LOCUS       sequence-446479-       19981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, 
            hygromycin and red fluorescent seed selection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19981)
  TITLE     Takeuchi CRISPR/Cas9 Plasmids for multi-gene knockout in Arabidopsis
REFERENCE   2  (bases 1 to 19981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHEE-R-hyg5A
FEATURES             Location/Qualifiers
     source          1..19981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             531..1238
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     944..963
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2181..2972
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        3124..3199
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             5076..5141
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5148..5168
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5193..9293
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5193..9293
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             9206..9214
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9294..9341
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(9998..10014)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9998..10014)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10011..10033)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10022..10038
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10046..10052,10053..10070,10071..10076))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10091..10112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10303..10979
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10916..10936
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11046..12071
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    12112..12286
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12364..12388
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12813..13607
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      15032..15620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15521..15540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15774..15791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15806..15946
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15872..15891)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     16032..16054
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      16290..16484
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16550..17623)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(18052..18681)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagtttc ttgaaaccaa attaacatag
       61 ggtttttatt taaataaaag tttaaccttc tttttaaaaa attgttcata gtgtcatgtc
      121 agaacaagag ctacaaatca cacatagcat gcataagcgg agctatgatg agtggtattg
      181 ttttgttcgt cacttgtcac tcttttccaa cacataatcc cgacaacaac gtaagagcat
      241 ctctctctct ccacacacac tcatgcatgc atgcattctt acacgtgatt gccatgcaaa
      301 tctcctttct cacctataaa tacaaaccaa cccttcacta cactcttcac tcaaaccaaa
      361 acaagaaaac atacacaaat agcaaaacaa gggcgaattc tcaacacaac atatacaaaa
      421 caaacgaatc tcaagcaatc aagcattcta cttctattgc agcaatttaa atcatttctt
      481 ttaaagcaaa agcaattttc tgaaaatttt caccatttac gaacgatagc atggtgagca
      541 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      601 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      661 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      721 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      781 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      841 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      901 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
      961 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1021 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1081 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1141 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1201 gccgccactc caccggcggc atggacgagc tgtacaagaa ggacgagctg taagcgccgc
     1261 ccggctgcat cgctgaaatc accagtctct ctctacaaat ctatctctct ctataataat
     1321 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     1381 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     1441 ctaattccta aaaccaaaat ccagtggtac caagcttcga cttgccttcc gcacaataca
     1501 tcatttcttc ttagcttttt ttcttcttct tcgttcatac agtttttttt tgtttatcag
     1561 cttacatttt cttgaaccgt agctttcgtt ttcttctttt taactttcca ttcggagttt
     1621 ttgtatcttg tttcatagtt tgtcccagga ttagaatgat taggcatcga accttcaaga
     1681 atttgattga ataaaacatc ttcattctta agatatgaag ataatcttca aaaggcccct
     1741 gggaatctga aagaagagaa gcaggcccat ttatatggga aagaacaata gtatttctta
     1801 tataggccca tttaagttga aaacaatctt caaaagtccc acatcgctta gataagaaaa
     1861 cgaagctgag tttatataca gctagagtcg aagtagtgat tgggagacca acccagtgga
