LOCUS       sequence-446275-       11941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HMGA2 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11941)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 11941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:HMGA2_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..11941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    6127..6261
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             join(7349..7351,7352..7354,7355..8068)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7349..7351,7352..7354,7355..8065)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7394..7415)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7655..7674)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8002..8023
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8370..8504
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8619..8635)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8619..8635)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(8690..8708)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8718..8735)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8718..8734)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8727..8749)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(8875..9330)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9012..9033)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9224..9243
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9357..9461
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9462..9530,9531..10322)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9680..9699)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10493..11081
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10982..11001
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11235..11252
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11369..11390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11405..11410,11411..11428,11429..11435)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11443..11459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11448..11470
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11467..11483
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11467..11483
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11502..11522
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11504..11522
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcactgttct tcaggcactc gtggttacgg gtttttgttt tgcactcttg cacacacatc
       61 tctcggcctc caaaacaaac acacaactgc acgagggctc gaagccccca ccgaaaaaca
      121 aaaacattat gctaatgagg aaggcacagc tgttcccttc ttcactctct ctctcgttct
      181 ttcaacaaca gtcgcctaat ttctctcgtt cctttaaaga gccgccaagc agaaacagtt
      241 aatgcaaata taagcctgcg ggaaataagg agaattttta caaaatccga gcttcccctc
      301 ctgccaaaat gtgatgtgtg actcacgcat tcgatatttg gtgtaatgag agtgaatcat
      361 tctgctccac accgaacccg atgccacaga ccactcagat gcacaccgag gagactattt
      421 taacagcaca ttacatccag actgtcacag atgcattacc aagagtgtga tttcagtgtc
      481 gaatgcaggt cttaaaggaa aatctgttgc gattgcatgt gcgctaacta gacacgacca
      541 acgaatggga acaatctgtg aaatattata caatcacagg caattaaaag atgtttaaag
      601 ttcaatttag ttttaattaa tatgttatat aaaactaatc tgttttaaat gtatatttat
      661 gattttagct ttagctagcc tcttgaccag cagctgaagt gtccggcaag tacctaaaag
      721 ggagaccaca tgggaaagct aggttgctcc cggaagaggt gttagtaaac cagcaggtct
      781 acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa cctgtggtct
      841 gtgtgggtcc taatgcccca gtataaacca gcaggtctac ctgtggtctg tgtgggtcct
      901 aatgccccag tataaaccag caggtcaacc tgtagtctgt gtgggtccta atgccccagt
      961 ataaaccagc aggtctacct gtggtctgtg tgggtcctaa tgccccagta taaaccaaca
     1021 ggtcaacctg tgatctgtgt gggtcctaat gccccagtat aaaccagcag gtcaacctgt
     1081 ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg tcaacctgtg gtctgtgtgg
     1141 gtcctaatgc cccagtataa accagcaggt caacacctgt ggtctgtgtg ggtcctaatg
     1201 ccccggtaca aaccagcatg tcaacctgtg gtctgtgtgg gtcctaatgc cccagtataa
     1261 accagcaggt caacctgtgg tctgtgtggg tcctaatgcc ccagtataaa ccagcaggtc
     1321 tacctgtggt ctgtgtgggt cctaatgccc cagtataaac cagcaggtca acctgtagtc
