LOCUS       sequence-446484-       19799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single 
            intron-containing Cas9, EC1 promoter, kanamycin and red fluorescent 
            seed selection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19799)
  TITLE     Takeuchi CRISPR/Cas9 Plasmids for multi-gene knockout in Arabidopsis
REFERENCE   2  (bases 1 to 19799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHEE-R-kan9si
FEATURES             Location/Qualifiers
     source          1..19799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             531..1238
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     944..963
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2181..2972
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        3124..3199
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             5121..5186
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5193..5213
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5238..9338
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5238..9338
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             9251..9259
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9339..9386
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(10032..10048)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10032..10048)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10045..10067)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10056..10072
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10080..10086,10087..10104,10105..10110))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10125..10146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10337..11013
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10950..10970
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11076..11873
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11133..11152)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11743..11762
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11930..12104
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12182..12206
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12631..13425
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14850..15438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15339..15358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15592..15609
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15624..15764
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15690..15709)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15850..15872
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      16108..16302
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16368..17441)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17870..18499)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagtttc ttgaaaccaa attaacatag
       61 ggtttttatt taaataaaag tttaaccttc tttttaaaaa attgttcata gtgtcatgtc
      121 agaacaagag ctacaaatca cacatagcat gcataagcgg agctatgatg agtggtattg
      181 ttttgttcgt cacttgtcac tcttttccaa cacataatcc cgacaacaac gtaagagcat
      241 ctctctctct ccacacacac tcatgcatgc atgcattctt acacgtgatt gccatgcaaa
      301 tctcctttct cacctataaa tacaaaccaa cccttcacta cactcttcac tcaaaccaaa
      361 acaagaaaac atacacaaat agcaaaacaa gggcgaattc tcaacacaac atatacaaaa
      421 caaacgaatc tcaagcaatc aagcattcta cttctattgc agcaatttaa atcatttctt
      481 ttaaagcaaa agcaattttc tgaaaatttt caccatttac gaacgatagc atggtgagca
      541 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      601 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      661 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      721 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      781 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      841 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      901 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
