LOCUS       sequence-446478-       19133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, 
            bialaphos and red fluorescent seed selection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19133)
  TITLE     Takeuchi CRISPR/Cas9 Plasmids for multi-gene knockout in Arabidopsis
REFERENCE   2  (bases 1 to 19133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHEE-R-bar
FEATURES             Location/Qualifiers
     source          1..19133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             531..1238
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     944..963
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2181..2972
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        3124..3199
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4769..4834
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4841..4861
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4886..8986
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4886..8986
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             8899..8907
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8987..9034
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(9680..9696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9680..9696)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9693..9715)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9704..9720
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9728..9734,9735..9752,9753..9758))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9773..9794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9985..10661
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10598..10618
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             10706..11257
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    11264..11438
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11516..11540
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11965..12759
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14184..14772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14673..14692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14926..14943
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    14958..15098
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15024..15043)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15184..15206
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      15442..15636
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(15702..16775)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17204..17833)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagtttc ttgaaaccaa attaacatag
       61 ggtttttatt taaataaaag tttaaccttc tttttaaaaa attgttcata gtgtcatgtc
      121 agaacaagag ctacaaatca cacatagcat gcataagcgg agctatgatg agtggtattg
      181 ttttgttcgt cacttgtcac tcttttccaa cacataatcc cgacaacaac gtaagagcat
      241 ctctctctct ccacacacac tcatgcatgc atgcattctt acacgtgatt gccatgcaaa
      301 tctcctttct cacctataaa tacaaaccaa cccttcacta cactcttcac tcaaaccaaa
      361 acaagaaaac atacacaaat agcaaaacaa gggcgaattc tcaacacaac atatacaaaa
      421 caaacgaatc tcaagcaatc aagcattcta cttctattgc agcaatttaa atcatttctt
      481 ttaaagcaaa agcaattttc tgaaaatttt caccatttac gaacgatagc atggtgagca
      541 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      601 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      661 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      721 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      781 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      841 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      901 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
      961 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1021 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1081 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1141 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1201 gccgccactc caccggcggc atggacgagc tgtacaagaa ggacgagctg taagcgccgc
     1261 ccggctgcat cgctgaaatc accagtctct ctctacaaat ctatctctct ctataataat
     1321 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     1381 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     1441 ctaattccta aaaccaaaat ccagtggtac caagcttcga cttgccttcc gcacaataca
     1501 tcatttcttc ttagcttttt ttcttcttct tcgttcatac agtttttttt tgtttatcag
     1561 cttacatttt cttgaaccgt agctttcgtt ttcttctttt taactttcca ttcggagttt
     1621 ttgtatcttg tttcatagtt tgtcccagga ttagaatgat taggcatcga accttcaaga
     1681 atttgattga ataaaacatc ttcattctta agatatgaag ataatcttca aaaggcccct
     1741 gggaatctga aagaagagaa gcaggcccat ttatatggga aagaacaata gtatttctta
     1801 tataggccca tttaagttga aaacaatctt caaaagtccc acatcgctta gataagaaaa
     1861 cgaagctgag tttatataca gctagagtcg aagtagtgat tgggagacca acccagtgga
