LOCUS       sequence-446371-        9746 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Rep/Cap plasmid for the production of MyoAAV 3F, a muscle-tropic AAV
            capsid in mice and macaques..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9746)
  AUTHORS   Tabebordbar M, Lagerborg KA, Stanton A, King EM, Ye S, Tellez L, 
            Krunnfusz A, Tavakoli S, Widrick JJ, Messemer KA, Troiano EC, 
            Moghadaszadeh B, Peacker BL, Leacock KA, Horwitz N, Beggs AH, Wagers
            AJ, Sabeti PC
  TITLE     Directed evolution of a family of AAV capsid variants enabling 
            potent muscle-directed gene delivery across species.
  JOURNAL   Cell. 2021 Sep 16;184(19):4919-4938.e22. doi: 
            10.1016/j.cell.2021.08.028. Epub 2021 Sep 9.
  PUBMED    34506722
REFERENCE   2  (bases 1 to 9746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.08.028"; journalName: "Cell"; date: 
            "2021-09-16- 16"; volume: "184"; issue: "19"; pages: "4919-4938.e22"
COMMENT     Sequence Label: kiCAP-AAV-MyoAAV3F
FEATURES             Location/Qualifiers
     source          1..9746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2104..2123)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2105..2123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2133..2150)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2133..2149)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2142..2164)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2291..2746
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2378..2397)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2588..2609
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2772..2876
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2877..3686
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2944..2963)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3857..4445
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4346..4365
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4599..4616
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4733..4754
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4769..4774,4775..4792,4793..4799)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4807..4823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4812..4834
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4831..4847
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4831..4847
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4866..4886
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4868..4886
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(5227..5238)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    7955..7973
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7992..8010
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8029..8047
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8066..8084
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8103..8121
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     gap             8150..8249
                     /estimated_length=100
     polyA_signal    8320..8544
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8533..8550
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        8570..8588
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8570..8587
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(8627..8737)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
ORIGIN
        1 atacctggtt taagtcattt attgttcaga aacacagtca tccaagtcca cattgaccag
       61 gtcgcaagca gtgcaagcgt ctggcacctt tcccatgatg tgatgaatgt agcacagttt
      121 ctgatacgcc tttttgacga cagaaacggg ttgagattct gacacgggaa agcactctaa
      181 acagtctttg acaccgtgag tgaagcagat atttgaattc tgattcagtc tctcgcattg
