LOCUS       sequence-446481-       19440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, 
            bialaphos and red fluorescent seed selection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19440)
  TITLE     Takeuchi CRISPR/Cas9 Plasmids for multi-gene knockout in Arabidopsis
REFERENCE   2  (bases 1 to 19440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHEE-R-bar5A
FEATURES             Location/Qualifiers
     source          1..19440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             531..1238
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     944..963
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2181..2972
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        3124..3199
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             5076..5141
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5148..5168
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5193..9293
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5193..9293
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             9206..9214
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9294..9341
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(9987..10003)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9987..10003)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10000..10022)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10011..10027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10035..10041,10042..10059,10060..10065))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10080..10101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10292..10968
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10905..10925
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11013..11564
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    11571..11745
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11823..11847
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12272..13066
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14491..15079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14980..14999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15233..15250
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15265..15405
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15331..15350)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15491..15513
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      15749..15943
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16009..17082)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17511..18140)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagtttc ttgaaaccaa attaacatag
       61 ggtttttatt taaataaaag tttaaccttc tttttaaaaa attgttcata gtgtcatgtc
      121 agaacaagag ctacaaatca cacatagcat gcataagcgg agctatgatg agtggtattg
      181 ttttgttcgt cacttgtcac tcttttccaa cacataatcc cgacaacaac gtaagagcat
      241 ctctctctct ccacacacac tcatgcatgc atgcattctt acacgtgatt gccatgcaaa
      301 tctcctttct cacctataaa tacaaaccaa cccttcacta cactcttcac tcaaaccaaa
      361 acaagaaaac atacacaaat agcaaaacaa gggcgaattc tcaacacaac atatacaaaa
      421 caaacgaatc tcaagcaatc aagcattcta cttctattgc agcaatttaa atcatttctt
      481 ttaaagcaaa agcaattttc tgaaaatttt caccatttac gaacgatagc atggtgagca
      541 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      601 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      661 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      721 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      781 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      841 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      901 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
      961 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1021 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1081 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1141 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1201 gccgccactc caccggcggc atggacgagc tgtacaagaa ggacgagctg taagcgccgc
     1261 ccggctgcat cgctgaaatc accagtctct ctctacaaat ctatctctct ctataataat
     1321 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     1381 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     1441 ctaattccta aaaccaaaat ccagtggtac caagcttcga cttgccttcc gcacaataca
     1501 tcatttcttc ttagcttttt ttcttcttct tcgttcatac agtttttttt tgtttatcag
     1561 cttacatttt cttgaaccgt agctttcgtt ttcttctttt taactttcca ttcggagttt
     1621 ttgtatcttg tttcatagtt tgtcccagga ttagaatgat taggcatcga accttcaaga
     1681 atttgattga ataaaacatc ttcattctta agatatgaag ataatcttca aaaggcccct
     1741 gggaatctga aagaagagaa gcaggcccat ttatatggga aagaacaata gtatttctta
     1801 tataggccca tttaagttga aaacaatctt caaaagtccc acatcgctta gataagaaaa
     1861 cgaagctgag tttatataca gctagagtcg aagtagtgat tgggagacca acccagtgga
