LOCUS       sequence-446354-       15829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CYP27B1 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15829)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 15829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:CYP27B1_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..15829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    9432..9566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    9587..9721
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(10023..10736,10737..10739,10740..10742))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(10026..10736,10737..10739,10740..10742))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10068..10089)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     10417..10436
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10676..10697
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11923..11939)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11923..11939)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(11994..12012)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12022..12039)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12022..12038)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12031..12053)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(12179..12634)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12316..12337)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12528..12547
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        12661..12765
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12766..12834,12835..13626)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12984..13003)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13797..14385
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14286..14305
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14539..14556
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14673..14694
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14709..14714,14715..14732,14733..14739)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14747..14763
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14752..14774
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14771..14787
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14771..14787
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14806..14826
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14808..14826
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 taaaagatgt ttaaagttca atttagtttt aattaatatg ttatataaaa ctaatctgtt
       61 ttaaatgtat atttatgatt ttagctttag ctagcctctt gaccagcagc tgaagtgtcc
      121 ggcaagtacc taaaagggag accacatggg aaagctaggt tgctcccgga agaggtgtta
      181 gtaaaccagc aggtctacct gtggtctgtg tgggtcctaa tgccccagta taaaccagca
      241 ggtcaacctg tggtctgtgt gggtcctaat gccccagtat aaaccagcag gtctacctgt
      301 ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg tcaacctgta gtctgtgtgg
      361 gtcctaatgc cccagtataa accagcaggt ctacctgtgg tctgtgtggg tcctaatgcc
      421 ccagtataaa ccaacaggtc aacctgtgrt ctgtgtgggt cctaatgccc cagtataaac
      481 cagcaggtca acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa
      541 cctgtggtct gtgtgggtcc taatgcccca gtataaacca gcaggtcaac gtgtggtctg
      601 tgtgggtcct aatgccctgg tataaaccag caggtcaacy tgtggtctgt gtgggtccta
      661 atgccccggt ataaaccagc atgtcaacct gtggtctgtg tgggtcctaa tgccccrgta
      721 taaaccagca grtcaacctg tggtctgtgt gggtcctaat gccccagtat aaaccagcag
      781 gtcaacctgt ggtctgtgtg ggtcctaatg ccccagtata aaccagcakg tcaacctgtg
      841 gtctgtgtgg gtcctaatgc cccagtataa accagcaggt caacctgtgg tctgtgtggg
