LOCUS       sequence-446483-        8643 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus transfer plasmid for expression of full length human 
            PHD2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8643)
  AUTHORS   Gurol KC, Jursa T, Cho EJ, Fast W, Dalby KN, Smith DR, Mukhopadhyay 
            S
  TITLE     PHD2 enzyme is an intracellular manganese sensor that initiates the 
            homeostatic response against elevated manganese.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jun 25;121(26):e2402538121. doi: 
            10.1073/pnas.2402538121. Epub 2024 Jun 21.
  PUBMED    38905240
REFERENCE   2  (bases 1 to 8643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-06-25"; volume: "121(26):e2402538121. 
            doi"; pages: " 10.1073/pnas.2402538121. Epub 2024 Jun 21"
COMMENT     Sequence Label: pLJM1-PHD2
FEATURES             Location/Qualifiers
     source          1..8643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    314..439
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             486..666
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(667..893)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(921..941)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(921..939)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(960..976)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(960..976)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(973..995)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    984..1000
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1008..1014,1015..1032,1033..1038))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1053..1074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1191..1208)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1362..1950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1442..1461)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2121..2912,2913..2981))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2744..2763
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2982..3086)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3112..3567)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3249..3270)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3461..3480
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3694..3716
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3708..3725
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3709..3725
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3735..3754
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3735..3753
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3774..3909
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3949..4070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3960..3979)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4014..4033
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4142..4375
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(4503..5102)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPSA"
     primer_bind     complement(4586..4606)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5083..5102
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5124..5634)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5187..5205)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    5683..5800
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7126..7152)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(7192..7395)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7221..7241)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(7684..7704)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(8002..8046)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8231..8464
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt
       61 gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg
      121 tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca
      181 ggccaggatt aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt
      241 tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc
      301 cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa
      361 tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc
      421 gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc
      481 gccactgcta gagattttcc acactgacta aaagggtctg agggatctct agttaccaga
      541 gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt
      601 aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag
      661 agacccgttt atgtatcgag ctaggcactt aaatacaata tctctgcaat gcggcaattc
      721 agtggttcgt ccaatccatg tcagacccgt ctgttgcctt cctaataagg cacgatcgta
      781 ccaccttact tccaccaatc ggcatgcacg gtgctttttc tctccttgta aggcatgttg
      841 ctaactcatc gttaccatgt tgcaagacta caagagtatt gcataagact acattaagct
      901 tgcagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
      961 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     1021 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     1081 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     1141 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     1201 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1261 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1321 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1381 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1441 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1501 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1561 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1621 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1681 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1741 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1801 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1861 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1921 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1981 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2041 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2101 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2161 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     2221 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     2281 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     2341 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     2401 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     2461 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     2521 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     2581 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     2641 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     2701 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     2761 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     2821 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     2881 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     2941 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3001 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3061 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     3121 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     3181 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     3241 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     3301 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     3361 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     3421 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     3481 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     3541 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     3601 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     3661 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     3721 ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtacgtcc ctaggcctcc
