LOCUS       sequence-446482-       20026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single 
            intron-containing Cas9, EC1 promoter, hygromycin and red fluorescent
            seed selection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20026)
  TITLE     Takeuchi CRISPR/Cas9 Plasmids for multi-gene knockout in Arabidopsis
REFERENCE   2  (bases 1 to 20026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHEE-R-hyg9si
FEATURES             Location/Qualifiers
     source          1..20026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             531..1238
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     944..963
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2181..2972
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        3124..3199
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             5121..5186
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5193..5213
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5238..9338
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5238..9338
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             9251..9259
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9339..9386
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(10043..10059)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10043..10059)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10056..10078)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10067..10083
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10091..10097,10098..10115,10116..10121))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10136..10157
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10348..11024
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10961..10981
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11091..12116
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    12157..12331
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12409..12433
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12858..13652
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      15077..15665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15566..15585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15819..15836
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15851..15991
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15917..15936)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     16077..16099
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      16335..16529
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16595..17668)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(18097..18726)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagtttc ttgaaaccaa attaacatag
       61 ggtttttatt taaataaaag tttaaccttc tttttaaaaa attgttcata gtgtcatgtc
      121 agaacaagag ctacaaatca cacatagcat gcataagcgg agctatgatg agtggtattg
      181 ttttgttcgt cacttgtcac tcttttccaa cacataatcc cgacaacaac gtaagagcat
      241 ctctctctct ccacacacac tcatgcatgc atgcattctt acacgtgatt gccatgcaaa
      301 tctcctttct cacctataaa tacaaaccaa cccttcacta cactcttcac tcaaaccaaa
      361 acaagaaaac atacacaaat agcaaaacaa gggcgaattc tcaacacaac atatacaaaa
      421 caaacgaatc tcaagcaatc aagcattcta cttctattgc agcaatttaa atcatttctt
      481 ttaaagcaaa agcaattttc tgaaaatttt caccatttac gaacgatagc atggtgagca
      541 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      601 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      661 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      721 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      781 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      841 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      901 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
      961 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1021 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1081 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1141 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1201 gccgccactc caccggcggc atggacgagc tgtacaagaa ggacgagctg taagcgccgc
     1261 ccggctgcat cgctgaaatc accagtctct ctctacaaat ctatctctct ctataataat
     1321 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     1381 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     1441 ctaattccta aaaccaaaat ccagtggtac caagcttcga cttgccttcc gcacaataca
     1501 tcatttcttc ttagcttttt ttcttcttct tcgttcatac agtttttttt tgtttatcag
     1561 cttacatttt cttgaaccgt agctttcgtt ttcttctttt taactttcca ttcggagttt
     1621 ttgtatcttg tttcatagtt tgtcccagga ttagaatgat taggcatcga accttcaaga
     1681 atttgattga ataaaacatc ttcattctta agatatgaag ataatcttca aaaggcccct
     1741 gggaatctga aagaagagaa gcaggcccat ttatatggga aagaacaata gtatttctta
     1801 tataggccca tttaagttga aaacaatctt caaaagtccc acatcgctta gataagaaaa
     1861 cgaagctgag tttatataca gctagagtcg aagtagtgat tgggagacca acccagtgga