     1921 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     1981 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2041 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2101 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2161 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     2221 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2281 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2341 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2401 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2461 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2521 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2581 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2641 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2701 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2761 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2821 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2881 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     2941 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     3001 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     3061 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccggtc
     3121 tcggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     3181 agtggcaccg agtcggtgct tttttttgca aaattttcca gatcgatttc ttcttcctct
     3241 gttcttcggc gttcaatttc tggggttttc tcttcgtttt ctgtaactga aacctaaaat
     3301 ttgacctaaa aaaaatctca aataatatga ttcagtggtt ttgtactttt cagttagttg
     3361 agttttgcag ttccgatgag ataaaccaat accatggtta tactagtctc aacttttgat
     3421 tcgctatttg cagtgcacct gtggcgttca tcacatcttt tgtgacactg tttgcactgg
     3481 tcattgctat tacaaaggac cttcctgatg ttgaaggaga tcgaaagtaa gtaactgcac
     3541 gcataaccat tttctttccg ctctttggct caatccattt gacagtcaaa gacaatgttt
     3601 aaccagctcc gtttgatata ttgtctttat gtgtttgttc aagcatgttt agttaatcat
     3661 gcctttgatt gatcttgaat aggttccaaa tatcaaccct ggcaacaaaa cttggagtga
     3721 gaaacattgc attcctcggt tctggacttc tgctagtaaa ttatgtttca gccatatcac
     3781 tagctttcta catgcctcag gtgaattcat ctatttccgt cttaactatt tcggttaatt
     3841 aaagcacgaa caccattact gcatgtagaa gcttgataaa ctatcgccac caatttattt
     3901 ttgttgcgat attgttactt tcctcagtat gcagctttga aaagaccaac cctcttatcc
     3961 tttaacaatg aacaggtttt tagaggtagc ttgatgattc ctgcacatgt gatcttggct
     4021 tcaggcttaa ttttccaggt aaagcattat gagatactct tatatctctt acatactttt
     4081 gagataatgc acaagaactt cataactata tgctttagtt tctgcatttg acactgccaa
     4141 attcattaat ctctaatatc tttgttgttg atctttggta gacatgggta ctagaaaaag
     4201 caaactacac caaggtaaaa tacttttgta caaacataaa ctcgttatca cggaacatca
     4261 atggagtgta tatctaacgg agtgtagaaa catttgatta ttgcaggaag ctatctcagg
     4321 atattatcgg tttatatgga atctcttcta cgcagagtat ctgttattcc ccttcctcta
     4381 gctttcaatt tcatggtgag gatatgcagt tttctttgta tatcattctt cttcttcttt
     4441 gtagcttgga gtcaaaatcg gttccttcat gtacatacat caaggatatg tccttctgaa
     4501 tttttatatc ttgcaataaa aatgcttgta ccaattgaaa caccagcttt ttgagttcta
     4561 tgatcactga cttggttcta accaaaaaaa aaaaaatgtt taatttacat atctaaaagt
     4621 aggtttaggg aaacctaaac agtaaaatat ttgtatatta ttcgaatttc actcatcata
     4681 aaaacttaaa ttgcaccata aaattttgtt ttactattaa tgatgtaatt tgtgtaactt
     4741 aagataaaaa taatattccg taagttaacc ggctaaaacc acgtataaac cagggaacct
     4801 gttaaaccgg ttctttactg gataaagaaa tgaaagccca tgtagacagc tccattagag
     4861 cccaaaccct aaatttctca tctatataaa aggagtgaca ttagggtttt tgttcgtcct
     4921 cttaaagctt ctcgttttct ctgccgtctc tctcattcgc gcgacgcaaa cgatcttcag
     4981 gtgatcttct ttctccaaat cctctctcat aactctgatt tcgtacttgt gtatttgagc
     5041 tcacgctctg tttctctcac cacagcctct agatggatta caaggaccac gacggggatt
     5101 acaaggacca cgacattgat tacaaggatg atgatgacaa gatggctccg aagaagaaga
     5161 ggaaggttgg catccacggg gtgccagctg ctgacaagaa gtactcgatc ggcctcgata
     5221 ttgggactaa ctctgttggc tgggccgtga tcaccgacga gtacaaggtg ccctcaaaga
     5281 agttcaaggt cctgggcaac accgatcggc attccatcaa gaagaatctc attggcgctc
     5341 tcctgttcga cagcggcgag acggctgagg ctacgcggct caagcgcacc gcccgcaggc
     5401 ggtacacgcg caggaagaat cgcatctgct acctgcagga gattttctcc aacgagatgg
     5461 cgaaggttga cgattctttc ttccacaggc tggaggagtc attcctcgtg gaggaggata
     5521 agaagcacga gcggcatcca atcttcggca acattgtcga cgaggttgcc taccacgaga
     5581 agtaccctac gatctaccat ctgcggaaga agctcgtgga ctccacagat aaggcggacc
     5641 tccgcctgat ctacctcgct ctggcccaca tgattaagtt caggggccat ttcctgatcg
     5701 agggggatct caacccggac aatagcgatg ttgacaagct gttcatccag ctcgtgcaga
     5761 cgtacaacca gctcttcgag gagaacccca ttaatgcgtc aggcgtcgac gcgaaggcta
     5821 tcctgtccgc taggctctcg aagtctcggc gcctcgagaa cctgatcgcc cagctgccgg
     5881 gcgagaagaa gaacggcctg ttcgggaatc tcattgcgct cagcctgggg ctcacgccca
     5941 acttcaagtc gaatttcgat ctcgctgagg acgccaagct gcagctctcc aaggacacat
     6001 acgacgatga cctggataac ctcctggccc agatcggcga tcagtacgcg gacctgttcc
     6061 tcgctgccaa gaatctgtcg gacgccatcc tcctgtctga tattctcagg gtgaacaccg
     6121 agattacgaa ggctccgctc tcagcctcca tgatcaagcg ctacgacgag caccatcagg
     6181 atctgaccct cctgaaggcg ctggtcaggc agcagctccc cgagaagtac aaggagatct
     6241 tcttcgatca gtcgaagaac ggctacgctg ggtacattga cggcggggcc tctcaggagg
     6301 agttctacaa gttcatcaag ccgattctgg agaagatgga cggcacggag gagctgctgg
     6361 tgaagctcaa tcgcgaggac ctcctgagga agcagcggac attcgataac ggcagcatcc
     6421 cacaccagat tcatctcggg gagctgcacg ctatcctgag gaggcaggag gacttctacc
     6481 ctttcctcaa ggataaccgc gagaagatcg agaagattct gactttcagg atcccgtact
     6541 acgtcggccc actcgctagg ggcaactccc gcttcgcttg gatgacccgc aagtcagagg