     1921 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     1981 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2041 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2101 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2161 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     2221 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2281 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2341 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2401 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2461 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2521 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2581 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2641 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2701 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2761 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2821 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2881 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     2941 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     3001 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     3061 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccggtc
     3121 tcggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     3181 agtggcaccg agtcggtgct tttttttgca aaattttcca gatcgatttc ttcttcctct
     3241 gttcttcggc gttcaatttc tggggttttc tcttcgtttt ctgtaactga aacctaaaat
     3301 ttgacctaaa aaaaatctca aataatatga ttcagtggtt ttgtactttt cagttagttg
     3361 agttttgcag ttccgatgag ataaaccaat accatggtta tactagtctc aacttttgat
     3421 tcgctatttg cagtgcacct gtggcgttca tcacatcttt tgtgacactg tttgcactgg
     3481 tcattgctat tacaaaggac cttcctgatg ttgaaggaga tcgaaagtaa gtaactgcac
     3541 gcataaccat tttctttccg ctctttggct caatccattt gacagtcaaa gacaatgttt
     3601 aaccagctcc gtttgatata ttgtctttat gtgtttgttc aagcatgttt agttaatcat
     3661 gcctttgatt gatcttgaat aggttccaaa tatcaaccct ggcaacaaaa cttggagtga
     3721 gaaacattgc attcctcggt tctggacttc tgctagtaaa ttatgtttca gccatatcac
     3781 tagctttcta catgcctcag gtgaattcat ctatttccgt cttaactatt tcggttaatt
     3841 aaagcacgaa caccattact gcatgtagaa gcttgataaa ctatcgccac caatttattt
     3901 ttgttgcgat attgttactt tcctcagtat gcagctttga aaagaccaac cctcttatcc
     3961 tttaacaatg aacaggtttt tagaggtagc ttgatgattc ctgcacatgt gatcttggct
     4021 tcaggcttaa ttttccaggt aaagcattat gagatactct tatatctctt acatactttt
     4081 gagataatgc acaagaactt cataactata tgctttagtt tctgcatttg acactgccaa
     4141 attcattaat ctctaatatc tttgttgttg atctttggta gacatgggta ctagaaaaag
     4201 caaactacac caaggtaaaa tacttttgta caaacataaa ctcgttatca cggaacatca
     4261 atggagtgta tatctaacgg agtgtagaaa catttgatta ttgcaggaag ctatctcagg
     4321 atattatcgg tttatatgga atctcttcta cgcagagtat ctgttattcc ccttcctcta
     4381 gctttcaatt tcatggtgag gatatgcagt tttctttgta tatcattctt cttcttcttt
     4441 gtagcttgga gtcaaaatcg gttccttcat gtacatacat caaggatatg tccttctgaa
     4501 tttttatatc ttgcaataaa aatgcttgta ccaattgaaa caccagcttt ttgagttcta
     4561 tgatcactga cttggttcta accaaaaaaa aaaaaatgtt taatttacat atctaaaagt
     4621 aggtttaggg aaacctaaac agtaaaatat ttgtatatta ttcgaatttc actcatcata
     4681 aaaacttaaa ttgcaccata aaattttgtt ttactattaa tgatgtaatt tgtgtaactt
     4741 aagataaaaa taatattccg taagttaacc ggctaaaacc acgtataaac cagggaacct
     4801 gttaaaccgg ttctttactg gataaagaaa tgaaagccca tgtagacagc tccattagag
     4861 cccaaaccct aaatttctca tctatataaa aggagtgaca ttagggtttt tgttcgtcct
     4921 cttaaagctt ctcgttttct ctgccgtctc tctcattcgc gcgacgcaaa cgatcttcag
     4981 gtgatcttct ttctccaaat cctctctcat aactctgatt tcgtacttgt gtatttgagc
     5041 tcacgctctg tttctctcac cacagcctct agatggatta caaggaccac gacggggatt
     5101 acaaggacca cgacattgat tacaaggatg atgatgacaa gatggctccg aagaagaaga
     5161 ggaaggttgg catccacggg gtgccagctg ctgacaagaa gtactcgatc ggcctcgata
     5221 ttgggactaa ctctgttggc tgggccgtga tcaccgacga gtacaaggtg ccctcaaaga
     5281 agttcaaggt cctgggcaac accgatcggc attccatcaa gaagaatctc attggcgctc
     5341 tcctgttcga cagcggcgag acggctgagg ctacgcggct caagcgcacc gcccgcaggc
     5401 ggtacacgcg caggaagaat cgcatctgct acctgcagga gattttctcc aacgagatgg
     5461 cgaaggttga cgattctttc ttccacaggc tggaggagtc attcctcgtg gaggaggata
     5521 agaagcacga gcggcatcca atcttcggca acattgtcga cgaggttgcc taccacgaga
     5581 agtaccctac gatctaccat ctgcggaaga agctcgtgga ctccacagat aaggcggacc
     5641 tccgcctgat ctacctcgct ctggcccaca tgattaagtt caggggccat ttcctgatcg
     5701 agggggatct caacccggac aatagcgatg ttgacaagct gttcatccag ctcgtgcaga
     5761 cgtacaacca gctcttcgag gagaacccca ttaatgcgtc aggcgtcgac gcgaaggcta
     5821 tcctgtccgc taggctctcg aagtctcggc gcctcgagaa cctgatcgcc cagctgccgg
     5881 gcgagaagaa gaacggcctg ttcgggaatc tcattgcgct cagcctgggg ctcacgccca
     5941 acttcaagtc gaatttcgat ctcgctgagg acgccaagct gcagctctcc aaggacacat
     6001 acgacgatga cctggataac ctcctggccc agatcggcga tcagtacgcg gacctgttcc
     6061 tcgctgccaa gaatctgtcg gacgccatcc tcctgtctga tattctcagg gtgaacaccg
     6121 agattacgaa ggctccgctc tcagcctcca tgatcaagcg ctacgacgag caccatcagg
     6181 atctgaccct cctgaaggcg ctggtcaggc agcagctccc cgagaagtac aaggagatct
     6241 tcttcgatca gtcgaagaac ggctacgctg ggtacattga cggcggggcc tctcaggagg
     6301 agttctacaa gttcatcaag ccgattctgg agaagatgga cggcacggag gagctgctgg
     6361 tgaagctcaa tcgcgaggac ctcctgagga agcagcggac attcgataac ggcagcatcc
