LOCUS       sequence-446476-       19674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, 
            hygromycin and red fluorescent seed selection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19674)
  TITLE     Takeuchi CRISPR/Cas9 Plasmids for multi-gene knockout in Arabidopsis
REFERENCE   2  (bases 1 to 19674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHEE-R-hyg
FEATURES             Location/Qualifiers
     source          1..19674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             531..1238
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     944..963
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2181..2972
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        3124..3199
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4769..4834
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4841..4861
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4886..8986
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4886..8986
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             8899..8907
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8987..9034
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(9691..9707)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9691..9707)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9704..9726)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9715..9731
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9739..9745,9746..9763,9764..9769))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9784..9805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9996..10672
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10609..10629
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             10739..11764
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    11805..11979
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12057..12081
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12506..13300
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14725..15313
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15214..15233
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15467..15484
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15499..15639
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15565..15584)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15725..15747
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      15983..16177
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16243..17316)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17745..18374)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagtttc ttgaaaccaa attaacatag
       61 ggtttttatt taaataaaag tttaaccttc tttttaaaaa attgttcata gtgtcatgtc
      121 agaacaagag ctacaaatca cacatagcat gcataagcgg agctatgatg agtggtattg
      181 ttttgttcgt cacttgtcac tcttttccaa cacataatcc cgacaacaac gtaagagcat
      241 ctctctctct ccacacacac tcatgcatgc atgcattctt acacgtgatt gccatgcaaa
      301 tctcctttct cacctataaa tacaaaccaa cccttcacta cactcttcac tcaaaccaaa
      361 acaagaaaac atacacaaat agcaaaacaa gggcgaattc tcaacacaac atatacaaaa
      421 caaacgaatc tcaagcaatc aagcattcta cttctattgc agcaatttaa atcatttctt
      481 ttaaagcaaa agcaattttc tgaaaatttt caccatttac gaacgatagc atggtgagca
      541 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      601 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      661 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      721 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      781 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      841 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      901 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
      961 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1021 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1081 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1141 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1201 gccgccactc caccggcggc atggacgagc tgtacaagaa ggacgagctg taagcgccgc
     1261 ccggctgcat cgctgaaatc accagtctct ctctacaaat ctatctctct ctataataat
     1321 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     1381 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     1441 ctaattccta aaaccaaaat ccagtggtac caagcttcga cttgccttcc gcacaataca
     1501 tcatttcttc ttagcttttt ttcttcttct tcgttcatac agtttttttt tgtttatcag
     1561 cttacatttt cttgaaccgt agctttcgtt ttcttctttt taactttcca ttcggagttt
     1621 ttgtatcttg tttcatagtt tgtcccagga ttagaatgat taggcatcga accttcaaga
     1681 atttgattga ataaaacatc ttcattctta agatatgaag ataatcttca aaaggcccct
     1741 gggaatctga aagaagagaa gcaggcccat ttatatggga aagaacaata gtatttctta
     1801 tataggccca tttaagttga aaacaatctt caaaagtccc acatcgctta gataagaaaa
     1861 cgaagctgag tttatataca gctagagtcg aagtagtgat tgggagacca acccagtgga