      961 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1021 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1081 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1141 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1201 gccgccactc caccggcggc atggacgagc tgtacaagaa ggacgagctg taagcgccgc
     1261 ccggctgcat cgctgaaatc accagtctct ctctacaaat ctatctctct ctataataat
     1321 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     1381 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     1441 ctaattccta aaaccaaaat ccagtggtac caagcttcga cttgccttcc gcacaataca
     1501 tcatttcttc ttagcttttt ttcttcttct tcgttcatac agtttttttt tgtttatcag
     1561 cttacatttt cttgaaccgt agctttcgtt ttcttctttt taactttcca ttcggagttt
     1621 ttgtatcttg tttcatagtt tgtcccagga ttagaatgat taggcatcga accttcaaga
     1681 atttgattga ataaaacatc ttcattctta agatatgaag ataatcttca aaaggcccct
     1741 gggaatctga aagaagagaa gcaggcccat ttatatggga aagaacaata gtatttctta
     1801 tataggccca tttaagttga aaacaatctt caaaagtccc acatcgctta gataagaaaa
     1861 cgaagctgag tttatataca gctagagtcg aagtagtgat tgggagacca acccagtgga
     1921 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     1981 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2041 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2101 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2161 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     2221 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2281 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2341 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2401 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2461 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2521 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2581 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2641 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2701 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2761 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2821 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2881 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     2941 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     3001 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     3061 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccggtc
     3121 tcggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     3181 agtggcaccg agtcggtgct tttttttgca aaattttcca gatcgatttc ttcttcctct
     3241 gttcttcggc gttcaatttc tggggttttc tcttcgtttt ctgtaactga aacctaaaat
     3301 ttgacctaaa aaaaatctca aataatatga ttcagtggtt ttgtactttt cagttagttg
     3361 agttttgcag ttccgatgag ataaaccaat accatggtta tactagtgaa taaaagcatt
     3421 tgcgtttggt ttatcattgc gtttatacaa ggacagagat ccactgagct ggaatagctt
     3481 aaaaccatta tcagaacaaa ataaaccatt ttttgttaag aatcagagca tagtaaacaa
     3541 cagaaacaac ctaagagagg taacttgtcc aagaagatag ctaattatat ctattttata
     3601 aaagttatca tagtttgtaa gtcacaaaag atgcaaataa cagagaaact aggagacttg
     3661 agaatataca ttcttgtata tttgtattcg agattgtgaa aatttgacca taagtttaaa
     3721 ttcttaaaaa gatatatctg atctaggtga tggttataga ctgtaatttt accacatgtt
     3781 taatgatgga tagtgacaca catgacacat cgacaacact atagcatctt atttagatta
     3841 caacatgaaa tttttctgta atacatgtct ttgtacataa tttaaaagta attcctaaga
     3901 aatatattta tacaaggagt ttaaagaaaa catagcataa agttcaatga gtagtaaaaa
     3961 ccatatacag tatatagcat aaagttcaat gagtttatta caaaagcatt ggttcacttt
     4021 ctgtaacacg acgttaaacc ttcgtctcca ataggagcgc tactgattca acatgccaat
     4081 atatactaaa tacgtttcta cagtcaaatg ctttaacgtt tcatgattaa gtgactattt
     4141 accgtcaatc ctttcccatt cctcccacta atccaacttt ttaattactc ttaaatcacc
     4201 actaagctag taacgcctat catgaattag ctctactaaa tctagcaacc tttcaaattt
     4261 gcagtattgc aggtgtctct gtgtctttaa aatagttgcc ttatgatttc ttcggtttca
     4321 agatgatcaa atagttatag atttcatgct cacacatgct cattagatgt gtacatactt
     4381 tacttaccca aatctatttt ctcgcaaaga ttttgatggt aaagctgatt tggttctatt
     4441 gaactaaatc aaacgagttt cagactgagt gattctaatc cggcccatta gcccctaaac
