LOCUS       sequence-446477-       19447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, 
            kanamycin and red fluorescent seed selection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19447)
  TITLE     Takeuchi CRISPR/Cas9 Plasmids for multi-gene knockout in Arabidopsis
REFERENCE   2  (bases 1 to 19447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHEE-R-kan
FEATURES             Location/Qualifiers
     source          1..19447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             531..1238
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     944..963
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2181..2972
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        3124..3199
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4769..4834
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4841..4861
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4886..8986
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4886..8986
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             8899..8907
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8987..9034
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(9680..9696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9680..9696)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9693..9715)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9704..9720
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9728..9734,9735..9752,9753..9758))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9773..9794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9985..10661
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10598..10618
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             10724..11521
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10781..10800)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11391..11410
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11578..11752
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11830..11854
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12279..13073
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14498..15086
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14987..15006
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15240..15257
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15272..15412
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15338..15357)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15498..15520
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      15756..15950
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16016..17089)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17518..18147)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagtttc ttgaaaccaa attaacatag
       61 ggtttttatt taaataaaag tttaaccttc tttttaaaaa attgttcata gtgtcatgtc
      121 agaacaagag ctacaaatca cacatagcat gcataagcgg agctatgatg agtggtattg
      181 ttttgttcgt cacttgtcac tcttttccaa cacataatcc cgacaacaac gtaagagcat
      241 ctctctctct ccacacacac tcatgcatgc atgcattctt acacgtgatt gccatgcaaa
      301 tctcctttct cacctataaa tacaaaccaa cccttcacta cactcttcac tcaaaccaaa
      361 acaagaaaac atacacaaat agcaaaacaa gggcgaattc tcaacacaac atatacaaaa
      421 caaacgaatc tcaagcaatc aagcattcta cttctattgc agcaatttaa atcatttctt
      481 ttaaagcaaa agcaattttc tgaaaatttt caccatttac gaacgatagc atggtgagca
      541 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      601 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      661 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      721 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      781 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      841 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      901 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
      961 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1021 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1081 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1141 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1201 gccgccactc caccggcggc atggacgagc tgtacaagaa ggacgagctg taagcgccgc
     1261 ccggctgcat cgctgaaatc accagtctct ctctacaaat ctatctctct ctataataat
     1321 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     1381 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     1441 ctaattccta aaaccaaaat ccagtggtac caagcttcga cttgccttcc gcacaataca
     1501 tcatttcttc ttagcttttt ttcttcttct tcgttcatac agtttttttt tgtttatcag
     1561 cttacatttt cttgaaccgt agctttcgtt ttcttctttt taactttcca ttcggagttt
     1621 ttgtatcttg tttcatagtt tgtcccagga ttagaatgat taggcatcga accttcaaga
     1681 atttgattga ataaaacatc ttcattctta agatatgaag ataatcttca aaaggcccct
     1741 gggaatctga aagaagagaa gcaggcccat ttatatggga aagaacaata gtatttctta
     1801 tataggccca tttaagttga aaacaatctt caaaagtccc acatcgctta gataagaaaa
     1861 cgaagctgag tttatataca gctagagtcg aagtagtgat tgggagacca acccagtgga