      901 tcctaatgcc ccagtataaa ccagcaggtc aacctgtggt ctgtgtgggt cctaatgccc
      961 yrgtataaac cagcaggtca acytgtggtc tgtgtgggtc ctaatgcccc rgtataaacc
     1021 agcakgtcaa cctgtggtct gtgtgggtcc taatgcccca ngtataaacc agcaggtcaa
     1081 cctgtggtct gtgtgggtcc taatgcccca gtataaacca gcaggtcaac ctgtggtctg
     1141 tgtgggtcct aatgccccgg tayaaaccag catgtcaacc tgtggtctgt gtgggtccta
     1201 atgccccagt ataaaccagc aggtcaacct gtggtctgtg tgggtcctaa tgccccagta
     1261 taaaccagca ggtcwacctg tggtctgtgt gggtcctaat gccccagtat aaaccagcag
     1321 gtcaacctgt ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg tctacctgtg
     1381 gtctgtgtgg gtcctaatgc ncncagtata aaccagcagg tcaacctgtg gtctgtgtgg
     1441 gtyctaatgc cccagtataa accagcaggt caacctgtgg tctgtgtggg tccwaatgcc
     1501 ccagtayaaa ccagcaggtc aacctgtggt ctgtgtgggt cctaatgccc cagtataaac
     1561 cagcaggtca acctgtggtc tgtgtgggtc ctaatgcccc nggtayaaac cagcatgtca
     1621 acctgtggtc tgtgtgggtc ctaatgcccc ggtataaacc agcagrtcaa cctgtggtct
     1681 gtgtgggtcc taatgcccca gtataaacca gcaggtcaac ctgtggtctg tgtgggtcct
     1741 aatgccccag gtataaacca gcatgtcaac ctgtggtctg tgtgggtcct aatgccccrg
     1801 tataaaccag cagrtcaacc tgtggtctgt gtgggtccta atgccccagt ataaaccagc
     1861 aggtcaacct gtggtctgtg tgggtcctaa tgccccggta yaaaccagca tgtcaacctg
     1921 tggtctgtgt gggtcctaat gccccagtat aaaccagcag gtcaacctgt ggtctgtgtg
     1981 ggtcctaatg ccccagtata aaccagcagg tcwacctgtg gtctgtgtgg gtcctaatgc
     2041 cccrgtataa accagcakgt caacctgtgg tctgtgtggg tcctaatgcc ccagtataaa
     2101 ccagcaggtc wacctgtggt ctgtgtgggt cctaatgccc cagtataaac cagcaggtca
     2161 acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtctn acctgtggtc
     2221 tgtgtgggtc ctaatgcacc agtataaacc agcaggtcaa cctgtgrtct gtgtgggtyc
     2281 taatgcccca gtataaacca gcaggtcaac ctgtggtctg tgtgggtcct ratkccccag
     2341 yayattgnag agcccacgcg acacgctgcc atctcgtatg ccgccttctg cttgcaacaa
     2401 actacggacg aacttgatct gtacaaggaa gtgtgcgtgg ctgtgataat gatactccgg
     2461 ggagggaggg ctacgcctac atgatcgtcg gtagcgtcag atgtgtatga gaggcaggtg
     2521 tagatcctta tgctcgagta caaaccagca ggtgtacctg tggtctgtgt gggtcctaat
     2581 gctccagtat aaaccagcag gtcaacctgt ggtctgtgtg ggttctaatg ccccagtata
     2641 aaccagcagg tcaacctgtg gtctgtgtgg gtcgtataaa ccagcaggtc aacctgtggt
     2701 ctgtgtgggt cctaatgccc cagtataaac cagcaggtca acctgtggtc tgtgtgggtc
     2761 ctaatgcccc agtataaacc agcaggtcaa cctgtggtct gtgtgggtcg taatgcccca
     2821 gtataaacca gcaggtcaac ctgtggtctg tgtgggttgt agcttagaca cataaacgag
     2881 ccgaggaaac acggggcaat ctcgtatggc gcctgctgct tgaacctgtg gtctgtgtgg
     2941 gtcctaatgc cccagtataa accagcaggt caacctgtgg tctgtgtggg tcctaatgcc
     3001 ccagtataaa ccagcaggtc aacctgtggt ctgtgtgggt cmtaatgccc cagtataaac
     3061 cagcaggtca acctgtggtc nntgtgtggg tcctaatgcc ccagtataaa ccagcaggtc
     3121 aacctgtggt ctgtgtgggt cctaatgccc cagtataaac cagcaggtca acctgtggtc
     3181 tgtgtgggtc cwaatgcccc agtayaaacc agcaggtcaa cctgtggtct gtgtgggtcc
     3241 taatgcccca gtataaacca gcaggtcaac ctgtggtctg tgtgggtcct aatgccccrg
     3301 tataaaccag cakgtcaacc tgtggtctgt gtgggtccta atgccccrgt ataaaccagc
     3361 agrtcaacct gtggtctgtg tgggtcctaa tgccccagta taaaccagca gatcaacctg
     3421 tggtctgtgt gggtcctaaa ngntcccagg nataaaccag catgtcaacc tgtggtctgt
     3481 gtgggtccta atgccccggt ataaaccagc agatcaacct gtggtctgtg tgggtcctaa
     3541 tgccccagga taaaccagca ggtcaacctg tggtctgtgt gggtcctaat gccccagtat
     3601 aaaccagcag gtcaacctgt ggtctgtgtg ggtcctaatg ccccagtata ttgatggaga
     3661 ctctatactg ctcggtgagc tccatctttc ggataagatg ttaaaacaag gtcccgactc
     3721 tctgtggtgg taaaaaattc ccaggatgtc cttcctaccc tggcatcctg gacaaatttg