     6421 cacaccagat tcatctcggg gagctgcacg ctatcctgag gaggcaggag gacttctacc
     6481 ctttcctcaa ggataaccgc gagaagatcg agaagattct gactttcagg atcccgtact
     6541 acgtcggccc actcgctagg ggcaactccc gcttcgcttg gatgacccgc aagtcagagg
     6601 agacgatcac gccgtggaac ttcgaggagg tggtcgacaa gggcgctagc gctcagtcgt
     6661 tcatcgagag gatgacgaat ttcgacaaga acctgccaaa tgagaaggtg ctccctaagc
     6721 actcgctcct gtacgagtac ttcacagtct acaacgagct gactaaggtg aagtatgtga
     6781 ccgagggcat gaggaagccg gctttcctgt ctggggagca gaagaaggcc atcgtggacc
     6841 tcctgttcaa gaccaaccgg aaggtcacgg ttaagcagct caaggaggac tacttcaaga
     6901 agattgagtg cttcgattcg gtcgagatct ctggcgttga ggaccgcttc aacgcctccc
     6961 tggggaccta ccacgatctc ctgaagatca ttaaggataa ggacttcctg gacaacgagg
     7021 agaatgagga tatcctcgag gacattgtgc tgacactcac tctgttcgag gaccgggaga
     7081 tgatcgagga gcgcctgaag acttacgccc atctcttcga tgacaaggtc atgaagcagc
     7141 tcaagaggag gaggtacacc ggctggggga ggctgagcag gaagctcatc aacggcattc
     7201 gggacaagca gtccgggaag acgatcctcg acttcctgaa gagcgatggc ttcgcgaacc
     7261 gcaatttcat gcagctgatt cacgatgaca gcctcacatt caaggaggat atccagaagg
     7321 ctcaggtgag cggccagggg gactcgctgc acgagcatat cgcgaacctc gctggctcgc
     7381 cagctatcaa gaaggggatt ctgcagaccg tgaaggttgt ggacgagctg gtgaaggtca
     7441 tgggcaggca caagcctgag aacatcgtca ttgagatggc ccgggagaat cagaccacgc
     7501 agaagggcca gaagaactca cgcgagagga tgaagaggat cgaggagggc attaaggagc
     7561 tggggtccca gatcctcaag gagcacccgg tggagaacac gcagctgcag aatgagaagc
     7621 tctacctgta ctacctccag aatggccgcg atatgtatgt ggaccaggag ctggatatta
     7681 acaggctcag cgattacgac gtcgatcata tcgttccaca gtcattcctg aaggatgact
     7741 ccattgacaa caaggtcctc accaggtcgg acaagaaccg gggcaagtct gataatgttc
     7801 cttcagagga ggtcgttaag aagatgaaga actactggcg ccagctcctg aatgccaagc
     7861 tgatcacgca gcggaagttc gataacctca caaaggctga gaggggcggg ctctctgagc
     7921 tggacaaggc gggcttcatc aagaggcagc tggtcgagac acggcagatc actaagcacg
     7981 ttgcgcagat tctcgactca cggatgaaca ctaagtacga tgagaatgac aagctgatcc
     8041 gcgaggtgaa ggtcatcacc ctgaagtcaa agctcgtctc cgacttcagg aaggatttcc
     8101 agttctacaa ggttcgggag atcaacaatt accaccatgc ccatgacgcg tacctgaacg
     8161 cggtggtcgg cacagctctg atcaagaagt acccaaagct cgagagcgag ttcgtgtacg
     8221 gggactacaa ggtttacgat gtgaggaaga tgatcgccaa gtcggagcag gagattggca
     8281 aggctaccgc caagtacttc ttctactcta acattatgaa tttcttcaag acagagatca
     8341 ctctggccaa tggcgagatc cggaagcgcc ccctcatcga gacgaacggc gagacggggg
     8401 agatcgtgtg ggacaagggc agggatttcg cgaccgtcag gaaggttctc tccatgccac
     8461 aagtgaatat cgtcaagaag acagaggtcc agactggcgg gttctctaag gagtcaattc
     8521 tgcctaagcg gaacagcgac aagctcatcg cccgcaagaa ggactgggat ccgaagaagt
     8581 acggcgggtt cgacagcccc actgtggcct actcggtcct ggttgtggcg aaggttgaga
     8641 agggcaagtc caagaagctc aagagcgtga aggagctgct ggggatcacg attatggagc
     8701 gctccagctt cgagaagaac ccgatcgatt tcctggaggc gaagggctac aaggaggtga
     8761 agaaggacct gatcattaag ctccccaagt actcactctt cgagctggag aacggcagga
     8821 agcggatgct ggcttccgct ggcgagctgc agaaggggaa cgagctggct ctgccgtcca
     8881 agtatgtgaa cttcctctac ctggcctccc actacgagaa gctcaagggc agccccgagg
     8941 acaacgagca gaagcagctg ttcgtcgagc agcacaagca ttacctcgac gagatcattg
     9001 agcagatttc cgagttctcc aagcgcgtga tcctggccga cgcgaatctg gataaggtcc
     9061 tctccgcgta caacaagcac cgcgacaagc caatcaggga gcaggctgag aatatcattc
     9121 atctcttcac cctgacgaac ctcggcgccc ctgctgcttt caagtacttc gacacaacta
     9181 tcgatcgcaa gaggtacaca agcactaagg aggtcctgga cgcgaccctc atccaccagt
     9241 cgattaccgg cctctacgag acgcgcatcg acctgtctca gctcgggggc gacaagcggc
     9301 cagcggcgac gaagaaggcg gggcaggcga agaagaagaa gtgagctcag agctttcgtt
     9361 cgtatcatcg gtttcgacaa cgttcgtcaa gttcaatgca tcagtttcat tgcgcacaca
     9421 ccagaatcct actgagtttg agtattatgg cattgggaaa actgtttttc ttgtaccatt
     9481 tgttgtgctt gtaatttact gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg
     9541 aaatggatgg agaagagtta atgaatgata tggtcctttt gttcattctc aaattaatat
     9601 tatttgtttt ttctcttatt tgttgtgtgt tgaatttgaa attataagag atatgcaaac
     9661 attttgtttt gagtaaaaat gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag
     9721 gagtaaaaca cttgtagttg taccattatg cttattcact aggcaacaaa tatattttca
     9781 gacctagaaa agctgcaaat gttactgaat acaagtatgt cctcttgtgt tttagacatt
     9841 tatgaacttt cctttatgta attttccaga atccttgtca gattctaatc attgctttat
     9901 aattatagtt atactcatgg atttgtagtt gagtatgaaa atatttttta atgcatttta
     9961 tgacttgcca attgggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    10021 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    10081 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    10141 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    10201 ggctagagca gcttgccaac atggtggagc acgacactct cgtctactcc aagaatatca
    10261 aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg
    10321 gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa
    10381 aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
