LOCUS       sequence-446449-        9551 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Rep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV
            capsid in mice and macaques..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Tabebordbar M, Lagerborg KA, Stanton A, King EM, Ye S, Tellez L, 
            Krunnfusz A, Tavakoli S, Widrick JJ, Messemer KA, Troiano EC, 
            Moghadaszadeh B, Peacker BL, Leacock KA, Horwitz N, Beggs AH, Wagers
            AJ, Sabeti PC
  TITLE     Directed evolution of a family of AAV capsid variants enabling 
            potent muscle-directed gene delivery across species.
  JOURNAL   Cell. 2021 Sep 16;184(19):4919-4938.e22. doi: 
            10.1016/j.cell.2021.08.028. Epub 2021 Sep 9.
  PUBMED    34506722
REFERENCE   2  (bases 1 to 9551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.08.028"; journalName: "Cell"; date: 
            "2021-09-16- 16"; volume: "184"; issue: "19"; pages: "4919-4938.e22"
COMMENT     Sequence Label: kiCAP-AAV-MyoAAV4A
FEATURES             Location/Qualifiers
     source          1..9551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2199..2210
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(2551..2571)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2551..2569)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2590..2606)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2590..2606)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2603..2625)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2614..2630
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2638..2644,2645..2662,2663..2668))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2683..2704
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2821..2838)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2992..3580)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3072..3091)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3751..4560)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4474..4493
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(4561..4665)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4691..5146)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4828..4849)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5040..5059
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5273..5295
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5287..5304
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5288..5304
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5314..5333
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5314..5332
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8124..8234
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     promoter        complement(8273..8291)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8274..8291)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8311..8328)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8317..8541
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    8603..8621
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8640..8658
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8677..8695
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8714..8732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8751..8769
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     gap             8799..8898
                     /estimated_length=100
ORIGIN
        1 gtatcagaaa ctgtgctaca ttcatcacat catgggaaag gtgccagacg cttgcactgc
       61 ttgcgacctg gtcaatgtgg acttggatga ctgtgtttct gaacaataaa tgacttaaac
      121 caggtatggc tgccgatggt tatcttccag attggctcga ggacaacctt agtgaaggaa
      181 ttcgcgagtg gtgggctttg aaacctggag cccctcaacc caaggcaaat caacaacatc
      241 aagacaacgc tcgaggtctt gtgcttccgg gttacaaata ccttggaccc ggcaacggac
      301 tcgacaaggg ggagccggtc aacgcagcag acgcggcggc cctcgagcac gacaaggcct
      361 acgaccagca gctcaaggcc ggagacaacc cgtacctcaa gtacaaccac gccgacgccg
      421 agttccagga gcggctcaaa gaagatacgt cttttggggg caacctcggg cgagcagtct
      481 tccaggccaa aaagaggctt cttgaacctc ttggtctggt tgaggaagcg gctaagacgg
      541 ctcctggaaa gaagaggcct gtagagcagt ctcctcagga accggactcc tccgcgggta
      601 ttggcaaatc gggtgcacag cccgctaaaa agagactcaa tttcggtcag actggcgaca
      661 cagagtcagt cccagaccct caaccaatcg gagaacctcc cgcagccccc tcaggtgtgg
      721 gatctcttac aatggcttca ggtggtggcg caccagtggc agacaataac gaaggtgccg
      781 atggagtggg tagttcctcg ggaaattggc attgcgattc ccaatggctg ggggacagag
      841 tcatcaccac cagcacccga acctgggccc tgcccaccta caacaatcac ctctacaagc
      901 aaatctccaa cagcacatct ggaggatctt caaatgacaa cgcctacttc ggctacagca
      961 ccccctgggg gtattttgac ttcaacagat tccactgcca cttctcacca cgtgactggc
     1021 agcgactcat caacaacaac tggggattcc ggcctaagcg actcaacttc aagctcttca
     1081 acattcaggt caaagaggtt acggacaaca atggagtcaa gaccatcgcc aataacctta