     1921 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     1981 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2041 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2101 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2161 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     2221 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2281 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2341 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2401 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2461 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2521 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2581 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2641 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2701 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2761 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2821 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2881 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     2941 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     3001 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     3061 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccggtc
     3121 tcggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     3181 agtggcaccg agtcggtgct tttttttgca aaattttcca gatcgatttc ttcttcctct
     3241 gttcttcggc gttcaatttc tggggttttc tcttcgtttt ctgtaactga aacctaaaat
     3301 ttgacctaaa aaaaatctca aataatatga ttcagtggtt ttgtactttt cagttagttg
     3361 agttttgcag ttccgatgag ataaaccaat accatggtta tactagtgaa taaaagcatt
     3421 tgcgtttggt ttatcattgc gtttatacaa ggacagagat ccactgagct ggaatagctt
     3481 aaaaccatta tcagaacaaa ataaaccatt ttttgttaag aatcagagca tagtaaacaa
     3541 cagaaacaac ctaagagagg taacttgtcc aagaagatag ctaattatat ctattttata
     3601 aaagttatca tagtttgtaa gtcacaaaag atgcaaataa cagagaaact aggagacttg
     3661 agaatataca ttcttgtata tttgtattcg agattgtgaa aatttgacca taagtttaaa
     3721 ttcttaaaaa gatatatctg atctaggtga tggttataga ctgtaatttt accacatgtt
     3781 taatgatgga tagtgacaca catgacacat cgacaacact atagcatctt atttagatta
     3841 caacatgaaa tttttctgta atacatgtct ttgtacataa tttaaaagta attcctaaga
     3901 aatatattta tacaaggagt ttaaagaaaa catagcataa agttcaatga gtagtaaaaa
     3961 ccatatacag tatatagcat aaagttcaat gagtttatta caaaagcatt ggttcacttt
     4021 ctgtaacacg acgttaaacc ttcgtctcca ataggagcgc tactgattca acatgccaat
     4081 atatactaaa tacgtttcta cagtcaaatg ctttaacgtt tcatgattaa gtgactattt
     4141 accgtcaatc ctttcccatt cctcccacta atccaacttt ttaattactc ttaaatcacc
     4201 actaagctag taacgcctat catgaattag ctctactaaa tctagcaacc tttcaaattt
     4261 gcagtattgc aggtgtctct gtgtctttaa aatagttgcc ttatgatttc ttcggtttca
     4321 agatgatcaa atagttatag atttcatgct cacacatgct cattagatgt gtacatactt
     4381 tacttaccca aatctatttt ctcgcaaaga ttttgatggt aaagctgatt tggttctatt
     4441 gaactaaatc aaacgagttt cagactgagt gattctaatc cggcccatta gcccctaaac
     4501 agacccacta attacgcagc ttttaataga gtaattacac ctagtttacc cactaaacca
     4561 ctaagcacta attatctcac aatctaatga gcttccctcg taattacttg ggctttcact
     4621 ctaccattta tttgtaacag tcaagtctct actgtctcta tataaactct ctaaagttaa
     4681 cacacaattc tcatcacaaa caaatcaacc aaagcaactt ctactctttc ttctttcgac
     4741 cttatcaatc tgttgagaaa tctagatgga ttacaaggac cacgacgggg attacaagga
     4801 ccacgacatt gattacaagg atgatgatga caagatggct ccgaagaaga agaggaaggt
     4861 tggcatccac ggggtgccag ctgctgacaa gaagtactcg atcggcctcg atattgggac
     4921 taactctgtt ggctgggccg tgatcaccga cgagtacaag gtgccctcaa agaagttcaa
     4981 ggtcctgggc aacaccgatc ggcattccat caagaagaat ctcattggcg ctctcctgtt
     5041 cgacagcggc gagacggctg aggctacgcg gctcaagcgc accgcccgca ggcggtacac
     5101 gcgcaggaag aatcgcatct gctacctgca ggagattttc tccaacgaga tggcgaaggt
     5161 tgacgattct ttcttccaca ggctggagga gtcattcctc gtggaggagg ataagaagca
     5221 cgagcggcat ccaatcttcg gcaacattgt cgacgaggtt gcctaccacg agaagtaccc
     5281 tacgatctac catctgcgga agaagctcgt ggactccaca gataaggcgg acctccgcct
     5341 gatctacctc gctctggccc acatgattaa gttcaggggc catttcctga tcgaggggga
     5401 tctcaacccg gacaatagcg atgttgacaa gctgttcatc cagctcgtgc agacgtacaa
     5461 ccagctcttc gaggagaacc ccattaatgc gtcaggcgtc gacgcgaagg ctatcctgtc
     5521 cgctaggctc tcgaagtctc ggcgcctcga gaacctgatc gcccagctgc cgggcgagaa
     5581 gaagaacggc ctgttcggga atctcattgc gctcagcctg gggctcacgc ccaacttcaa
     5641 gtcgaatttc gatctcgctg aggacgccaa gctgcagctc tccaaggaca catacgacga
     5701 tgacctggat aacctcctgg cccagatcgg cgatcagtac gcggacctgt tcctcgctgc
     5761 caagaatctg tcggacgcca tcctcctgtc tgatattctc agggtgaaca ccgagattac
     5821 gaaggctccg ctctcagcct ccatgatcaa gcgctacgac gagcaccatc aggatctgac
     5881 cctcctgaag gcgctggtca ggcagcagct ccccgagaag tacaaggaga tcttcttcga
     5941 tcagtcgaag aacggctacg ctgggtacat tgacggcggg gcctctcagg aggagttcta
     6001 caagttcatc aagccgattc tggagaagat ggacggcacg gaggagctgc tggtgaagct
     6061 caatcgcgag gacctcctga ggaagcagcg gacattcgat aacggcagca tcccacacca
     6121 gattcatctc ggggagctgc acgctatcct gaggaggcag gaggacttct accctttcct
     6181 caaggataac cgcgagaaga tcgagaagat tctgactttc aggatcccgt actacgtcgg
     6241 cccactcgct aggggcaact cccgcttcgc ttggatgacc cgcaagtcag aggagacgat
     6301 cacgccgtgg aacttcgagg aggtggtcga caagggcgct agcgctcagt cgttcatcga
     6361 gaggatgacg aatttcgaca agaacctgcc aaatgagaag gtgctcccta agcactcgct
     6421 cctgtacgag tacttcacag tctacaacga gctgactaag gtgaagtatg tgaccgaggg
