421 ctggtctgag tatttcagaa actgctgatt tactggggtt ttcacacaca aacatctctg
     3481 gggtttacag agaatggtcc aaaaaaagat aatatccagt gagcggcagt tctgtggggg
     3541 caaatgtctt gttgatgcca gaggtcagag gagaatggcc tgactggttc cagctgatag
     3601 aaaggcaaca gtaattcaaa taaccactcg ttagaactga ggcatgcaga agagcacctt
     3661 tgaacacaac atgtcgaacc ttgaggcggg tgggctacag cagctcaaga ccacatcggg
     3721 tgccactcct gtcagctaag aacaggaaac tgaggctaca attcgcacag gctcaacata
     3781 agtagacaat agaagattga acattatgtt gcctggtcag atggatacat tctaccttgt
     3841 atcaacagtt caggctggtg atggtggtgt aatggtgtgg gggatatatt cttggcacac
     3901 tttgggccca ttagtaccaa ttaaccatcg tgtcaacgcc acagcctaac tgagtattgt
     3961 tgctgaccat gttcatttct ttttgaccac agtgtaccca tcttctgatg gctacttcct
     4021 gcaggataat gcaccatgtc ataaagcgtg aatgatctca gactggtttc ttgaacatga
     4081 caatgagttc actgtgctcg aatgacctcc acagtcacca gatctcaatc caatagagcg
     4141 cctttggggt gtggtagaat ggaagattcg catcctggat gtgcagccga caaatctgca
     4201 tgatgctagc atgtcaatat ggaccaaaat ctctgaggaa ttttccagta cctggttgaa
     4261 atgcaaaagg aagggaatct atgctaaggc aaaaagaggt ccaacccagt actagtgggg
     4321 tgtacataat aaagtggccg gtgagtgtat attgttaagg atatctagat aatccaaata
     4381 atttacagca ctatttaaaa aaaaaacttt atgtcaccaa gtctgcattt atttgatcac
     4441 aattacagtc gaaaaagcaa tatcgggata cattgtttcc atttaaaata actatattat
     4501 attttaaata tttttgacaa tgttatttct ttaatgccaa agctaaattg tattcaatct
     4561 gaattttagt gtcacatgat ccttcagaaa atattaacac attgctaaaa atgtatatta
     4621 ttatcaattt ggattttttt tttttttttg tatattaacc tatgctattg gttactatac
     4681 aaaagtatta ctgtgaatat atgcatatac agaaccgtat caagcgcggg gggtggttgg
     4741 gttatgtccc catttggaaa gacctggaaa cattcataat gactaaaatt gcacggaaaa
     4801 atataatatc tatgagtatt gctgatcagc agcacaaaac cagaactcca tattaataaa
     4861 cagccaatga tgcagtgagt ttgtgcatgt tttcattcaa actcgtgtga ttgtgctcaa
     4921 acacctgttg tgagcagcag ttgcaaacag taaacctcaa gaggagccag tcaaccttca
     4981 caaacactca acctctggct cactcactaa acaattcgcg ttaatatcta aaataaaaat
     5041 ttccagcacc aaacagctct cagatttcgg agtacactga ccaagaggta agaagtaacg
     5101 ttacattact aggacgacta ttgtaagact agatatgtaa tatttggaag cgtacgtgtt
     5161 ttaagtgaca tatagagtag aggaactgat ggaaatacga tttgcataat gtaaaatgat
     5221 atatattaaa atataggcta cagtaggtaa cgtggtttac atataacgtt actgtgttaa
     5281 tgtagtgctc agttactcgt gttgacagtt attattagtg cttgctaatt tgtttgttaa
     5341 tgcttctctt gcgtatttga ttaccttcag gcgaatctaa aacatatcag atgtagaaac
     5401 ggcagaaaaa tgttgaagaa aatacgagaa tagtagcttt tggagcacat agaaaagtac
     5461 atcagataaa gcctaccgtt taaacgcaga cgccgctctc gcactgaatg ctgggtactg
     5521 tagtccttat attgtatgct taatgtgcgt ctcaacggga aactagagtt aggaaaacta
     5581 caatttcctg tggaaaatgt ttctgttttc ggggaaacag cgaaagaaag atatggttcc
     5641 gcgttttttt cttactttga ataataaaat aagacctaac tacacacttc gcttactctt
     5701 atgctaattt gaagcactag cattctttga tacaatgata atcatttaaa catcacagtt
     5761 atactacaaa tactatggtt aaactatgtc tagtgtagtg taacactatg gttgccattg
     5821 ttatctttaa gtttaagtat tatctgtggt aaagccatgg ttggttttta taagaggtaa
     5881 taaaattaac attgtacaat taaatgacta taaattaatg ttttttttta ttttttttac
     5941 acagctacct tcaaaaaggg atccatggca gccgagactc ggaacgtggc cggagcagag
     6001 gccccaccgc cccagaagcg ctactaccgg caacgtgctc actccaaccc catggcggac
     6061 cacacgctgc gctaccctgt gaagccagag gagatggact ggtctgagct atacccagag
     6121 ttcttcgctc cactcactca aaatcagagc cacgatgacc caaaggataa gaaagaaaag
     6181 agagctcagg cccaagtgga gtttgcagac ataggctgtg gctatggtgg cctgttagtg
     6241 gaactgtcac cgctgttccc agacacactt attctgggtc tggagatccg ggtgaaggtc
     6301 tcagactatg tacaagaccg gattcgggcc ctacgcgcag ctcctgcagg tggcttccag
     6361 aacatcgcct gtctccgtag caatgccatg aagcaccttc ctaacttctt ctacaagggc
     6421 cagctgacaa agatgttctt cgccttcccc gccccacatt tcaagcggac aaagcacaag
     6481 tggcgaatca tcagtcccac cctgctagca gaatatgcct acgtgctaag agttgggggg
     6541 ctggtgtata ccataaccga tgtgctggag ctacacgact ggatgtgcac tcatttcgaa
     6601 gagcacccac tgtttgagcg tgtgcctctg gaggacctga gtgaagaccc cgttgtggga
     6661 catctaggca cctcaactga ggaggggaag aaagttctac gtaatggagg gaagaatttc
     6721 ccagccatct tccgaagaat acaagatccc gtcctccagg cagtgacctc ccaaaccagc
     6781 ctgcctggtc actgaatcga tgatgatcca gacatgataa gatacattga tgagtttgga
     6841 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     6901 gctttatttg taaccattat aagattgatg agtttggaca aaccacaact agaatgcagt
     6961 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     7021 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     7081 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     7141 atcctctaga tcagatctct tgtttattgc agcttataat ggttacaaat aaagcaatag
     7201 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7261 actcatcaat gtatcttatc atgtctggat ctacgtaata cgactcacta tagttctaga
     7321 ggctcgagag gggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc
     7381 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
     7441 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
     7501 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
     7561 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
     7621 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
     7681 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
     7741 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