     6601 agacgatcac gccgtggaac ttcgaggagg tggtcgacaa gggcgctagc gctcagtcgt
     6661 tcatcgagag gatgacgaat ttcgacaaga acctgccaaa tgagaaggtg ctccctaagc
     6721 actcgctcct gtacgagtac ttcacagtct acaacgagct gactaaggtg aagtatgtga
     6781 ccgagggcat gaggaagccg gctttcctgt ctggggagca gaagaaggcc atcgtggacc
     6841 tcctgttcaa gaccaaccgg aaggtcacgg ttaagcagct caaggaggac tacttcaaga
     6901 agattgagtg cttcgattcg gtcgagatct ctggcgttga ggaccgcttc aacgcctccc
     6961 tggggaccta ccacgatctc ctgaagatca ttaaggataa ggacttcctg gacaacgagg
     7021 agaatgagga tatcctcgag gacattgtgc tgacactcac tctgttcgag gaccgggaga
     7081 tgatcgagga gcgcctgaag acttacgccc atctcttcga tgacaaggtc atgaagcagc
     7141 tcaagaggag gaggtacacc ggctggggga ggctgagcag gaagctcatc aacggcattc
     7201 gggacaagca gtccgggaag acgatcctcg acttcctgaa gagcgatggc ttcgcgaacc
     7261 gcaatttcat gcagctgatt cacgatgaca gcctcacatt caaggaggat atccagaagg
     7321 ctcaggtgag cggccagggg gactcgctgc acgagcatat cgcgaacctc gctggctcgc
     7381 cagctatcaa gaaggggatt ctgcagaccg tgaaggttgt ggacgagctg gtgaaggtca
     7441 tgggcaggca caagcctgag aacatcgtca ttgagatggc ccgggagaat cagaccacgc
     7501 agaagggcca gaagaactca cgcgagagga tgaagaggat cgaggagggc attaaggagc
     7561 tggggtccca gatcctcaag gagcacccgg tggagaacac gcagctgcag aatgagaagc
     7621 tctacctgta ctacctccag aatggccgcg atatgtatgt ggaccaggag ctggatatta
     7681 acaggctcag cgattacgac gtcgatcata tcgttccaca gtcattcctg aaggatgact
     7741 ccattgacaa caaggtcctc accaggtcgg acaagaaccg gggcaagtct gataatgttc
     7801 cttcagagga ggtcgttaag aagatgaaga actactggcg ccagctcctg aatgccaagc
     7861 tgatcacgca gcggaagttc gataacctca caaaggctga gaggggcggg ctctctgagc
     7921 tggacaaggc gggcttcatc aagaggcagc tggtcgagac acggcagatc actaagcacg
     7981 ttgcgcagat tctcgactca cggatgaaca ctaagtacga tgagaatgac aagctgatcc
     8041 gcgaggtgaa ggtcatcacc ctgaagtcaa agctcgtctc cgacttcagg aaggatttcc
     8101 agttctacaa ggttcgggag atcaacaatt accaccatgc ccatgacgcg tacctgaacg
     8161 cggtggtcgg cacagctctg atcaagaagt acccaaagct cgagagcgag ttcgtgtacg
     8221 gggactacaa ggtttacgat gtgaggaaga tgatcgccaa gtcggagcag gagattggca
     8281 aggctaccgc caagtacttc ttctactcta acattatgaa tttcttcaag acagagatca
     8341 ctctggccaa tggcgagatc cggaagcgcc ccctcatcga gacgaacggc gagacggggg
     8401 agatcgtgtg ggacaagggc agggatttcg cgaccgtcag gaaggttctc tccatgccac
     8461 aagtgaatat cgtcaagaag acagaggtcc agactggcgg gttctctaag gagtcaattc
     8521 tgcctaagcg gaacagcgac aagctcatcg cccgcaagaa ggactgggat ccgaagaagt
     8581 acggcgggtt cgacagcccc actgtggcct actcggtcct ggttgtggcg aaggttgaga
     8641 agggcaagtc caagaagctc aagagcgtga aggagctgct ggggatcacg attatggagc
     8701 gctccagctt cgagaagaac ccgatcgatt tcctggaggc gaagggctac aaggaggtga
     8761 agaaggacct gatcattaag ctccccaagt actcactctt cgagctggag aacggcagga
     8821 agcggatgct ggcttccgct ggcgagctgc agaaggggaa cgagctggct ctgccgtcca
     8881 agtatgtgaa cttcctctac ctggcctccc actacgagaa gctcaagggc agccccgagg
     8941 acaacgagca gaagcagctg ttcgtcgagc agcacaagca ttacctcgac gagatcattg
     9001 agcagatttc cgagttctcc aagcgcgtga tcctggccga cgcgaatctg gataaggtcc
     9061 tctccgcgta caacaagcac cgcgacaagc caatcaggga gcaggctgag aatatcattc
     9121 atctcttcac cctgacgaac ctcggcgccc ctgctgcttt caagtacttc gacacaacta
     9181 tcgatcgcaa gaggtacaca agcactaagg aggtcctgga cgcgaccctc atccaccagt
     9241 cgattaccgg cctctacgag acgcgcatcg acctgtctca gctcgggggc gacaagcggc
     9301 cagcggcgac gaagaaggcg gggcaggcga agaagaagaa gtgagctcag agctttcgtt
     9361 cgtatcatcg gtttcgacaa cgttcgtcaa gttcaatgca tcagtttcat tgcgcacaca
     9421 ccagaatcct actgagtttg agtattatgg cattgggaaa actgtttttc ttgtaccatt
     9481 tgttgtgctt gtaatttact gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg
     9541 aaatggatgg agaagagtta atgaatgata tggtcctttt gttcattctc aaattaatat
     9601 tatttgtttt ttctcttatt tgttgtgtgt tgaatttgaa attataagag atatgcaaac
     9661 attttgtttt gagtaaaaat gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag
     9721 gagtaaaaca cttgtagttg taccattatg cttattcact aggcaacaaa tatattttca
     9781 gacctagaaa agctgcaaat gttactgaat acaagtatgt cctcttgtgt tttagacatt
     9841 tatgaacttt cctttatgta attttccaga atccttgtca gattctaatc attgctttat
     9901 aattatagtt atactcatgg atttgtagtt gagtatgaaa atatttttta atgcatttta
     9961 tgacttgcca attgattgac aacgaattcg taatcatgtc atagctgttt cctgtgtgaa
    10021 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    10081 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    10141 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    10201 gtttgcgtat tggctagagc agcttgccaa catggtggag cacgacactc tcgtctactc
    10261 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
    10321 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
    10381 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
    10441 cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
    10501 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg
    10561 tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa
    10621 gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc
    10681 cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc
    10741 atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag
    10801 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
    10861 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
    10921 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa
    10981 atcaccagtc tctctctaca aatctatctc tctcgagctt tcgcagatcc ggggggcaat
    11041 gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
    11101 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
    11161 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
    11221 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
    11281 acattgggga gtttagcgag agcctgacct attgcatctc ccgccgttca cagggtgtca
    11341 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct acaaccggtc gcggaggcta
    11401 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
    11461 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
    11521 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
    11581 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
    11641 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
    11701 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
    11761 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
    11821 cgccacgact ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
    11881 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
    11941 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
    12001 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
    12061 caaagaaata gagtagatgc cgaccgggat ctgtcgatcg acaagctcga gtttctccat
    12121 aataatgtgt gagtagttcc cagataaggg aattagggtt cctatagggt ttcgctcatg
    12181 tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata
    12241 aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatccccc gaattaattc
    12301 ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
    12361 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
    12421 cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag
    12481 agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac
    12541 taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga
    12601 tgacagagcg ttgctgcctg tgatcaccgc ggtttcaaaa tcggctccgt cgatactatg
    12661 ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag
    12721 aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt
    12781 aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat
    12841 tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta
    12901 aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt
    12961 ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa
    13021 agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca
    13081 tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa
    13141 agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt
    13201 gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg
    13261 atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg
    13321 agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg
    13381 acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg
    13441 ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg
    13501 aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg
    13561 attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac ctagaatgca
    13621 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gctgagagat cccctcataa
    13681 tttccccaaa gcgtaaccat gtgtgaataa attttgagct agtagggttg cagccacgag
    13741 taagtcttcc cttgttattg tgtagccaga atgccgcaaa acttccatgc ctaagcgaac
    13801 tgttgagagt acgtttcgat ttctgactgt gttagcctgg aagtgcttgt cccaaccttg
    13861 tttctgagca tgaacgcccg caagccaaca tgttagttga agcatcaggg cgattagcag
    13921 catgatatca aaacgctctg agctgctcgt tcggctatgg cgtaggccta gtccgtaggc
    13981 aggacttttc aagtctcgga aggtttcttc aatctgcatt cgcttcgaat agatattaac
    14041 aagttgtttg ggtgttcgaa tttcaacagg taagttagtt gctagaaccc atggctcctt
    14101 tgccgacgct gagtagattt taggtgacgg gtggtgacaa tgagtccgtg tcgagcgctg
    14161 attttttcgg cctttagagc gagatttata caatagaatt tggcatgaga ttggattgct
    14221 tttagtcagc ctcttatagc ctaaagtctt tgagtgacta gatgacatat catgtaagtt
    14281 gctgataggt ttccagtttt ccgctcctag gtctgcatat tgtacttttc ctcttactcg
    14341 acttaaccag taccaaccca gcttctcaac ggatttatac catggcactt taaagccagc
    14401 atcactgaca atgagcggtg tggtgttact cggtagaatg ctcgcaaggt cggctagaaa
    14461 ttggtcatga gctttctttg aacattgctc tgaaagcggg aacgctttct cataaagagt
    14521 aacagaacga ccgtgtagtg cgactgaagc tcgcaatacc ataagtcgtt tttgctcacg
    14581 aatatcagac cagtcaacaa gtacaatggg catcgtattg cccgaacaga taaagctagc
    14641 atgccaacgg tatacagcga gtcgctcttt gtggaggtga cgattaccta acaatcggtc
    14701 gattcgtttg atgttatgtt ttgttctcgc tttggttggc aggttacggc caagttcggt
    14761 aagagtgaga gttttacagt caagtaatgc gtggcaagcc aacgttaagc tgttgagtcg
    14821 ttttaagtgt aattcggggc agaattggta aagagagtcg tgtaaaatat cgagttcgca
    14881 catcttgttg tctgattatt gatttttcgc gaaaccattt gatcatatga caagatgtgt
    14941 atccacctta acttaatgat ttttaccaaa atcattaggg gattcatcag cactgagcgt
    15001 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    15061 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    15121 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
