LOCUS       sequence-446290-        4802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with clover-tagged Shp2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska 
            J
  TITLE     Dynamic regulation of integrin beta1 phosphorylation supports 
            invasion of breast cancer cells.
  JOURNAL   Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
  PUBMED    40419795
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4."
COMMENT     Sequence Label: pENTR2b-PTPN11-Clover
FEATURES             Location/Qualifiers
     source          1..4802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(2299..2301,2302..2304,2305..3015)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(2344..2365)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2952..2973
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3031..3130)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3242..3261)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3253..4062
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3320..3339)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4152..4740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4641..4660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctggc gccggaacca attcagtcga
      481 ctggatccgg taccgaggag atctgccgcc gcgatcgcca tgacatcgcg gagatggttt
      541 cacccaaata tcactggtgt ggaggcagaa aacctactgt tgacaagagg agttgatggc
      601 agttttttgg caaggcctag taaaagtaac cctggagact tcacactttc cgttagaaga
      661 aatggagctg tcacccacat caagattcag aacactggtg attactatga cctgtatgga
      721 ggggagaaat ttgccacttt ggctgagttg gtccagtatt acatggaaca tcacgggcaa
      781 ttaaaagaga agaatggaga tgtcattgag cttaaatatc ctctgaactg tgcagatcct
      841 acctctgaaa ggtggtttca tggacatctc tctgggaaag aagcagagaa attattaact
      901 gaaaaaggaa aacatggtag ttttcttgta cgagagagcc agagccaccc tggagatttt
      961 gttctttctg tgcgcactgg tgatgacaaa ggggagagca atgacggcaa gtctaaagtg
     1021 acccatgtta tgattcgctg tcaggaactg aaatacgacg ttggtggagg agaacggttt
     1081 gattctttga cagatcttgt ggaacattat aagaagaatc ctatggtgga aacattgggt
     1141 acagtactac aactcaagca gccccttaac acgactcgta taaatgctgc tgaaatagaa
     1201 agcagagttc gagaactaag caaattagct gagaccacag ataaagtcaa acaaggcttt
     1261 tgggaagaat ttgagacact acaacaacag gagtgcaaac ttctctacag ccgaaaagag
     1321 ggtcaaaggc aagaaaacaa aaacaaaaat agatataaaa acatcctgcc ctttgatcat
     1381 accagggttg tcctacacga tggtgatccc aatgagcctg tttcagatta catcaatgca
     1441 aatatcatca tgcctgaatt tgaaaccaag tgcaacaatt caaagcccaa aaagagttac
     1501 attgccacac aaggctgcct gcaaaacacg gtgaatgact tttggcggat ggtgttccaa
     1561 gaaaactccc gagtgattgt catgacaacg aaagaagtgg agagaggaaa gagtaaatgt
     1621 gtcaaatact ggcctgatga gtatgctcta aaagaatatg gcgtcatgcg tgttaggaac
     1681 gtcaaagaaa gcgccgctca tgactatacg ctaagagaac ttaaactttc aaaggttgga
     1741 caagggaata cggagagaac ggtctggcaa taccactttc ggacctggcc ggaccacggc
     1801 gtgcccagcg accctggggg cgtgctggac ttcctggagg aggtgcacca taagcaggag
     1861 agcatcatgg atgcagggcc ggtcgtggtg cactgcagtg ctggaattgg ccggacaggg
     1921 acgttcattg tgattgatat tcttattgac atcatcagag agaaaggtgt tgactgcgat
     1981 attgacgttc ccaaaaccat ccagatggtg cggtctcaga ggtcagggat ggtccagaca
     2041 gaagcacagt accgatttat ctatatggcg gtccagcatt atattgaaac actacagcgc
     2101 aggattgaag aagagcagaa aagcaagagg aaagggcacg aatatacaaa tattaagtat
     2161 tctctagcgg accagacgag tggagatcag agccctctcc cgccttgtac tccaacgcca
     2221 ccctgtgcag aaatgagaga agacagtgct agagtctatg aaaacgtggg cctgatgcaa
     2281 cagcagaaaa gcggccgcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2341 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtccg cggcgagggc
     2401 gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2461 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcgtggcctg cttcagccgc
     2521 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2581 caggagcgca ccatctcttt caaggacgac ggtacctaca agacccgcgc cgaggtgaag
     2641 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2701 ggcaacatcc tggggcacaa gctggagtac aacttcaaca gccacaacgt ctatatcacg
     2761 gccgacaagc agaagaacgg catcaaggct aacttcaaga tccgccacaa cgttgaggac
     2821 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2881 ctgctgcccg acaaccacta cctgagccat cagtccgccc tgagcaaaga ccccaacgag
     2941 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggattac acatggcatg
     3001 gacgagctgt acaagtagta agatatctag acccagcttt cttgtacaaa gttggcatta
     3061 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     3121 tcattatttg ccatccagct gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     3181 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     3241 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     3301 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     3361 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     3421 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     3481 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     3541 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     3601 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     3661 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     3721 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     3781 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     3841 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     3901 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     3961 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     4021 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     4081 ttgtaacatt attcagattg ggccccgttc cactgagcgt cagaccccgt agaaaagatc
     4141 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4201 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4261 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     4321 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4381 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4441 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4501 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4561 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4621 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4681 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4741 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4801 tt
//