     1921 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     1981 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2041 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2101 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2161 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     2221 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2281 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2341 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2401 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2461 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2521 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2581 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2641 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2701 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2761 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2821 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2881 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     2941 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     3001 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     3061 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccggtc
     3121 tcggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     3181 agtggcaccg agtcggtgct tttttttgca aaattttcca gatcgatttc ttcttcctct
     3241 gttcttcggc gttcaatttc tggggttttc tcttcgtttt ctgtaactga aacctaaaat
     3301 ttgacctaaa aaaaatctca aataatatga ttcagtggtt ttgtactttt cagttagttg
     3361 agttttgcag ttccgatgag ataaaccaat accatggtta tactagtgaa taaaagcatt
     3421 tgcgtttggt ttatcattgc gtttatacaa ggacagagat ccactgagct ggaatagctt
     3481 aaaaccatta tcagaacaaa ataaaccatt ttttgttaag aatcagagca tagtaaacaa
     3541 cagaaacaac ctaagagagg taacttgtcc aagaagatag ctaattatat ctattttata
     3601 aaagttatca tagtttgtaa gtcacaaaag atgcaaataa cagagaaact aggagacttg
     3661 agaatataca ttcttgtata tttgtattcg agattgtgaa aatttgacca taagtttaaa
     3721 ttcttaaaaa gatatatctg atctaggtga tggttataga ctgtaatttt accacatgtt
     3781 taatgatgga tagtgacaca catgacacat cgacaacact atagcatctt atttagatta
     3841 caacatgaaa tttttctgta atacatgtct ttgtacataa tttaaaagta attcctaaga
     3901 aatatattta tacaaggagt ttaaagaaaa catagcataa agttcaatga gtagtaaaaa
     3961 ccatatacag tatatagcat aaagttcaat gagtttatta caaaagcatt ggttcacttt
     4021 ctgtaacacg acgttaaacc ttcgtctcca ataggagcgc tactgattca acatgccaat
     4081 atatactaaa tacgtttcta cagtcaaatg ctttaacgtt tcatgattaa gtgactattt
     4141 accgtcaatc ctttcccatt cctcccacta atccaacttt ttaattactc ttaaatcacc
     4201 actaagctag taacgcctat catgaattag ctctactaaa tctagcaacc tttcaaattt
     4261 gcagtattgc aggtgtctct gtgtctttaa aatagttgcc ttatgatttc ttcggtttca
     4321 agatgatcaa atagttatag atttcatgct cacacatgct cattagatgt gtacatactt
     4381 tacttaccca aatctatttt ctcgcaaaga ttttgatggt aaagctgatt tggttctatt
     4441 gaactaaatc aaacgagttt cagactgagt gattctaatc cggcccatta gcccctaaac
     4501 agacccacta attacgcagc ttttaataga gtaattacac ctagtttacc cactaaacca
     4561 ctaagcacta attatctcac aatctaatga gcttccctcg taattacttg ggctttcact
     4621 ctaccattta tttgtaacag tcaagtctct actgtctcta tataaactct ctaaagttaa
     4681 cacacaattc tcatcacaaa caaatcaacc aaagcaactt ctactctttc ttctttcgac
     4741 cttatcaatc tgttgagaaa tctagatgga ttacaaggac cacgacgggg attacaagga
     4801 ccacgacatt gattacaagg atgatgatga caagatggct ccgaagaaga agaggaaggt
     4861 tggcatccac ggggtgccag ctgctgacaa gaagtactcg atcggcctcg atattgggac
     4921 taactctgtt ggctgggccg tgatcaccga cgagtacaag gtgccctcaa agaagttcaa
     4981 ggtcctgggc aacaccgatc ggcattccat caagaagaat ctcattggcg ctctcctgtt
     5041 cgacagcggc gagacggctg aggctacgcg gctcaagcgc accgcccgca ggcggtacac
     5101 gcgcaggaag aatcgcatct gctacctgca ggagattttc tccaacgaga tggcgaaggt
     5161 tgacgattct ttcttccaca ggctggagga gtcattcctc gtggaggagg ataagaagca
     5221 cgagcggcat ccaatcttcg gcaacattgt cgacgaggtt gcctaccacg agaagtaccc
     5281 tacgatctac catctgcgga agaagctcgt ggactccaca gataaggcgg acctccgcct
     5341 gatctacctc gctctggccc acatgattaa gttcaggggc catttcctga tcgaggggga
     5401 tctcaacccg gacaatagcg atgttgacaa gctgttcatc cagctcgtgc agacgtacaa
     5461 ccagctcttc gaggagaacc ccattaatgc gtcaggcgtc gacgcgaagg ctatcctgtc
     5521 cgctaggctc tcgaagtctc ggcgcctcga gaacctgatc gcccagctgc cgggcgagaa
     5581 gaagaacggc ctgttcggga atctcattgc gctcagcctg gggctcacgc ccaacttcaa
     5641 gtcgaatttc gatctcgctg aggacgccaa gctgcagctc tccaaggaca catacgacga
     5701 tgacctggat aacctcctgg cccagatcgg cgatcagtac gcggacctgt tcctcgctgc
     5761 caagaatctg tcggacgcca tcctcctgtc tgatattctc agggtgaaca ccgagattac
     5821 gaaggctccg ctctcagcct ccatgatcaa gcgctacgac gagcaccatc aggatctgac
     5881 cctcctgaag gcgctggtca ggcagcagct ccccgagaag tacaaggaga tcttcttcga
     5941 tcagtcgaag aacggctacg ctgggtacat tgacggcggg gcctctcagg aggagttcta
     6001 caagttcatc aagccgattc tggagaagat ggacggcacg gaggagctgc tggtgaagct
     6061 caatcgcgag gacctcctga ggaagcagcg gacattcgat aacggcagca tcccacacca
     6121 gattcatctc ggggagctgc acgctatcct gaggaggcag gaggacttct accctttcct
     6181 caaggataac cgcgagaaga tcgagaagat tctgactttc aggatcccgt actacgtcgg
     6241 cccactcgct aggggcaact cccgcttcgc ttggatgacc cgcaagtcag aggagacgat
