LOCUS       sequence-446457-        9074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing human LMNB1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9074)
  TITLE     Lentiviral plasmids expressing popular genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti puro HA-LMNB1
FEATURES             Location/Qualifiers
     source          1..9074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     49..69
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        51..69
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        97..323
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             324..504
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    551..676
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1169..1402
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1587..1631
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1929..1949
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        2238..2441
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2392..2412
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2489..2515
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    4361..4478
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4527..5037
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4956..4974
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5059..5658
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5059..5078)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5555..5575
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5786..6019
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6091..6212
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6128..6147)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6182..6201
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6252..6387
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6407..6426)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6408..6426)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6458..6475)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6458..6474)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6467..6489)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6683..6702)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6802..6824
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6862..6880)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6948..7052
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7053..7121,7122..7913)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7271..7290)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8084..8672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8573..8592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8826..8843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8960..8981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8996..9001,9002..9019,9020..9026)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9034..9050
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9039..9061
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9058..9074
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9058..9074
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgattacg ccaagcttgc atgcctgcag catgattacg ccaagcgcgc aattaaccct
       61 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
      121 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
      181 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
      241 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
      301 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
      361 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      421 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      481 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      541 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      601 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      661 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
      721 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
      781 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
      841 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
      901 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
      961 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1021 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1081 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1141 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1201 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1261 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1321 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1381 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1441 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1501 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1561 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1621 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1681 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     1741 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     1801 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     1861 ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga gcggccgccc
     1921 ccttcaccga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
     1981 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     2041 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     2101 actatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2161 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2221 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     2281 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     2341 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     2401 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
     2461 tccgctagcg ctaccggtcg ccaccatgta cccatacgat gttccggatt acgctagcgg
     2521 atccatggct actgcgacgc ctgtgcctcc tcgaatgggg tcacgggccg gcggtcctac
     2581 gacaccgttg agcccgacaa gactgagtag gttgcaagaa aaggaagagt tgcgagaact
     2641 taacgatcga ctggcggtct atattgacaa ggttcgctcc ttggagacag agaactcagc
     2701 tttgcagctc caggtaacgg agcgggaaga ggttagaggc cgcgaactca ccgggctgaa
     2761 agctttgtat gagactgaat tggccgatgc acgcagggcc ctggacgata cagcgagaga
     2821 gagggctaaa ctgcaaattg agttgggcaa atgcaaagcc gagcacgacc aacttctcct
     2881 taactacgcg aagaaagaga gtgacttgaa tggtgcccag atcaagcttc gagaatacga
     2941 ggcagccctt aatagcaaag acgccgccct ggccacagca ctcggtgaca aaaagtcctt
     3001 ggagggagac ttggaagacc tcaaagatca gatcgcccaa cttgaggcca gcctggccgc
     3061 ggccaaaaaa caactcgctg atgagaccct gctcaaagtc gatcttgaga acaggtgtca
     3121 atccctgaca gaggatctgg aatttcgaaa aagcatgtac gaagaagaaa ttaacgagac
     3181 acggaggaaa catgaaactc gccttgtgga ggtagattcc ggccggcaaa ttgaatatga
     3241 gtataagctg gctcaggcac tccatgagat gcgcgaacag cacgacgccc aggttaggct
     3301 gtacaaagag gaacttgagc agacctacca tgccaaactt gaaaatgcta gactctctag
     3361 cgagatgaat acgtcaacag taaattcagc tagggaagag ctcatggaat cacggatgcg
     3421 aatcgagtca ctgtccagtc aactgtctaa ccttcagaag gagagtcggg cctgtctgga
     3481 aaggattcag gaactcgagg acctcctggc caaagaaaaa gataatagca ggcgcatgtt
     3541 gacagataag gaacgggaga tggctgaaat tcgcgaccaa atgcaacaac aacttaatga
     3601 ctatgaacaa ctcctcgacg tgaagctcgc actggacatg gagatctcag cgtataggaa
     3661 gctgcttgaa ggggaagaag aaagactgaa gctctccccg tctcccagtt caagggtcac
     3721 cgtctctcga gcgtcctcct ccaggtcagt acgcaccacg cgcggtaaac gaaagcgcgt
     3781 tgacgtagag gagtccgaag cttcctccag cgtgtcaatc agtcattcag cctcagctac
     3841 cggaaatgtc tgcatcgagg aaattgatgt cgacggaaaa tttataaggc ttaaaaatac
     3901 aagtgagcaa gatcaaccca tgggtggttg ggaaatgatc cggaagattg gggacacgag
     3961 cgtcagctat aaatacactt cccggtacgt tcttaaggcc ggtcagacag ttactatctg
     4021 ggctgcaaat gctggggtaa cagcctctcc cccaactgat ttgatctgga agaaccagaa
     4081 ctcatggggg accggagagg acgtgaaggt gatactgaag aattcacaag gagaggaggt
     4141 cgctcagcga agtaccgtgt ttaagacgac aataccagag gaggaagaag aggaggaaga
     4201 ggcagccggt gtcgtcgttg aagaggaact ctttcaccaa cagggtactc ctcgcgccag
     4261 caatcgatcc tgtgccatca tgtagccgga ctcagatctc gagctcaagc ttctctagag
     4321 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     4381 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     4441 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     4501 gagatccact ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct
     4561 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
     4621 cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
     4681 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
     4741 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac
     4801 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc
     4861 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt
     4921 ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg
     4981 gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg
     5041 gatccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5101 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     5161 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     5221 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     5281 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     5341 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     5401 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     5461 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     5521 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     5581 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     5641 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
     5701 cgaccccatg catcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     5761 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     5821 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     5881 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     5941 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6001 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     6061 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     6121 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6181 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     6241 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6301 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6361 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     6421 gtattacgcg cgctccccgg gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc
     6481 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     6541 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     6601 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     6661 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     6721 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     6781 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     6841 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     6901 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6961 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7021 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7081 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7141 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7201 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7261 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7321 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7381 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7441 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7501 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7561 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7621 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7681 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7741 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7801 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7861 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7921 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7981 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8041 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8101 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8161 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8221 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8281 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8341 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8401 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8461 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8521 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8581 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8641 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8701 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8761 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8821 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8881 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     8941 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     9001 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     9061 gaaacagcta tgac
//
