LOCUS       sequence-446369-        9551 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Rep/Cap plasmid for the production of MyoAAV 4E, a muscle-tropic AAV
            capsid in mice and macaques..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Tabebordbar M, Lagerborg KA, Stanton A, King EM, Ye S, Tellez L, 
            Krunnfusz A, Tavakoli S, Widrick JJ, Messemer KA, Troiano EC, 
            Moghadaszadeh B, Peacker BL, Leacock KA, Horwitz N, Beggs AH, Wagers
            AJ, Sabeti PC
  TITLE     Directed evolution of a family of AAV capsid variants enabling 
            potent muscle-directed gene delivery across species.
  JOURNAL   Cell. 2021 Sep 16;184(19):4919-4938.e22. doi: 
            10.1016/j.cell.2021.08.028. Epub 2021 Sep 9.
  PUBMED    34506722
REFERENCE   2  (bases 1 to 9551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.08.028"; journalName: "Cell"; date: 
            "2021-09-16- 16"; volume: "184"; issue: "19"; pages: "4919-4938.e22"
COMMENT     Sequence Label: kiCAP-AAV-MyoAAV4E
FEATURES             Location/Qualifiers
     source          1..9551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1909..1928)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1910..1928)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1938..1955)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1938..1954)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1947..1969)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2096..2551
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2183..2202)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2393..2414
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2577..2681
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2682..3491
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2749..2768)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3662..4250
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4151..4170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4404..4421
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4538..4559
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4574..4579,4580..4597,4598..4604)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4612..4628
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4617..4639
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4636..4652
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4636..4652
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4671..4691
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4673..4691
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(5032..5043)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    7760..7778
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7797..7815
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7834..7852
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7871..7889
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7908..7926
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     gap             7955..8054
                     /estimated_length=100
     polyA_signal    8125..8349
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8338..8355
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        8375..8393
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8375..8392
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(8432..8542)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
ORIGIN
        1 gtgagtgaag cagatatttg aattctgatt cagtctctcg cattgtctgc agggaaacag
       61 catcagattc atgcccacgt gacgagaaca tttgttttgg tacctgtccg cgtagttgat
      121 cgaagcttcc gcgtctgacg tcgatggctg cgcaactgac tcgcgcaccc gtttgggctc
      181 acttatatct gcgtcactgg gggcgggtct tttcttggct ccaccctttt tgacgtagaa
      241 ttcatgctcc acctcaacca cgtgatcctt tgcccaccgg aaaaagtctt tgacttcctg
      301 cttggtgacc ttcccaaagt catgatccag acggcgggtg agttcaaatt tgaacatccg
      361 gtcttgcaac ggctgctggt gttcgaaggt cgttgagttc ccgtcaatca cggcgcacat
      421 gttggtgttg gaggtgacga tcacgggagt cgggtctatc tgggccgagg acttgcattt
      481 ctggtccacg cgcaccttgc ttcctccgag aatggctttg gccgactcca cgaccttggc
      541 ggtcatcttc ccctcctccc accagatcac catcttgtcg acacagtcgt tgaagggaaa
      601 gttctcattg gtccagttta cgcacccgta gaagggcaca gtgtgggcta tggcctccgc
      661 gatgttggtc ttcccggtag ttgcaggccc aaacagccag atggtgttcc tcttgccgaa
      721 ctttttcgtg gcccatccca gaaagacgga agccgcatat tggggatcgt acccgtttag
      781 ttccaaaatt ttataaatcc gattgctgga aatgtcctcc acgggctgct ggcccaccag
      841 gtagtcgggg gcggttttag tcaggctcat aatctttccc gcattgtcca aggcagcctt
      901 gatttgggac cgcgagttgg aggccgcatt gaaggagatg tatgaggcct ggtcctcctg
      961 gatccactgc ttctccgagg taatcccctt gtccacgagc cacccgacca gctccatgta
     1021 cctggctgaa gtttttgatc tgatcaccgg cgcatcagaa ttgggattct gattctcttt
     1081 gttctgctcc tgcgtctgcg acacgtgcgt cagatgctgc gccaccaacc gtttacgctc
     1141 cgtgagattc aaacaggcgc ttaaatactg ttccatatta gtccacgccc actggagctc