    10441 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
    10501 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgaacatggt ggagcacgac
    10561 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
    10621 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
    10681 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
    10741 aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca
    10801 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
    10861 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
    10921 ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctgaaa tcaccagtct
    10981 ctctctacaa atctatctct ctcgagtcta ccatgagccc agaacgacgc ccggccgaca
    11041 tccgccgtgc caccgaggcg gacatgccgg cggtctgcac catcgtcaac cactacatcg
    11101 agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc gcaggagtgg acggacgacc
    11161 tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga ggtggacggc gaggtcgccg
    11221 gcatcgccta cgcgggcccc tggaaggcac gcaacgccta cgactggacg gccgagtcga
    11281 ccgtgtacgt ctccccccgc caccagcgga cgggactggg ctccacgctc tacacccacc
    11341 tgctgaagtc cctggaggca cagggcttca agagcgtggt cgctgtcatc gggctgccca
    11401 acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc cccccgcggc atgctgcggg
    11461 cggccggctt caagcacggg aactggcatg acgtgggttt ctggcagctg gacttcagcc
    11521 tgccggtacc gccccgtccg gtcctgcccg tcaccgagat ttgactcgag tttctccata
    11581 ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt
    11641 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
    11701 aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcccccg aattaattcg
    11761 gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
    11821 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
    11881 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
    11941 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
    12001 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
    12061 gacagagcgt tgctgcctgt gatcaccgcg gtttcaaaat cggctccgtc gatactatgt
    12121 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
    12181 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
    12241 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
    12301 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
    12361 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
    12421 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
    12481 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
    12541 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
    12601 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
    12661 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
    12721 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
    12781 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
    12841 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
    12901 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
    12961 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
    13021 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagaatgcat
    13081 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg ctgagagatc ccctcataat
    13141 ttccccaaag cgtaaccatg tgtgaataaa ttttgagcta gtagggttgc agccacgagt
    13201 aagtcttccc ttgttattgt gtagccagaa tgccgcaaaa cttccatgcc taagcgaact
    13261 gttgagagta cgtttcgatt tctgactgtg ttagcctgga agtgcttgtc ccaaccttgt
    13321 ttctgagcat gaacgcccgc aagccaacat gttagttgaa gcatcagggc gattagcagc
    13381 atgatatcaa aacgctctga gctgctcgtt cggctatggc gtaggcctag tccgtaggca
    13441 ggacttttca agtctcggaa ggtttcttca atctgcattc gcttcgaata gatattaaca
    13501 agttgtttgg gtgttcgaat ttcaacaggt aagttagttg ctagaaccca tggctccttt
    13561 gccgacgctg agtagatttt aggtgacggg tggtgacaat gagtccgtgt cgagcgctga
    13621 ttttttcggc ctttagagcg agatttatac aatagaattt ggcatgagat tggattgctt
    13681 ttagtcagcc tcttatagcc taaagtcttt gagtgactag atgacatatc atgtaagttg
    13741 ctgataggtt tccagttttc cgctcctagg tctgcatatt gtacttttcc tcttactcga
    13801 cttaaccagt accaacccag cttctcaacg gatttatacc atggcacttt aaagccagca
    13861 tcactgacaa tgagcggtgt ggtgttactc ggtagaatgc tcgcaaggtc ggctagaaat
    13921 tggtcatgag ctttctttga acattgctct gaaagcggga acgctttctc ataaagagta
    13981 acagaacgac cgtgtagtgc gactgaagct cgcaatacca taagtcgttt ttgctcacga
    14041 atatcagacc agtcaacaag tacaatgggc atcgtattgc ccgaacagat aaagctagca
    14101 tgccaacggt atacagcgag tcgctctttg tggaggtgac gattacctaa caatcggtcg
    14161 attcgtttga tgttatgttt tgttctcgct ttggttggca ggttacggcc aagttcggta
    14221 agagtgagag ttttacagtc aagtaatgcg tggcaagcca acgttaagct gttgagtcgt
    14281 tttaagtgta attcggggca gaattggtaa agagagtcgt gtaaaatatc gagttcgcac
    14341 atcttgttgt ctgattattg atttttcgcg aaaccatttg atcatatgac aagatgtgta
    14401 tccaccttaa cttaatgatt tttaccaaaa tcattagggg attcatcagc actgagcgtc
    14461 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    14521 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    14581 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
