LOCUS       sequence-446445-        8072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 8072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaL
FEATURES             Location/Qualifiers
     source          1..8072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..127
                     /gene="bla"
                     /label=AmpR promoter
     terminator      992..1031
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1062..1650
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1551..1570
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1804..1821
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1836..1975
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1901..1920)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2061..2083
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2077..2268)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2505..2524
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2505..2523
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4912..4929
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(7980..7998)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7994..8041
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ccgcctaatg agcgggcttt tttttaattc ccctatttgt ttatttttct aaatacattc
       61 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      121 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      181 ccttcctgtt tttgctcacc cagaaacgct cgtgaaagta aaagacgcag aggaccaatt
      241 gggggcacga gtgggataca tagaactgga cttgaatagc ggtaaaatcc ttgagagttt
      301 tcgccctgaa gagcgttttc caatgatgag cactttcaaa gttctgctat gtggagcagt
      361 attatcccgt gtagatgcgg ggcaagagca actcggacga cgaatacact attcgcagaa
      421 tgacttggtt gaatactccc cagtgacaga aaagcacctt acggacggaa tgacggtaag
      481 agaattatgt agtgccgcca taacgatgag tgataacact gcggcgaact tacttctgac
      541 aaccatcggt ggaccgaagg aattaaccgc ttttttgcac aatatgggag accatgtaac
      601 tcgccttgac cgttgggaac cagaactgaa tgaagccata ccaaacgacg agcgagacac
      661 cacaatgcct gcggcaatgg caacaacatt acgcaaacta ttaactggcg aactacttac
      721 tctggcttca cggcaacaat taatagactg gcttgaagcg gataaagttg caggaccact
      781 actgcgttcg gcacttcctg ctggctggtt tattgctgat aaatctgggg caggagagcg
      841 tggttcacgg ggtatcattg ccgcacttgg accagatggt aagccttccc gtatcgtagt
      901 tatctacacg acgggtagtc aggcaactat ggacgaacga aatagacaga ttgctgaaat
      961 aggggcttca ctgattaagc attggtaaac cgatacaatt aaaggctcct tttggagcct
     1021 ttttttttgg acggaccggt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1081 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1141 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1201 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1261 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1321 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1381 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1441 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1501 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1561 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1621 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1681 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1741 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1801 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     1861 catctgtgcg gtatttcaca ccgcaatggt gcactctcag tacaatctgc tctgatgccg
     1921 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
     1981 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     2041 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     2101 gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat
     2161 gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct
     2221 tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt
     2281 gtaaggggga tttctgttca tggggtaatg ataccgatga aacgagagag gatgctcacg
     2341 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
     2401 gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt
     2461 aatacagatg taggtgttgg caccatgcat cgatcccgcg aaattaatac gactcactat
     2521 agggagacca caacggtttc cctctagaga tagcttagcg atgtgaaaga agctggagaa
     2581 aactttcaag aaaaagtttc ttcttcagac gataaagaga agtcaaaaaa taagcggaaa
     2641 ataaaaggcg taaatcagat taaaaaagca acggatatta aaagctctac taagattaaa
     2701 agttctaatg atattaaatc atctacagat ttaaaaacaa tgggatcata agcgaaagga
     2761 gaaatgagat atgtctattc aaaaaagtac taatagttca agtttagcag aagtcattga
     2821 ccgtatttta gataaaggaa ttgttattga tgcttttgca agagtttctg ttgtaggaat
     2881 tgaaatttta acgattgaag cgcgagtggt tattgccagt gttgatacat ggttacgcta
     2941 tgcagaagca gtagggcttc ttcgtgacga cgtagaagaa aacggtcttc ctgaacgttc
     3001 aaattcaagt gaagggcagc cgcgttttag tatttaatat gaagtggtat ggaaccttcc
     3061 ttttttgagg gtggttccag cttttagatc taactattgg aggctactaa aaatggaaat
     3121 taaaaaaatt atgcaagccg tgaacgactt tttcggtgaa cacgtagctc ctcctcataa
     3181 aattacctcg gtggaagcta ctgaagatga aggttggaga gttattgttg aagtcattga
     3241 agaacgagaa tatatgaaaa aatacgccaa agatgaaatg ctcggaacgt acgagtgctt
     3301 tgtaaataaa gaaaaagaag tcatttcatt caaacgactc gacgtcagat atagaagcgc
     3361 cattggcatt gaagcataaa cgataagatg gcaggaggaa cgtaaaaatg accgtcttaa
     3421 cagacaaaag gaaaaaaggc agtggagctt ttatacaaga tgacgagaca aaagaggttc
     3481 tttcaagagc gctgagctat ttaaaatccg gctattccat tcattttaca ggtcctgccg
     3541 gcggaggcaa aacctcttta gcgcgagcgc ttgctaaaaa gagaaagcgt cctgtaatgc
     3601 tgatgcacgg gaatcacgag ctcaacaaca aagatttaat tggcgatttt acgggataca
     3661 cgagcaaaaa agtaatcgac cagtacgttc gttctgtcta taaaaaagat gaacaggtga
     3721 gtgaaaactg gcaggatggc cgattgcttg aagctgtaaa aaatggctat acgctgattt
     3781 acgacgaatt tactcgttct aagcctgcga cgaataatat ctttctatcg atattagaag
     3841 aaggcgtgct gccgctgtat ggagtaaaaa tgaccgatcc ttttgtgcgc gtgcatcccg
     3901 atttccgcgt catcttcaca agcaatccag ctgagtatgc cggcgtatat gatacgcaag
     3961 atgcgcttct cgacaggtta attaccatgt ttattgatta taaagacatc gacagagaga
     4021 cagcgatttt aacggagaaa acggacgtag aagaagatga agcgcgcaca attgtaacgc
     4081 tcgtagcaaa cgtgcgaaac cgctctggag acgaaaacag cagcggactt agcctgcggg
     4141 cttcgcttat gatcgctacc cttgccacgc agcaagacat tcctatcgat ggaagtgacg
     4201 aagattttca aacgttatgt atcgatattt tgcatcatcc gcttaccaaa tgtttggatg
     4261 aagaaaatgc aaaaagcaaa gccgaaaaaa tcattttaga agaatgtaag aatatagaca
     4321 ctgaagaaaa gtaaaggagc ttgaaaacat gagtgaaaca aacgaaacag gtatttatat
     4381 ttttagcgcc attcaaacgg ataaagacga agaatttggc gccgtggaag tagaaggaac
     4441 aaaagctgaa acatttttga ttcgctacaa agacgcggct atggtagcag ctgaagtacc
     4501 gatgaaaatt tatcatccta atcgccaaaa tttattaatg catcaaaacg cagtagcagc
     4561 gattatggac aagaacgata cggttattcc aatcagcttt gggaatgtat tcaaatcaaa
     4621 agaagacgta aaagttcttt tggaaaacct ttatccgcag tttgaaaagc tgtttccagc
     4681 gatcaaagga aaaattgaag tcggtttaaa agtaattggg aaaaaagaat ggcttgagaa
     4741 aaaagtaaac gaaaatcctg aacttgagaa agtatcagca tccgtaaaag gaaaatcaga
     4801 agcagccggt tattatgagc gtattcaact tggaggaatg gctcaaaaga tgtttacttc
     4861 cctgcaaaaa gaagtcaaga cagatgtgtt ttctccgctt gaagaagcag cggaagcagc
     4921 aaaagcaaat gagccaacgg gcgaaacgat gcttttaaac gcgtctttct taattaaccg
     4981 agaagatgaa gcgaagtttg atgaaaaagt aaatgaagcg catgaaaact ggaaagacaa
     5041 agccgatttt cattacagcg gtccttggcc tgcttataat tttgtgaaca ttcgcctaaa
     5101 agtagaagag aaataacgtg cttcacaaat tagtaaccgc acccattaac cttgtagtga