     1921 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     1981 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2041 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2101 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2161 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     2221 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2281 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2341 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2401 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2461 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2521 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2581 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2641 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2701 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2761 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2821 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2881 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     2941 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     3001 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     3061 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccggtc
     3121 tcggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     3181 agtggcaccg agtcggtgct tttttttgca aaattttcca gatcgatttc ttcttcctct
     3241 gttcttcggc gttcaatttc tggggttttc tcttcgtttt ctgtaactga aacctaaaat
     3301 ttgacctaaa aaaaatctca aataatatga ttcagtggtt ttgtactttt cagttagttg
     3361 agttttgcag ttccgatgag ataaaccaat accatggtta tactagtgaa taaaagcatt
     3421 tgcgtttggt ttatcattgc gtttatacaa ggacagagat ccactgagct ggaatagctt
     3481 aaaaccatta tcagaacaaa ataaaccatt ttttgttaag aatcagagca tagtaaacaa
     3541 cagaaacaac ctaagagagg taacttgtcc aagaagatag ctaattatat ctattttata
     3601 aaagttatca tagtttgtaa gtcacaaaag atgcaaataa cagagaaact aggagacttg
     3661 agaatataca ttcttgtata tttgtattcg agattgtgaa aatttgacca taagtttaaa
     3721 ttcttaaaaa gatatatctg atctaggtga tggttataga ctgtaatttt accacatgtt
     3781 taatgatgga tagtgacaca catgacacat cgacaacact atagcatctt atttagatta
     3841 caacatgaaa tttttctgta atacatgtct ttgtacataa tttaaaagta attcctaaga
     3901 aatatattta tacaaggagt ttaaagaaaa catagcataa agttcaatga gtagtaaaaa
     3961 ccatatacag tatatagcat aaagttcaat gagtttatta caaaagcatt ggttcacttt
     4021 ctgtaacacg acgttaaacc ttcgtctcca ataggagcgc tactgattca acatgccaat
     4081 atatactaaa tacgtttcta cagtcaaatg ctttaacgtt tcatgattaa gtgactattt
     4141 accgtcaatc ctttcccatt cctcccacta atccaacttt ttaattactc ttaaatcacc
     4201 actaagctag taacgcctat catgaattag ctctactaaa tctagcaacc tttcaaattt
     4261 gcagtattgc aggtgtctct gtgtctttaa aatagttgcc ttatgatttc ttcggtttca
     4321 agatgatcaa atagttatag atttcatgct cacacatgct cattagatgt gtacatactt
     4381 tacttaccca aatctatttt ctcgcaaaga ttttgatggt aaagctgatt tggttctatt
     4441 gaactaaatc aaacgagttt cagactgagt gattctaatc cggcccatta gcccctaaac
     4501 agacccacta attacgcagc ttttaataga gtaattacac ctagtttacc cactaaacca
     4561 ctaagcacta attatctcac aatctaatga gcttccctcg taattacttg ggctttcact
     4621 ctaccattta tttgtaacag tcaagtctct actgtctcta tataaactct ctaaagttaa
     4681 cacacaattc tcatcacaaa caaatcaacc aaagcaactt ctactctttc ttctttcgac
     4741 cttatcaatc tgttgagaaa tctagatgca gccttctctt aaacgtatga aaattgagcc
     4801 atcttctcaa aaggtaaatt tctgtgttcc ttattctctc aaaatcttcg attttgtttt
     4861 cgttcgatcc caatttcgta tatgttcttt ggtttagatt ctgttaatct tagatcgaag
     4921 acgattttct gggtttgatc gttagatatc atcttaattc tcgattaggg tttcatagat
     4981 atcatccgat ttgttcaaat aatttgagtt ttgtcgaata attactcttc gatttgtgat
     5041 ttctatctag atctggtgtt agtttctagt ttgtgcgatc gaatttgtcg attaatctga
     5101 gtttttctga ttaacagatg gattacaagg accacgacgg ggattacaag gaccacgaca
     5161 ttgattacaa ggatgatgat gacaagatgg ctccgaagaa gaagaggaag gttggcatcc
     5221 acggggtgcc agctgctgac aagaagtact cgatcggcct cgatattggg actaactctg
     5281 ttggctgggc cgtgatcacc gacgagtaca aggtgccctc aaagaagttc aaggtcctgg
     5341 gcaacaccga tcggcattcc atcaagaaga atctcattgg cgctctcctg ttcgacagcg
     5401 gcgagacggc tgaggctacg cggctcaagc gcaccgcccg caggcggtac acgcgcagga
     5461 agaatcgcat ctgctacctg caggagattt tctccaacga gatggcgaag gttgacgatt
     5521 ctttcttcca caggctggag gagtcattcc tcgtggagga ggataagaag cacgagcggc
     5581 atccaatctt cggcaacatt gtcgacgagg ttgcctacca cgagaagtac cctacgatct
     5641 accatctgcg gaagaagctc gtggactcca cagataaggc ggacctccgc ctgatctacc
     5701 tcgctctggc ccacatgatt aagttcaggg gccatttcct gatcgagggg gatctcaacc
     5761 cggacaatag cgatgttgac aagctgttca tccagctcgt gcagacgtac aaccagctct
     5821 tcgaggagaa ccccattaat gcgtcaggcg tcgacgcgaa ggctatcctg tccgctaggc
     5881 tctcgaagtc tcggcgcctc gagaacctga tcgcccagct gccgggcgag aagaagaacg
     5941 gcctgttcgg gaatctcatt gcgctcagcc tggggctcac gcccaacttc aagtcgaatt
     6001 tcgatctcgc tgaggacgcc aagctgcagc tctccaagga cacatacgac gatgacctgg
     6061 ataacctcct ggcccagatc ggcgatcagt acgcggacct gttcctcgct gccaagaatc
     6121 tgtcggacgc catcctcctg tctgatattc tcagggtgaa caccgagatt acgaaggctc
     6181 cgctctcagc ctccatgatc aagcgctacg acgagcacca tcaggatctg accctcctga
     6241 aggcgctggt caggcagcag ctccccgaga agtacaagga gatcttcttc gatcagtcga
     6301 agaacggcta cgctgggtac attgacggcg gggcctctca ggaggagttc tacaagttca
     6361 tcaagccgat tctggagaag atggacggca cggaggagct gctggtgaag ctcaatcgcg
     6421 aggacctcct gaggaagcag cggacattcg ataacggcag catcccacac cagattcatc
     6481 tcggggagct gcacgctatc ctgaggaggc aggaggactt ctaccctttc ctcaaggata
     6541 accgcgagaa gatcgagaag attctgactt tcaggatccc gtactacgtc ggcccactcg
