LOCUS       sequence-446282-       15475 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express FRS2 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15475)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 15475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:FRS2_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..15475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      5400..5409
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6942..7076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    7097..7231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(7533..8246,8247..8249,8250..8252))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7536..8246,8247..8249,8250..8252))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7578..7599)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7927..7946
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8186..8207
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9433..9449)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9433..9449)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(9504..9522)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9532..9549)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9532..9548)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9541..9563)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9689..10144)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9826..9847)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10038..10057
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10171..10275
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10276..10344,10345..11136)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10494..10513)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11307..11895
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11796..11815
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12049..12066
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12183..12204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12219..12224,12225..12242,12243..12249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12257..12273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12262..12284
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12281..12297
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12281..12297
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12316..12336
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12318..12336
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gtcctaatgc cccagtataa accaacaggt caasctgtgr tctgtgtggg tcctaatgcc
       61 ccagtataaa ccagcaggtc aacctgtggt ctgtgtgggt cctaatgccc cagtataaac
      121 cagcaggtca acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa
      181 cgtgtggtct gtgtgggtcc taatgccctg gtataaacca gcaggtcaac ttgtggtctg
      241 tgtgggtcct aatgccccgg tataaaccag catgtcaacc tgtggtctgt gtgggtccta
      301 atgccccagt ataaaccagc agrtcaacct gtggtctgtg tgggtcctaa tgccccagta
      361 taaaccagca gghcaacctg tggtctgtgt gggtcctaat gccccagtac aaaccwycak
      421 rkccacctst gktywgtgta acgcgtcaac tagtggtyyg tktkgkwyrt aatgccccag
      481 tataaaccag caggtctacc tgtggtctgt gtgggtccta atgccccagt ataaaccagc
      541 aggtcaacct gtggtctgtg tgggtcctaa tgccccagta taaaccagca ggtctacctg
      601 tggtctgtgt gggtcctaat gcaccagtat aaaccagcag gtcaacctgt ggtctgtgtg
      661 ggttctaatg ccccagtata aaccagcagg tcaacctgtg gtctgtgtgg gtccaaatgc
      721 cccagtacaa accagcaggt caacctgtgg tctgtgtggg tcctaatgcc ccagtataaa
      781 ccagcaggtc aacctgtggt ctgtgtgggt cctaatgccc cggtayaaac cagcatgtca