     3781 ctcactgtcc tctgtccatc atggcctcct aaccatcccc atatcataat tggctttatc
     3841 actctagctg gggccgaaat cgcatacttc cattttatga ggtacgctaa aatcagtatg
     3901 cgagccaagt aatatgtccg aatacttccg gcgagattct agagtgtgca ttcgatgcac
     3961 gctacgctat tccatgatgc cccgtgagag aattcttgaa tcggagagaa gcaacgcaac
     4021 tcatgcgggt aggttacgtg acgatgacat attggcggat gtagtacgtc caagttccat
     4081 tcatactgct cacattcata ttttatagaa tgttcttttc ccacagccaa gtagtaagtt
     4141 taaattcaaa tacagtacct actaagtagt agtagacggt ttcggacgca gcccctgtct
     4201 cctctccacc aatatactgg tgtgtagtgt gcggtctggt gcaatatggc tgccatcgcg
     4261 tcatccaggt ggatgctgca cactggtggt tgatgaggag attttgccca aaaaagtgtt
     4321 ttgagtttcc agaaaagcgc gatataaatg taaggaatta tcattagctt attataatgg
     4381 aactttctgg ataacttgtg ccagagctag gggccattaa aactttgccc tggggtacct
     4441 cagggagctg caaggaagtg ctatagggct aatgaaatgt ttgggggaaa aaaaaaaatt
     4501 ttataagtaa aaataaaact aaaataatta ataatttcat tgttgaaaat tatagtaaaa
     4561 ttgataaaca agataagcag cattagtata aaaaactaaa aacaaacgta atttaaaata
     4621 attaaattgt tttaaataaa ttatacaaaa taatgtttac aacgacacgg tggtgcagta
     4681 ggtagtgctg tcgccagcaa agcaagaagg tcgctggttc gagcctcagc tggatcactt
     4741 gatgtttctg tgtggagcat tcgcgtgcgt ttaaccccag attaataaag agactaagcc
     4801 gaaaagaaaa taaatgtatg aaaatgttta cgtaaaatgt aaataaatgc atgattaata
     4861 aaatatatga attttaaaga ttgaggtaga attgttttat tattcacaca tttggttatt
     4921 tttaatcagt gacaacttgt gatgctcttt atattgttta ttacgctact ttgaatattc
     4981 agtctcaaat tgcatgtaaa tacgacttca aagcagaatt agtttaattt aattgcctgt
     5041 aaacagtgtt gtactttgat ggtgattgtc tgataataat atgatttcac tatttaaaat
     5101 ttgaaaaaaa aaaatctgaa aaatactcac cggccacttt attaggtaca cctgtctaac
     5161 tgcttgttaa cgtaaatttc taatcagcca atcaccaggc agcaactcag tgcatttagg
     5221 catgtagaca tggtcaagac gatctgctgc agttcaaagc gagcatcaga atggagaagc
     5281 aaggtgattt aagtgacttt aaacgtgacg tgtttgttgg tgccagacgg gctggtctga
     5341 gtatttcaga aactgctgat ttactggggt tttcacacac aaacatctct ggggtttaca
     5401 gagaatggtc caaaaaaaga taatatccag tgagcggcag ttctgtgggg gcaaatgtct
     5461 tgttgatgcc agaggtcaga ggagaatggc ctgactggtt ccagctgata gaaaggcaac
     5521 agtaattcaa ataaccactc gttagaactg aggcatgcag aagagcacct ttgaacacaa
     5581 catgtcgaac cttgaggcgg gtgggctaca gcagctcaag accacatcgg gtgccactcc
     5641 tgtcagctaa gaacaggaaa ctgaggctac aattcgcaca ggctcaacat aagtagacaa
     5701 tagaagattg aacattatgt tgcctggtca gatggataca ttctaccttg tatcaacagt
     5761 tcaggctggt gatggtggtg taatggtgtg ggggatatat tcttggcaca ctttgggccc
     5821 attagtacca attaaccatc gtgtcaacgc cacagcctaa ctgagtattg ttgctgacca
     5881 tgttcatttc tttttgacca cagtgtaccc atcttctgat ggctacttcc tgcaggataa
     5941 tgcaccatgt cataaagcgt gaatgatctc agactggttt cttgaacatg acaatgagtt
     6001 cactgtgctc gaatgacctc cacagtcacc agatctcaat ccaatagagc gcctttgggg
     6061 tgtggtagaa tggaagattc gcatcctgga tgtgcagccg acaaatctgc atgatgctag
     6121 catgtcaata tggaccaaaa tctctgagga attttccagt acctggttga aatgcaaaag
     6181 gaagggaatc tatgctaagg caaaaagagg tccaacccag tactagtggg gtgtacataa
     6241 taaagtggcc ggtgagtgta tattgttaag gatatctaga taatccaaat aatttacagc
     6301 actatttaaa aaaaaaactt tatgtcacca agtctgcatt tatttgatca caattacagt
     6361 cgaaaaagca atatcgggat acattgtttc catttaaaat aactatatta tattttaaat
     6421 atttttgaca atgttatttc tttaatgcca aagctaaatt gtattcaatc tgaattttag
     6481 tgtcacatga tccttcagaa aatattaaca cattgctaaa aatgtatatt attatcaatt
     6541 tggatttttt tttttttttt gtatattaac ctatgctatt ggttactata caaaagtatt
