LOCUS       sequence-446276-       10209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer vector with EGFP-tagged paxillin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska 
            J
  TITLE     Dynamic regulation of integrin beta1 phosphorylation supports 
            invasion of breast cancer cells.
  JOURNAL   Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
  PUBMED    40419795
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4."
COMMENT     Sequence Label: pLenti6.3/TO/V5-DEST-Paxillin-EGFP
FEATURES             Location/Qualifiers
     source          1..10209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2247..2450
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2401..2421
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2452..2470
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2473..2491
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2532..2556
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2608..2617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4330..4339
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4336..4338,4339..4341,4342..5055)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4336..4338,4339..4341,4342..5052)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4381..4402)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4642..4661)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4989..5010
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5070..5094)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5147..5188
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5212..5800
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5265..5285)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5683..5694)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5815..6131
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5982..6117
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6179..6226
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6245..6643
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6730..6963
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7035..7156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7072..7091)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7126..7145
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7196..7331
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7351..7370)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7352..7370)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7380..7397)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7380..7396)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7389..7411)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7538..7993
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7625..7644)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7835..7856
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8019..8123
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8124..8192,8193..8984)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8342..8361)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9155..9743
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9644..9663
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9897..9914
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10031..10052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10067..10072,10073..10090,10091..10097)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10105..10121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10110..10132
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10129..10145
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10129..10145
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10164..10184
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10166..10184
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcttacat aacttacggt aaatggcccg cctggctgac
     1981 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2041 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2101 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2161 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2221 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2281 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2341 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2401 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccctatca
     2461 gtgatagaga tctccctatc agtgatagag atcgtcgact agtccagtgt ggtggaattc
     2521 tgcagatatc aacaagtttg tacaaaaaag caggctggcg ccggaaccaa ttcagtcgac
     2581 tggatccggt accgaattcg cggccgcgcc accatggacg acctcgacgc cctgctggcg
     2641 gacttggagt ctaccacctc ccacatctcc aaacggcctg tgttcttgtc ggaggagacc
     2701 ccctactcat acccaactgg aaaccacaca taccaggaga ttgccgtgcc accccccgtc
     2761 cccccacccc cgtccagcga ggccctcaat ggcacaatcc ttgacccctt agaccagtgg
     2821 cagcccagcg gctcccgatt catccaccag cagcctcagt cctcatcacc tgtgtacggc
     2881 tccagtgcca aaacttccag tgtctccaac cctcaggaca gtgttggctc tccgtgctcc
     2941 cgagtgggtg aggaggagca cgtctacagc ttccccaaca agcagaaatc agctgagcct
     3001 tcacccaccg taatgagcac gtccctgggc agcaaccttt ctgaactcga ccgcctgctg
     3061 ctggaactga acgctgtaca gcataacccg ccaggcttcc ctgcagatga ggccaactca
     3121 agccccccgc ttcctggggc cctgagcccc ctctatggtg tcccagagac taacagcccc
     3181 ttgggaggca aagctgggcc cctgacgaaa gagaagccta agcggaatgg gggccggggc
     3241 ctggaggacg tgcggcccag tgtggagagt ctcttggatg aactggagag ctccgtgccc
     3301 agccccgtcc ctgccatcac tgtgaaccag ggcgagatga gcagcccgca gcgcgtcacc
     3361 tccacccaac agcagacacg catctcggcc tcctctgcca ccagggagct ggacgagctg
     3421 atggcttcgc tgtcggattt caagttcatg gcccagggga agacagggag cagctcaccc
     3481 cctggggggc ccccgaagcc cgggagccag ctggacagca tgctggggag cctgcagtct
     3541 gacctgaaca agctgggggt cgccacagtc gccaaaggag tctgcggggc ctgcaagaag
     3601 cccatcgccg ggcaggttgt gaccgccatg gggaagacgt ggcaccccga gcacttcgtc
     3661 tgcacccact gccaggagga gatcggatcc cggaacttct tcgagcggga tggacagccc
     3721 tactgtgaaa aggactacca caacctcttc tccccgcgct gctactactg caacggcccc
     3781 atcctggata aagtggtgac agcccttgac cggacgtggc accctgaaca cttcttctgt
     3841 gcacagtgtg gagccttctt tggtcccgaa gggttccacg agaaggacgg caaggcctac
     3901 tgtcgcaagg actacttcga catgttcgca cccaagtgtg gcggctgcgc ccgggccatc
     3961 ctggagaact atatctcagc cctcaacacg ctgtggcatc ctgagtgctt tgtgtgccgg
     4021 gaatgcttca cgccattcgt gaacggcagc ttcttcgagc acgacgggca gccctactgt
     4081 gaggtgcact accacgagcg gcgcggctcg ctgtgttctg gctgccagaa gcccatcacc
     4141 ggccgctgca tcaccgccat ggccaagaag ttccaccccg agcacttcgt ctgtgccttc
     4201 tgcctcaagc agctcaacaa gggcaccttc aaggagcaga acgacaagcc ttactgtcag
     4261 aactgcttcc tcaagctctt ctgccgaatt ctgcagtcga cggtaccgcg ggcccgggat
     4321 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     4381 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     4441 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     4501 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     4561 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     4621 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     4681 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     4741 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     4801 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     4861 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     4921 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     4981 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     5041 gagctgtaca agtaactcga gatatctaga cccagctttc ttgtacaaag tggttgatat
     5101 ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc
     5161 taaccctctc ctcggtctcg attctacgcg taccggttag taatgatcga caatcaacct
     5221 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5281 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5341 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5401 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5461 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5521 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5581 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     5641 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5701 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     5761 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggcgatgg taccggtgtg
     5821 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5881 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5941 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6001 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6061 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6121 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     6181 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     6241 aaccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
     6301 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
     6361 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
     6421 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
     6481 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
     6541 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
     6601 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagcacaat tcgagctcgg
     6661 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6721 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     6781 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6841 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6901 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6961 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7021 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7081 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7141 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     7201 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7261 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7321 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     7381 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     7441 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     7501 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     7561 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7621 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7681 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7741 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7801 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7861 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7921 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     7981 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8041 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     8101 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     8161 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     8221 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     8281 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     8341 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     8401 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     8461 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     8521 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     8581 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     8641 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     8701 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     8761 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     8821 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     8881 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     8941 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     9001 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9061 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9121 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9181 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9241 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9301 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     9361 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     9421 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     9481 gttcgcgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     9541 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     9601 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     9661 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     9721 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     9781 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     9841 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     9901 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     9961 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    10021 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    10081 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    10141 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
    10201 tgcaagctt
//