     6601 ctaggggcaa ctcccgcttc gcttggatga cccgcaagtc agaggagacg atcacgccgt
     6661 ggaacttcga ggaggtggtc gacaagggcg ctagcgctca gtcgttcatc gagaggatga
     6721 cgaatttcga caagaacctg ccaaatgaga aggtgctccc taagcactcg ctcctgtacg
     6781 agtacttcac agtctacaac gagctgacta aggtgaagta tgtgaccgag ggcatgagga
     6841 agccggcttt cctgtctggg gagcagaaga aggccatcgt ggacctcctg ttcaagacca
     6901 accggaaggt cacggttaag cagctcaagg aggactactt caagaagatt gagtgcttcg
     6961 attcggtcga gatctctggc gttgaggacc gcttcaacgc ctccctgggg acctaccacg
     7021 atctcctgaa gatcattaag gataaggact tcctggacaa cgaggagaat gaggatatcc
     7081 tcgaggacat tgtgctgaca ctcactctgt tcgaggaccg ggagatgatc gaggagcgcc
     7141 tgaagactta cgcccatctc ttcgatgaca aggtcatgaa gcagctcaag aggaggaggt
     7201 acaccggctg ggggaggctg agcaggaagc tcatcaacgg cattcgggac aagcagtccg
     7261 ggaagacgat cctcgacttc ctgaagagcg atggcttcgc gaaccgcaat ttcatgcagc
     7321 tgattcacga tgacagcctc acattcaagg aggatatcca gaaggctcag gtgagcggcc
     7381 agggggactc gctgcacgag catatcgcga acctcgctgg ctcgccagct atcaagaagg
     7441 ggattctgca gaccgtgaag gttgtggacg agctggtgaa ggtcatgggc aggcacaagc
     7501 ctgagaacat cgtcattgag atggcccggg agaatcagac cacgcagaag ggccagaaga
     7561 actcacgcga gaggatgaag aggatcgagg agggcattaa ggagctgggg tcccagatcc
     7621 tcaaggagca cccggtggag aacacgcagc tgcagaatga gaagctctac ctgtactacc
     7681 tccagaatgg ccgcgatatg tatgtggacc aggagctgga tattaacagg ctcagcgatt
     7741 acgacgtcga tcatatcgtt ccacagtcat tcctgaagga tgactccatt gacaacaagg
     7801 tcctcaccag gtcggacaag aaccggggca agtctgataa tgttccttca gaggaggtcg
     7861 ttaagaagat gaagaactac tggcgccagc tcctgaatgc caagctgatc acgcagcgga
     7921 agttcgataa cctcacaaag gctgagaggg gcgggctctc tgagctggac aaggcgggct
     7981 tcatcaagag gcagctggtc gagacacggc agatcactaa gcacgttgcg cagattctcg
     8041 actcacggat gaacactaag tacgatgaga atgacaagct gatccgcgag gtgaaggtca
     8101 tcaccctgaa gtcaaagctc gtctccgact tcaggaagga tttccagttc tacaaggttc
     8161 gggagatcaa caattaccac catgcccatg acgcgtacct gaacgcggtg gtcggcacag
     8221 ctctgatcaa gaagtaccca aagctcgaga gcgagttcgt gtacggggac tacaaggttt
     8281 acgatgtgag gaagatgatc gccaagtcgg agcaggagat tggcaaggct accgccaagt
     8341 acttcttcta ctctaacatt atgaatttct tcaagacaga gatcactctg gccaatggcg
     8401 agatccggaa gcgccccctc atcgagacga acggcgagac gggggagatc gtgtgggaca
     8461 agggcaggga tttcgcgacc gtcaggaagg ttctctccat gccacaagtg aatatcgtca
     8521 agaagacaga ggtccagact ggcgggttct ctaaggagtc aattctgcct aagcggaaca
     8581 gcgacaagct catcgcccgc aagaaggact gggatccgaa gaagtacggc gggttcgaca
     8641 gccccactgt ggcctactcg gtcctggttg tggcgaaggt tgagaagggc aagtccaaga
     8701 agctcaagag cgtgaaggag ctgctgggga tcacgattat ggagcgctcc agcttcgaga
     8761 agaacccgat cgatttcctg gaggcgaagg gctacaagga ggtgaagaag gacctgatca
     8821 ttaagctccc caagtactca ctcttcgagc tggagaacgg caggaagcgg atgctggctt
     8881 ccgctggcga gctgcagaag gggaacgagc tggctctgcc gtccaagtat gtgaacttcc
     8941 tctacctggc ctcccactac gagaagctca agggcagccc cgaggacaac gagcagaagc
     9001 agctgttcgt cgagcagcac aagcattacc tcgacgagat cattgagcag atttccgagt
     9061 tctccaagcg cgtgatcctg gccgacgcga atctggataa ggtcctctcc gcgtacaaca
     9121 agcaccgcga caagccaatc agggagcagg ctgagaatat cattcatctc ttcaccctga
     9181 cgaacctcgg cgcccctgct gctttcaagt acttcgacac aactatcgat cgcaagaggt
     9241 acacaagcac taaggaggtc ctggacgcga ccctcatcca ccagtcgatt accggcctct
     9301 acgagacgcg catcgacctg tctcagctcg ggggcgacaa gcggccagcg gcgacgaaga
     9361 aggcggggca ggcgaagaag aagaagtgag ctcagagctt tcgttcgtat catcggtttc
     9421 gacaacgttc gtcaagttca atgcatcagt ttcattgcgc acacaccaga atcctactga
     9481 gtttgagtat tatggcattg ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat
     9541 ttactgtgtt ttttattcgg ttttcgctat cgaactgtga aatggaaatg gatggagaag
     9601 agttaatgaa tgatatggtc cttttgttca ttctcaaatt aatattattt gttttttctc
     9661 ttatttgttg tgtgttgaat ttgaaattat aagagatatg caaacatttt gttttgagta
     9721 aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat atgaggagta aaacacttgt
     9781 agttgtacca ttatgcttat tcactaggca acaaatatat tttcagacct agaaaagctg
     9841 caaatgttac tgaatacaag tatgtcctct tgtgttttag acatttatga actttccttt
     9901 atgtaatttt ccagaatcct tgtcagattc taatcattgc tttataatta tagttatact
     9961 catggatttg tagttgagta tgaaaatatt ttttaatgca ttttatgact tgccaattga
    10021 ttgacaacga attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca
    10081 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    10141 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    10201 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattggct
    10261 agagcagctt gccaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga
    10321 tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa
    10381 cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga
    10441 aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc
    10501 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
    10561 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgaa catggtggag cacgacactc
    10621 tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt
    10681 ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