      241 tctgcaggga aacagcatca gattcatgcc cacgtgacga gaacatttgt tttggtacct
      301 gtccgcgtag ttgatcgaag cttccgcgtc tgacgtcgat ggctgcgcaa ctgactcgcg
      361 cacccgtttg ggctcactta tatctgcgtc actgggggcg ggtcttttct tggctccacc
      421 ctttttgacg tagaattcat gctccacctc aaccacgtga tcctttgccc accggaaaaa
      481 gtctttgact tcctgcttgg tgaccttccc aaagtcatga tccagacggc gggtgagttc
      541 aaatttgaac atccggtctt gcaacggctg ctggtgttcg aaggtcgttg agttcccgtc
      601 aatcacggcg cacatgttgg tgttggaggt gacgatcacg ggagtcgggt ctatctgggc
      661 cgaggacttg catttctggt ccacgcgcac cttgcttcct ccgagaatgg ctttggccga
      721 ctccacgacc ttggcggtca tcttcccctc ctcccaccag atcaccatct tgtcgacaca
      781 gtcgttgaag ggaaagttct cattggtcca gtttacgcac ccgtagaagg gcacagtgtg
      841 ggctatggcc tccgcgatgt tggtcttccc ggtagttgca ggcccaaaca gccagatggt
      901 gttcctcttg ccgaactttt tcgtggccca tcccagaaag acggaagccg catattgggg
      961 atcgtacccg tttagttcca aaattttata aatccgattg ctggaaatgt cctccacggg
     1021 ctgctggccc accaggtagt cgggggcggt tttagtcagg ctcataatct ttcccgcatt
     1081 gtccaaggca gccttgattt gggaccgcga gttggaggcc gcattgaagg agatgtatga
     1141 ggcctggtcc tcctggatcc actgcttctc cgaggtaatc cccttgtcca cgagccaccc
     1201 gaccagctcc atgtacctgg ctgaagtttt tgatctgatc accggcgcat cagaattggg
     1261 attctgattc tctttgttct gctcctgcgt ctgcgacacg tgcgtcagat gctgcgccac
     1321 caaccgttta cgctccgtga gattcaaaca ggcgcttaaa tactgttcca tattagtcca
     1381 cgcccactgg agctcaggct gggttttggg gagcaagtaa ttggggatgt agcactcatc
     1441 caccaccttg ttcccgcctc cggcgccatt tctggtcttt gtgaccgcga accagtttgg
     1501 caaagtcggc tcgatcccgc ggtaaattct ctgaatcagt ttttcgcgaa tctgactcag
     1561 gaaacgtccc aaaaccatgg atttcacccc ggtggtttcc acgagcacgt gcatgtggaa
     1621 gtagctctct cccttctcaa attgcacaaa gaaaagagcc tccggggcct tactcacacg
     1681 gcgccattcc gtcagaaagt cgcgctgcag cttctcggcc acggtcaggg gtgcctgctc
     1741 aatcagattc agatccatgt cagaatctgg cggcaactcc cattccttct cggccaccca
     1801 gttcacaaag ctgtcagaaa tgccgggcag atgctcgtca aggtcgctgg ggaccttaat
     1861 cacaatctcg taaaaccccg gcatggcggc tgcgcgttca aacctcccgc ttcaaaatgg
     1921 agaccctgcg tgctcactcg ggcttaaata cccagcgtga ccacatggtg tcgcaaaatg
     1981 tcgcaaaaca ctcacgtgac ctctaataca ggacctccct aaccctatga cgtaattcac
     2041 gtcacgactc cacccctcca cgcgtgtcga cctcgatcga gggggggccc ggtacccaat
     2101 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     2161 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     2221 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     2281 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     2341 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     2401 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     2461 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     2521 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     2581 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     2641 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     2701 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     2761 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     2821 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     2881 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat
     2941 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt
     3001 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     3061 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     3121 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa
     3181 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     3241 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     3301 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     3361 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     3421 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     3481 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     3541 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     3601 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     3661 atttgatgct cgatgagttt ttctaactgt cagaccaagt ttactcatat atactttaga
     3721 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3781 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3841 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3901 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3961 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     4021 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4081 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4141 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4201 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4261 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4321 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4381 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4441 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4501 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4561 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4621 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4681 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     4741 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     4801 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     4861 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
     4921 gctctagtcg agcggccgcc agtgtgatgg atatctgtag ttaatgatta acccgccatg
     4981 ctacttatct acgtagccat ggaaactaga taagaaagaa atacgcagag accaaagttc
     5041 aactgaaacg aattaaacgg tttattgatt aacaagcaat tacagattac gagtcaggta
     5101 tctggtgcca atggggcggg gttcactata tacaccttca gtattaacag caaattcaac
     5161 attattagac ttgtaatagt tggaagtgta ctggatctcc gggttccagc gcttgctgtt
     5221 ttccttctgc agctcccact cgatctccac gctgacttgg ccagtagaat actgggtgat
     5281 gaaagagttc agcttgtcct tgttgaaggc cgttggagga tccgcaggta caggtgtgtt
     5341 tttgatgagg atctgaggag gcgggtgctt cattccaaac cctcccatca gcggagaagg
     5401 gtgaaagttg ccgtccgtgt gaggaatttt ggcccaaatg ggtccttgca ggtacacatc
     5461 tctgtcctgc caaaccatac ccggaagtat tccttggttt tgaacccagc cggtctgcgc
     5521 ctgtgcccaa gaagcatgat caccacgttg ggcactctgg tggtttgtgg ccacttgtcc
     5581 ataggactcc gttgctaccg ggttagtagt tttaatttct tcttcgttgg ttatcatgac
     5641 tttgtccgca tccacgttgt ctcttccagt tccttgtttg ccaaaaatta aagatccaga
     5701 caaaggaaag aaacggtcct ctccttcttt gtggctggcc atagcaggtc caggattcat
     5761 caagctatta cgtccattga gagcccaaga agaagctcca ggccaagcaa attcgctgtt
     5821 gttgttttga gtcacagtgg ttgagacacg ttgttgtcgg tagctgggtc caggtatgta
     5881 gtttcttccc tggacagcca tgttgctggg tccggccaca ctgaatttta gcgtttgttg
     5941 attctgtcca gaaccgttaa tagtctttga gagatagtac aagtattggt cgatgagtgg
     6001 attcattagt cggtccaggc tttggctgtg agcgtagctg ctatggaaag gtacgttctc
     6061 aaactcgtag ctgaactgga agttgttacc cgttcttagc atttgcgacg ggaaatattc
     6121 caggcagtaa aaggacgaac gacccacggc ctggcttcca tcattaagcg tcagataccc
     6181 gtactgagga atcatgaaaa cgtccgctgg gaacggcggg aggcagccct cgtgagccga
     6241 cccgagcacg tacgggagct gatagtctga gtccgtgaag acctggaccg tgctggtaag
     6301 gttattggcg atggtcttga ctccattgtt gtccgtaacc tctttgacct gaatgttgaa
     6361 gagcttgaag ttgagtcgct taggccggaa tccccagttg ttgttgatga gtcgctgcca
     6421 gtcacgtggt gagaagtggc agtggaatct gttgaagtca aaataccccc agggggtgct
     6481 gtagccgaag taggcgttgt catttgaaga tcctccagat gtgctgttgg agatttgctt
     6541 gtagaggtga ttgttgtagg tgggcagggc ccaggttcgg gtgctggtgg tgatgactct
     6601 gtcccccagc cattgggaat cgcaatgcca atttcccgag gaactaccca ctccatcggc
     6661 accttcgtta ttgtctgcca ctggtgcgcc accacctgaa gccattgtaa gagatcccac