     6601 actgtgaata tatgcatata cagaaccgta tcaagcgcgg ggggtggttg ggttatgtcc
     6661 ccatttggaa agacctggaa acattcataa tgactaaaat tgcacggaaa aatataatat
     6721 ctatgagtat tgctgatcag cagcacaaaa ccagaactcc atattaataa acagccaatg
     6781 atgcagtgag tttgtgcatg ttttcattca aactcgtgtg attgtgctca aacacctgtt
     6841 gtgagcagca gttgcaaaca gtaaacctca agaggagcca gtcaaccttc acaaacactc
     6901 aacctctggc tcactcacta aacaattcgc gttaatatct aaaataaaaa tttccagcac
     6961 caaacagctc tcagatttcg gagtacactg accaagaggt aagaagtaac gttacattac
     7021 taggacgact attgtaagac tagatatgta atatttggaa gcgtacgtgt tttaagtgac
     7081 atatagagta gaggaactga tggaaatacg atttgcataa tgtaaaatga tatatattaa
     7141 aatataggct acagtaggta acgtggttta catataacgt tactgtgtta atgtagtgct
     7201 cagttactcg tgttgacagt tattattagt gcttgctaat ttgtttgtta atgcttctct
     7261 tgcgtatttg attaccttca ggcgaatcta aaacatatca gatgtagaaa cggcagaaaa
     7321 atgttgaaga aaatacgaga atagtagctt ttggagcaca tagaaaagta catcagataa
     7381 agcctaccgt ttaaacgcag acgccgctct cgcactgaat gctgggtact gtagtcctta
     7441 tattgtatgc ttaatgtgcg tctcaacggg aaactagagt taggaaaact acaatttcct
     7501 gtggaaaatg tttctgtttt cggggaaaca gcgaaagaaa gatatggttc cgcgtttttt
     7561 tcttactttg aataataaaa taagacctaa ctacacactt cgcttactct tatgctaatt
     7621 tgaagcacta gcattctttg atacaatgat aatcatttaa acatcacagt tatactacaa
     7681 atactatggt taaactatgt ctagtgtagt gtaacactat ggttgccatt gttatcttta
     7741 agtttaagta ttatctgtgg taaagccatg gttggttttt ataagaggta ataaaattaa
     7801 cattgtacaa ttaaatgact ataaattaat gttttttttt atttttttta cacagctacc
     7861 ttcaaaaagg gatccatgac ccagaccctc aagtacgcct ccagagtgtt ccatcgcgtc
     7921 cgctgggcgc ccgagttggg cgcctcccta ggctaccgag agtaccactc agcacgccgg
     7981 agcttggcag acatcccagg cccctctacg cccagctttc tggccgaact tttctgcaag
     8041 ggggggctgt cgaggctaca cgagctgcag gtgcagggcg ccgcgcactt cgggccggtg
     8101 tggctagcca gctttgggac agtgcgcacc gtgtacgtgg ctgcccctgc actcgtcgag
     8161 gagctgctgc gacaggaggg accccggccc gagcgctgca gcttctcgcc ctggacggag
     8221 caccgccgct gccgccagcg ggcttgcgga ctgctcactg cggaaggcga agaatggcaa
     8281 aggctccgca gtctcctggc cccgctcctc ctccggcctc aagcggccgc ccgctacgcc
     8341 ggaaccctga acaacgtagt ctgcgacctt gtgcggcgtc tgaggcgcca gcggggacgt
     8401 ggcacggggc cgcccgccct ggttcgggac gtggcggggg aattttacaa gttcggactg
     8461 gaaggcatcg ccgcggttct gctcggctcg cgcttgggct gcctggaggc tcaagtgcca
     8521 cccgacacgg agaccttcat ccgcgctgtg ggctcggtgt ttgtgtccac gctgttgacc
     8581 atggcgatgc cccactggct gcgccacctt gtgcctgggc cctggggccg cctctgccga
     8641 gactgggacc agatgtttgc atttgctcag aggcacgtgg agcggcgaga ggcagaggca
     8701 gccatgagga acggaggaca gcccgagaag gacctggagt ctggggcgca cctgacccac
     8761 ttcctgttcc gggaagagtt gcctgcccag tccatcctgg gaaatgtgac agagttgcta
     8821 ttggcgggag tggacacggt gtccaacacg ctctcttggg ctctgtatga gctctcccgg
     8881 caccccgaag tccagacagc actccactca gagatcacag ctgccctgag ccctggctcc
     8941 agtgcctacc cctcagccac tgttctgtcc cagctgcccc tgctgaaggc ggtggtcaag
     9001 gaagtgctaa gactgtaccc tgtggtacct ggaaattctc gtgtcccaga caaagacatt
     9061 catgtgggtg actatattat ccccaaaaat acgctggtca ctctgtgtca ctatgccact
     9121 tcaagggacc ctgcccagtt cccagagcca aattcttttc gtccagctcg ctggctgggg
     9181 gagggtccca ccccccaccc atttgcatct cttccctttg gctttggcaa gcgcagctgt
     9241 atggggagac gcctggcaga gcttgaattg caaatggctt tggcccagat cctaacacat
     9301 tttgaggtgc agcctgagcc aggtgcggcc ccagttagac ccaagacccg gactgtcctg
     9361 gtacctgaaa ggagcatcaa cctacagttt ttggacagat agtagctaag atataggcta
     9421 ggatcgatga tgatccagac atgataagat acattgatga gtttggacaa accacaacta