     6301 cacgccgtgg aacttcgagg aggtggtcga caagggcgct agcgctcagt cgttcatcga
     6361 gaggatgacg aatttcgaca agaacctgcc aaatgagaag gtgctcccta agcactcgct
     6421 cctgtacgag tacttcacag tctacaacga gctgactaag gtgaagtatg tgaccgaggg
     6481 catgaggaag ccggctttcc tgtctgggga gcagaagaag gccatcgtgg acctcctgtt
     6541 caagaccaac cggaaggtca cggttaagca gctcaaggag gactacttca agaagattga
     6601 gtgcttcgat tcggtcgaga tctctggcgt tgaggaccgc ttcaacgcct ccctggggac
     6661 ctaccacgat ctcctgaaga tcattaagga taaggacttc ctggacaacg aggagaatga
     6721 ggatatcctc gaggacattg tgctgacact cactctgttc gaggaccggg agatgatcga
     6781 ggagcgcctg aagacttacg cccatctctt cgatgacaag gtcatgaagc agctcaagag
     6841 gaggaggtac accggctggg ggaggctgag caggaagctc atcaacggca ttcgggacaa
     6901 gcagtccggg aagacgatcc tcgacttcct gaagagcgat ggcttcgcga accgcaattt
     6961 catgcagctg attcacgatg acagcctcac attcaaggag gatatccaga aggctcaggt
     7021 gagcggccag ggggactcgc tgcacgagca tatcgcgaac ctcgctggct cgccagctat
     7081 caagaagggg attctgcaga ccgtgaaggt tgtggacgag ctggtgaagg tcatgggcag
     7141 gcacaagcct gagaacatcg tcattgagat ggcccgggag aatcagacca cgcagaaggg
     7201 ccagaagaac tcacgcgaga ggatgaagag gatcgaggag ggcattaagg agctggggtc
     7261 ccagatcctc aaggagcacc cggtggagaa cacgcagctg cagaatgaga agctctacct
     7321 gtactacctc cagaatggcc gcgatatgta tgtggaccag gagctggata ttaacaggct
     7381 cagcgattac gacgtcgatc atatcgttcc acagtcattc ctgaaggatg actccattga
     7441 caacaaggtc ctcaccaggt cggacaagaa ccggggcaag tctgataatg ttccttcaga
     7501 ggaggtcgtt aagaagatga agaactactg gcgccagctc ctgaatgcca agctgatcac
     7561 gcagcggaag ttcgataacc tcacaaaggc tgagaggggc gggctctctg agctggacaa
     7621 ggcgggcttc atcaagaggc agctggtcga gacacggcag atcactaagc acgttgcgca
     7681 gattctcgac tcacggatga acactaagta cgatgagaat gacaagctga tccgcgaggt
     7741 gaaggtcatc accctgaagt caaagctcgt ctccgacttc aggaaggatt tccagttcta
     7801 caaggttcgg gagatcaaca attaccacca tgcccatgac gcgtacctga acgcggtggt
     7861 cggcacagct ctgatcaaga agtacccaaa gctcgagagc gagttcgtgt acggggacta
     7921 caaggtttac gatgtgagga agatgatcgc caagtcggag caggagattg gcaaggctac
     7981 cgccaagtac ttcttctact ctaacattat gaatttcttc aagacagaga tcactctggc
     8041 caatggcgag atccggaagc gccccctcat cgagacgaac ggcgagacgg gggagatcgt
     8101 gtgggacaag ggcagggatt tcgcgaccgt caggaaggtt ctctccatgc cacaagtgaa
     8161 tatcgtcaag aagacagagg tccagactgg cgggttctct aaggagtcaa ttctgcctaa
     8221 gcggaacagc gacaagctca tcgcccgcaa gaaggactgg gatccgaaga agtacggcgg
     8281 gttcgacagc cccactgtgg cctactcggt cctggttgtg gcgaaggttg agaagggcaa
     8341 gtccaagaag ctcaagagcg tgaaggagct gctggggatc acgattatgg agcgctccag
     8401 cttcgagaag aacccgatcg atttcctgga ggcgaagggc tacaaggagg tgaagaagga
     8461 cctgatcatt aagctcccca agtactcact cttcgagctg gagaacggca ggaagcggat
     8521 gctggcttcc gctggcgagc tgcagaaggg gaacgagctg gctctgccgt ccaagtatgt
     8581 gaacttcctc tacctggcct cccactacga gaagctcaag ggcagccccg aggacaacga
     8641 gcagaagcag ctgttcgtcg agcagcacaa gcattacctc gacgagatca ttgagcagat
     8701 ttccgagttc tccaagcgcg tgatcctggc cgacgcgaat ctggataagg tcctctccgc
     8761 gtacaacaag caccgcgaca agccaatcag ggagcaggct gagaatatca ttcatctctt
     8821 caccctgacg aacctcggcg cccctgctgc tttcaagtac ttcgacacaa ctatcgatcg
     8881 caagaggtac acaagcacta aggaggtcct ggacgcgacc ctcatccacc agtcgattac
     8941 cggcctctac gagacgcgca tcgacctgtc tcagctcggg ggcgacaagc ggccagcggc
     9001 gacgaagaag gcggggcagg cgaagaagaa gaagtgagct cagagctttc gttcgtatca
     9061 tcggtttcga caacgttcgt caagttcaat gcatcagttt cattgcgcac acaccagaat
     9121 cctactgagt ttgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg
     9181 cttgtaattt actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga
     9241 tggagaagag ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt
     9301 tttttctctt atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt
     9361 tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat gaggagtaaa
     9421 acacttgtag ttgtaccatt atgcttattc actaggcaac aaatatattt tcagacctag
     9481 aaaagctgca aatgttactg aatacaagta tgtcctcttg tgttttagac atttatgaac
     9541 tttcctttat gtaattttcc agaatccttg tcagattcta atcattgctt tataattata
     9601 gttatactca tggatttgta gttgagtatg aaaatatttt ttaatgcatt ttatgacttg
     9661 ccaattgggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9721 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9781 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     9841 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga
     9901 gcagcttgcc aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac
     9961 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct
    10021 cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg
    10081 tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc
    10141 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
    10201 tccaaccacg tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg
    10261 tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc
    10321 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
