LOCUS       sequence-446284-        8929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector with clover-tagged PTP-PEST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8929)
  AUTHORS   Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska 
            J
  TITLE     Dynamic regulation of integrin beta1 phosphorylation supports 
            invasion of breast cancer cells.
  JOURNAL   Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
  PUBMED    40419795
REFERENCE   2  (bases 1 to 8929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4."
COMMENT     Sequence Label: pEF.DEST51-PTPN12-Clover
FEATURES             Location/Qualifiers
     source          1..8929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(166..185)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        473..1651
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          704..1642
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1599..1619
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1668..1687
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1668..1686
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1718..1742
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4145..4147,4148..4150,4151..4861)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(4190..4211)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4798..4819
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4877..4901)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4927..4968
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4978..4995
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5018..5035)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5024..5248
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5294..5722
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5381..5400)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5591..5612
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5731..5751)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5736..6065
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5916..6051
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5978..5997
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6113..6160
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6179..6577
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6735..6856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6772..6791)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6826..6845
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6905..6921)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6905..6921)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6918..6940)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6929..6945
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6953..6959,6960..6977,6978..6983))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6998..7019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7136..7153)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7296..7884)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7376..7395)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8055..8846,8847..8915))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8678..8697
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8916..8929,1..91))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
     1681 tatagggaga cccaagctgg ctaggtaagc ttgatcaaca agtttgtaca aaaaagcagg
     1741 ctggcgccgg aaccaattca gtcgactgga tccggtaccg aggagatctg ccgccgcgat
     1801 cgccatggag caagtggaga tcctgaggaa attcatccag agggtccagg ccatgaagag
     1861 tcctgaccac aatggggagg acaacttcgc ccgggacttc atgcggttaa gaagattgtc
     1921 taccaaatat agaacagaaa agatatatcc cacagccact ggagaaaaag aagaaaatgt
     1981 taaaaagaac agatacaagg acatactgcc atttgatcac agccgagtta aattgacatt
     2041 aaagactcct tcacaagatt cagactatat caatgcaaat tttataaagg gcgtctatgg
     2101 gccaaaagca tatgtagcaa ctcaaggacc tttagcaaat acagtaatag atttttggag
     2161 gatgatatgg gagtataatg ttgtgatcat tgtaatggcc tgccgagaat ttgagatggg
     2221 aaggaaaaaa tgtgagcgct attggccttt gtatggagaa gaccccataa cgtttgcacc
     2281 atttaaaatt tcttgtgagg atgaacaagc aagaacagac tacttcatca ggacactctt
     2341 acttgaattt caaaatgaat ctcgtaggct gtatcagttt cattatgtga actggccaga
     2401 ccatgatgtt ccttcatcat ttgattctat tctggacatg ataagcttaa tgaggaaata
     2461 tcaagaacat gaagatgttc ctatttgtat tcattgcagt gcaggctgtg gaagaacagg
     2521 tgccatttgt gccatagatt atacgtggaa tttactaaaa gctgggaaaa taccagagga
     2581 atttaatgta tttaatttaa tacaagaaat gagaacacaa aggcattctg cagtacaaac
     2641 aaaggagcaa tatgaacttg ttcatagagc tattgcccaa ctgtttgaaa aacagctaca
     2701 actatatgaa attcatggag ctcagaaaat tgctgatgga gtgaatgaaa ttaacactga
     2761 aaacatggtc agctccatag agcctgaaaa acaagattct cctcctccaa aaccaccaag
     2821 gacccgcagt tgccttgttg aaggggatgc taaagaagaa atactgcagc caccggaacc
     2881 tcatccagtg ccacccatct tgacaccttc tcccccttca gcttttccaa cagtcactac
     2941 tgtgtggcag gacaatgata gataccatcc aaagccagtg ttgcatatgg tttcatcaga
     3001 acaacattca gcagacctca acagaaacta tagtaaatca acagaacttc cagggaaaaa
     3061 tgaatcaaca attgaacaga tagataaaaa attggaacga aatttaagtt ttgagattaa
     3121 gaaggtccct ctccaagagg gaccaaaaag ttttgatggg aacacacttt tgaatagggg
     3181 acatgcaatt aaaattaaat ctgcttcacc ttgtatagct gataaaatct ctaagccaca
     3241 ggaattaagt tcagatctaa atgtcggtga tacttcccag aattcttgtg tggactgcag
     3301 tgtaacacaa tcaaacaaag tttcagttac tccaccagaa gaatcccaga attcagacac
     3361 acctccaagg ccagaccgct tgcctcttga tgagaaagga catgtaacgt ggtcatttca
     3421 tggacctgaa aatgccatac ccatacctga tttatctgaa ggcaattcct cagatatcaa
     3481 ctatcaaact aggaaaactg tgagtttaac accaagtcct acaacacaag ttgaaacacc
     3541 tgatcttgtg gatcatgata acacttcacc actcttcaga acacccctca gttttactaa