     1141 ccagcacggt ccaggtcttc acggactcag actatcagct cccgtacgtg ctcgggtcgg
     1201 ctcacgaggg ctgcctcccg ccgttcccag cggacgtttt catgattcct cagtacgggt
     1261 atctgacgct taatgatgga agccaggccg tgggtcgttc gtccttttac tgcctggaat
     1321 atttcccgtc gcaaatgcta agaacgggta acaacttcca gttcagctac gagtttgaga
     1381 acgtaccttt ccatagcagc tacgctcaca gccaaagcct ggaccgacta atgaatccac
     1441 tcatcgacca atacttgtac tatctctcaa agactattaa cggttctgga cagaatcaac
     1501 aaacgctaaa attcagtgtg gccggaccca gcaacatggc tgtccaggga agaaactaca
     1561 tacctggacc cagctaccga caacaacgtg tctcaaccac tgtgactcaa aacaacaaca
     1621 gcgaatttgc ttggcctgga gcttcttctt gggctctcaa tggacgtaat agcttgatga
     1681 atcctggacc tgctatggcc agccacaaag aaggagagga ccgtttcttt cctttgtctg
     1741 gatctttaat ttttggcaaa caaggaactg gaagagacaa cgtggatgcg gacaaagtca
     1801 tgataaccaa cgaagaagaa attaaaacta ctaacccggt agcaacggag tcctatggac
     1861 aagtggccac aaaccaccag tcaaactcca gaggagacta caactccttg gcacaggcgc
     1921 agaccggctg ggttcaaaac caaggaatac ttccgggtat ggtttggcag gacagagatg
     1981 tgtacctgca aggacccatt tgggccaaaa ttcctcacac ggacggcaac tttcaccctt
     2041 ctccgctgat gggagggttt ggaatgaagc acccgcctcc tcagatcctc atcaaaaaca
     2101 cacctgtacc tgcggatcct ccaacggcct tcaacaagga caagctgaac tctttcatca
     2161 cccagtattc tactggccaa gtcagcgtgg agatcgagtg ggagctgcag aaggaaaaca
     2221 gcaagcgctg gaacccggag atccagtaca cttccaacta ttacaagtct aataatgttg
     2281 aatttgctgt taatactgaa ggtgtatata gtgaaccccg ccccattggc accagatacc
     2341 tgactcgtaa tctgtaattg cttgttaatc aataaaccgt ttaattcgtt tcagttgaac
     2401 tttggtctct gcgtatttct ttcttatcta gtttccatgg ctacgtagat aagtagcatg
     2461 gcgggttaat cattaactac agatatccat cacactggcg gccgctcgac tagagcggcc
     2521 gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     2581 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     2641 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     2701 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     2761 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     2821 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2881 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2941 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3001 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3061 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3121 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3181 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3241 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3301 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3361 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3421 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3481 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3541 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3601 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3661 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3721 aagtatatat gagtaaactt ggtctgacag ttagaaaaac tcatcgagca tcaaatgaaa
     3781 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     3841 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     3901 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     3961 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     4021 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     4081 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     4141 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     4201 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgtttttcc
     4261 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     4321 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     4381 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     4441 gcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     4501 atcagcatcc atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat
     4561 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4621 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4681 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     4741 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     4801 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     4861 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     4921 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     4981 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     5041 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     5101 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     5161 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     5221 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     5281 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     5341 ggtaccgggc cccccctcga tcgaggtcga cacgcgtgga ggggtggagt cgtgacgtga
     5401 attacgtcat agggttaggg aggtcctgta ttagaggtca cgtgagtgtt ttgcgacatt
     5461 ttgcgacacc atgtggtcac gctgggtatt taagcccgag tgagcacgca gggtctccat