     9481 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     9541 ccattataag ctgcaataaa caagttaaca acaacaattc gatgatgatc cagacatgat
     9601 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     9661 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     9721 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     9781 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc tagatcagat
     9841 ctcttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     9901 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9961 tatcatgtct ggatctacgt aatacgactc actatagttc tagaggctcg agaggggccg
    10021 ctttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
    10081 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg
    10141 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
    10201 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg
    10261 ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc
    10321 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
    10381 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
    10441 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
    10501 tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg
    10561 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg
    10621 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
    10681 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcttcgcc cttgctcacc
    10741 atggtcactg tctgctttgc tgttggtctg ggctcctggg tcactggctt actaatggag
    10801 tctttatgta tgaggactct tatcaattgt tcttctataa aggtctgcag tgtttctgtt
    10861 cgtcccctac atggacaccc agagcctcct aaatacagga gccctgataa ctgcacaagt
    10921 gctcagattc cagcagggtg gaaatgagat aaagtgtgca gatggggagg gggacgtgaa
    10981 tgagagattt gagggatgaa aaggatggat gaacgcattg aaaatagccc ctttcacaaa
    11041 ataataccag taaattgtca tataattaat gaccagacct ttacctgtaa atgatgtgat
    11101 gttcatatac agaagaatgc caacaattta cagatgattt tacaaattct taattttcct
    11161 tttctaaatg gatttgttca cacattatct cttcaaagca attgaatttt ctggaaaaga
    11221 ttggctgtgt gtgtaaaagg tgctactgag gaatttaatg tgacatggaa tgaagcaaac
    11281 agcagcctaa gacaggatgg caggaaaaat gtcacatgtt taaaataaga gtgaaaccaa
    11341 cgacctggac ttatagagac tgtgtgtcct taggcaaagc tcttgtacaa acagtgcttg
    11401 tgactgtgaa attcggggtt tgcctggatt gtgttaaatg tgtgcttgga tgtatcataa
    11461 aaaagaatcg gttctctgta tttgacattt tctgtaattt atctaaagtt acaactggct
    11521 atgcctgatt tttatcacta ttgaatcaat cattttaaag aatgttaagt ctgctgattt
    11581 ggagagctca tttacattta ttttgaatgt ctcttattta acacaactga tttaagcttt
    11641 agatctgcga agatacggcc acgggtgctc ttgatcctgt ggctgatttt ggactgtgct
    11701 gctcgcagct gctgatgaat cacatacttc ctccattttc ttccactgat tgactgttat
    11761 aatttcccta atttccaggt caaggtgctg tgcattgtgg taatagatgt gacatgattg
    11821 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
    11881 cctctacaaa tgtggtatgg ctgattatga tcctctagat cagatccact agttctagag
    11941 cggccattac cctgttatcc ctaggccgcc accgcggtgg agctccaatt cgccctatag
    12001 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
    12061 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
    12121 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa
    12181 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
    12241 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
    12301 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
    12361 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
    12421 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
    12481 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
    12541 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