     6481 catgaggaag ccggctttcc tgtctgggga gcagaagaag gccatcgtgg acctcctgtt
     6541 caagaccaac cggaaggtca cggttaagca gctcaaggag gactacttca agaagattga
     6601 gtgcttcgat tcggtcgaga tctctggcgt tgaggaccgc ttcaacgcct ccctggggac
     6661 ctaccacgat ctcctgaaga tcattaagga taaggacttc ctggacaacg aggagaatga
     6721 ggatatcctc gaggacattg tgctgacact cactctgttc gaggaccggg agatgatcga
     6781 ggagcgcctg aagacttacg cccatctctt cgatgacaag gtcatgaagc agctcaagag
     6841 gaggaggtac accggctggg ggaggctgag caggaagctc atcaacggca ttcgggacaa
     6901 gcagtccggg aagacgatcc tcgacttcct gaagagcgat ggcttcgcga accgcaattt
     6961 catgcagctg attcacgatg acagcctcac attcaaggag gatatccaga aggctcaggt
     7021 gagcggccag ggggactcgc tgcacgagca tatcgcgaac ctcgctggct cgccagctat
     7081 caagaagggg attctgcaga ccgtgaaggt tgtggacgag ctggtgaagg tcatgggcag
     7141 gcacaagcct gagaacatcg tcattgagat ggcccgggag aatcagacca cgcagaaggg
     7201 ccagaagaac tcacgcgaga ggatgaagag gatcgaggag ggcattaagg agctggggtc
     7261 ccagatcctc aaggagcacc cggtggagaa cacgcagctg cagaatgaga agctctacct
     7321 gtactacctc cagaatggcc gcgatatgta tgtggaccag gagctggata ttaacaggct
     7381 cagcgattac gacgtcgatc atatcgttcc acagtcattc ctgaaggatg actccattga
     7441 caacaaggtc ctcaccaggt cggacaagaa ccggggcaag tctgataatg ttccttcaga
     7501 ggaggtcgtt aagaagatga agaactactg gcgccagctc ctgaatgcca agctgatcac
     7561 gcagcggaag ttcgataacc tcacaaaggc tgagaggggc gggctctctg agctggacaa
     7621 ggcgggcttc atcaagaggc agctggtcga gacacggcag atcactaagc acgttgcgca
     7681 gattctcgac tcacggatga acactaagta cgatgagaat gacaagctga tccgcgaggt
     7741 gaaggtcatc accctgaagt caaagctcgt ctccgacttc aggaaggatt tccagttcta
     7801 caaggttcgg gagatcaaca attaccacca tgcccatgac gcgtacctga acgcggtggt
     7861 cggcacagct ctgatcaaga agtacccaaa gctcgagagc gagttcgtgt acggggacta
     7921 caaggtttac gatgtgagga agatgatcgc caagtcggag caggagattg gcaaggctac
     7981 cgccaagtac ttcttctact ctaacattat gaatttcttc aagacagaga tcactctggc
     8041 caatggcgag atccggaagc gccccctcat cgagacgaac ggcgagacgg gggagatcgt
     8101 gtgggacaag ggcagggatt tcgcgaccgt caggaaggtt ctctccatgc cacaagtgaa
     8161 tatcgtcaag aagacagagg tccagactgg cgggttctct aaggagtcaa ttctgcctaa
     8221 gcggaacagc gacaagctca tcgcccgcaa gaaggactgg gatccgaaga agtacggcgg
     8281 gttcgacagc cccactgtgg cctactcggt cctggttgtg gcgaaggttg agaagggcaa
     8341 gtccaagaag ctcaagagcg tgaaggagct gctggggatc acgattatgg agcgctccag
     8401 cttcgagaag aacccgatcg atttcctgga ggcgaagggc tacaaggagg tgaagaagga
     8461 cctgatcatt aagctcccca agtactcact cttcgagctg gagaacggca ggaagcggat
     8521 gctggcttcc gctggcgagc tgcagaaggg gaacgagctg gctctgccgt ccaagtatgt
     8581 gaacttcctc tacctggcct cccactacga gaagctcaag ggcagccccg aggacaacga
     8641 gcagaagcag ctgttcgtcg agcagcacaa gcattacctc gacgagatca ttgagcagat
     8701 ttccgagttc tccaagcgcg tgatcctggc cgacgcgaat ctggataagg tcctctccgc
     8761 gtacaacaag caccgcgaca agccaatcag ggagcaggct gagaatatca ttcatctctt
     8821 caccctgacg aacctcggcg cccctgctgc tttcaagtac ttcgacacaa ctatcgatcg
     8881 caagaggtac acaagcacta aggaggtcct ggacgcgacc ctcatccacc agtcgattac
     8941 cggcctctac gagacgcgca tcgacctgtc tcagctcggg ggcgacaagc ggccagcggc
     9001 gacgaagaag gcggggcagg cgaagaagaa gaagtgagct cagagctttc gttcgtatca
     9061 tcggtttcga caacgttcgt caagttcaat gcatcagttt cattgcgcac acaccagaat
     9121 cctactgagt ttgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg
     9181 cttgtaattt actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga
     9241 tggagaagag ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt
     9301 tttttctctt atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt
     9361 tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat gaggagtaaa
     9421 acacttgtag ttgtaccatt atgcttattc actaggcaac aaatatattt tcagacctag
     9481 aaaagctgca aatgttactg aatacaagta tgtcctcttg tgttttagac atttatgaac
     9541 tttcctttat gtaattttcc agaatccttg tcagattcta atcattgctt tataattata
     9601 gttatactca tggatttgta gttgagtatg aaaatatttt ttaatgcatt ttatgacttg
     9661 ccaattgatt gacaacgaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     9721 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9781 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9841 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     9901 tattggctag agcagcttgc caacatggtg gagcacgaca ctctcgtcta ctccaagaat
     9961 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata
    10021 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta
    10081 gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
    10141 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
    10201 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgaaca tggtggagca
    10261 cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat
    10321 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
    10381 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
    10441 cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc
    10501 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
    10561 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