     1201 aggctgggtt ttggggagca agtaattggg gatgtagcac tcatccacca ccttgttccc
     1261 gcctccggcg ccatttctgg tctttgtgac cgcgaaccag tttggcaaag tcggctcgat
     1321 cccgcggtaa attctctgaa tcagtttttc gcgaatctga ctcaggaaac gtcccaaaac
     1381 catggatttc accccggtgg tttccacgag cacgtgcatg tggaagtagc tctctccctt
     1441 ctcaaattgc acaaagaaaa gagcctccgg ggccttactc acacggcgcc attccgtcag
     1501 aaagtcgcgc tgcagcttct cggccacggt caggggtgcc tgctcaatca gattcagatc
     1561 catgtcagaa tctggcggca actcccattc cttctcggcc acccagttca caaagctgtc
     1621 agaaatgccg ggcagatgct cgtcaaggtc gctggggacc ttaatcacaa tctcgtaaaa
     1681 ccccggcatg gcggctgcgc gttcaaacct cccgcttcaa aatggagacc ctgcgtgctc
     1741 actcgggctt aaatacccag cgtgaccaca tggtgtcgca aaatgtcgca aaacactcac
     1801 gtgacctcta atacaggacc tccctaaccc tatgacgtaa ttcacgtcac gactccaccc
     1861 ctccacgcgt gtcgacctcg atcgaggggg ggcccggtac ccaattcgcc ctatagtgag
     1921 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     1981 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     2041 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     2101 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2161 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     2221 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     2281 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     2341 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     2401 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     2461 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     2521 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     2581 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     2641 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagccat attcaacggg
     2701 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     2761 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     2821 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     2881 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     2941 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     3001 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     3061 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     3121 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     3181 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     3241 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     3301 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     3361 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     3421 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     3481 agtttttcta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     3541 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     3601 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3661 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3721 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3781 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3841 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3901 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3961 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4021 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4081 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4141 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4201 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4261 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4321 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4381 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     4441 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     4501 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     4561 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     4621 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     4681 ctcactaaag ggaacaaaag ctggagctcc accgcggtgg cggccgctct agtcgagcgg
     4741 ccgccagtgt gatggatatc tgtagttaat gattaacccg ccatgctact tatctacgta
     4801 gccatggaaa ctagataaga aagaaatacg cagagaccaa agttcaactg aaacgaatta
     4861 aacggtttat tgattaacaa gcaattacag attacgagtc aggtatctgg tgccaatggg
     4921 gcggggttca ctatatacac cttcagtatt aacagcaaat tcaacattat tagacttgta
     4981 atagttggaa gtgtactgga tctccgggtt ccagcgcttg ctgttttcct tctgcagctc
     5041 ccactcgatc tccacgctga cttggccagt agaatactgg gtgatgaaag agttcagctt
     5101 gtccttgttg aaggccgttg gaggatccgc aggtacaggt gtgtttttga tgaggatctg
     5161 aggaggcggg tgcttcattc caaaccctcc catcagcgga gaagggtgaa agttgccgtc
     5221 cgtgtgagga attttggccc aaatgggtcc ttgcaggtac acatctctgt cctgccaaac
     5281 catacccgga agtattcctt ggttttgaac ccagccggtc tgcgcctgtg cagtgttgtt
     5341 gaagtctcct ctcctgtttt cctggtggtt tgtggccact tgtccatagg actccgttgc
     5401 taccgggtta gtagttttaa tttcttcttc gttggttatc atgactttgt ccgcatccac
     5461 gttgtctctt ccagttcctt gtttgccaaa aattaaagat ccagacaaag gaaagaaacg
     5521 gtcctctcct tctttgtggc tggccatagc aggtccagga ttcatcaagc tattacgtcc
