LOCUS       sequence-446229-       13752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express AVIL gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13752)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 13752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:AVIL_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..13752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gap             1142..1241
                     /estimated_length=100
     CDS             complement(join(8529..9242,9243..9245,9246..9248))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8532..9242,9243..9245,9246..9248))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8574..8595)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8923..8942
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9182..9203
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10429..10445)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10429..10445)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(10500..10518)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10528..10545)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10528..10544)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10537..10559)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(10685..11140)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10822..10843)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11034..11053
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        11167..11271
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11272..11340,11341..12132)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11490..11509)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12303..12891
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12792..12811
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13045..13062
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13179..13200
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13215..13220,13221..13238,13239..13245)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13253..13269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13258..13280
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13277..13293
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13277..13293
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13312..13332
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13314..13332
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cactgttctt caggcactcg tggttacggg tttttgtttt gcactcttgc acacacatct
       61 ctcggcctcc aaaacaaaca cacaactgca cgagggctcg aagcccccac cgaaaaacaa
      121 aaacattatg ctaatgagga aggcacagct gttcccttct tcactctctc tctcgttctt
      181 tcaacaacag tcgcctaatt tctctcgttc ctttaaagag ccgccaagca gaaacagtta
      241 atgcaaatat aagcctgcgg gaaataagga gaatttttac aaaatccgag cttcccctcc
      301 tgccaaaatg tgatgtgtga ctcacgcatt cgatatttgg tgtaatgaga gtgaatcatt
      361 ctgctccaca ccgaacccga tgccacagac cactcagatg cacaccgagg agactatttt
      421 aacagcacat tacatccaga ctgtcacaga tgcattacca agagtgtgat ttcagtgtcg
      481 aatgcaggtc ttaaaggaaa atctgttgcg attgcatgtg cgctaactag acacgaccaa
      541 cgaatgggaa caatctgtga aatattatac aatcacaggc aattaaaaga tgtttaaagt
      601 tcaatttagt tttaattaat atgttatata aaactaatct gttttaaatg tatatttatg
      661 attttagctt tagctagcct cttgaccagc agctgaagtg tccggcaagt acctaaaagg
      721 gagaccacat gggaaagcta ggttgctccc ggaagaggtg ttagtaaacc agcaggtcta
      781 cctgtggtct gtgtgggtcc taatgcccca gtataaacca gcaggtcaac ctgtggtctg
      841 tgtgggtcct aatgccccag tataaaccag caggtctacc tgtggtctgt gtgggtccta
      901 atgccccagt ataaaccagc aggtcaacct gtggtctgtg tgggtcctaa tgccccagta
      961 taaaccagca ggtctacctg tggtctgtgt gggtcctaat gccccagtat aaaccagcag
     1021 gtcaacctgt ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg tctacctgtg