    10621 agacccttcc tctatataag gaagttcatt tcatttggag aggacacgct gaaatcacca
    10681 gtctctctct acaaatctat ctctctcgag ctttcgcaga tccggggggc aatgagatat
    10741 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
    10801 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
    10861 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
    10921 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
    10981 ggagtttagc gagagcctga cctattgcat ctcccgccgt tcacagggtg tcacgttgca
    11041 agacctgcct gaaaccgaac tgcccgctgt tctacaaccg gtcgcggagg ctatggatgc
    11101 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
    11161 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
    11221 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
    11281 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
    11341 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
    11401 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
    11461 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccacg
    11521 actccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
    11581 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
    11641 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
    11701 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagaa
    11761 atagagtaga tgccgaccgg gatctgtcga tcgacaagct cgagtttctc cataataatg
    11821 tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag
    11881 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
    11941 taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa ttcggcgtta
    12001 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
    12061 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
    12121 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
    12181 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
    12241 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
    12301 gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact atgttatacg
    12361 ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt tagaatgcaa
    12421 ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc tttaaatact
    12481 gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg aattgaaaaa
    12541 actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata
    12601 taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg
    12661 gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc
    12721 tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga
    12781 ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat
    12841 cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta
    12901 tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata acgatctggc
    12961 cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc gcgagctgta
    13021 tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg
    13081 agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag
    13141 cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca gggaggatat
    13201 cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga
    13261 gaaaataaaa tattatattt tactggatga attgttttag tacctagaat gcatgaccaa
    13321 aatcccttaa cgtgagtttt cgttccactg agcgctgaga gatcccctca taatttcccc
    13381 aaagcgtaac catgtgtgaa taaattttga gctagtaggg ttgcagccac gagtaagtct
    13441 tcccttgtta ttgtgtagcc agaatgccgc aaaacttcca tgcctaagcg aactgttgag
    13501 agtacgtttc gatttctgac tgtgttagcc tggaagtgct tgtcccaacc ttgtttctga
    13561 gcatgaacgc ccgcaagcca acatgttagt tgaagcatca gggcgattag cagcatgata
    13621 tcaaaacgct ctgagctgct cgttcggcta tggcgtaggc ctagtccgta ggcaggactt
    13681 ttcaagtctc ggaaggtttc ttcaatctgc attcgcttcg aatagatatt aacaagttgt
    13741 ttgggtgttc gaatttcaac aggtaagtta gttgctagaa cccatggctc ctttgccgac
    13801 gctgagtaga ttttaggtga cgggtggtga caatgagtcc gtgtcgagcg ctgatttttt
    13861 cggcctttag agcgagattt atacaataga atttggcatg agattggatt gcttttagtc
    13921 agcctcttat agcctaaagt ctttgagtga ctagatgaca tatcatgtaa gttgctgata
    13981 ggtttccagt tttccgctcc taggtctgca tattgtactt ttcctcttac tcgacttaac
    14041 cagtaccaac ccagcttctc aacggattta taccatggca ctttaaagcc agcatcactg
    14101 acaatgagcg gtgtggtgtt actcggtaga atgctcgcaa ggtcggctag aaattggtca
    14161 tgagctttct ttgaacattg ctctgaaagc gggaacgctt tctcataaag agtaacagaa
    14221 cgaccgtgta gtgcgactga agctcgcaat accataagtc gtttttgctc acgaatatca
    14281 gaccagtcaa caagtacaat gggcatcgta ttgcccgaac agataaagct agcatgccaa
    14341 cggtatacag cgagtcgctc tttgtggagg tgacgattac ctaacaatcg gtcgattcgt
    14401 ttgatgttat gttttgttct cgctttggtt ggcaggttac ggccaagttc ggtaagagtg
    14461 agagttttac agtcaagtaa tgcgtggcaa gccaacgtta agctgttgag tcgttttaag
    14521 tgtaattcgg ggcagaattg gtaaagagag tcgtgtaaaa tatcgagttc gcacatcttg
    14581 ttgtctgatt attgattttt cgcgaaacca tttgatcata tgacaagatg tgtatccacc
    14641 ttaacttaat gatttttacc aaaatcatta ggggattcat cagcactgag cgtcagaccc
    14701 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    14761 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    14821 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