      841 acctgtggtc tgtgtgggtc ctaatgcccc ggtataaacc agcagatcaa cctgtggtct
      901 gtgtgggtcc taatgcccca gtataaacca gcagatcaac ctgtggtctg tgtgggtcct
      961 aawgycccag gnataaacca gcatgtcaac ctgtggtctg tgtgggtcct aatgccccgg
     1021 tataaaccag cagatcaacc tgtggtctgt gtgggtccta atgccccagg ataaaccagc
     1081 aggtcaacct gtggtctgtg tgggtcctaa tgccccagta taaaccagca ggtcaacctg
     1141 tggtctgtgt gggtcctaat gccccagtat attgatggag actctatact gctcggtgag
     1201 ctccatcttt cggataagat gttaaaacaa ggtcccgact ctctgtggtg gtaaaaaatt
     1261 cccaggatgt ccttcctacc ctggcatcct ggacaaattt gctcactgtc ctctgtccat
     1321 catggcctcc taaccatccc catatcataa ttggctttat cactctagct ggggccgaaa
     1381 tcgcatactt ccattttatg aggtacgcta aaatcagtat gcgagccaag taatatgtcc
     1441 gaatacttcc ggcgagattc tagagtgtgc attcgatgca cgctacgcta ttccatgatg
     1501 ccccgtgaga gaattcttga atcggagaga agcaacgcaa ctcatgcggg taggttacgt
     1561 gacgatgaca tattggcgga tgtagtacgt ccaagttcca ttcatactgc tcacattcat
     1621 attttataga atgttctttt cccacagcca agtagtaagt ttaaattcaa atacagtacc
     1681 tactaagtag tagtagacgg tttcggacgc agcccctgtc tcctctccac caatatactg
     1741 gtgtgtagtg tgcggtctgg tgcaatatgg ctgccatcgc gtcatccagg tggatgctgc
     1801 acactggtgg ttgatgagga gattttgccc aaaaaagtgt tttgagtttc cagaaaagcg
     1861 cgatataaat gtaaggaatt atcattagct tattataatg gaactttctg gataacttgt
     1921 gccagagcta ggggccatta aaactttgcc ctggggtacc tcagggagct gcaaggaagt
     1981 gctatagggc taatgaaatg tttgggggaa aaaaaaaaat tttataagta aaaataaaac
     2041 taaaataatt aataatttca ttgttgaaaa ttatagtaaa attgataaac aagataagca
     2101 gcattagtat aaaaaactaa aaacaaacgt aatttaaaat aattaaattg ttttaaataa
     2161 attatacaaa ataatgttta caacgacacg gtggtgcagt aggtagtgct gtcgccagca
     2221 aagcaagaag gtcgctggtt cgagcctcag ctggatcact tgatgtttct gtgtggagca
     2281 ttcgcgtgcg tttaacccca gattaataaa gagactaagc cgaaaagaaa ataaatgtat
     2341 gaaaatgttt acgtaaaatg taaataaatg catgattaat aaaatatatg aattttaaag
     2401 attgaggtag aattgtttta ttattcacac atttggttat ttttaatcag tgacaacttg
     2461 tgatgctctt tatattgttt attacgctac tttgaatatt cagtctcaaa ttgcatgtaa
     2521 atacgacttc aaagcagaat tagtttaatt taattgcctg taaacagtgt tgtactttga
     2581 tggtgattgt ctgataataa tatgatttca ctatttaaaa tttgaaaaaa aaaaatctga
     2641 aaaatactca ccggccactt tattaggtac acctgtctaa ctgcttgtta acgtaaattt
     2701 ctaatcagcc aatcaccagg cagcaactca gtgcatttag gcatgtagac atggtcaaga
     2761 cgatctgctg cagttcaaag cgagcatcag aatggagaag caaggtgatt taagtgactt
     2821 taaacgtgac gtgtttgttg gtgccagacg ggctggtctg agtatttcag aaactgctga
     2881 tttactgggg ttttcacaca caaacatctc tggggtttac agagaatggt ccaaaaaaag
     2941 ataatatcca gtgagcggca gttctgtggg ggcaaatgtc ttgttgatgc cagaggtcag
     3001 aggagaatgg cctgactggt tccagctgat agaaaggcaa cagtaattca aataaccact
     3061 cgttagaact gaggcatgca gaagagcacc tttgaacaca acatgtcgaa ccttgaggcg
     3121 ggtgggctac agcagctcaa gaccacatcg ggtgccactc ctgtcagcta agaacaggaa
     3181 actgaggcta caattcgcac aggctcaaca taagtagaca atagaagatt gaacattatg
     3241 ttgcctggtc agatggatac attctacctt gtatcaacag ttcaggctgg tgatggtggt
     3301 gtaatggtgt gggggatata ttcttggcac actttgggcc cattagtacc aattaaccat
     3361 cgtgtcaacg ccacagccta actgagtatt gttgctgacc atgttcattt ctttttgacc
     3421 acagtgtacc catcttctga tggctacttc ctgcaggata atgcaccatg tcataaagcg
     3481 tgaatgatct cagactggtt tcttgaacat gacaatgagt tcactgtgct cgaatgacct
     3541 ccacagtcac cagatctcaa tccaatagag cgcctttggg gtgtggtaga atggaagatt
     3601 cgcatcctgg atgtgcagcc gacaaatctg catgatgcta gcatgtcaat atggaccaaa