     1081 gtctgtgtgg gtcctaatgc cccagtataa accagcaggt caacctgtgg tctgtgtggg
     1141 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncctaatgcc ccagtataaa
     1261 ccagcaggtc tacctgtggt ctgtgtgggt cctaatgccc cagtataaac cagcaggtca
     1321 acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa cctgtggtct
     1381 gtgtgggtcc taatgcccca ggataaacca gcaggtcaac ctgtggtctg tgtgggtcct
     1441 aatgccccag tataaaccag caggtcaacc tgtggtctgt gtgggtccta atgccccagt
     1501 atattgatgg agactctata ctgctcggtg agctccatct ttcggataag atgttaaaac
     1561 aaggtcccga ctctctgtgg tggtaaaaaa ttcccaggat gtccttccta ccctggcatc
     1621 ctggacaaat ttgctcactg tcctctgtcc atcatggcct cctaaccatc cccatatcat
     1681 aattggcttt atcactctag ctggggccga aatcgcatac ttccatttta tgaggtacgc
     1741 taaaatcagt atgcgagcca agtaatatgt ccgaatactt ccggcgagat tctagagtgt
     1801 gcattcgatg cacgctacgc tattccatga tgccccgtga gagaattctt gaatcggaga
     1861 gaagcaacgc aactcatgcg ggtaggttac gtgacgatga catattggcg gatgtagtac
     1921 gtccaagttc cattcatact gctcacattc atattttata gaatgttctt ttcccacagc
     1981 caagtagtaa gtttaaattc aaatacagta cctactaagt agtagtagac ggtttcggac
     2041 gcagcccctg tctcctctcc accaatatac tggtgtgtag tgtgcggtct ggtgcaatat
     2101 ggctgccatc gcgtcatcca ggtggatgct gcacactggt ggttgatgag gagattttgc
     2161 ccaaaaaagt gttttgagtt tccagaaaag cgcgatataa atgtaaggaa ttatcattag
     2221 cttattataa tggaactttc tggataactt gtgccagagc taggggccat taaaactttg
     2281 ccctggggta cctcagggag ctgcaaggaa gtgctatagg gctaatgaaa tgtttggggg
     2341 aaaaaaaaaa attttataag taaaaataaa actaaaataa ttaataattt cattgttgaa
     2401 aattatagta aaattgataa acaagataag cagcattagt ataaaaaact aaaaacaaac
     2461 gtaatttaaa ataattaaat tgttttaaat aaattataca aaataatgtt tacaacgaca
     2521 cggtggtgca gtaggtagtg ctgtcgccag caaagcaaga aggtcgctgg ttcgagcctc
     2581 agctggatca cttgatgttt ctgtgtggag cattcgcgtg cgtttaaccc cagattaata
     2641 aagagactaa gccgaaaaga aaataaatgt atgaaaatgt ttacgtaaaa tgtaaataaa
     2701 tgcatgatta ataaaatata tgaattttaa agattgaggt agaattgttt tattattcac
     2761 acatttggtt atttttaatc agtgacaact tgtgatgctc tttatattgt ttattacgct
     2821 actttgaata ttcagtctca aattgcatgt aaatacgact tcaaagcaga attagtttaa
     2881 tttaattgcc tgtaaacagt gttgtacttt gatggtgatt gtctgataat aatatgattt
     2941 cactatttaa aatttgaaaa aaaaaaatct gaaaaatact caccggccac tttattaggt
     3001 acacctgtct aactgcttgt taacgtaaat ttctaatcag ccaatcacca ggcagcaact
     3061 cagtgcattt aggcatgtag acatggtcaa gacgatctgc tgcagttcaa agcgagcatc
     3121 agaatggaga agcaaggtga tttaagtgac tttaaacgtg acgtgtttgt tggtgccaga
     3181 cgggctggtc tgagtatttc agaaactgct gatttactgg ggttttcaca cacaaacatc
     3241 tctggggttt acagagaatg gtccaaaaaa agataatatc cagtgagcgg cagttctgtg
     3301 ggggcaaatg tcttgttgat gccagaggtc agaggagaat ggcctgactg gttccagctg
     3361 atagaaaggc aacagtaatt caaataacca ctcgttagaa ctgaggcatg cagaagagca
     3421 cctttgaaca caacatgtcg aaccttgagg cgggtgggct acagcagctc aagaccacat
     3481 cgggtgccac tcctgtcagc taagaacagg aaactgaggc tacaattcgc acaggctcaa
     3541 cataagtaga caatagaaga ttgaacatta tgttgcctgg tcagatggat acattctacc
     3601 ttgtatcaac agttcaggct ggtgatggtg gtgtaatggt gtgggggata tattcttggc
     3661 acactttggg cccattagta ccaattaacc atcgtgtcaa cgccacagcc taactgagta
     3721 ttgttgctga ccatgttcat ttctttttga ccacagtgta cccatcttct gatggctact
     3781 tcctgcagga taatgcacca tgtcataaag cgtgaatgat ctcagactgg tttcttgaac
     3841 atgacaatga gttcactgtg ctcgaatgac ctccacagtc accagatctc aatccaatag
     3901 agcgcctttg gggtgtggta gaatggaaga ttcgcatcct ggatgtgcag ccgacaaatc
     3961 tgcatgatgc tagcatgtca atatggacca aaatctctga ggaattttcc agtacctggt
     4021 tgaaatgcaa aaggaaggga atctatgcta aggcaaaaag aggtccaacc cagtactagt
     4081 ggggtgtaca taataaagtg gccggtgagt gtatattgtt aaggatatct agataatcca
