LOCUS       sequence-446230-        7529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression androgen receptor 1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7529)
  AUTHORS   Xiao H, Wang L, Yan S, Ma H, Xu Z, Wang F, Wang J, Tao W, Wang D
  TITLE     Steroid hormone-deprived sex reversal in cyp11a1 mutant XX tilapia 
            experiences an ovary-like stage at molecular level.
  JOURNAL   Commun Biol. 2024 Sep 16;7(1):1154. doi: 10.1038/s42003-024-06853-8.
  PUBMED    39284885
REFERENCE   2  (bases 1 to 7529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2024-09-16"; volume: "7(1):1154. doi"; pages: " 
            10.1038/s42003-024-06853-8"
COMMENT     Sequence Label: pcDNA3.1-ar1
FEATURES             Location/Qualifiers
     source          1..7529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             911..934
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3027..3068
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3078..3095
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3118..3135)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3124..3348
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3394..3822
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3481..3500)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3691..3712
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3831..3851)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3836..4165
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4016..4151
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4078..4097
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4232..5026
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4286..4305)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4896..4915
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5202..5323
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5239..5258)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5293..5312
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5372..5388)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5372..5388)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5385..5407)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5396..5412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5420..5426,5427..5444,5445..5450))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5465..5486
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5603..5620)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5774..6362)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5854..5873)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6533..7324,7325..7393))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7156..7175
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7394..7498)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttatg gattataaag atgatgatga taaatccttt cactcgtttt tggaagaaac
      961 aaactcttta acgatgaatg aaggggatcg attggatact accacctatg cctgtccggc
     1021 ttatgagctc agtaaagcgg tgtcagtgtc tttgggtttg gattcggtgt cttctccgcc
     1081 caataatatg aaccagagct ccagcagcgc cttcgcagaa tgcgactccg gtactgtggc
     1141 ggatagttct cggggagtgc cagagttgag aagggctggg aatatgaact ccgacggcag
     1201 catgtttgtc ctgggtgaca gcagcctcag agaggacgac tttggagaag tgtgccaggg
     1261 catacagcag gtgagctgta tggatctgtt cggatcgggc gaaatggaca gtgcgcaaac
     1321 tgtgacgcgt ggctcggtga tatccagata cgtctgcagg gagtcaaatg tgtttatgaa
     1381 ccccacgccg gagctgcccg cgccccctca ggtgcccggg gtcgtaccct taaaaccacc
     1441 ttcgtcttac tgtgccagtt cagacctata tagggatctc ccaccggaaa tgtggtgcgc
     1501 aaatgagcgc gcatacagcg accgatccca gccacagcca cagaggggag aatcagggga
     1561 acataatttc ttgtgcaaat actgtaattg tgggcaagct cctcgcggaa acaggcagga
     1621 atgccgctgt atctggtacg gtagaggtga ccagggtggc aaaggtggca tgcgagcgac
     1681 ggcacaagga tatggccaaa tgtcaagtta cccaagtgcc attcctcagg ggcagaacac
     1741 tttctccact atcaagagtg agccttcggt gtggatgaat tgcacagatc gcagtttaag
     1801 gcacgaggac ttttttccag gtgtgtatct gtcagaaagg agagtgtgtc aagtgtgcgg
     1861 agatgacgcc tcgggttgtc actatggagc agtcacctgc ggcagctgca aagtattctt
     1921 caagagggcc gctgcaggta agcagaacca cttgtgtgcc agccgaaacg actgcaccat
     1981 cgacaaactg aggcggaaaa actgcgcgtc gtgtcgctta aagagatgct tcatgtcggg
     2041 aatgagcctt aaaggtcgca ggctgaaggg agccggacag gcgagaaatg gagaagagga
     2101 gcaacagcca gctagctggg gacaaggaga aaaagaagag agggcagcga agaaagacgt
     2161 agtcttggag tccggaaatg caggtgtcag ggctcaaggt gcagcttccc aggccctggg
     2221 tgcggccatc cccccacccc tgcactcgtg cctgtccctg ctcagcatcc tacaggctat
     2281 tgagccagct gtggtcaatg ccgggcatga tcctgcccag ccagacagcc ctatgtcctt
     2341 gcttaccagc ctgaacaagc tgggggaacg gcagctggta accgtggtcc gctgggccaa
     2401 ggcaatacca gggtttcggg atctgcacgt ggatgaccag atgtcagtga ttcagctgtc
     2461 atggatggga gtgatggtgt tcgccttagg ctggagatcc tacacgctga ctaacagctc
     2521 tatgctctac tttgctccag acctgatttt caatgaccag cggatgcaag cgtccagcat
     2581 gtatgaacac tgtgtgagga tgaagctact atcccaaagg ctgtgcatgc tcaaggttac
     2641 ccaggaggag ttcctctgca tgaaggccct ggtcctcctc agcatcatgc cagagcaggg
     2701 cccgaagagc cagcattgtt ttgacaaact gcggacctcc tacatcaagg agctagaccg
     2761 tttggccagc caccgcggag agaccacccg cacgcagaga ctgtttcagc tcacgcagct
     2821 gctggaccac ctccagtcgg ttgtgaggaa attacaccag ttcacctacg atctgttcat
     2881 ccaagctcag tccatgcatg tcagcttccc agagatgatc tcggagattg tgagcatcca
     2941 cgtgcccaaa atcctctctg ggatggtcaa gcccatcctt ttccacaacg tggcctagct
     3001 cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg
     3061 attctacgcg taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct
     3121 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     3181 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     3241 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     3301 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     3361 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     3421 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     3481 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3541 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     3601 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3661 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3721 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     3781 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     3841 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3901 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3961 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     4021 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4081 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     4141 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     4201 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     4261 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     4321 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     4381 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     4441 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     4501 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     4561 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     4621 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     4681 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     4741 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     4801 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     4861 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     4921 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     4981 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     5041 ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5101 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5161 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     5221 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5281 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     5341 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5401 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5461 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5521 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5581 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5641 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5701 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5761 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5821 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5881 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5941 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6001 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6061 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6121 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6181 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6241 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6301 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6361 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6421 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6481 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6541 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6601 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6661 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6721 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6781 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6841 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     6901 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     6961 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7021 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7081 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7141 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7201 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7261 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7321 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7381 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7441 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7501 catttccccg aaaagtgcca cctgacgtc
//