     3661 atctctgagg aattttccag tacctggttg aaatgcaaaa ggaagggaat ctatgctaag
     3721 gcaaaaagag gtccaaccca gtactagtgg ggtgtacata ataaagtggc cggtgagtgt
     3781 atattgttaa ggatatctag ataatccaaa taatttacag cactatttaa aaaaaaaact
     3841 ttatgtcacc aagtctgcat ttatttgatc acaattacag tcgaaaaagc aatatcggga
     3901 tacattgttt ccatttaaaa taactatatt atattttaaa tatttttgac aatgttattt
     3961 ctttaatgcc aaagctaaat tgtattcaat ctgaatttta gtgtcacatg atccttcaga
     4021 aaatattaac acattgctaa aaatgtatat tattatcaat ttggattttt tttttttttt
     4081 tgtatattaa cctatgctat tggttactat acaaaagtat tactgtgaat atatgcatat
     4141 acagaaccgt atcaagcgcg gggggtggtt gggttatgtc cccatttgga aagacctgga
     4201 aacattcata atgactaaaa ttgcacggaa aaatataata tctatgagta ttgctgatca
     4261 gcagcacaaa accagaactc catattaata aacagccaat gatgcagtga gtttgtgcat
     4321 gttttcattc aaactcgtgt gattgtgctc aaacacctgt tgtgagcagc agttgcaaac
     4381 agtaaacctc aagaggagcc agtcaacctt cacaaacact caacctctgg ctcactcact
     4441 aaacaattcg cgttaatatc taaaataaaa atttccagca ccaaacagct ctcagatttc
     4501 ggagtacact gaccaagagg taagaagtaa cgttacatta ctaggacgac tattgtaaga
     4561 ctagatatgt aatatttgga agcgtacgtg ttttaagtga catatagagt agaggaactg
     4621 atggaaatac gatttgcata atgtaaaatg atatatatta aaatataggc tacagtaggt
     4681 aacgtggttt acatataacg ttactgtgtt aatgtagtgc tcagttactc gtgttgacag
     4741 ttattattag tgcttgctaa tttgtttgtt aatgcttctc ttgcgtattt gattaccttc
     4801 aggcgaatct aaaacatatc agatgtagaa acggcagaaa aatgttgaag aaaatacgag
     4861 aatagtagct tttggagcac atagaaaagt acatcagata aagcctaccg tttaaacgca
     4921 gacgccgctc tcgcactgaa tgctgggtac tgtagtcctt atattgtatg cttaatgtgc
     4981 gtctcaacgg gaaactagag ttaggaaaac tacaatttcc tgtggaaaat gtttctgttt
     5041 tcggggaaac agcgaaagaa agatatggtt ccgcgttttt ttcttacttt gaataataaa
     5101 ataagaccta actacacact tcgcttactc ttatgctaat ttgaagcact agcattcttt
     5161 gatacaatga taatcattta aacatcacag ttatactaca aatactatgg ttaaactatg
     5221 tctagtgtag tgtaacacta tggttgccat tgttatcttt aagtttaagt attatctgtg
     5281 gtaaagccat ggttggtttt tataagaggt aataaaatta acattgtaca attaaatgac
     5341 tataaattaa tgtttttttt tatttttttt acacagctac cttcaaaaag ggatcagccg
     5401 ccaccatggg tagctgttgt agctgtccag ataaagacac tgtcccagat aaccatcgga
     5461 acaagtttaa ggtcattaat gtggatgatg atgggaatga gttaggttct ggcataatgg
     5521 aacttacaga cacagaactg attttataca cccgcaaacg tgactcagta aaatggcact
     5581 acctctgcct gcgacgctat ggctatgact cgaatctctt ttcttttgaa agtggtcgaa
     5641 ggtgtcaaac tggacaagga atctttgcct ttaagtgtgc ccgtgcagaa gaattattta
     5701 acatgttgca agagattatg caaaataata gtataaatgt ggtggaagag ccagttgtag
     5761 aaagaaataa tcatcagaca gaattggaag tccctagaac acctcgaaca cctacaactc
     5821 caggatttgc tgctcagaac ttacctaatg gatatccccg atatccctca tttggagatg
     5881 cttcatccca tccgtcaagc agacatcctt ctgtgggaag tgctcgcctg ccttcagtag
     5941 gggaagaatc tacacatcct ttgcttgtgg ctgaggaaca agtacatacc tatgtcaaca
     6001 ctacaggtgt gcaagaagag cggaaaaacc gcacaagtgt gcatgttcca ttggaggcga
     6061 gggtttctaa cgctgaaagc agcacaccaa aagaagaacc aagtagtatt gaggacaggg
     6121 accctcagat tcttcttgaa cctgaaggag tcaaatttgt tttagggcca acccctgttc
     6181 aaaagcagtt aatggaaaaa gagaaactgg agcaacttgg aagagatcaa gttagtggaa
     6241 gtggagcaaa taacacagaa tgggacactg gctatgacag tgatgaacga agagatgcac
     6301 cctctgttaa caaactggtg tatgaaaata taaatgggct atctatccct agtgcctcag
     6361 gggtcaggag aggtcgtctg acatccacca gtacctcaga tacccagaat atcaacaact
     6421 cagctcagag aagaactgca ttattaaact atgaaaatct accatctttg cctcctgttt