     1921 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     1981 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2041 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2101 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2161 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     2221 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2281 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2341 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2401 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2461 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2521 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2581 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2641 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2701 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2761 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2821 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2881 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     2941 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     3001 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     3061 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccggtc
     3121 tcggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     3181 agtggcaccg agtcggtgct tttttttgca aaattttcca gatcgatttc ttcttcctct
     3241 gttcttcggc gttcaatttc tggggttttc tcttcgtttt ctgtaactga aacctaaaat
     3301 ttgacctaaa aaaaatctca aataatatga ttcagtggtt ttgtactttt cagttagttg
     3361 agttttgcag ttccgatgag ataaaccaat accatggtta tactagtgaa taaaagcatt
     3421 tgcgtttggt ttatcattgc gtttatacaa ggacagagat ccactgagct ggaatagctt
     3481 aaaaccatta tcagaacaaa ataaaccatt ttttgttaag aatcagagca tagtaaacaa
     3541 cagaaacaac ctaagagagg taacttgtcc aagaagatag ctaattatat ctattttata
     3601 aaagttatca tagtttgtaa gtcacaaaag atgcaaataa cagagaaact aggagacttg
     3661 agaatataca ttcttgtata tttgtattcg agattgtgaa aatttgacca taagtttaaa
     3721 ttcttaaaaa gatatatctg atctaggtga tggttataga ctgtaatttt accacatgtt
     3781 taatgatgga tagtgacaca catgacacat cgacaacact atagcatctt atttagatta
     3841 caacatgaaa tttttctgta atacatgtct ttgtacataa tttaaaagta attcctaaga
     3901 aatatattta tacaaggagt ttaaagaaaa catagcataa agttcaatga gtagtaaaaa
     3961 ccatatacag tatatagcat aaagttcaat gagtttatta caaaagcatt ggttcacttt
     4021 ctgtaacacg acgttaaacc ttcgtctcca ataggagcgc tactgattca acatgccaat
     4081 atatactaaa tacgtttcta cagtcaaatg ctttaacgtt tcatgattaa gtgactattt
     4141 accgtcaatc ctttcccatt cctcccacta atccaacttt ttaattactc ttaaatcacc
     4201 actaagctag taacgcctat catgaattag ctctactaaa tctagcaacc tttcaaattt
     4261 gcagtattgc aggtgtctct gtgtctttaa aatagttgcc ttatgatttc ttcggtttca
     4321 agatgatcaa atagttatag atttcatgct cacacatgct cattagatgt gtacatactt
     4381 tacttaccca aatctatttt ctcgcaaaga ttttgatggt aaagctgatt tggttctatt
     4441 gaactaaatc aaacgagttt cagactgagt gattctaatc cggcccatta gcccctaaac
     4501 agacccacta attacgcagc ttttaataga gtaattacac ctagtttacc cactaaacca
     4561 ctaagcacta attatctcac aatctaatga gcttccctcg taattacttg ggctttcact
     4621 ctaccattta tttgtaacag tcaagtctct actgtctcta tataaactct ctaaagttaa
     4681 cacacaattc tcatcacaaa caaatcaacc aaagcaactt ctactctttc ttctttcgac
     4741 cttatcaatc tgttgagaaa tctagatgga ttacaaggac cacgacgggg attacaagga
     4801 ccacgacatt gattacaagg atgatgatga caagatggct ccgaagaaga agaggaaggt
     4861 tggcatccac ggggtgccag ctgctgacaa gaagtactcg atcggcctcg atattgggac
     4921 taactctgtt ggctgggccg tgatcaccga cgagtacaag gtgccctcaa agaagttcaa
     4981 ggtcctgggc aacaccgatc ggcattccat caagaagaat ctcattggcg ctctcctgtt
     5041 cgacagcggc gagacggctg aggctacgcg gctcaagcgc accgcccgca ggcggtacac
     5101 gcgcaggaag aatcgcatct gctacctgca ggagattttc tccaacgaga tggcgaaggt
     5161 tgacgattct ttcttccaca ggctggagga gtcattcctc gtggaggagg ataagaagca
     5221 cgagcggcat ccaatcttcg gcaacattgt cgacgaggtt gcctaccacg agaagtaccc
     5281 tacgatctac catctgcgga agaagctcgt ggactccaca gataaggcgg acctccgcct
     5341 gatctacctc gctctggccc acatgattaa gttcaggggc catttcctga tcgaggggga
     5401 tctcaacccg gacaatagcg atgttgacaa gctgttcatc cagctcgtgc agacgtacaa
     5461 ccagctcttc gaggagaacc ccattaatgc gtcaggcgtc gacgcgaagg ctatcctgtc
     5521 cgctaggctc tcgaagtctc ggcgcctcga gaacctgatc gcccagctgc cgggcgagaa
     5581 gaagaacggc ctgttcggga atctcattgc gctcagcctg gggctcacgc ccaacttcaa
     5641 gtcgaatttc gatctcgctg aggacgccaa gctgcagctc tccaaggaca catacgacga
     5701 tgacctggat aacctcctgg cccagatcgg cgatcagtac gcggacctgt tcctcgctgc
     5761 caagaatctg tcggacgcca tcctcctgtc tgatattctc agggtgaaca ccgagattac
     5821 gaaggctccg ctctcagcct ccatgatcaa gcgctacgac gagcaccatc aggatctgac
     5881 cctcctgaag gcgctggtca ggcagcagct ccccgagaag tacaaggaga tcttcttcga
     5941 tcagtcgaag aacggctacg ctgggtacat tgacggcggg gcctctcagg aggagttcta
     6001 caagttcatc aagccgattc tggagaagat ggacggcacg gaggagctgc tggtgaagct
     6061 caatcgcgag gacctcctga ggaagcagcg gacattcgat aacggcagca tcccacacca
     6121 gattcatctc ggggagctgc acgctatcct gaggaggcag gaggacttct accctttcct
     6181 caaggataac cgcgagaaga tcgagaagat tctgactttc aggatcccgt actacgtcgg
     6241 cccactcgct aggggcaact cccgcttcgc ttggatgacc cgcaagtcag aggagacgat
     6301 cacgccgtgg aacttcgagg aggtggtcga caagggcgct agcgctcagt cgttcatcga