    12601 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
    12661 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
    12721 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
    12781 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
    12841 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
    12901 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
    12961 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
    13021 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
    13081 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
    13141 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
    13201 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
    13261 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
    13321 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
    13381 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
    13441 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
    13501 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
    13561 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
    13621 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    13681 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    13741 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    13801 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    13861 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    13921 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    13981 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    14041 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    14101 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    14161 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    14221 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    14281 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    14341 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    14401 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    14461 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    14521 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
    14581 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
    14641 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
    14701 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
    14761 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
    14821 taaagggaac aaaagctggg taccgggccc cccctcgaat taccctgtta tccctatcga
    14881 gcaaaatcaa tcctaatgta aaatagtata cgttcatcat tataatgcaa acactgtaga
    14941 actgttgtgt tgtgccttgc atttcattct cagtttctta tcgtctgtct ctttttacga
    15001 cagagctaga aaaaactagc cttcatttgc catcgccgta tgcaaatcca ctccagaact
    15061 caacaggttt gttctgcggc gggtgtaagt gtgaacaccg gcatgtaagt gcgcttgagc
    15121 aggagaataa agcgctcaga gacaccggag gaatccttta ttttgcgttg cgcctcaggg
    15181 gattcgctga gccttctctt catggccctg ctcttcagac acaggtgctc cgctgtttgt
    15241 gtttttcact gttcttcagg cactcgtggt tacgggtttt tgttttgcac tcttgcacac
    15301 acatctctcg gcctccaaaa caaacacaca actgcacgag ggctcgaagc ccccaccgaa
    15361 aaacaaaaac attatgctaa tgaggaaggc acagctgttc ccttcttcac tctctctctc
    15421 gttctttcaa caacagtcgc ctaatttctc tcgttccttt aaagagccgc caagcagaaa
    15481 cagttaatgc aaatataagc ctgcgggaaa taaggagaat ttttacaaaa tccgagcttc
    15541 ccctcctgcc aaaatgtgat gtgtgactca cgcattcgat atttggtgta atgagagtga
    15601 atcattctgc tccacaccga acccgatgcc acagaccact cagatgcaca ccgaggagac
    15661 tattttaaca gcacattaca tccagactgt cacagatgca ttaccaagag tgtgatttca
    15721 gtgtcgaatg caggtcttaa aggaaaatct gttgcgattg catgtgcgct aactagacac
    15781 gaccaacgaa tgggaacaat ctgtgaaata ttatacaatc acaggcaat
//