     6481 gggaagcccg caagctaagt agggatgaag atgacaattt aggaccaaag accccatctc
     6541 taaatggcta ccataataat ctagatccaa tgcataacta tgtaaataca gagaatgtaa
     6601 cagtgccagc aagtgctcac aaaatagaat attcaaggcg tcgggactgt acaccaacag
     6661 tctttaactt tgatatcaga cgcccaagtt tagaacacag gcagcttaat tacatacagg
     6721 ttgacttgga aggtggcagt gactctgaca accctcagac tccaaaaacg cctacaactc
     6781 cccttccaca aacccctacc aggcgcacag agctgtatgc cgtgatagac atcgagagaa
     6841 ctgctgctat gtcaaatttg cagaaagcac tgccacgaga tgatggtaca tctaggaaaa
     6901 ctagacacaa tagtactgat ctgcccatgt gaatcgatga tgatccagac atgataagat
     6961 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     7021 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     7081 acaacaattc gatgatgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     7141 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     7201 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     7261 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     7321 tatggctgat tatgatcctc tagatcagat ctcttgttta ttgcagctta taatggttac
     7381 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7441 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctacgt aatacgactc
     7501 actatagttc tagaggctcg agaggggccg ctttacttgt acagctcgtc catgccgaga
     7561 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
     7621 tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg
     7681 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg
     7741 ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag
     7801 acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
     7861 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
     7921 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct
     7981 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag
     8041 cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg
     8101 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg
     8161 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
     8221 ccggtgaaca gctcttcgcc cttgctcacc atggtcactg tctgctttgc tgttggtctg
     8281 ggctcctggg tcactggctt actaatggag tctttatgta tgaggactct tatcaattgt
     8341 tcttctataa aggtctgcag tgtttctgtt cgtcccctac atggacaccc agagcctcct
     8401 aaatacagga gccctgataa ctgcacaagt gctcagattc cagcagggtg gaaatgagat
     8461 aaagtgtgca gatggggagg gggacgtgaa tgagagattt gagggatgaa aaggatggat
     8521 gaacgcattg aaaatagccc ctttcacaaa ataataccag taaattgtca tataattaat
     8581 gaccagacct ttacctgtaa atgatgtgat gttcatatac agaagaatgc caacaattta
     8641 cagatgattt tacaaattct taattttcct tttctaaatg gatttgttca cacattatct
     8701 cttcaaagca attgaatttt ctggaaaaga ttggctgtgt gtgtaaaagg tgctactgag
     8761 gaatttaatg tgacatggaa tgaagcaaac agcagcctaa gacaggatgg caggaaaaat
     8821 gtcacatgtt taaaataaga gtgaaaccaa cgacctggac ttatagagac tgtgtgtcct
     8881 taggcaaagc tcttgtacaa acagtgcttg tgactgtgaa attcggggtt tgcctggatt
     8941 gtgttaaatg tgtgcttgga tgtatcataa aaaagaatcg gttctctgta tttgacattt
     9001 tctgtaattt atctaaagtt acaactggct atgcctgatt tttatcacta ttgaatcaat
     9061 cattttaaag aatgttaagt ctgctgattt ggagagctca tttacattta ttttgaatgt
     9121 ctcttattta acacaactga tttaagcttt agatctgcga agatacggcc acgggtgctc
     9181 ttgatcctgt ggctgatttt ggactgtgct gctcgcagct gctgatgaat cacatacttc
     9241 ctccattttc ttccactgat tgactgttat aatttcccta atttccaggt caaggtgctg
     9301 tgcattgtgg taatagatgt gacatgattg cattcatttt atgtttcagg ttcaggggga
     9361 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     9421 tcctctagat cagatccact agttctagag cggccattac cctgttatcc ctaggccgcc