    15181 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    15241 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    15301 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    15361 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    15421 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    15481 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    15541 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    15601 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    15661 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    15721 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    15781 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
    15841 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
    15901 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
    15961 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
    16021 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
    16081 gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc
    16141 gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag
    16201 gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca
    16261 gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt
    16321 taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca
    16381 cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt
    16441 tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaac agaccttttc
    16501 gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg
    16561 cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc
    16621 ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg
    16681 tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca
    16741 accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga
    16801 tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt
    16861 ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg
    16921 tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca
    16981 ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa
    17041 tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga
    17101 gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt
    17161 atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca
    17221 cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc
    17281 ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga
    17341 tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct
    17401 cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt
    17461 ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga
    17521 tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg
    17581 ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt
    17641 gggggttcca gtgccattgc agggccggca ggcaacccag ccgcttacgc ctggccaacc
    17701 gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc
    17761 catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact
    17821 ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg
    17881 cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg
    17941 ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc
    18001 cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag
    18061 ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc
    18121 tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat
    18181 acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat
    18241 gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac
    18301 ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct
    18361 tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc
    18421 cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg
    18481 gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc
    18541 ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag
    18601 ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt
    18661 aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc
    18721 atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga
    18781 cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca
    18841 gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac
    18901 acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc
    18961 tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca
    19021 gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg
    19081 gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca
    19141 gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga
    19201 tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct
    19261 cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga
    19321 acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc
    19381 tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc
    19441 tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa
    19501 gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct
    19561 tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc
    19621 tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc
    19681 ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg
    19741 aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag
    19801 aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca
    19861 aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg
    19921 ataaaccttt tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta
    19981 g
//