    10381 tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa
    10441 aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
    10501 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
    10561 atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct
    10621 ctatataagg aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta
    10681 caaatctatc tctctcgagt ctaccatgag cccagaacga cgcccggccg acatccgccg
    10741 tgccaccgag gcggacatgc cggcggtctg caccatcgtc aaccactaca tcgagacaag
    10801 cacggtcaac ttccgtaccg agccgcagga accgcaggag tggacggacg acctcgtccg
    10861 tctgcgggag cgctatccct ggctcgtcgc cgaggtggac ggcgaggtcg ccggcatcgc
    10921 ctacgcgggc ccctggaagg cacgcaacgc ctacgactgg acggccgagt cgaccgtgta
    10981 cgtctccccc cgccaccagc ggacgggact gggctccacg ctctacaccc acctgctgaa
    11041 gtccctggag gcacagggct tcaagagcgt ggtcgctgtc atcgggctgc ccaacgaccc
    11101 gagcgtgcgc atgcacgagg cgctcggata tgccccccgc ggcatgctgc gggcggccgg
    11161 cttcaagcac gggaactggc atgacgtggg tttctggcag ctggacttca gcctgccggt
    11221 accgccccgt ccggtcctgc ccgtcaccga gatttgactc gagtttctcc ataataatgt
    11281 gtgagtagtt cccagataag ggaattaggg ttcctatagg gtttcgctca tgtgttgagc
    11341 atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct
    11401 aattcctaaa accaaaatcc agtactaaaa tccagatccc ccgaattaat tcggcgttaa
    11461 ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta
    11521 caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa
    11581 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
    11641 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
    11701 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
    11761 cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc gtcgatacta tgttatacgc
    11821 caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag
    11881 gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg
    11941 tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa
    12001 ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat
    12061 aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg
    12121 accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct
    12181 gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag
    12241 gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc
    12301 gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat
    12361 acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc
    12421 gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat
    12481 gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga
    12541 gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc
    12601 ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc
    12661 ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag
    12721 aaaataaaat attatatttt actggatgaa ttgttttagt acctagaatg catgaccaaa
    12781 atcccttaac gtgagttttc gttccactga gcgctgagag atcccctcat aatttcccca
    12841 aagcgtaacc atgtgtgaat aaattttgag ctagtagggt tgcagccacg agtaagtctt
    12901 cccttgttat tgtgtagcca gaatgccgca aaacttccat gcctaagcga actgttgaga
    12961 gtacgtttcg atttctgact gtgttagcct ggaagtgctt gtcccaacct tgtttctgag
    13021 catgaacgcc cgcaagccaa catgttagtt gaagcatcag ggcgattagc agcatgatat
    13081 caaaacgctc tgagctgctc gttcggctat ggcgtaggcc tagtccgtag gcaggacttt
    13141 tcaagtctcg gaaggtttct tcaatctgca ttcgcttcga atagatatta acaagttgtt
    13201 tgggtgttcg aatttcaaca ggtaagttag ttgctagaac ccatggctcc tttgccgacg
    13261 ctgagtagat tttaggtgac gggtggtgac aatgagtccg tgtcgagcgc tgattttttc
    13321 ggcctttaga gcgagattta tacaatagaa tttggcatga gattggattg cttttagtca
    13381 gcctcttata gcctaaagtc tttgagtgac tagatgacat atcatgtaag ttgctgatag
    13441 gtttccagtt ttccgctcct aggtctgcat attgtacttt tcctcttact cgacttaacc
    13501 agtaccaacc cagcttctca acggatttat accatggcac tttaaagcca gcatcactga
    13561 caatgagcgg tgtggtgtta ctcggtagaa tgctcgcaag gtcggctaga aattggtcat
    13621 gagctttctt tgaacattgc tctgaaagcg ggaacgcttt ctcataaaga gtaacagaac
    13681 gaccgtgtag tgcgactgaa gctcgcaata ccataagtcg tttttgctca cgaatatcag
    13741 accagtcaac aagtacaatg ggcatcgtat tgcccgaaca gataaagcta gcatgccaac
    13801 ggtatacagc gagtcgctct ttgtggaggt gacgattacc taacaatcgg tcgattcgtt
    13861 tgatgttatg ttttgttctc gctttggttg gcaggttacg gccaagttcg gtaagagtga
    13921 gagttttaca gtcaagtaat gcgtggcaag ccaacgttaa gctgttgagt cgttttaagt
    13981 gtaattcggg gcagaattgg taaagagagt cgtgtaaaat atcgagttcg cacatcttgt
    14041 tgtctgatta ttgatttttc gcgaaaccat ttgatcatat gacaagatgt gtatccacct
    14101 taacttaatg atttttacca aaatcattag gggattcatc agcactgagc gtcagacccc
    14161 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    14221 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    14281 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
