LOCUS       sequence-446228-       11353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Synaptotagmin1-pHluorin for optical monitoring of 
            synaptic vesicle cycling in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11353)
  AUTHORS   Pascual-Caro C, de Juan-Sanz J
  TITLE     Monitoring of activity-driven trafficking of endogenous synaptic 
            proteins through proximity labeling.
  JOURNAL   PLoS Biol. 2024 Oct 28;22(10):e3002860. doi: 
            10.1371/journal.pbio.3002860. eCollection 2024 Oct.
  PUBMED    39466808
REFERENCE   2  (bases 1 to 11353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2024-10-28"; volume: "22(10):e3002860. doi"; pages: "
            10.1371/journal.pbio.3002860. eCollection 2024 Oct"
COMMENT     Sequence Label: CaMKII-pHluorin-Synaptotagmin1
FEATURES             Location/Qualifiers
     source          1..11353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3890
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      3900..3909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4299..4318)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    6045..6633
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6098..6118)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6516..6527)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6636..6652)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6637..6653)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7158..7338
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7364..7381)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7370..7594
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7640..8068
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7727..7746)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7937..7958
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8077..8097)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8082..8411
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8262..8397
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8324..8343
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8459..8506
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8525..8899
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9029..9150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9066..9085)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9120..9139
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9199..9215)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9199..9215)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9212..9234)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9223..9239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9247..9253,9254..9271,9272..9277))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9292..9313
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9430..9447)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9601..10189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9681..9700)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10360..11151,11152..11220))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10983..11002
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11221..11325)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt
     2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag
     2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat
     2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca
     2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg
     2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga
     2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg
     3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga
     3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac
     3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg
     3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa
     3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt
     3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga
     3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc
     3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa
     3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag
     3541 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac
     3601 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag
     3661 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac
     3721 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg
     3781 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt
     3841 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggatccg
     3901 ccaccatgga ctctaagggc agcagccaga agggctccag actgctgctg ttgctggtgg
     3961 tgtctaacct gctgctctgt cagggcgtcg tgtctggctc taaaggcgag gaactgttca
     4021 ccggcgtggt gcccattctg gtggaactgg atggggacgt gaacggccac aagttctctg
     4081 tgtctggcga aggcgaaggg gacgccacat acggaaagct gaccctgaag ttcatctgca
     4141 ccaccggcaa gctgcctgtg ccttggccta cactggtcac cacactgaca tacggcgtgc
     4201 agtgcttcag cagatacccc gaccatatga agaggcacga cttcttcaag agcgccatgc
     4261 ctgagggcta cgtgcaagag aggaccatct tctttaagga cgacggcaac tacaagacca
     4321 gggccgaagt gaagttcgag ggcgacaccc tggtcaacag gatcgagctg aagggaatcg
     4381 acttcaaaga ggacggcaac atcctgggac acaagttgga gtacaactac aacgatcacc
     4441 aggtgtacat catggccgac aagcagaaga acggcatcaa ggccaacttc aagatccggc
     4501 acaacatcga ggacggcgga gtgcagctgg ctgatcacta ccagcagaac acccctatcg
     4561 gcgacggacc tgtgctgctg cctgataacc actacctgtt caccacaagc accctgagca
     4621 aggaccccaa cgagaagagg gaccacatgg tgctgctgga atttgtgacc gccgctggca
     4681 tcacacacgg catggacgag ctgtacaaag agttcagcgg cggctctggc ggaagcggtg
     4741 gaagcggagg aaccggtgtt tctgcttctc accctgaggc tctggccgct cctgtgacaa
     4801 cagtggctac tctggtgccc cacaacgcca cagaaccagc ttctccaggc gagggcaaag
     4861 aggatgcctt cagcaagctg aagcaaaagt ttatgaacga gctgcacaag atccctctgc
     4921 caccttgggc tctgatcgct atcgccattg tggctgtcct gctggtcgtg acctgctgtt
     4981 tctgcgtgtg caagaagtgc ctgttcaaga agaagaacaa gaagaaaggc aaagagaaag
     5041 gcggcaagaa cgctatcaac atgaaggacg tgaaggacct gggcaagacc atgaaggatc
     5101 aggccctgaa ggacgatgac gccgagacag gactgaccga cggcgaggaa aaagaggaac
     5161 ccaaagagga agaaaagctg gggaagctgc agtacagcct ggactacgac ttccagaaca
     5221 accagctgct cgtgggcatc atccaggctg ctgaactgcc tgctctggac atgggcggaa
     5281 caagcgaccc ttacgtgaag gtgttcctgc tgccagacaa gaaaaagaaa ttcgagacaa
     5341 aggtgcaccg caagacactg aaccccgtgt tcaacgagca gttcaccttc aaggtaccct
     5401 acagcgaact cggcggaaag accctcgtga tggccgtgta cgacttcgac aggttctcca
     5461 agcacgacat catcggcgag ttcaaggtgc ccatgaacac cgtggacttc ggccacgtga
     5521 cagaggaatg gcgggatctg cagagcgccg agaaagagga acaagagaaa ctgggcgaca
     5581 tctgcttcag cctgagatac gtgccaaccg ccggcaaact gaccgtggtc atcctggaag
     5641 ccaagaacct gaagaaaatg gacgtcggcg gcctgtctga tccctacgtg aagatccacc
     5701 tgatgcagaa cggcaagcgg ctgaaaaaga agaaaaccac catcaagaaa aacacgctga
     5761 acccctacta caatgagtcc ttcagcttcg aggtgccctt cgagcagatc cagaaagtgc
     5821 aggtcgtggt caccgtgctg gattacgaca agatcggaaa gaacgacgcc atcggcaagg
     5881 tgttcgtggg ctacaactct acaggcgccg agctgagaca ttggagcgac atgctggcta
     5941 accccaggcg gcctattgct cagtggcaca cactgcaggt cgaggaagag gtggacgcta
     6001 tgctggccgt gaagaagtga gaattcgata tcaagcttat cgataatcaa cctctggatt
     6061 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6121 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6181 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6241 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6301 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6361 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6421 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     6481 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6541 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6601 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag
     6661 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     6721 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     6781 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6841 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     6901 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     6961 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     7021 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     7081 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     7141 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7201 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7261 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7321 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     7381 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     7441 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     7501 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     7561 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     7621 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     7681 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7741 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     7801 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7861 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     7921 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7981 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     8041 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     8101 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     8161 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     8221 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     8281 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     8341 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8401 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     8461 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     8521 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     8581 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     8641 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     8701 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     8761 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     8821 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     8881 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
     8941 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     9001 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     9061 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9121 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     9181 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9241 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9301 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9361 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9421 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9481 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9541 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9601 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9661 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9721 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9781 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9841 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9901 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9961 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    10021 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    10081 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10141 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10201 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10261 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10321 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10381 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10441 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10501 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10561 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10621 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10681 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10741 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10801 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10861 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10921 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10981 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    11041 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    11101 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11161 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11221 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11281 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11341 agtgccacct gac
//