     5161 aaatcggcga aaaagtacag gaagaagctg ataaacagct atatgacctt ccgacgattc
     5221 agcaaaagct cattcagctt caaatgatgt ttgagcttgg tgaaattcca gaagaagcgt
     5281 ttcaagaaaa agaagatgaa ttgttaatga ggtacgaaat tgcgaaacgc agagaaattg
     5341 aacaatggga agagctaaca caaaaaagaa atgaggaatc ctagagtgag gaattaacat
     5401 tatgtctctt aaacaatcca tggagaataa agatattgct cttattgata ttttagatgt
     5461 cattttagat aaaggagtcg ccattaaagg agacttaatc atttccatag ctggcgtcga
     5521 tttagtgtat ttggatttgc gggtgcttat ttcttcggtt gaaacgcttg tgcaagcaaa
     5581 agaaggaaat cacaaaccaa tcacttctga acaatttgat aaacaaaaag aagagctgat
     5641 ggacgctacg ggccagccta gtaagtggac gaaccctcta ggttcgtgac ccttgcgagc
     5701 agtcgtatgc tttctcgaat tccgagcggt taagcgtgac agtccaggag gaattaatag
     5761 atgcaaccgg tcagccaagc aaatggacga atccacttgg atcctgatca agctgaacaa
     5821 ggcttagcgc agcttgtgat gacagttatt gagctattga ggcaaatcgt tgaacgtcat
     5881 gccatgaggc gggtggaggg tggaacgttg acggacgaac aaattgaaaa cttaggaatt
     5941 gcactaatga acttagaaga aaaaatggac gagttgaaag aggtgttcgg tctggatgca
     6001 gaagatttaa atattgatct tggcccgcta ggcagcctgc tttaagcggt cagtaggagg
     6061 aacagtatgg cagtcgaaca taatatgcag tcaagtacga ttgtagatgt gctcgaaaag
     6121 attttggata aaggagtcgt tatagcgggg gacatcaccg taggaattgc agatgtcgag
     6181 ctattaacga taaagatccg cttgattgtg gcttcggttg ataaggcaaa agaaatcggc
     6241 atggactggt gggaaaatga tccgtatctc agttcaaaag gagccaataa caaagcgctc
     6301 gaagaagaaa ataaaatgct gcatgagcgg ttaaaaacgc ttgaagaaaa aatagaaacg
     6361 aaacgttaaa aactgtacgc tacttaaaaa atggagggat ttacaatggc aactgaaaca
     6421 aaattagata acacacaggc agaaaacaag gaaaataaaa atgcggaaaa cggttcaaaa
     6481 gaaaagaacg gttcaaaagc aagcaaaaca acaagcagcg ggccaatcaa acgagcggta
     6541 gcaggaggca tcatcggtgc aacgattgga tatgtatcga ctcctgaaaa tcgaaaaagt
     6601 ctccttgacc gcattgatac agacgaatta aaaagcaaag catctgattt aggaacaaag
     6661 gtaaaagaaa aatcaaaaag cagcgtggcc agcctgaaaa catctgcggg aagcttgttt
     6721 aaaaaagata aagataaatc aaaagatgat gaagaaaacg taaattcttc tagtagcgaa
     6781 acagaagacg ataacgttca agagtacgac gagttaaaag aagaaaatca aactcttcaa
     6841 gatcgcttat cacagcttga agaaaaaatg aacatgcttg ttgagcttag cctcaataaa
     6901 aatcaagacg aagaagcgga agatacagat tccgacgaag aagagaacga tgagaacgat
     6961 gaaaacgatg aaaacgagca ggacgatgaa aacgaagaag aaacatctaa gccacgtaaa
     7021 aaggataaaa aagaagctga ggaagaagaa agtgaaagtg acgaagacag cgaggaagaa
     7081 gaggaagatt ctcgctcaaa caaaaaaaat aaaaaagtaa aaacagaaga agaagacgaa
     7141 gatgaaagcg aagaagaaaa aaaggaagcg aaaccaaaaa agtcaacagc taaaaaatca
     7201 aaaaatacaa aagcaaagaa aaacacggac gaagaagatg atgaagcaac atctctttct
     7261 agtgaagacg atacaacagc ctaagacgta aaggaggaaa gaaagacatg agtacaggcc
     7321 cttctttttc aactaaagac aatacgcttg aatactttgt gaaagcttct aataaacacg
     7381 gcttttcact tgatatttca ttaaatgtaa acggcgctgt gatttccggt accatgattt
     7441 cagcaaaaga atactttgat tacttaagcg aaacgtttga agaaggcagt gaagtggctc
     7501 aggcgctaag cgaacaattc tctttagcaa gcgaagcgag cgaatcaaac ggagaagcag
     7561 aagcccattt tattcatttg aaaaatacaa agatttactg tggagacagt aaatctactc
     7621 cttctaaagg caaaatcttt tggagaggga aaatagcaga agtagacggg tttttcttag
     7681 gaaagatttc tgatgcaaaa tcaacgagta aaaagagttc ataaaaaacg gcggggtgat
     7741 tgccccgccg ttttttagtg atgtgatgag atgtgcagct tcttttttcg atacatgagc
     7801 ggagtcatct tcatttcttt tcgaaactga ttaataaaat agcttgtact attaaaccct
     7861 acttgatagg cgacttccgt cacatttgct tctgcttgct gcagtccgga ttcgcgaacg
     7921 cgtctcgaga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
     7981 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     8041 gctgaaagga ggaactatat ccgactagta gc
//