     4141 aataatttac agcactattt aaaaaaaaaa ctttatgtca ccaagtctgc atttatttga
     4201 tcacaattac agtcgaaaaa gcaatatcgg gatacattgt ttccatttaa aataactata
     4261 ttatatttta aatatttttg acaatgttat ttctttaatg ccaaagctaa attgtattca
     4321 atctgaattt tagtgtcaca tgatccttca gaaaatatta acacattgct aaaaatgtat
     4381 attattatca atttggattt tttttttttt tttgtatatt aacctatgct attggttact
     4441 atacaaaagt attactgtga atatatgcat atacagaacc gtatcaagcg cggggggtgg
     4501 ttgggttatg tccccatttg gaaagacctg gaaacattca taatgactaa aattgcacgg
     4561 aaaaatataa tatctatgag tattgctgat cagcagcaca aaaccagaac tccatattaa
     4621 taaacagcca atgatgcagt gagtttgtgc atgttttcat tcaaactcgt gtgattgtgc
     4681 tcaaacacct gttgtgagca gcagttgcaa acagtaaacc tcaagaggag ccagtcaacc
     4741 ttcacaaaca ctcaacctct ggctcactca ctaaacaatt cgcgttaata tctaaaataa
     4801 aaatttccag caccaaacag ctctcagatt tcggagtaca ctgaccaaga ggtaagaagt
     4861 aacgttacat tactaggacg actattgtaa gactagatat gtaatatttg gaagcgtacg
     4921 tgttttaagt gacatataga gtagaggaac tgatggaaat acgatttgca taatgtaaaa
     4981 tgatatatat taaaatatag gctacagtag gtaacgtggt ttacatataa cgttactgtg
     5041 ttaatgtagt gctcagttac tcgtgttgac agttattatt agtgcttgct aatttgtttg
     5101 ttaatgcttc tcttgcgtat ttgattacct tcaggcgaat ctaaaacata tcagatgtag
     5161 aaacggcaga aaaatgttga agaaaatacg agaatagtag cttttggagc acatagaaaa
     5221 gtacatcaga taaagcctac cgtttaaacg cagacgccgc tctcgcactg aatgctgggt
     5281 actgtagtcc ttatattgta tgcttaatgt gcgtctcaac gggaaactag agttaggaaa
     5341 actacaattt cctgtggaaa atgtttctgt tttcggggaa acagcgaaag aaagatatgg
     5401 ttccgcgttt ttttcttact ttgaataata aaataagacc taactacaca cttcgcttac
     5461 tcttatgcta atttgaagca ctagcattct ttgatacaat gataatcatt taaacatcac
     5521 agttatacta caaatactat ggttaaacta tgtctagtgt agtgtaacac tatggttgcc
     5581 attgttatct ttaagtttaa gtattatctg tggtaaagcc atggttggtt tttataagag
     5641 gtaataaaat taacattgta caattaaatg actataaatt aatgtttttt tttatttttt
     5701 ttacacagct accttcaaaa agggatccgt cctggaaaga caagcatcat gcctctgacc
     5761 agtgccttca gggctgtgga caacgaccct gggatcattg tctggagaat agagaaaatg
     5821 gagctggcgc tggtgcctgt gagcgcccac ggcaacttct atgaggggga ctgctacgtc
     5881 atcctctcga cccggagagt ggccagtctc ctatcccagg acatccactt ctggatcggg
     5941 aaggactcct cccaggatga gcaaagctgc gcagccatat ataccacaca gctggacgac
     6001 tacctgggag gcagccctgt gcagcaccga gaggtccagt accatgagtc agacactttc
     6061 cgtggctact tcaagcaggg catcatctac aagcaggggg gtgtcgcctc tgggatgaag
     6121 cacgtggaga ccaataccta cgacgtgaag cggctgctac atgtgaaagg gaaaagaaac
     6181 atcagggcta ccgaggtgga aatgagctgg gacagtttca accgaggtga tgtcttcttg
     6241 ctggaccttg ggaaagtcat catccaatgg aatggcccag agagcaacag tggggagcgc
     6301 ctgaaggcta tgcttctggc aaaggatatt cgagacaggg agcgaggggg ccgtgctgaa
     6361 ataggagtga tcgagggaga caaggaggca gccagcccag agctgatgaa ggtccttcag
     6421 gacacccttg gccgacgctc cattatcaag cctacagtcc ctgatgagat catagatcag
     6481 aagcagaaat caactatcat gttgtatcat atctcagatt cagctgggca gctggcagtc
     6541 acagaggtag caacaaggcc tctggtccag gacttactga accatgatga ctgctacatc
     6601 ctggaccaaa gtggaaccaa aatctacgtg tggaaaggaa aaggagccac aaaggctgaa
     6661 aaacaggcag ccatgtctaa agcgctgggc ttcatcaaga tgaagagcta ccccagcagc
     6721 accaatgtgg agaccgtcaa cgatggtgct gagtcggcca tgttcaagca gctgttccag
     6781 aagtggtcag taaaggacca gaccatgggc ctggggaaaa cgttcagcat tggtaaaatt
     6841 gctaaagttt tccaggataa atttgatgtg actctgctac acaccaagcc agaggtagct
     6901 gcccaggaaa gaatggtcga tgatggcaac ggaaaagttg aggtctggag aattgagaac
     6961 ctggagctgg tccctgtgga gtatcaatgg tatggcttct tttatggggg agactgttat
     7021 ctggtcctct acacatacga ggtaaatggg aagccacatc acatcttgta catctggcag
     7081 ggccgccacg cctcacagga tgagctggca gcctcagcat accaggcagt ggaggtggat