    10741 tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag
    10801 gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
    10861 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
    10921 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
    10981 cctctatata aggaagttca tttcatttgg agaggacacg ctgaaatcac cagtctctct
    11041 ctacaaatct atctctctcg agctttcgca gatccggggg gcaatgagat atgaaaaagc
    11101 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
    11161 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
    11221 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
    11281 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggagttta
    11341 gcgagagcct gacctattgc atctcccgcc gttcacaggg tgtcacgttg caagacctgc
    11401 ctgaaaccga actgcccgct gttctacaac cggtcgcgga ggctatggat gcgatcgctg
    11461 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
    11521 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
    11581 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
    11641 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
    11701 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
    11761 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
    11821 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgcca cgactccggg
    11881 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
    11941 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
    12001 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
    12061 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag aaatagagta
    12121 gatgccgacc gggatctgtc gatcgacaag ctcgagtttc tccataataa tgtgtgagta
    12181 gttcccagat aagggaatta gggttcctat agggtttcgc tcatgtgttg agcatataag
    12241 aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct
    12301 aaaaccaaaa tccagtacta aaatccagat cccccgaatt aattcggcgt taattcagta
    12361 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
    12421 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
    12481 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
    12541 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
    12601 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
    12661 gcctgtgatc accgcggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt
    12721 gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt
    12781 gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa
    12841 gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg
    12901 aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg
    12961 tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct
    13021 atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa
    13081 aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg
    13141 gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt
    13201 atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata
    13261 gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg
    13321 attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt
    13381 taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca
    13441 acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg
    13501 cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag
    13561 aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa
    13621 aatattatat tttactggat gaattgtttt agtacctaga atgcatgacc aaaatccctt
    13681 aacgtgagtt ttcgttccac tgagcgctga gagatcccct cataatttcc ccaaagcgta
    13741 accatgtgtg aataaatttt gagctagtag ggttgcagcc acgagtaagt cttcccttgt
    13801 tattgtgtag ccagaatgcc gcaaaacttc catgcctaag cgaactgttg agagtacgtt
    13861 tcgatttctg actgtgttag cctggaagtg cttgtcccaa ccttgtttct gagcatgaac
    13921 gcccgcaagc caacatgtta gttgaagcat cagggcgatt agcagcatga tatcaaaacg
    13981 ctctgagctg ctcgttcggc tatggcgtag gcctagtccg taggcaggac ttttcaagtc
    14041 tcggaaggtt tcttcaatct gcattcgctt cgaatagata ttaacaagtt gtttgggtgt
    14101 tcgaatttca acaggtaagt tagttgctag aacccatggc tcctttgccg acgctgagta
    14161 gattttaggt gacgggtggt gacaatgagt ccgtgtcgag cgctgatttt ttcggccttt
    14221 agagcgagat ttatacaata gaatttggca tgagattgga ttgcttttag tcagcctctt
    14281 atagcctaaa gtctttgagt gactagatga catatcatgt aagttgctga taggtttcca
    14341 gttttccgct cctaggtctg catattgtac ttttcctctt actcgactta accagtacca
    14401 acccagcttc tcaacggatt tataccatgg cactttaaag ccagcatcac tgacaatgag
    14461 cggtgtggtg ttactcggta gaatgctcgc aaggtcggct agaaattggt catgagcttt
    14521 ctttgaacat tgctctgaaa gcgggaacgc tttctcataa agagtaacag aacgaccgtg
    14581 tagtgcgact gaagctcgca ataccataag tcgtttttgc tcacgaatat cagaccagtc
    14641 aacaagtaca atgggcatcg tattgcccga acagataaag ctagcatgcc aacggtatac
    14701 agcgagtcgc tctttgtgga ggtgacgatt acctaacaat cggtcgattc gtttgatgtt
    14761 atgttttgtt ctcgctttgg ttggcaggtt acggccaagt tcggtaagag tgagagtttt
    14821 acagtcaagt aatgcgtggc aagccaacgt taagctgttg agtcgtttta agtgtaattc
    14881 ggggcagaat tggtaaagag agtcgtgtaa aatatcgagt tcgcacatct tgttgtctga
    14941 ttattgattt ttcgcgaaac catttgatca tatgacaaga tgtgtatcca ccttaactta
    15001 atgattttta ccaaaatcat taggggattc atcagcactg agcgtcagac cccgtagaaa
    15061 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    15121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    15181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