     1381 tgtgtgggtc ctaatgcccc agtataaacc agcaggtcta cctgtggtct gtgtgggtcc
     1441 taatgcccca gtataaacca gcaggtcaac ctgtggtctg tgtgggtcct aatgccccag
     1501 tataaaccag caggtcaacc tgtggtctgt gtgggtccta atgccccagt atattgatgg
     1561 agactctata ctgctcggtg agctccatct ttcggataag atgttaaaac aaggtcccga
     1621 ctctctgtgg tggtaaaaaa ttcccaggat gtccttccta ccctggcatc ctggacaaat
     1681 ttgctcactg tcctctgtcc atcatggcct cctaaccatc cccatatcat aattggcttt
     1741 atcactctag ctggggccga aatcgcatac ttccatttta tgaggtacgc taaaatcagt
     1801 atgcgagcca agtaatatgt ccgaatactt ccggcgagat tctagagtgt gcattcgatg
     1861 cacgctacgc tattccatga tgccccgtga gagaattctt gaatcggaga gaagcaacgc
     1921 aactcatgcg ggtaggttac gtgacgatga catattggcg gatgtagtac gtccaagttc
     1981 cattcatact gctcacattc atattttata gaatgttctt ttcccacagc caagtagtaa
     2041 gtttaaattc aaatacagta cctactaagt agtagtagac ggtttcggac gcagcccctg
     2101 tctcctctcc accaatatac tggtgtgtag tgtgcggtct ggtgcaatat ggctgccatc
     2161 gcgtcatcca ggtggatgct gcacactggt ggttgatgag gagattttgc ccaaaaaagt
     2221 gttttgagtt tccagaaaag cgcgatataa atgtaaggaa ttatcattag cttattataa
     2281 tggaactttc tggataactt gtgccagagc taggggccat taaaactttg ccctggggta
     2341 cctcagggag ctgcaaggaa gtgctatagg gctaatgaaa tgtttggggg aaaaaaaaaa
     2401 attttataag taaaaataaa actaaaataa ttaataattt cattgttgaa aattatagta
     2461 aaattgataa acaagataag cagcattagt ataaaaaact aaaaacaaac gtaatttaaa
     2521 ataattaaat tgttttaaat aaattataca aaataatgtt tacaacgaca cggtggtgca
     2581 gtaggtagtg ctgtcgccag caaagcaaga aggtcgctgg ttcgagcctc agctggatca
     2641 cttgatgttt ctgtgtggag cattcgcgtg cgtttaaccc cagattaata aagagactaa
     2701 gccgaaaaga aaataaatgt atgaaaatgt ttacgtaaaa tgtaaataaa tgcatgatta
     2761 ataaaatata tgaattttaa agattgaggt agaattgttt tattattcac acatttggtt
     2821 atttttaatc agtgacaact tgtgatgctc tttatattgt ttattacgct actttgaata
     2881 ttcagtctca aattgcatgt aaatacgact tcaaagcaga attagtttaa tttaattgcc
     2941 tgtaaacagt gttgtacttt gatggtgatt gtctgataat aatatgattt cactatttaa
     3001 aatttgaaaa aaaaaaatct gaaaaatact caccggccac tttattaggt acacctgtct
     3061 aactgcttgt taacgtaaat ttctaatcag ccaatcacca ggcagcaact cagtgcattt
     3121 aggcatgtag acatggtcaa gacgatctgc tgcagttcaa agcgagcatc agaatggaga
     3181 agcaaggtga tttaagtgac tttaaacgtg acgtgtttgt tggtgccaga cgggctggtc
     3241 tgagtatttc agaaactgct gatttactgg ggttttcaca cacaaacatc tctggggttt
     3301 acagagaatg gtccaaaaaa agataatatc cagtgagcgg cagttctgtg ggggcaaatg
     3361 tcttgttgat gccagaggtc agaggagaat ggcctgactg gttccagctg atagaaaggc
     3421 aacagtaatt caaataacca ctcgttagaa ctgaggcatg cagaagagca cctttgaaca
     3481 caacatgtcg aaccttgagg cgggtgggct acagcagctc aagaccacat cgggtgccac
     3541 tcctgtcagc taagaacagg aaactgaggc tacaattcgc acaggctcaa cataagtaga
     3601 caatagaaga ttgaacatta tgttgcctgg tcagatggat acattctacc ttgtatcaac
     3661 agttcaggct ggtgatggtg gtgtaatggt gtgggggata tattcttggc acactttggg
     3721 cccattagta ccaattaacc atcgtgtcaa cgccacagcc taactgagta ttgttgctga
     3781 ccatgttcat ttctttttga ccacagtgta cccatcttct gatggctact tcctgcagga
     3841 taatgcacca tgtcataaag cgtgaatgat ctcagactgg tttcttgaac atgacaatga
     3901 gttcactgtg ctcgaatgac ctccacagtc accagatctc aatccaatag agcgcctttg
     3961 gggtgtggta gaatggaaga ttcgcatcct ggatgtgcag ccgacaaatc tgcatgatgc
     4021 tagcatgtca atatggacca aaatctctga ggaattttcc agtacctggt tgaaatgcaa
     4081 aaggaaggga atctatgcta aggcaaaaag aggtccaacc cagtactagt ggggtgtaca
     4141 taataaagtg gccggtgagt gtatattgtt aaggatatct agataatcca aataatttac
     4201 agcactattt aaaaaaaaaa ctttatgtca ccaagtctgc atttatttga tcacaattac
     4261 agtcgaaaaa gcaatatcgg gatacattgt ttccatttaa aataactata ttatatttta
     4321 aatatttttg acaatgttat ttctttaatg ccaaagctaa attgtattca atctgaattt
     4381 tagtgtcaca tgatccttca gaaaatatta acacattgct aaaaatgtat attattatca
     4441 atttggattt tttttttttt ttgtatatta acctatgcta ttggttacta tacaaaagta
     4501 ttactgtgaa tatatgcata tacagaaccg tatcaagcgc ggggggtggt tgggttatgt
     4561 ccccatttgg aaagacctgg aaacattcat aatgactaaa attgcacgga aaaatataat
     4621 atctatgagt attgctgatc agcagcacaa aaccagaact ccatattaat aaacagccaa