     7141 cggcagtttg atggggctgc tgtgcaggtt cgagtcagga tgggaacgga gccacgccac
     7201 ttcatggcca tcttcaaagg gaagctagtt atctttgagg gtgggacttc caggaaggga
     7261 aatgccgagc ctgaccctcc agtaagactc ttccaaattc atggaaatga caaatctaac
     7321 accaaagcag tggaagttcc agcctttgcc tcctccctaa actccaatga tgtctttctg
     7381 ctgcgaactc aggcagagca ctacctgtgg tatggcaagg ggtctagtgg ggatgagcgg
     7441 gcaatggcta aggagctggc cagccttctc tgtgatggca gcgagaacac tgtggccgag
     7501 ggccaggagc cagccgagtt ctgggaccta ctgggaggga aaactcccta tgccaatgat
     7561 aaaagacttc agcaggaaat cctagatgtc cagtctcgtc tctttgaatg ttccaataag
     7621 accggccaat tcgttgtcac tgagatcaca gacttcaccc aggatgacct gaaccctact
     7681 gacgtgatgc tcctagatac ctgggaccag gtgttcttgt ggattggggc tgaggccaat
     7741 gccacggaga aggagagtgc ccttgccaca gcacagcagt acctgcacac tcaccccagc
     7801 ggccgagatc ccgacacacc aatcctgatc attaagcagg ggtttgagcc tcccatcttc
     7861 acaggctggt tcctagcctg ggaccctaac atttggagtg caggaaaaac atatgaacaa
     7921 ttaaaagaag agctgggaga tgctgctgct atcatgcgaa tcactgctga catgaagaat
     7981 gcaaccctct ccctgaattc taatgacagt gagccaaaat attaccctat agcagttctg
     8041 ttgaaaaacc agaatcagga gctgcctgag gatgtaaacc ctgccaaaaa ggagaattac
     8101 ctctctgaac aggactttgt gtctgtgttt ggcatcacaa gagggcaatt tgcagctctg
     8161 cctggctgga aacagctcca aatgaagaaa gaaaaggggc ttttctaaag caagaaggcc
     8221 tataccatcg atgatgatcc agacatgata agatacattg atgagtttgg acaaaccaca
     8281 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     8341 gtaaccatta taagtattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8401 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8461 gtatcttatc atgtctggat ctacgtaata cgactcacta tagttctaga ggctcgagag
     8521 gggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
     8581 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
     8641 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
     8701 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
     8761 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
     8821 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
     8881 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
     8941 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
     9001 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg
     9061 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
     9121 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
     9181 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ttcgcccttg
     9241 ctcaccatgg tcactgtctg ctttgctgtt ggtctgggct cctgggtcac tggcttacta
     9301 atggagtctt tatgtatgag gactcttatc aattgttctt ctataaaggt ctgcagtgtt
     9361 tctgttcgtc ccctacatgg acacccagag cctcctaaat acaggagccc tgataactgc
     9421 acaagtgctc agattccagc agggtggaaa tgagataaag tgtgcagatg gggaggggga
     9481 cgtgaatgag agatttgagg gatgaaaagg atggatgaac gcattgaaaa tagccccttt
     9541 cacaaaataa taccagtaaa ttgtcatata attaatgacc agacctttac ctgtaaatga
     9601 tgtgatgttc atatacagaa gaatgccaac aatttacaga tgattttaca aattcttaat
     9661 tttccttttc taaatggatt tgttcacaca ttatctcttc aaagcaattg aattttctgg
     9721 aaaagattgg ctgtgtgtgt aaaaggtgct actgaggaat ttaatgtgac atggaatgaa
     9781 gcaaacagca gcctaagaca ggatggcagg aaaaatgtca catgtttaaa ataagagtga
     9841 aaccaacgac ctggacttat agagactgtg tgtccttagg caaagctctt gtacaaacag
     9901 tgcttgtgac tgtgaaattc ggggtttgcc tggattgtgt taaatgtgtg cttggatgta
     9961 tcataaaaaa gaatcggttc tctgtatttg acattttctg taatttatct aaagttacaa
    10021 ctggctatgc ctgattttta tcactattga atcaatcatt ttaaagaatg ttaagtctgc
    10081 tgatttggag agctcattta catttatttt gaatgtctct tatttaacac aactgattta
    10141 agctttagat ctgcgaagat acggccacgg gtgctcttga tcctgtggct gattttggac
    10201 tgtgctgctc gcagctgctg atgaatcaca tacttcctcc attttcttcc actgattgac
    10261 tgttataatt tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca
    10321 tgattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
    10381 gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagatcaga tccactagtt
    10441 ctagagcggc cattaccctg ttatccctag gccgccaccg cggtggagct ccaattcgcc
    10501 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
    10561 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
    10621 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
    10681 atggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
    10741 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
    10801 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
    10861 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
    10921 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
    10981 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
    11041 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
    11101 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
    11161 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
    11221 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
    11281 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
    11341 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
    11401 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
    11461 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
    11521 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
    11581 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
    11641 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
    11701 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
    11761 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
    11821 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    11881 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    11941 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    12001 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    12061 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    12121 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    12181 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    12241 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    12301 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    12361 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    12421 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    12481 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    12541 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    12601 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    12661 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    12721 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    12781 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    12841 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    12901 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    12961 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    13021 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
    13081 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
    13141 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
    13201 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
    13261 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
    13321 cctcactaaa gggaacaaaa gctgggtacc gggccccccc tcgaattacc ctgttatccc
    13381 tatcgagcaa aatcaatcct aatgtaaaat agtatacgtt catcattata atgcaaacac
    13441 tgtagaactg ttgtgttgtg ccttgcattt cattctcagt ttcttatcgt ctgtctcttt
    13501 ttacgacaga gctagaaaaa actagccttc atttgccatc gccgtatgca aatccactcc
    13561 agaactcaac aggtttgttc tgcggcgggt gtaagtgtga acaccggcat gtaagtgcgc
    13621 ttgagcagga gaataaagcg ctcagagaca ccggaggaat cctttatttt gcgttgcgcc
    13681 tcaggggatt cgctgagcct tctcttcatg gccctgctct tcagacacag gtgctccgct
    13741 gtttgtgttt tt
//