     6361 gaggatgacg aatttcgaca agaacctgcc aaatgagaag gtgctcccta agcactcgct
     6421 cctgtacgag tacttcacag tctacaacga gctgactaag gtgaagtatg tgaccgaggg
     6481 catgaggaag ccggctttcc tgtctgggga gcagaagaag gccatcgtgg acctcctgtt
     6541 caagaccaac cggaaggtca cggttaagca gctcaaggag gactacttca agaagattga
     6601 gtgcttcgat tcggtcgaga tctctggcgt tgaggaccgc ttcaacgcct ccctggggac
     6661 ctaccacgat ctcctgaaga tcattaagga taaggacttc ctggacaacg aggagaatga
     6721 ggatatcctc gaggacattg tgctgacact cactctgttc gaggaccggg agatgatcga
     6781 ggagcgcctg aagacttacg cccatctctt cgatgacaag gtcatgaagc agctcaagag
     6841 gaggaggtac accggctggg ggaggctgag caggaagctc atcaacggca ttcgggacaa
     6901 gcagtccggg aagacgatcc tcgacttcct gaagagcgat ggcttcgcga accgcaattt
     6961 catgcagctg attcacgatg acagcctcac attcaaggag gatatccaga aggctcaggt
     7021 gagcggccag ggggactcgc tgcacgagca tatcgcgaac ctcgctggct cgccagctat
     7081 caagaagggg attctgcaga ccgtgaaggt tgtggacgag ctggtgaagg tcatgggcag
     7141 gcacaagcct gagaacatcg tcattgagat ggcccgggag aatcagacca cgcagaaggg
     7201 ccagaagaac tcacgcgaga ggatgaagag gatcgaggag ggcattaagg agctggggtc
     7261 ccagatcctc aaggagcacc cggtggagaa cacgcagctg cagaatgaga agctctacct
     7321 gtactacctc cagaatggcc gcgatatgta tgtggaccag gagctggata ttaacaggct
     7381 cagcgattac gacgtcgatc atatcgttcc acagtcattc ctgaaggatg actccattga
     7441 caacaaggtc ctcaccaggt cggacaagaa ccggggcaag tctgataatg ttccttcaga
     7501 ggaggtcgtt aagaagatga agaactactg gcgccagctc ctgaatgcca agctgatcac
     7561 gcagcggaag ttcgataacc tcacaaaggc tgagaggggc gggctctctg agctggacaa
     7621 ggcgggcttc atcaagaggc agctggtcga gacacggcag atcactaagc acgttgcgca
     7681 gattctcgac tcacggatga acactaagta cgatgagaat gacaagctga tccgcgaggt
     7741 gaaggtcatc accctgaagt caaagctcgt ctccgacttc aggaaggatt tccagttcta
     7801 caaggttcgg gagatcaaca attaccacca tgcccatgac gcgtacctga acgcggtggt
     7861 cggcacagct ctgatcaaga agtacccaaa gctcgagagc gagttcgtgt acggggacta
     7921 caaggtttac gatgtgagga agatgatcgc caagtcggag caggagattg gcaaggctac
     7981 cgccaagtac ttcttctact ctaacattat gaatttcttc aagacagaga tcactctggc
     8041 caatggcgag atccggaagc gccccctcat cgagacgaac ggcgagacgg gggagatcgt
     8101 gtgggacaag ggcagggatt tcgcgaccgt caggaaggtt ctctccatgc cacaagtgaa
     8161 tatcgtcaag aagacagagg tccagactgg cgggttctct aaggagtcaa ttctgcctaa
     8221 gcggaacagc gacaagctca tcgcccgcaa gaaggactgg gatccgaaga agtacggcgg
     8281 gttcgacagc cccactgtgg cctactcggt cctggttgtg gcgaaggttg agaagggcaa
     8341 gtccaagaag ctcaagagcg tgaaggagct gctggggatc acgattatgg agcgctccag
     8401 cttcgagaag aacccgatcg atttcctgga ggcgaagggc tacaaggagg tgaagaagga
     8461 cctgatcatt aagctcccca agtactcact cttcgagctg gagaacggca ggaagcggat
     8521 gctggcttcc gctggcgagc tgcagaaggg gaacgagctg gctctgccgt ccaagtatgt
     8581 gaacttcctc tacctggcct cccactacga gaagctcaag ggcagccccg aggacaacga
     8641 gcagaagcag ctgttcgtcg agcagcacaa gcattacctc gacgagatca ttgagcagat
     8701 ttccgagttc tccaagcgcg tgatcctggc cgacgcgaat ctggataagg tcctctccgc
     8761 gtacaacaag caccgcgaca agccaatcag ggagcaggct gagaatatca ttcatctctt
     8821 caccctgacg aacctcggcg cccctgctgc tttcaagtac ttcgacacaa ctatcgatcg
     8881 caagaggtac acaagcacta aggaggtcct ggacgcgacc ctcatccacc agtcgattac
     8941 cggcctctac gagacgcgca tcgacctgtc tcagctcggg ggcgacaagc ggccagcggc
     9001 gacgaagaag gcggggcagg cgaagaagaa gaagtgagct cagagctttc gttcgtatca
     9061 tcggtttcga caacgttcgt caagttcaat gcatcagttt cattgcgcac acaccagaat
     9121 cctactgagt ttgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg
     9181 cttgtaattt actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga
     9241 tggagaagag ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt
     9301 tttttctctt atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt
     9361 tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat gaggagtaaa
     9421 acacttgtag ttgtaccatt atgcttattc actaggcaac aaatatattt tcagacctag
     9481 aaaagctgca aatgttactg aatacaagta tgtcctcttg tgttttagac atttatgaac
     9541 tttcctttat gtaattttcc agaatccttg tcagattcta atcattgctt tataattata
     9601 gttatactca tggatttgta gttgagtatg aaaatatttt ttaatgcatt ttatgacttg
     9661 ccaattgggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9721 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9781 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     9841 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga
     9901 gcagcttgcc aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac
     9961 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct
    10021 cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg
    10081 tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc
    10141 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
    10201 tccaaccacg tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg
    10261 tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc
    10321 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
    10381 tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa
    10441 aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
