LOCUS       sequence-446274-        9060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector with Tks5 tagged with N-terminal mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9060)
  AUTHORS   Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska 
            J
  TITLE     Dynamic regulation of integrin beta1 phosphorylation supports 
            invasion of breast cancer cells.
  JOURNAL   Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
  PUBMED    40419795
REFERENCE   2  (bases 1 to 9060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4."
COMMENT     Sequence Label: pDEST-mScarlet-SH3PXD2A
FEATURES             Location/Qualifiers
     source          1..9060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             923..1618
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(1056..1074)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1324..1343
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    1640..1664
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5010..5034)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5068..5085)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5074..5298
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5344..5772
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5431..5450)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5641..5662
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5781..5801)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5786..6115
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5966..6101
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6163..6210
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6229..6603
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6733..6854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6770..6789)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6824..6843
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6903..6919)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6903..6919)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6916..6938)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6927..6943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6951..6957,6958..6975,6976..6981))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6996..7017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7134..7151)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7305..7893)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7385..7404)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8064..8855,8856..8924))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8687..8706
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8925..9029)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccatggtgag caagggcgag gcagtgatca aggagttcat
      961 gcggttcaag gtgcacatgg agggctccat gaacggccac gagttcgaga tcgagggcga
     1021 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     1081 ccccctgccc ttctcctggg acatcctgtc ccctcagttc atgtacggct ccagggcctt
     1141 caccaagcac cccgccgaca tccccgacta ctataagcag tccttccccg agggcttcaa
     1201 gtgggagcgc gtgatgaact tcgaggacgg cggcgccgtg accgtgaccc aggacacctc
     1261 cctggaggac ggcaccctga tctacaaggt gaagctccgc ggcaccaact tccctcctga
     1321 cggccccgta atgcagaaga agacaatggg ctgggaagcg tccaccgagc ggttgtaccc
     1381 cgaggacggc gtgctgaagg gcgacattaa gatggccctg cgcctgaagg acggcggccg
     1441 ctacctggcg gacttcaaga ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc
     1501 ctacaacgtc gaccgcaagt tggacatcac ctcccacaac gaggactaca ccgtggtgga
     1561 acagtacgaa cgctccgagg gccgccactc caccggcggc atggacgagc tgtacaagat
     1621 cgatggtggc tctggaggta caagtttgta caaaaaagca gaagggccgt caaggcccac
     1681 catgctcgcc tactgcgtgc aggatgccac cgtggtggac gtggagaagc ggaggaaccc
     1741 ctccaagcac tacgtataca taatcaatgt gacctggtct gactccacct cccagactat
     1801 ctaccggagg tacagcaagt tctttgacct gcagatgcag cttttggata agtttcccat
     1861 tgaaggtggc cagaaggacc ccaagcaaag gatcatcccc ttcctcccag gcaagatcct
     1921 cttccgcaga agccacatcc gggacgtagc tgtgaagaga ctgaagccca tcgatgaata
     1981 ctgccgggca cttgtccggc tgccccccca catctcacag tgtgacgaag tcttccggtt
     2041 cttcgaggct cgacccgagg atgtcaaccc tccaaaagag gactatggca gttccaagag
     2101 gaaatcagtg tggctgtcca gctgggctga gtcgcccaag aaggacgtga caggtgccga
     2161 cgccaccgcc gagcccatga tcctggaaca gtacgtggtg gtgtccaact ataagaagca
     2221 ggagaactcg gagctgagcc tccaggccgg ggaggtggtg gatgtcatcg agaagaacga
     2281 gagcggctgg tggttcgtga gcacttctga ggagcagggc tgggtccctg ccacctacct
     2341 ggaggcccag aatggtactc gggatgactc cgacatcaac acctctaaga ctggagaaga
     2401 ggagaagtat gtcaccgtgc agccttacac cagccaaagc aaggacgaga ttggctttga
     2461 gaagggcgtc acagtggagg tgatccggaa gaatctggaa ggctggtggt atatcagata
     2521 cctgggcaaa gagggctggg cgccagcatc ctacctgaag aaggccaagg atgacctgcc
     2581 aacccggaag aagaacctgg ccggcccagt ggagatcatt gggaacatca tggagatcag
     2641 caacctgctg aacaagaagg cgtctgggga caaggaaact ccaccagccg aaggcgaggg
     2701 ccatgaggcc cccattgcca agaaggagat cagcctgccc atcctctgca atgcctccaa
     2761 tggcagtgcc gtgggcgttc ctgacaggac tgtctccagg ctggcccagg gctctccagc
     2821 tgtggccagg attgcccctc agcgggccca gatcagctcc ccgaacctac ggacaagacc
     2881 tccaccacgc agagaatcca gcctggggtt ccaactgcca aagccaccag agcccccttc
     2941 tgttgaggtg gagtactaca ccattgccga attccagtcg tgcatttccg atggcatcag
     3001 ctttcggggt ggacagaagg cagaggtcat tgataagaac tcaggtggct ggtggtacgt
     3061 gcagatcggt gagaaggagg gctgggcccc cgcatcatac atcgataagc gcaagaagcc
     3121 caacctgagc cgccgcacaa gcacgctgac ccggcccaag gtgcccccgc cagcaccccc
     3181 cagcaagccc aaggaggccg aggagggccc tacgggggcc agtgagagcc aggactcccc
     3241 gcggaagctc aagtatgagg agcctgagta tgacatccct gcattcggct ttgactcaga
     3301 gcctgagctg agcgaggagc ccgtggagga cagagcctca ggggagaggc ggcctgccca
     3361 gccccaccgg ccctcgccgg cctcttctct gcagcgggcc cgcttcaagg tgggtgagtc
     3421 ttcagaggat gtggccctgg aagaggagac catctatgag aatgagggct tccggccata