     3781 aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc
     3841 ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag
     3901 gggcgggatg ggcggagtta ggggcgggat agctagagcc agacatgata agatacattg
     3961 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     4021 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt cctctcactc
     4081 tctgatattc atttctttgc aagttataaa tactgaataa taagatgaca tgaactacta
     4141 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
     4201 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
     4261 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
     4321 ccagtacaag caaaaagcag atcttgtctt cgttgggagt gaattagccc ttccagtccc
     4381 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
     4441 ggtaccgatg catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg
     4501 cgtcaggcac tgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg
     4561 tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc
     4621 tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg
     4681 ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg
     4741 ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg
     4801 gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg
     4861 ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg
     4921 atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg
     4981 cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg
     5041 cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc
     5101 atggtaagct ccggtggatc cccctgggga gagaggtcgg tgattcggtc aacgagggag
     5161 ccgactgccg acgtgcgctc cggaggcttg cagaatgcgg aacaccgcgc gggcaggaac
     5221 agggcccaca ctaccgcccc acaccccgcc tcccgcaccg ccccttcccg gccgctgctc
     5281 tcggcgcgcc ctgctgagca gccgctattg gccacagccc atcgcggtcg gcgcgctgcc
     5341 attgctccct ggcgctgtcc gtctgcgagg gtactagtga gacgtgcggc ttccgtttgt
     5401 cacgtccggc acgccgcgaa ccgcaaggaa ccttcccgac ttaggggcgg agcaggaagc
     5461 gtcgccgggg ggcccacaag ggtagcggcg aagatccggg tgacgctgcg aacggacgtg
     5521 aagaatgtgc gagacccagg gtcggcgccg ctgcgtttcc cggaaccacg cccagagcag
     5581 ccgcgtccct gcgcaaaccc agggctgcct tggaaaaggc gcaaccccaa ccccctcgag
     5641 ccgcggccaa agtggatctc tgctgtccct gtaataaacc cgaaaatttt gaatttttgt
     5701 aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc
     5761 ccctgcactg taccccccaa tccccccttt tcttttaaaa ttgtggatga atactgccat
     5821 ttgtctcgag gtcgagaatt cctagaagac gtctttaccg accgaatctg aaggtttatt
     5881 gagttcaacc ctcacacctt tttcacctgt tagatatttt actttagctc gtgctctctc
     5941 atctgcatca aaataccaaa cagttattgc gtaccttgta gcatatgctg gttgtacttc
     6001 atgagggttg cgacggtcag accagaaaaa cagcagtcta tcaaatttgg gttcaatgtc
     6061 agcaaactgg gctttgcctt ctggaaaaat tcgaagtata cctccactta ccttggcatc
     6121 ccagtcttta ttaagataat atatacatgt cacacatctt ccatctccat ttggattatc
     6181 aacatgacgt acataacccg ttccattgcc cggataacaa gcaaccatgg ctttcgtccg
     6241 gccattgatt ttgtagctgc ccagcttccc gttacagtgg cgtatcaggt cgtccatgct
     6301 gctcatgagc agcccaatgg tttcgcagcc gggctccttg ccctcgatcc aggtgatctt
     6361 atcgcctcgg atgtccttgg acgagtcact cttctggctg accagctgcc cgtccgtgaa
     6421 cttcccggtg tcgtgcaggg cgcgcacctc gtcgccgatc tgctgtccgg tctccttgcc
     6481 gaggaagtcg tccaccacac agatgccgtg cttgttcatg cacggcacga tgtactcgag
     6541 cgccagcttc agcgccggca ggggcttcgt ctgcccgttg ggccgcaggc cgccgccggg
     6601 gctcagcgca tccccgggcg tgttgcttgg ggggtacagg ttcgccttct cctggaacag
     6661 cgatgagcgg gccggcggct cctccttgcc gggctcggct tcggcagcca ccgccgagcc
     6721 ctggccgccg gcggccgcac gacacggcga cgcggccgcc gctgggtcgg ccgggggctt
     6781 ggcctttact tttcccttgg ccgcgtcccc ggaggcgttg tcccggcgcg ccgctgcctt
     6841 cctgggctcc cgggccccgg ccctgggcgg cggcactgca gccggcggcg cggggccgga
     6901 atgctggtgt gggcccactc cgtggccgag ggcgccctcg ctgccctggc acacgagctt
     6961 gtgcttcttc cagtcctgac gctggtgctc cttgcagcag tagaaggagc tgcggcagcg
     7021 gctgcagcgc agcaggttct ccatcttccc gcacagctcg cagtactgcc ggtctcgctc
     7081 gctcgggctc ggcccgccgg gcccgccgct gtcattggcg gatccagcat aatctggaac
     7141 atcatatgga tacatggtgg caagctagcg gatctgacgg ttcactaaac cagctctgct
     7201 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
     7261 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
     7321 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
     7381 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     7441 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     7501 gtcaataggg ggcgtacttg gcatatgata gtccatttta aaacataatt ttaaaactgc
     7561 aaactaccca agaaattatt actttctacg tcacgtattt tgtactaata tctttgtgtt
     7621 tacagtcaaa ttaattccaa ttatctctct aacagccttg tatcgtatat gcaaatatga
     7681 aggaatcatg ggaaataggc cctcggtgaa gggggcggcc gctcgaggct agtctcgtga
     7741 tcgataccgt cgagatccgt tcactaatcg aatggatctg tctctgtctc tctctccacc
     7801 ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa
     7861 cgataatggt gaatatccct gcctaactct attcactata gaaagtacag caaaaactat
     7921 tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt
     7981 tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc
     8041 attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt
     8101 gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt
     8161 attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc
     8221 ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg
     8281 gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct
     8341 cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag
     8401 cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc
     8461 tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc
     8521 tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc
     8581 cctcatatct cctcctccag gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac
     8641 ttt
//