    10501 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
    10561 atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct
    10621 ctatataagg aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta
    10681 caaatctatc tctctcgagc tttcgcagat ctgtcgatcg accatgggga ttgaacaaga
    10741 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
    10801 acaacagaca atcggctgct ctgatgccgc cgtgtttcgg ctgtcagcgc aggggcgccc
    10861 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactccagg acgaggcagc
    10921 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
    10981 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
    11041 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
    11101 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
    11161 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
    11221 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
    11281 cgtgacacat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
    11341 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
    11401 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
    11461 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
    11521 agcgggactc tggggttcgg atcgatcctc tagctagagt cgatcgacaa gctcgagttt
    11581 ctccataata atgtgtgagt agttcccaga taagggaatt agggttccta tagggtttcg
    11641 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
    11701 tcaataaaat ttctaattcc taaaaccaaa atccagtact aaaatccaga tcccccgaat
    11761 taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag
    11821 cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg
    11881 gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag
    11941 cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac
    12001 ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg
    12061 taacgatgac agagcgttgc tgcctgtgat caccgcggtt tcaaaatcgg ctccgtcgat
    12121 actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg
    12181 ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt
    12241 atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac
    12301 cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc
    12361 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
    12421 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
    12481 aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc
    12541 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
    12601 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
    12661 cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga
    12721 ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc
    12781 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
    12841 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
    12901 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga
    12961 tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
    13021 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag
    13081 aatgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgctg agagatcccc
    13141 tcataatttc cccaaagcgt aaccatgtgt gaataaattt tgagctagta gggttgcagc
    13201 cacgagtaag tcttcccttg ttattgtgta gccagaatgc cgcaaaactt ccatgcctaa
    13261 gcgaactgtt gagagtacgt ttcgatttct gactgtgtta gcctggaagt gcttgtccca
    13321 accttgtttc tgagcatgaa cgcccgcaag ccaacatgtt agttgaagca tcagggcgat
    13381 tagcagcatg atatcaaaac gctctgagct gctcgttcgg ctatggcgta ggcctagtcc
    13441 gtaggcagga cttttcaagt ctcggaaggt ttcttcaatc tgcattcgct tcgaatagat
    13501 attaacaagt tgtttgggtg ttcgaatttc aacaggtaag ttagttgcta gaacccatgg
    13561 ctcctttgcc gacgctgagt agattttagg tgacgggtgg tgacaatgag tccgtgtcga
    13621 gcgctgattt tttcggcctt tagagcgaga tttatacaat agaatttggc atgagattgg
    13681 attgctttta gtcagcctct tatagcctaa agtctttgag tgactagatg acatatcatg
    13741 taagttgctg ataggtttcc agttttccgc tcctaggtct gcatattgta cttttcctct
    13801 tactcgactt aaccagtacc aacccagctt ctcaacggat ttataccatg gcactttaaa
    13861 gccagcatca ctgacaatga gcggtgtggt gttactcggt agaatgctcg caaggtcggc
    13921 tagaaattgg tcatgagctt tctttgaaca ttgctctgaa agcgggaacg ctttctcata
    13981 aagagtaaca gaacgaccgt gtagtgcgac tgaagctcgc aataccataa gtcgtttttg
    14041 ctcacgaata tcagaccagt caacaagtac aatgggcatc gtattgcccg aacagataaa
    14101 gctagcatgc caacggtata cagcgagtcg ctctttgtgg aggtgacgat tacctaacaa
    14161 tcggtcgatt cgtttgatgt tatgttttgt tctcgctttg gttggcaggt tacggccaag
    14221 ttcggtaaga gtgagagttt tacagtcaag taatgcgtgg caagccaacg ttaagctgtt
    14281 gagtcgtttt aagtgtaatt cggggcagaa ttggtaaaga gagtcgtgta aaatatcgag
    14341 ttcgcacatc ttgttgtctg attattgatt tttcgcgaaa ccatttgatc atatgacaag
    14401 atgtgtatcc accttaactt aatgattttt accaaaatca ttaggggatt catcagcact
    14461 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    14521 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    14581 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    14641 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    14701 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    14761 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    14821 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    14881 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    14941 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    15001 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    15061 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    15121 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    15181 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    15241 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