    14641 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    14701 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    14761 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    14821 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    14881 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    14941 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    15001 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    15061 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    15121 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    15181 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    15241 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
    15301 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
    15361 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
    15421 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
    15481 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
    15541 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
    15601 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
    15661 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
    15721 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
    15781 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
    15841 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
    15901 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaca gaccttttcg
    15961 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
    16021 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
    16081 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
    16141 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
    16201 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
    16261 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
    16321 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
    16381 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
    16441 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
    16501 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
    16561 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
    16621 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
    16681 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
    16741 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
    16801 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
    16861 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
    16921 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
    16981 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
    17041 cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg
    17101 ggggttccag tgccattgca gggccggcag gcaacccagc cgcttacgcc tggccaaccg
    17161 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
    17221 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
    17281 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
    17341 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
    17401 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
    17461 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
    17521 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
    17581 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
    17641 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
    17701 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
    17761 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
    17821 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
    17881 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
    17941 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
    18001 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
    18061 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
    18121 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
    18181 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
    18241 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
    18301 acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca
    18361 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
    18421 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
    18481 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
    18541 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
    18601 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
    18661 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
    18721 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
    18781 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
    18841 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
    18901 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
    18961 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
    19021 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
    19081 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
    19141 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
    19201 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
    19261 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
    19321 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
    19381 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
//