     3601 tccacttcac tctgatgact cagactcaga tgaaagaaac tctgatggtg ctgtgaccca
     3661 gaataaaact aatatttcaa cagcaagtgc cacagtttct gctgccacta gtactgaaag
     3721 catttctact aggaaagtat tgccaatgtc cattgctaga cataatatag caggaacaac
     3781 acattcaggt gctgaaaaag atgttgatgt tagtgaagat tcacctcctc ccctacctga
     3841 aagaactcct gaatcgtttg tgttagcaag tgaacataat acacctgtaa gatcggaatg
     3901 gagtgaactt caaagtcagg aacgatctga acaaaaaaag tctgaaggct tgataacctc
     3961 tgaaaatgag aaatgtgatc atccagcggg aggtattcac tatgaaatgt gcatagaatg
     4021 tccacctact ttcagtgaca agagagaaca aatatcagaa aatccaacag aagccacaga
     4081 tattggtttt ggtaatcgat gtggaaaacc caaaggacca agagatccac cttcatgcgg
     4141 ccgcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     4201 ggacggcgac gtaaacggcc acaagttcag cgtccgcggc gagggcgagg gcgatgccac
     4261 caacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     4321 caccctcgtg accaccttcg gctacggcgt ggcctgcttc agccgctacc ccgaccacat
     4381 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     4441 ctctttcaag gacgacggta cctacaagac ccgcgccgag gtgaagttcg agggcgacac
     4501 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     4561 gcacaagctg gagtacaact tcaacagcca caacgtctat atcacggccg acaagcagaa
     4621 gaacggcatc aaggctaact tcaagatccg ccacaacgtt gaggacggca gcgtgcagct
     4681 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     4741 ccactacctg agccatcagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     4801 ggtcctgctg gagttcgtga ccgccgccgg gattacacat ggcatggacg agctgtacaa
     4861 gtagtaagat atctagaccc agctttcttg tacaaagtgg ttgatctaga gggcccgcgg
     4921 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat
     4981 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     5041 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     5101 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     5161 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     5221 catgctgggg atgcggtggg cgctatggct tctgaggcgg aaagaaccag ctggggctct
     5281 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5341 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5401 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5461 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5521 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5581 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5641 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     5701 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     5761 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5821 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     5881 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     5941 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6001 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     6061 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     6121 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     6181 ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa
     6241 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg
     6301 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt
     6361 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa
     6421 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca
     6481 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg
     6541 tgaattgctg ccctctggtt atgtgtggga gggctaagca cttcgtggcc gaggagcagg
     6601 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     6661 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     6721 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6781 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6841 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     6901 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6961 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7021 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7081 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7141 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7201 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7261 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7321 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7381 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7441 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7501 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7561 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7621 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7681 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7741 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7801 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7861 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7921 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7981 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8041 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8101 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8161 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8221 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8281 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8341 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8401 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8461 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8521 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8581 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8641 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8701 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8761 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8821 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8881 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttc
//