    15301 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
    15361 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
    15421 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
    15481 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
    15541 acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt
    15601 gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg
    15661 cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt
    15721 tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa
    15781 gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat
    15841 cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt
    15901 tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaacagac
    15961 cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg
    16021 aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag
    16081 cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc
    16141 gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct
    16201 tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac
    16261 ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct
    16321 tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc
    16381 gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg
    16441 tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta
    16501 accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga
    16561 acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt
    16621 cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat
    16681 cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct
    16741 cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt
    16801 ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg
    16861 gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc
    16921 gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg
    16981 cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac
    17041 ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc
    17101 gggcttgggg gttccagtgc cattgcaggg ccggcaggca acccagccgc ttacgcctgg
    17161 ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg
    17221 attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca
    17281 tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc
    17341 gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct
    17401 tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg
    17461 gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca
    17521 aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc
    17581 gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg
    17641 cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc
    17701 gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc
    17761 cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta
    17821 agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa
    17881 gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac
    17941 gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac
    18001 ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc
    18061 ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt
    18121 ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat
    18181 cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt
    18241 tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg
    18301 gcatcagaca aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc
    18361 tcgaacacgt acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt
    18421 tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg
    18481 ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct
    18541 cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc
    18601 caagcagtgc agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt
    18661 gcgcgatctg tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg
    18721 cggcctcgcg cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc
    18781 cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg
    18841 ccaggctacg caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc
    18901 gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt
    18961 ggtcaagcat cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa
    19021 acagcttggt gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt
    19081 cggtgctgac gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat
    19141 gtctccggtt ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac
    19201 gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt
    19261 tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt
    19321 ggatcaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac
    19381 gaacggataa accttttcac gcccttttaa atatccgatt attctaataa acgctctttt
    19441 ctcttag
//