    14341 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    14401 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    14461 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    14521 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    14581 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    14641 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    14701 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    14761 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    14821 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    14881 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    14941 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
    15001 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
    15061 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
    15121 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
    15181 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg
    15241 gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt
    15301 agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc
    15361 gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg
    15421 gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt
    15481 tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg
    15541 tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg
    15601 tacggctttg ggttcccaat gtacgtgcta tccacaggaa acagaccttt tcgacctttt
    15661 tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct
    15721 tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc
    15781 tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag
    15841 aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg
    15901 gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga
    15961 tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc
    16021 tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc
    16081 tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg
    16141 ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt
    16201 tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc
    16261 gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc
    16321 atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc
    16381 atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc
    16441 tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga
    16501 ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc
    16561 ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg
    16621 attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc
    16681 cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg
    16741 atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc
    16801 cagtgccatt gcagggccgg caggcaaccc agccgcttac gcctggccaa ccgcccgttc
    16861 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc
    16921 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc
    16981 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc
    17041 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg
    17101 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt
    17161 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt
    17221 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa
    17281 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact
    17341 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc
    17401 ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc
    17461 gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca
    17521 ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg
    17581 gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg
    17641 tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc
    17701 gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg
    17761 cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag
    17821 cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc
    17881 agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg
    17941 ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc
    18001 ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc
    18061 ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc
    18121 ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg
    18181 gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact
    18241 tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc
    18301 gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc
    18361 ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg
    18421 ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc
    18481 aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg
    18541 cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc
    18601 aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg
    18661 gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag
    18721 ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg
    18781 gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag
    18841 tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc
    18901 agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc
    18961 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt
    19021 tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
    19081 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct tag
//