    15241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    15301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    15361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    15421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    15481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    15541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    15601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    15661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    15721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    15781 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    15841 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
    15901 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
    15961 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
    16021 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
    16081 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
    16141 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
    16201 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
    16261 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc
    16321 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
    16381 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
    16441 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
    16501 ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg
    16561 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
    16621 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
    16681 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
    16741 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
    16801 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
    16861 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
    16921 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
    16981 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
    17041 ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
    17101 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
    17161 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
    17221 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
    17281 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
    17341 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
    17401 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
    17461 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
    17521 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
    17581 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
    17641 ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc
    17701 attgcagggc cggcaggcaa cccagccgct tacgcctggc caaccgcccg ttcctccaca
    17761 catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt
    17821 agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga
    17881 tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct
    17941 tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca
    18001 ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt
    18061 ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc
    18121 agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt
    18181 tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat
    18241 gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat
    18301 cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt
    18361 aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat
    18421 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
    18481 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
    18541 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
    18601 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
    18661 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
    18721 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
    18781 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
    18841 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
    18901 atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg
    18961 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
    19021 ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg
    19081 tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc
    19141 tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt
    19201 tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga
    19261 gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg
    19321 tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca
    19381 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
    19441 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
    19501 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
    19561 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
    19621 cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc
    19681 ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag
    19741 tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg
    19801 cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg
    19861 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc
    19921 gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg
    19981 cccttttaaa tatccgatta ttctaataaa cgctcttttc tcttag
//