     5581 attgagagcc caagaagaag ctccaggcca agcaaattcg ctgttgttgt tttgagtcac
     5641 agtggttgag acacgttgtt gtcggtagct gggtccaggt atgtagtttc ttccctggac
     5701 agccatgttg ctgggtccgg ccacactgaa ttttagcgtt tgttgattct gtccagaacc
     5761 gttaatagtc tttgagagat agtacaagta ttggtcgatg agtggattca ttagtcggtc
     5821 caggctttgg ctgtgagcgt agctgctatg gaaaggtacg ttctcaaact cgtagctgaa
     5881 ctggaagttg ttacccgttc ttagcatttg cgacgggaaa tattccaggc agtaaaagga
     5941 cgaacgaccc acggcctggc ttccatcatt aagcgtcaga tacccgtact gaggaatcat
     6001 gaaaacgtcc gctgggaacg gcgggaggca gccctcgtga gccgacccga gcacgtacgg
     6061 gagctgatag tctgagtccg tgaagacctg gaccgtgctg gtaaggttat tggcgatggt
     6121 cttgactcca ttgttgtccg taacctcttt gacctgaatg ttgaagagct tgaagttgag
     6181 tcgcttaggc cggaatcccc agttgttgtt gatgagtcgc tgccagtcac gtggtgagaa
     6241 gtggcagtgg aatctgttga agtcaaaata cccccagggg gtgctgtagc cgaagtaggc
     6301 gttgtcattt gaagatcctc cagatgtgct gttggagatt tgcttgtaga ggtgattgtt
     6361 gtaggtgggc agggcccagg ttcgggtgct ggtggtgatg actctgtccc ccagccattg
     6421 ggaatcgcaa tgccaatttc ccgaggaact acccactcca tcggcacctt cgttattgtc
     6481 tgccactggt gcgccaccac ctgaagccat tgtaagagat cccacacctg agggggctgc
     6541 gggaggttct ccgattggtt gagggtctgg gactgactct gtgtcgccag tctgaccgaa
     6601 attgagtctc tttttagcgg gctgtgcacc cgatttgcca atacccgcgg aggagtccgg
     6661 ttcctgagga gactgctcta caggcctctt ctttccagga gccgtcttag ccgcttcctc
     6721 aaccagacca agaggttcaa gaagcctctt tttggcctgg aagactgctc gcccgaggtt
     6781 gcccccaaaa gacgtatctt ctttgagccg ctcctggaac tcggcgtcgg cgtggttgta
     6841 cttgaggtac gggttgtctc cggccttgag ctgctggtcg taggccttgt cgtgctcgag
     6901 ggccgccgcg tctgctgcgt tgaccggctc ccccttgtcg agtccgttgc cgggtccaag
     6961 gtatttgtaa cccggaagca caagacctcg agcgttgtct tgatgttgtt gatttgcctt
     7021 gggttgaggg gctccaggtt tcaaagccca ccactcgcga attccttcac taaggttgtc
     7081 ctcgagccaa tctggaagat aaccatcggc agccatacct ggtttaagtc atttattgtt
     7141 cagaaacaca gtcatccaag tccacattga ccaggtcgca agcagtgcaa gcgtctggca
     7201 cctttcccat gatgtgatga atgtagcaca gtttctgata cgcctttttg acgacagaaa
     7261 cgggttgaga ttctgacacg ggaaagcact ctaaacagtc tttgacaccg tgagtgaagc
     7321 agatatttga attctgattc agtctctcgc attgtctgca gggaaacagc atcagattca
     7381 tgcccacgtg acgagaacat ttgttttggt acctgtccgc gtagttgatc gaagctagcg
     7441 gtcgcagagg agcgggatca acagtcacgt ctgaactgcg aggcgtctcg ggaacaaatg
     7501 agagcctggc ccgcttctcc agacttttat agctagtatt ggtgacgtca cccagtgggc
     7561 gttttagaga tttctccgcc cctttagttc ccgccaattc cctgggaact ttaaactcgt
     7621 gagtcaccgg cacctgattg acctttgccc aagcaaaaaa gtccttgact tcctgcttag
     7681 taatcttgcc aaaatctggc gggagccgct tagtcagttc aaatttgaac agatctctct
     7741 gcttatatag ggggtacctt ctctatcact gatagggagc tcgagacgtg tcgacttctc
     7801 tatcactgat agggagacgt tctagacgta ccttctctat cactgatagg gagctcgaga
     7861 cgtgtcgact tctctatcac tgatagggag acgttctaga cgtaccttct ctatcactga
     7921 tagggagctc gagacgtgtc gacttctcta tcacnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnntgatag ggagacgtca ggtggcactt gatagcttga ttcgagcccc
     8101 agctggttct ttccgcctca gaagccatag agcccaccgc atccccagca tgcctgctat
     8161 tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac
     8221 acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg
     8281 caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag
     8341 aaggcacagt cgaggctgat cagcgagctc tagcatttag gtgacactat agaatagggc
     8401 cctctagatc gaattcatgc atttacccgg ggagcatgtc aaggtcaaaa tcgtcaagag
     8461 cgtcagcagg cagcatatca aggtcaaagt cgtcaagggc atcggctggg agcatgtcta
     8521 agtcaaaatc gtcaagggcg tcggccggcc cgccgctttc gcactttagc tgtttctcca
     8581 ggccacatat gattagttcc aggccgaaaa ggaaggcagg ttcggctccc tgccggtcga
     8641 acagctcaat tgcttgtctc agaagtgggg gcatagaatc ggtggtaggt gtctctcttt
     8701 cctcttttgc tacttgatgc tcctgttcct ccaatacgca gcccagtgta aagtggccca
     8761 cggcggacag agcgtacagt gcgttctcca gggagaagcc ttgctgacac aggaacgcga
     8821 gctgattttc cagggtttcg tactgtttct ctgttgggcg ggtgccgaga tgcactttag
     8881 ccccgtcgcg atgtgagagg agagcacagc ggaatgactt ggcgttgttc cgcagaaagt
     8941 cttgccatga ctcgccttcc agggggcaga agtgggtatg atgcctgtcc agcatctcga
     9001 tggccagggc atcgagcagg gcccgcttgt tcttcacgtg ccagtacagg gtaggctgct
     9061 caactcccag cttttgagcg agtttccttg tcgtcaggcc ttcgataccg acttcattga
     9121 gtaattccag agcgccgttt atgactttag atttatccag ccgactcatg gtggcactag
     9181 ttcactaagg ttgtcctcga gccaatctgg aagataacca tcggcagcaa tacctggttt
     9241 aagtcattta ttgttcagaa acacagtcat ccaagtccac attgaccagg tcgcaagcag
     9301 tgcaagcgtc tggcaccttt cccatgatgt gatgaatgta gcacagtttc tgatacgcct
     9361 ttttgacgac agaaacgggt tgagattctg acacgggaaa gcactctaaa cagtctttga
     9421 caccgtgagt gaagcagata tttgaattct gattcagtct ctcgcattgt ctgcagggaa
     9481 acagcatcag attcatgccc acgtgacgag aacatttgtt ttggtacctg tccgcgtagt
     9541 tgatcgaagc t
//