    14881 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    14941 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    15001 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    15061 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    15121 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    15181 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    15241 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    15301 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    15361 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    15421 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    15481 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
    15541 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
    15601 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
    15661 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
    15721 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
    15781 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg
    15841 tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg
    15901 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc
    15961 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag
    16021 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat
    16081 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat
    16141 gtacggcttt gggttcccaa tgtacgtgct atccacagga aacagacctt ttcgaccttt
    16201 ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc
    16261 ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc
    16321 ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca
    16381 gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct
    16441 ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg
    16501 atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat
    16561 ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct
    16621 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc
    16681 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt
    16741 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc
    16801 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt
    16861 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc
    16921 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac
    16981 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg
    17041 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc
    17101 cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg
    17161 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg
    17221 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc
    17281 gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt
    17341 ccagtgccat tgcagggccg gcaggcaacc cagccgctta cgcctggcca accgcccgtt
    17401 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg
    17461 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag
    17521 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat
    17581 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt
    17641 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact
    17701 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat
    17761 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca
    17821 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac
    17881 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg
    17941 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg
    18001 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc
    18061 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg
    18121 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc
    18181 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg
    18241 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg
    18301 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca
    18361 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg
    18421 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc
    18481 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac
    18541 cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc
    18601 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt
    18661 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc
    18721 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac
    18781 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc
    18841 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc
    18901 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc
    18961 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt
    19021 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag
    19081 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc
    19141 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct
    19201 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca
    19261 gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg
    19321 ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta
    19381 gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg
    19441 cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg
    19501 ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact
    19561 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
    19621 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttag
//