     9481 accgcggtgg agctccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     9541 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     9601 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     9661 acagttgcgc agcctgaatg gcgaatggaa attgtaagcg ttaatatttt gttaaaattc
     9721 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     9781 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     9841 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     9901 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     9961 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
    10021 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
    10081 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
    10141 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
    10201 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
    10261 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
    10321 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
    10381 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
    10441 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
    10501 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
    10561 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
    10621 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
    10681 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
    10741 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
    10801 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
    10861 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
    10921 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
    10981 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
    11041 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
    11101 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
    11161 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    11221 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    11281 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    11341 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    11401 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    11461 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    11521 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    11581 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    11641 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    11701 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    11761 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    11821 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    11881 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    11941 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    12001 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    12061 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
    12121 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    12181 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    12241 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
    12301 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctggg taccgggccc
    12361 cccctcgaat taccctgtta tccctatcga gcaaaatcaa tcctaatgta aaatagtata
    12421 cgttcatcat tataatgcaa acactgtaga actgttgtgt tgtgccttgc atttcattct
    12481 cagtttctta tcgtctgtct ctttttacga cagagctaga aaaaactagc cttcatttgc
    12541 catcgccgta tgcaaatcca ctccagaact caacaggttt gttctgcggc gggtgtaagt
    12601 gtgaacaccg gcatgtaagt gcgcttgagc aggagaataa agcgctcaga gacaccggag
