LOCUS       sequence-446364-        7754 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression androgen receptor 2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Xiao H, Wang L, Yan S, Ma H, Xu Z, Wang F, Wang J, Tao W, Wang D
  TITLE     Steroid hormone-deprived sex reversal in cyp11a1 mutant XX tilapia 
            experiences an ovary-like stage at molecular level.
  JOURNAL   Commun Biol. 2024 Sep 16;7(1):1154. doi: 10.1038/s42003-024-06853-8.
  PUBMED    39284885
REFERENCE   2  (bases 1 to 7754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2024-09-16"; volume: "7(1):1154. doi"; pages: " 
            10.1038/s42003-024-06853-8"
COMMENT     Sequence Label: pcDNA3.1-ar2
FEATURES             Location/Qualifiers
     source          1..7754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             911..934
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3252..3293
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3303..3320
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3343..3360)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3349..3573
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3619..4047
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3706..3725)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3916..3937
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4056..4076)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4061..4390
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4241..4376
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4303..4322
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4457..5251
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4511..4530)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5121..5140
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5427..5548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5464..5483)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5518..5537
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5597..5613)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5597..5613)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5610..5632)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5621..5637
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5645..5651,5652..5669,5670..5675))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5690..5711
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5828..5845)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5999..6587)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6079..6098)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6758..7549,7550..7618))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7381..7400
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7619..7723)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttatg gattataaag atgatgatga taaaagccaa acgaccagag agttattttg
      961 tgccaaaatc tggccagagg ataggaaatc atacacaggc gacgtcgtga gcgctgccag
     1021 tatggcgcaa aaaactgaag aaagtcgcgt ctatttcacc aaaaccccgg ctggaagcag
     1081 tgccgcgagt ttggcagaat ccgagaatgc aaacctgtac ggacacatta ttccgctagc
     1141 ctgtgacatg gagaaacgct gctgccagac agctgctgcc cctcaggagg agttaagcgc
     1201 tgagtgccga gtgggcgaca gtcgctcctt ttctgcctgc gccacaatct cagaaacagc
     1261 cagggagctc tgcaaagctg tgtccgtgtc tctgggtctg gccatggagt ccggtgacac
     1321 gagcgagacg cacgtttctc tcccccagtg tgccgcaaat gaccaaatga gcgcagagta
     1381 tttattcggg gtggaagccg tgcctgcgaa ctgtcccgga gccccggctt ctctctccga
     1441 gtacaggtgt cccgagcgag atgagcgccc gccacacgac cacaagcaac aggtgaaaat
     1501 gctcaaaagt tcagagcccg cggcggcttt tcatcacctc agttccacgg cgacatctgt
     1561 gaacgcgcaa aacttcacgc tgtgcgaggc tgatgacata actccgaaag agatagatca
     1621 tctggacata gcgtgcgctg cctcttgccc ttacgcccca gctgcgccgg aacctttggc
     1681 acagttcggg cccgcggctg cacagagacc gtgcagagct tacaacccag tggaggaagc
     1741 gggagacgtc ggggatgccg gggagaatag gtccgtgggg tttcaggcag aacggtacgg
     1801 cgtcaaagtc aaatctgagg gaagtgcatc tgcaggggcg ttatgggtca gcaggcctaa
     1861 ttacagcttc ggcgacaagt acaactcgca gttttggggg tcgaggcagt gcatgaactt
     1921 gcagagttcg ggagcgaact ccgcgtttat atgcaatcct tatgacggga ccgtaatgcg
     1981 ccgtgaacag tggtaccctg gcgggatgct gaggacgcct tatcccaact ccaactacat
     2041 gaagggtgag gtcggcgaat ggctggatgt cacttacaac gacaccaggt ttgagtccag
     2101 tagagaacac atgtttccca tggagttctt ctttccccca cagaggacat gcctcatctg
     2161 ctcggacgag gcatctggct gtcattacgg cgcactcacc tgtggcagct gcaaggtttt
     2221 cttcaaaagg gctgctgaag gcaagcagaa atacctgtgt gcaagcaaga atgactgcac
     2281 tattgataag ctaagaagaa agaactgccc ctcctgtcgc ctgaagaagt gctttgaagc
     2341 tgggatgact cttggagcac gtaagctgaa gaagattgga caacagaaaa gcccagaaga
     2401 agatcatggt gctcaggatc cagtggaggt tatcccgaac gtctctccta aaactggtgt
     2461 gcaactgaac tcccacctgg tcttcctcaa catcctggag tccattgagc cggaggttgt
     2521 gaatgcgggc cacgactgtg gccagccgga ctccgctgac accctgctca ccagcctcaa
     2581 tgaattggga gagagacaac ttgtcaaagt ggtcaaatgg gcaaaaggat tgccaggttt
     2641 cagaaatctc catgtggatg accagatgac tatcattcag cagtcatgga tgggggtgat
     2701 ggtgtttgcc ttgggatgga ggtcctataa gaatgccagt ggcaggattc tttactttgc
     2761 cccagacctt gtgttcaatg aacacagaat gcacgtttcc accatgtacg agcactgcat
     2821 acggatgagg catctctctc aggagtttga gatgttgcag atcacccagg aagagtttca
     2881 ctgcatgaag gccttgctcc tctttagcat tattcctgtt gagggtctga agagtcagaa
     2941 gtactttgac gaattgcgac tcacctacat caacgaactc gaccgcctca tcaactttca
     3001 aacgacgaca aacacatctc agaggttcta ccagctcacg cgtctcatgg actctctcca
     3061 gatgaccgta aagaagctcc atcagtttac cttcgacctt tttgtccagg ctcagtcact
     3121 gcacaccaag gtcagctttc cagagatgat tggagaaata atctcagtac atgtaccaaa
     3181 gatcttgaca ggtttggcta aaccaatctt gtttcataag tagctcgagt ctagagggcc
     3241 cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     3301 gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta
     3361 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     3421 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     3481 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     3541 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     3601 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3661 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     3721 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     3781 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     3841 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     3901 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     3961 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4021 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     4081 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4141 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4201 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     4261 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4321 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4381 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     4441 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     4501 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     4561 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4621 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4681 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4741 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4801 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4861 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4921 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4981 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     5041 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     5101 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     5161 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     5221 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc gaaatgaccg
     5281 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     5341 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     5401 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     5461 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5521 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     5581 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     5641 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     5701 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     5761 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     5821 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5881 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5941 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6001 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6061 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6121 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6181 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6241 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6301 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6361 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6421 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6481 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6541 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6601 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6661 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6721 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6781 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6841 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6901 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6961 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7021 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7081 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7141 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7201 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7261 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7321 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     7381 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7441 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7501 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7561 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7621 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7681 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7741 tgccacctga cgtc
//