     4681 tgatgcagtg agtttgtgca tgttttcatt caaactcgtg tgattgtgct caaacacctg
     4741 ttgtgagcag cagttgcaaa cagtaaacct caagaggagc cagtcaacct tcacaaacac
     4801 tcaacctctg gctcactcac taaacaattc gcgttaatat ctaaaataaa aatttccagc
     4861 accaaacagc tctcagattt cggagtacac tgaccaagag gtaagaagta acgttacatt
     4921 actaggacga ctattgtaag actagatatg taatatttgg aagcgtacgt gttttaagtg
     4981 acatatagag tagaggaact gatggaaata cgatttgcat aatgtaaaat gatatatatt
     5041 aaaatatagg ctacagtagg taacgtggtt tacatataac gttactgtgt taatgtagtg
     5101 ctcagttact cgtgttgaca gttattatta gtgcttgcta atttgtttgt taatgcttct
     5161 cttgcgtatt tgattacctt caggcgaatc taaaacatat cagatgtaga aacggcagaa
     5221 aaatgttgaa gaaaatacga gaatagtagc ttttggagca catagaaaag tacatcagat
     5281 aaagcctacc gtttaaacgc agacgccgct ctcgcactga atgctgggta ctgtagtcct
     5341 tatattgtat gcttaatgtg cgtctcaacg ggaaactaga gttaggaaaa ctacaatttc
     5401 ctgtggaaaa tgtttctgtt ttcggggaaa cagcgaaaga aagatatggt tccgcgtttt
     5461 tttcttactt tgaataataa aataagacct aactacacac ttcgcttact cttatgctaa
     5521 tttgaagcac tagcattctt tgatacaatg ataatcattt aaacatcaca gttatactac
     5581 aaatactatg gttaaactat gtctagtgta gtgtaacact atggttgcca ttgttatctt
     5641 taagtttaag tattatctgt ggtaaagcca tggttggttt ttataagagg taataaaatt
     5701 aacattgtac aattaaatga ctataaatta atgttttttt ttattttttt tacacagcta
     5761 ccttcaaaaa gggatcccgc cgccaccatg agcgcacgcg gtgagggcgc ggggcagccg
     5821 tccacttcag cccagggaca acctgccgcc ccagcgcctc agaagagagg acgcggccgc
     5881 cccaggaagc agcagcaaga accaaccggt gagccctctc ctaagagacc caggggaaga
     5941 cccaaaggca gcaaaaacaa gagtccctct aaagcagctc aaaagaaagc agaagccact
     6001 ggagaaaaac ggccaagagg cagacctagg aaatggccac aacaagttgt tcagaagaag
     6061 cctgctcagg aggaaactga agagacatcc tcacaagagt ctgccgaaga ggactagatc
     6121 gatgatgatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     6181 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     6241 ataagctgca ataaacaagt taacaacaac aattcatgtc acatctatta ccacaatgca
     6301 cagcaccttg acctggaaat tagggaaatt ataacagtca atcagtggaa gaaaatggag
     6361 gaagtatgtg attcatcagc agctgcgagc agcacagtcc aaaatcagcc acaggatcaa
     6421 gagcacccgt ggccgtatct tcgcagatct aaagcttaaa tcagttgtgt taaataagag
     6481 acattcaaaa taaatgtaaa tgagctctcc aaatcagcag acttaacatt ctttaaaatg
     6541 attgattcaa tagtgataaa aatcaggcat agccagttgt aactttagat aaattacaga
     6601 aaatgtcaaa tacagagaac cgattctttt ttatgataca tccaagcaca catttaacac
     6661 aatccaggca aaccccgaat ttcacagtca caagcactgt ttgtacaaga gctttgccta
     6721 aggacacaca gtctctataa gtccaggtcg ttggtttcac tcttatttta aacatgtgac
     6781 atttttcctg ccatcctgtc ttaggctgct gtttgcttca ttccatgtca cattaaattc
     6841 ctcagtagca ccttttacac acacagccaa tcttttccag aaaattcaat tgctttgaag
     6901 agataatgtg tgaacaaatc catttagaaa aggaaaatta agaatttgta aaatcatctg
     6961 taaattgttg gcattcttct gtatatgaac atcacatcat ttacaggtaa aggtctggtc
     7021 attaattata tgacaattta ctggtattat tttgtgaaag gggctatttt caatgcgttc
     7081 atccatcctt ttcatccctc aaatctctca ttcacgtccc cctccccatc tgcacacttt
     7141 atctcatttc caccctgctg gaatctgagc acttgtgcag ttatcagggc tcctgtattt
     7201 aggaggctct gggtgtccat gtaggggacg aacagaaaca ctgcagacct ttatagaaga
     7261 acaattgata agagtcctca tacataaaga ctccattagt aagccagtga cccaggagcc
     7321 cagaccaaca gcaaagcaga cagtgaccat ggtgagcaag ggcgaagagc tgttcaccgg
     7381 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     7441 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     7501 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     7561 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     7621 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     7681 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     7741 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     7801 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     7861 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     7921 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     7981 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     8041 tctcggcatg gacgagctgt acaagtaaag cggcccctct cgagcctcta gaactatagt