    12661 gaatccttta ttttgcgttg cgcctcaggg gattcgctga gccttctctt catggccctg
    12721 ctcttcagac acaggtgctc cgctgtttgt gtttttcact gttcttcagg cactcgtggt
    12781 tacgggtttt tgttttgcac tcttgcacac acatctctcg gcctccaaaa caaacacaca
    12841 actgcacgag ggctcgaagc ccccaccgaa aaacaaaaac attatgctaa tgaggaaggc
    12901 acagctgttc ccttcttcac tctctctctc gttctttcaa caacagtcgc ctaatttctc
    12961 tcgttccttt aaagagccgc caagcagaaa cagttaatgc aaatataagc ctgcgggaaa
    13021 taaggagaat ttttacaaaa tccgagcttc ccctcctgcc aaaatgtgat gtgtgactca
    13081 cgcattcgat atttggtgta atgagagtga atcattctgc tccacaccga acccgatgcc
    13141 acagaccact cagatgcaca ccgaggagac tattttaaca gcacattaca tccagactgt
    13201 cacagatgca ttaccaagag tgtgatttca gtgtcgaatg caggtcttaa aggaaaatct
    13261 gttgcgattg catgtgcgct aactagacac gaccaacgaa tgggaacaat ctgtgaaata
    13321 ttatacaatc acaggcaatt aaaagatgtt taaagttcaa tttagtttta attaatatgt
    13381 tatataaaac taatctgttt taaatgtata tttatgattt tagctttagc tagcctcttg
    13441 accagcagct gaagtgtccg gcaagtacct aaaagggaga ccacatggga aagctaggtt
    13501 gctcccggaa gaggtgttag taaaccagca ggtctacctg tggtctgtgt gggtcctaat
    13561 gccccagtat aaaccagcag gtcaacctgt ggtctgtgtg ggtcctaatg ccccagtata
    13621 aaccagcagg tctacctgtg gtctgtgtgg gtcctaatgc cccagtataa accagcaggt
    13681 caacctgtgg tctgtgtggg tcctaatgcc ccagtataaa ccagcaggtc aacctgtggt
    13741 ctgtgtgggt cctaatgccc cggtacaaac cagcatgtca acctgtggtc tgtgtgggtc
    13801 ctaatgcccc agtataaacc agcaggtcaa cctgtggtct gtgtgggtcc taatgcccca
    13861 gtataaacca gcaggtctac ctgtggtctg tgtgggtcct aatgccccag tataaaccag
    13921 caggtcaacc tgtggtctgt gtgggtccta atgccccagt ataaaccagc aggtctacct
    13981 gtggtctgtg tgggtcctaa tgccccagta taaaccaaca ggtcaacctg tgatctgtgt
    14041 gggtcctaat gccccagtat aaaccagcag gtcaacctgt ggtctgtgtg ggtcctaatg
    14101 ccccagtata aaccagcagg tcaacctgtg gtctgtgtgg gtcctaatgc cccagtataa
    14161 accagcaggt caacstgtgg tctgtgtggg tcctaatgcc ccrgtataaa ccagcaggtc
    14221 aacctgtggt ctgtgtgggt cctaatgccc cagtataaac cagcaggtca acctgtggtc
    14281 tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa cctgtggtct gtgtgggtcc
    14341 taatgcccca gtataaacca gcaggtcaac ctgtggtctg tgtgggtcct aatgccccgn
    14401 gtataaacca gcaggtcaac ctgtggtctg tgtgggtcct aatgccccna gtataaacca
    14461 gcaggtcaac ctgtggtctg tgtgggtcct aatgccccag tataaaccag caggtcaacc
    14521 tgtggtctgt gtgggtccta atgccccggt acaaaccagc atgtcaacct gtggtctgtg
    14581 tgggtcctaa tgccccangt ataaaccagc aggtcaacct gtggtctgtg tgggtcctaa
    14641 tgccccagta taaaccagca ggtcaacctg tggtctgtgt gggtcctaat gccccagtat
    14701 aaaccagcag gtcaacctgt ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg
    14761 tcwacctgtg gtctgtgtgg gtcctaatgc mccagtataa accagcaggt caacctgtgg
    14821 tctgtgtggg tcctaatgcc ccagtataaa ccagcaggtc aacctgtggt ctgtgtgggt
    14881 cctaatgccc cagtayaaac cagcaggtca acctgtggtc tgtgtgggtc ctaatgcccc
    14941 agtataaacc agcaggtcaa cstgtggtct gtgtgggtcc taatgccccr gtayaaacca
    15001 gcaggtcaac ctgtggtctg tgtgggtcct aatgccccgg tataaaccag caggtcaacc
    15061 tgtggtctgt gtgggtccta atgccccrgt ataaaccagc agatcaacct gtggtctgtg
    15121 tgggtcctaa tgcccmagta taaaccagca kgtcaacctg tggtctgtgt gggtcctaat
    15181 gccccrgtat aaaccagcat gtcaacctgt ggtctgtgtg ggtcctaatg ccccagkata
    15241 aaccagcagg tcaacctgtg gtctgtgtgg gtcctaatgc cccagtataa gtcagaatct
    15301 gaagcgttga tcggtgtgag tcctactgtc ccagtataaa ccagcagctc atcctgtggt
    15361 ctgtgtgggt cctaatgccc cagtataaac cagcatgtct acctgtggtc tgtgtgggtc
    15421 ctaatgcccc agtataaacc agcaggtcaa cctgtggtct gtgtgggtcc taatg
//