     7801 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta
     7861 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
     7921 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
     7981 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
     8041 ttcgcccttg ctcaccatgg tcactgtctg ctttgctgtt ggtctgggct cctgggtcac
     8101 tggcttacta atggagtctt tatgtatgag gactcttatc aattgttctt ctataaaggt
     8161 ctgcagtgtt tctgttcgtc ccctacatgg acacccagag cctcctaaat acaggagccc
     8221 tgataactgc acaagtgctc agattccagc agggtggaaa tgagataaag tgtgcagatg
     8281 gggaggggga cgtgaatgag agatttgagg gatgaaaagg atggatgaac gcattgaaaa
     8341 tagccccttt cacaaaataa taccagtaaa ttgtcatata attaatgacc agacctttac
     8401 ctgtaaatga tgtgatgttc atatacagaa gaatgccaac aatttacaga tgattttaca
     8461 aattcttaat tttccttttc taaatggatt tgttcacaca ttatctcttc aaagcaattg
     8521 aattttctgg aaaagattgg ctgtgtgtgt aaaaggtgct actgaggaat ttaatgtgac
     8581 atggaatgaa gcaaacagca gcctaagaca ggatggcagg aaaaatgtca catgtttaaa
     8641 ataagagtga aaccaacgac ctggacttat agagactgtg tgtccttagg caaagctctt
     8701 gtacaaacag tgcttgtgac tgtgaaattc ggggtttgcc tggattgtgt taaatgtgtg
     8761 cttggatgta tcataaaaaa gaatcggttc tctgtatttg acattttctg taatttatct
     8821 aaagttacaa ctggctatgc ctgattttta tcactattga atcaatcatt ttaaagaatg
     8881 ttaagtctgc tgatttggag agctcattta catttatttt gaatgtctct tatttaacac
     8941 aactgattta agctttagat ctgcgaagat acggccacgg gtgctcttga tcctgtggct
     9001 gattttggac tgtgctgctc gcagctgctg atgaatcaca tacttcctcc attttcttcc
     9061 actgattgac tgttataatt tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat
     9121 agatgtgaca tgattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     9181 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagatcaga
     9241 tccactagtt ctagagcggc cattaccctg ttatccctag gccgccaccg cggtggagct
     9301 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     9361 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     9421 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     9481 tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     9541 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     9601 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     9661 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     9721 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     9781 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     9841 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     9901 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     9961 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    10021 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    10081 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    10141 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    10201 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    10261 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    10321 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    10381 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    10441 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    10501 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    10561 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    10621 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    10681 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    10741 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    10801 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    10861 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    10921 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    10981 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    11041 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    11101 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    11161 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    11221 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    11281 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    11341 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    11401 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    11461 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    11521 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    11581 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    11641 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    11701 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    11761 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    11821 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    11881 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    11941 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    12001 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    12061 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
    12121 cgcaattaac cctcactaaa gggaacaaaa gctgggtacc gggccccccc tcgaattacc
    12181 ctgttatccc tatcgagcaa aatcaatcct aatgtaaaat agtatacgtt catcattata
    12241 atgcaaacac tgtagaactg ttgtgttgtg ccttgcattt cattctcagt ttcttatcgt
    12301 ctgtctcttt ttacgacaga gctagaaaaa actagccttc atttgccatc gccgtatgca
    12361 aatccactcc agaactcaac aggtttgttc tgcggcgggt gtaagtgtga acaccggcat
    12421 gtaagtgcgc ttgagcagga gaataaagcg ctcagagaca ccggaggaat cctttatttt
    12481 gcgttgcgcc tcaggggatt cgctgagcct tctcttcatg gccctgctct tcagacacag
    12541 gtgctccgct gtttgtgttt t
//