     3481 tgcagaggac accctgtcag ccagaggctc ctccggggac agcgactccc caggcagctc
     3541 ctcgctgtcc ctgaccagga aaaactcccc caaatcaggc tcccccaagt catcatcact
     3601 cctaaagctc aaggcagaga agaatgccca ggcagaaatg gggaagaacc actcctcagc
     3661 ctccttttcc tcatccatca ccatcaacac cacttgctgc tcctcctctt cctcctcctc
     3721 ctcttccttg tccaaaacca gtggcgacct gaagccccgc tctgcttcgg acgcaggcat
     3781 ccgcggcact cccaaggtca gggcaaagaa ggatgctgat gcgaacgctg ggctgacctc
     3841 ctgtccccgg gccaagccat cggtccggcc caagccattc ctaaaccgag cagagtcgca
     3901 gagccaagag aagatggaca tcagcacttt acggcgccag ctgagaccca caggccagct
     3961 ccgtggaggg ctcaagggct ccaagagtga ggattcggag ctgcccccgc agacggcctc
     4021 cgaggctccc agtgaggggt ctaggagaag ctcatccgac ctcatcaccc tcccagccac
     4081 cactccccca tgtcccacca agaaggaatg ggaagggcca gccacctcgt acatgacatg
     4141 cagcgcctac cagaaggtcc aggactcgga gatcagcttc cccgcgggcg tggaggtgca
     4201 ggtgctggag aagcaggaga gcgggtggtg gtatgtgagg tttggggagc tggagggctg
     4261 ggccccttcc cactatttgg tgctggatga gaacgagcaa cctgacccct ctggcaaaga
     4321 gctggacaca gtgcccgcca agggcaggca gaacgaaggc aaatcagaca gcctggagaa
     4381 gatcgagagg cgcgtccaag cactgaacac cgtcaaccag agcaagaagg ccacgccccc
     4441 catcccctcc aaacctcccg ggggcttcgg caagacctca ggcactccag cggtgaagat
     4501 gaggaacgga gtgcggcagg tggcggtcag gccccagtcg gtgtttgtgt ccccgccacc
     4561 caaggacaac aacctgtcct gcgccctgcg gaggaatgag tcactcacgg ccactgatgg
     4621 cctccgaggc gtccgacgga actcctcctt tagcactgct cgctccgctg ccgccgaggc
     4681 caagggccgc ctggccgaac gggctgccag ccagggttca gactcacccc tactgcccgc
     4741 ccagcgcaac agcatacccg tgtcccctgt gcgccccaag cccatcgaga agtctcagtt
     4801 catccacaat aacctcaaag atgtgtacgt ctctatcgca gactacgagg gggatgagga
     4861 gacagcaggc ttccaggagg gggtgtccat ggaggttctg gagaggaacc ctaatggctg
     4921 gtggtactgc cagatcctgg atggtgtgaa gcccttcaaa ggctgggtgc cttccaacta
     4981 ccttgagaaa aagaactcag gcctcatggg cccagctttc ttgtacaaag tggttgtcta
     5041 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     5101 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     5161 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     5221 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     5281 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     5341 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5401 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5461 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5521 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5581 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5641 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5701 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5761 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     5821 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5881 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5941 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6001 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     6061 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     6121 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     6181 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     6241 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     6301 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     6361 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     6421 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     6481 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     6541 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     6601 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6661 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6721 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6781 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6841 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     6901 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6961 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     7021 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7081 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7141 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7201 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7261 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7321 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7381 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7441 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7501 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7561 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7621 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7681 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7741 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7801 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7861 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7921 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7981 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8041 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8101 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8161 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8221 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8281 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8341 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8401 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8461 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8521 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8581 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8641 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8701 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8761 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8821 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8881 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8941 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9001 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
