LOCUS       sequence-44633-e         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Naccache SN, Hasson T, Horowitz A
  TITLE     Binding of internalized receptors to the PDZ domain of GIPC/synectin
            recruits myosin VI to endocytic vesicles.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Aug 22;103(34):12735-40. Epub 2006 
            Aug 14.
  PUBMED    16908842
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2006-08-22"; volume: "103(34)"; pages: 
            "12735-40. Epub 2006 Aug 14"
COMMENT     Sequence Label: pcDNA4/HisMax-Synectin
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    104..266
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             279..296
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             300..332
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(336..344,345..359)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttagcgcag aggcttgggg
      121 cagccgagcg gcagccaggc cccggcccgg gcctcggttc cagaagggag aggagcccgc
      181 caaggcgcgc aagagagcgg gctgcctcgc agtccgagcc ggagagggag cgcgagccgc
      241 gccggccccg gacggcctcc gaaaccatgg ggggttctca tcatcatcat catcatggta
      301 tggctagcat gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg
      361 taccaggatc cctgctcatg ccactgggac tggggcgaag gaagaaagca ccgcctctgg
      421 tggaaaatga ggaggcggag ccaagccgga gtgggctggg tgttggggag cctgggcccc
      481 tgggtgggag cgcagcagga gaatcccaga tgggcttgcc cccacctcct gctgccctcc
      541 ggcctcgcct cgtgttccat acccagctgg cccacggcag ccccacaggc cgcatcgagg
      601 gcttcactaa tgtcaaggag ctgtacggca agatcgctga ggccttccga ctaccagctg
      661 ctgaggtgat gttctgcacc ctcaacaccc acaaagtgga catggacaga ct
//