     8101 gagtcgtatt acgtagatcc agacatgata agatacattg atgagtttgg acaaaccaca
     8161 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     8221 gtaaccatta taagctgcaa taaacaagag atctgatcta gaggatcata atcagccata
     8281 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     8341 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     8401 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8461 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcatcatc gaattgcatt
     8521 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     8581 tacaaatgtg gtatggctga ttatgatcct ctagatcaga tccactagtt ctagagcggc
     8641 cattaccctg ttatccctag gccgccaccg cggtggagct ccaattcgcc ctatagtgag
     8701 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     8761 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     8821 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggaaattg
     8881 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     8941 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     9001 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     9061 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     9121 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     9181 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     9241 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     9301 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     9361 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     9421 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     9481 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     9541 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     9601 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9661 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     9721 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9781 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9841 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9901 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9961 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    10021 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    10081 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    10141 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    10201 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    10261 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    10321 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    10381 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    10441 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    10501 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    10561 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    10621 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    10681 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    10741 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    10801 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10861 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10921 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10981 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    11041 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    11101 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    11161 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    11221 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    11281 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    11341 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    11401 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    11461 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    11521 gggaacaaaa gctgggtacc gggccccccc tcgaattacc ctgttatccc tatcgagcaa
    11581 aatcaatcct aatgtaaaat agtatacgtt catcattata atgcaaacac tgtagaactg
    11641 ttgtgttgtg ccttgcattt cattctcagt ttcttatcgt ctgtctcttt ttacgacaga
    11701 gctagaaaaa actagccttc atttgccatc gccgtatgca aatccactcc agaactcaac
    11761 aggtttgttc tgcggcgggt gtaagtgtga acaccggcat gtaagtgcgc ttgagcagga
    11821 gaataaagcg ctcagagaca ccggaggaat cctttatttt gcgttgcgcc tcaggggatt
    11881 cgctgagcct tctcttcatg gccctgctct tcagacacag gtgctccgct gtttgtgttt
    11941 t
//