     6721 acctgagggg gctgcgggag gttctccgat tggttgaggg tctgggactg actctgtgtc
     6781 gccagtctga ccgaaattga gtctcttttt agcgggctgt gcacccgatt tgccaatacc
     6841 cgcggaggag tccggttcct gaggagactg ctctacaggc ctcttctttc caggagccgt
     6901 cttagccgct tcctcaacca gaccaagagg ttcaagaagc ctctttttgg cctggaagac
     6961 tgctcgcccg aggttgcccc caaaagacgt atcttctttg agccgctcct ggaactcggc
     7021 gtcggcgtgg ttgtacttga ggtacgggtt gtctccggcc ttgagctgct ggtcgtaggc
     7081 cttgtcgtgc tcgagggccg ccgcgtctgc tgcgttgacc ggctccccct tgtcgagtcc
     7141 gttgccgggt ccaaggtatt tgtaacccgg aagcacaaga cctcgagcgt tgtcttgatg
     7201 ttgttgattt gccttgggtt gaggggctcc aggtttcaaa gcccaccact cgcgaattcc
     7261 ttcactaagg ttgtcctcga gccaatctgg aagataacca tcggcagcca tacctggttt
     7321 aagtcattta ttgttcagaa acacagtcat ccaagtccac attgaccagg tcgcaagcag
     7381 tgcaagcgtc tggcaccttt cccatgatgt gatgaatgta gcacagtttc tgatacgcct
     7441 ttttgacgac agaaacgggt tgagattctg acacgggaaa gcactctaaa cagtctttga
     7501 caccgtgagt gaagcagata tttgaattct gattcagtct ctcgcattgt ctgcagggaa
     7561 acagcatcag attcatgccc acgtgacgag aacatttgtt ttggtacctg tccgcgtagt
     7621 tgatcgaagc tagcggtcgc agaggagcgg gatcaacagt cacgtctgaa ctgcgaggcg
     7681 tctcgggaac aaatgagagc ctggcccgct tctccagact tttatagcta gtattggtga
     7741 cgtcacccag tgggcgtttt agagatttct ccgccccttt agttcccgcc aattccctgg
     7801 gaactttaaa ctcgtgagtc accggcacct gattgacctt tgcccaagca aaaaagtcct
     7861 tgacttcctg cttagtaatc ttgccaaaat ctggcgggag ccgcttagtc agttcaaatt
     7921 tgaacagatc tctctgctta tatagggggt accttctcta tcactgatag ggagctcgag
     7981 acgtgtcgac ttctctatca ctgataggga gacgttctag acgtaccttc tctatcactg
     8041 atagggagct cgagacgtgt cgacttctct atcactgata gggagacgtt ctagacgtac
     8101 cttctctatc actgataggg agctcgagac gtgtcgactt ctctatcacn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gatagggaga cgtcaggtgg cacttgatag
     8281 cttgattcga gccccagctg gttctttccg cctcagaagc catagagccc accgcatccc
     8341 cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc
     8401 ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa
     8461 ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag
     8521 atggctggca actagaaggc acagtcgagg ctgatcagcg agctctagca tttaggtgac
     8581 actatagaat agggccctct agatcgaatt catgcattta cccggggagc atgtcaaggt
     8641 caaaatcgtc aagagcgtca gcaggcagca tatcaaggtc aaagtcgtca agggcatcgg
     8701 ctgggagcat gtctaagtca aaatcgtcaa gggcgtcggc cggcccgccg ctttcgcact
     8761 ttagctgttt ctccaggcca catatgatta gttccaggcc gaaaaggaag gcaggttcgg
     8821 ctccctgccg gtcgaacagc tcaattgctt gtctcagaag tgggggcata gaatcggtgg
     8881 taggtgtctc tctttcctct tttgctactt gatgctcctg ttcctccaat acgcagccca
     8941 gtgtaaagtg gcccacggcg gacagagcgt acagtgcgtt ctccagggag aagccttgct
     9001 gacacaggaa cgcgagctga ttttccaggg tttcgtactg tttctctgtt gggcgggtgc
     9061 cgagatgcac tttagccccg tcgcgatgtg agaggagagc acagcggaat gacttggcgt
     9121 tgttccgcag aaagtcttgc catgactcgc cttccagggg gcagaagtgg gtatgatgcc
     9181 tgtccagcat ctcgatggcc agggcatcga gcagggcccg cttgttcttc acgtgccagt
     9241 acagggtagg ctgctcaact cccagctttt gagcgagttt ccttgtcgtc aggccttcga
     9301 taccgacttc attgagtaat tccagagcgc cgtttatgac tttagattta tccagccgac
     9361 tcatggtggc actagttcac taaggttgtc ctcgagccaa tctggaagat aaccatcggc
     9421 agcaatacct ggtttaagtc atttattgtt cagaaacaca gtcatccaag tccacattga
     9481 ccaggtcgca agcagtgcaa gcgtctggca cctttcccat gatgtgatga atgtagcaca
     9541 gtttctgata cgcctttttg acgacagaaa cgggttgaga ttctgacacg ggaaagcact
     9601 ctaaacagtc tttgacaccg tgagtgaagc agatatttga attctgattc agtctctcgc
     9661 attgtctgca gggaaacagc atcagattca tgcccacgtg acgagaacat ttgttttggt
     9721 acctgtccgc gtagttgatc gaagct
//