     5521 tttgaagcgg gaggtttgaa cgcgcagccg ccatgccggg gttttacgag attgtgatta
     5581 aggtccccag cgaccttgac gagcatctgc ccggcatttc tgacagcttt gtgaactggg
     5641 tggccgagaa ggaatgggag ttgccgccag attctgacat ggatctgaat ctgattgagc
     5701 aggcacccct gaccgtggcc gagaagctgc agcgcgactt tctgacggaa tggcgccgtg
     5761 tgagtaaggc cccggaggct cttttctttg tgcaatttga gaagggagag agctacttcc
     5821 acatgcacgt gctcgtggaa accaccgggg tgaaatccat ggttttggga cgtttcctga
     5881 gtcagattcg cgaaaaactg attcagagaa tttaccgcgg gatcgagccg actttgccaa
     5941 actggttcgc ggtcacaaag accagaaatg gcgccggagg cgggaacaag gtggtggatg
     6001 agtgctacat ccccaattac ttgctcccca aaacccagcc tgagctccag tgggcgtgga
     6061 ctaatatgga acagtattta agcgcctgtt tgaatctcac ggagcgtaaa cggttggtgg
     6121 cgcagcatct gacgcacgtg tcgcagacgc aggagcagaa caaagagaat cagaatccca
     6181 attctgatgc gccggtgatc agatcaaaaa cttcagccag gtacatggag ctggtcgggt
     6241 ggctcgtgga caaggggatt acctcggaga agcagtggat ccaggaggac caggcctcat
     6301 acatctcctt caatgcggcc tccaactcgc ggtcccaaat caaggctgcc ttggacaatg
     6361 cgggaaagat tatgagcctg actaaaaccg cccccgacta cctggtgggc cagcagcccg
     6421 tggaggacat ttccagcaat cggatttata aaattttgga actaaacggg tacgatcccc
     6481 aatatgcggc ttccgtcttt ctgggatggg ccacgaaaaa gttcggcaag aggaacacca
     6541 tctggctgtt tgggcctgca actaccggga agaccaacat cgcggaggcc atagcccaca
     6601 ctgtgccctt ctacgggtgc gtaaactgga ccaatgagaa ctttcccttc aacgactgtg
     6661 tcgacaagat ggtgatctgg tgggaggagg ggaagatgac cgccaaggtc gtggagtcgg
     6721 ccaaagccat tctcggagga agcaaggtgc gcgtggacca gaaatgcaag tcctcggccc
     6781 agatagaccc gactcccgtg atcgtcacct ccaacaccaa catgtgcgcc gtgattgacg
     6841 ggaactcaac gaccttcgaa caccagcagc cgttgcaaga ccggatgttc aaatttgaac
     6901 tcacccgccg tctggatcat gactttggga aggtcaccaa gcaggaagtc aaagactttt
     6961 tccggtgggc aaaggatcac gtggttgagg tggagcatga attctacgtc aaaaagggtg
     7021 gagccaagaa aagacccgcc cccagtgacg cagatataag tgagcccaaa cgggtgcgcg
     7081 agtcagttgc gcagccatcg acgtcagacg cggaagcttc gatcaactac gcggacaggt
     7141 accaaaacaa atgttctcgt cacgtgggca tgaatctgat gctgtttccc tgcagacaat
     7201 gcgagagact gaatcagaat tcaaatatct gcttcactca cggtgtcaaa gactgtttag
     7261 agtgctttcc cgtgtcagaa tctcaacccg tttctgtcgt caaaaaggcg tatcagaaac
     7321 tgtgctacat tcatcacatc atgggaaagg tgccagacgc ttgcactgct tgcgacctgg
     7381 tcaatgtgga cttggatgac tgtgtttctg aacaataaat gacttaaacc aggtattgct
     7441 gccgatggtt atcttccaga ttggctcgag gacaacctta gtgaactagt gccaccatga
     7501 gtcggctgga taaatctaaa gtcataaacg gcgctctgga attactcaat gaagtcggta
     7561 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     7621 actggcacgt gaagaacaag cgggccctgc tcgatgccct ggccatcgag atgctggaca
     7681 ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     7741 acgccaagtc attccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     7801 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     7861 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     7921 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
     7981 ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag
     8041 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     8101 agtgcgaaag cggcgggccg gccgacgccc ttgacgattt tgacttagac atgctcccag
     8161 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     8221 accttgacat gctccccggg taaatgcatg aattcgatct agagggccct attctatagt
     8281 gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     8341 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     8401 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     8461 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     8521 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgaatcaag
     8581 ctatcaagtg ccacctgacg tctccctatc agtgatagag aagtcgacac gtctcgagct
     8641 ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat agagaagtcg
     8701 acacgtctcg agctccctat cagtgataga gaaggtacgt ctagaacgtc tccctatcag
     8761 tgatagagaa gtcgacacgt ctcgagctcc ctatcagtnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnga tagagaaggt accccctata taagcagaga gatctgttca
     8941 aatttgaact gactaagcgg ctcccgccag attttggcaa gattactaag caggaagtca
     9001 aggacttttt tgcttgggca aaggtcaatc aggtgccggt gactcacgag tttaaagttc
     9061 ccagggaatt ggcgggaact aaaggggcgg agaaatctct aaaacgccca ctgggtgacg
     9121 tcaccaatac tagctataaa agtctggaga agcgggccag gctctcattt gttcccgaga
     9181 cgcctcgcag ttcagacgtg actgttgatc ccgctcctct gcgaccgcta gcttcgatca
     9241 actacgcgga caggtaccaa aacaaatgtt ctcgtcacgt gggcatgaat ctgatgctgt
     9301 ttccctgcag acaatgcgag agactgaatc agaattcaaa tatctgcttc actcacggtg
     9361 tcaaagactg tttagagtgc tttcccgtgt cagaatctca acccgtttct gtcgtcaaaa
     9421 aggcgtatca gaaactgtgc tacattcatc acatcatggg aaaggtgcca gacgcttgca
     9481 ctgcttgcga cctggtcaat gtggacttgg atgactgtgt ttctgaacaa taaatgactt
     9541 aaaccaggta t
//