     4501 agacccacta attacgcagc ttttaataga gtaattacac ctagtttacc cactaaacca
     4561 ctaagcacta attatctcac aatctaatga gcttccctcg taattacttg ggctttcact
     4621 ctaccattta tttgtaacag tcaagtctct actgtctcta tataaactct ctaaagttaa
     4681 cacacaattc tcatcacaaa caaatcaacc aaagcaactt ctactctttc ttctttcgac
     4741 cttatcaatc tgttgagaaa tctagatgca gccttctctt aaacgtatga aaattgagcc
     4801 atcttctcaa aaggtaaatt tctgtgttcc ttattctctc aaaatcttcg attttgtttt
     4861 cgttcgatcc caatttcgta tatgttcttt ggtttagatt ctgttaatct tagatcgaag
     4921 acgattttct gggtttgatc gttagatatc atcttaattc tcgattaggg tttcatagat
     4981 atcatccgat ttgttcaaat aatttgagtt ttgtcgaata attactcttc gatttgtgat
     5041 ttctatctag atctggtgtt agtttctagt ttgtgcgatc gaatttgtcg attaatctga
     5101 gtttttctga ttaacagatg gattacaagg accacgacgg ggattacaag gaccacgaca
     5161 ttgattacaa ggatgatgat gacaagatgg ctccgaagaa gaagaggaag gttggcatcc
     5221 acggggtgcc agctgctgac aagaagtact cgatcggcct cgatattggg actaactctg
     5281 ttggctgggc cgtgatcacc gacgagtaca aggtgccctc aaagaagttc aaggtcctgg
     5341 gcaacaccga tcggcattcc atcaagaaga atctcattgg cgctctcctg ttcgacagcg
     5401 gcgagacggc tgaggctacg cggctcaagc gcaccgcccg caggcggtac acgcgcagga
     5461 agaatcgcat ctgctacctg caggagattt tctccaacga gatggcgaag gttgacgatt
     5521 ctttcttcca caggctggag gagtcattcc tcgtggagga ggataagaag cacgagcggc
     5581 atccaatctt cggcaacatt gtcgacgagg ttgcctacca cgagaagtac cctacgatct
     5641 accatctgcg gaagaagctc gtggactcca cagataaggc ggacctccgc ctgatctacc
     5701 tcgctctggc ccacatgatt aagttcaggg gccatttcct gatcgagggg gatctcaacc
     5761 cggacaatag cgatgttgac aagctgttca tccagctcgt gcagacgtac aaccagctct
     5821 tcgaggagaa ccccattaat gcgtcaggcg tcgacgcgaa ggctatcctg tccgctaggc
     5881 tctcgaagtc tcggcgcctc gagaacctga tcgcccagct gccgggcgag aagaagaacg
     5941 gcctgttcgg gaatctcatt gcgctcagcc tggggctcac gcccaacttc aagtcgaatt
     6001 tcgatctcgc tgaggacgcc aagctgcagc tctccaagga cacatacgac gatgacctgg
     6061 ataacctcct ggcccagatc ggcgatcagt acgcggacct gttcctcgct gccaagaatc
     6121 tgtcggacgc catcctcctg tctgatattc tcagggtgaa caccgagatt acgaaggctc
     6181 cgctctcagc ctccatgatc aagcgctacg acgagcacca tcaggatctg accctcctga
     6241 aggcgctggt caggcagcag ctccccgaga agtacaagga gatcttcttc gatcagtcga
     6301 agaacggcta cgctgggtac attgacggcg gggcctctca ggaggagttc tacaagttca
     6361 tcaagccgat tctggagaag atggacggca cggaggagct gctggtgaag ctcaatcgcg
     6421 aggacctcct gaggaagcag cggacattcg ataacggcag catcccacac cagattcatc
     6481 tcggggagct gcacgctatc ctgaggaggc aggaggactt ctaccctttc ctcaaggata
     6541 accgcgagaa gatcgagaag attctgactt tcaggatccc gtactacgtc ggcccactcg
     6601 ctaggggcaa ctcccgcttc gcttggatga cccgcaagtc agaggagacg atcacgccgt
     6661 ggaacttcga ggaggtggtc gacaagggcg ctagcgctca gtcgttcatc gagaggatga
     6721 cgaatttcga caagaacctg ccaaatgaga aggtgctccc taagcactcg ctcctgtacg
     6781 agtacttcac agtctacaac gagctgacta aggtgaagta tgtgaccgag ggcatgagga
     6841 agccggcttt cctgtctggg gagcagaaga aggccatcgt ggacctcctg ttcaagacca
     6901 accggaaggt cacggttaag cagctcaagg aggactactt caagaagatt gagtgcttcg
     6961 attcggtcga gatctctggc gttgaggacc gcttcaacgc ctccctgggg acctaccacg
     7021 atctcctgaa gatcattaag gataaggact tcctggacaa cgaggagaat gaggatatcc
     7081 tcgaggacat tgtgctgaca ctcactctgt tcgaggaccg ggagatgatc gaggagcgcc
     7141 tgaagactta cgcccatctc ttcgatgaca aggtcatgaa gcagctcaag aggaggaggt
     7201 acaccggctg ggggaggctg agcaggaagc tcatcaacgg cattcgggac aagcagtccg
     7261 ggaagacgat cctcgacttc ctgaagagcg atggcttcgc gaaccgcaat ttcatgcagc
     7321 tgattcacga tgacagcctc acattcaagg aggatatcca gaaggctcag gtgagcggcc
     7381 agggggactc gctgcacgag catatcgcga acctcgctgg ctcgccagct atcaagaagg
     7441 ggattctgca gaccgtgaag gttgtggacg agctggtgaa ggtcatgggc aggcacaagc
     7501 ctgagaacat cgtcattgag atggcccggg agaatcagac cacgcagaag ggccagaaga
     7561 actcacgcga gaggatgaag aggatcgagg agggcattaa ggagctgggg tcccagatcc
     7621 tcaaggagca cccggtggag aacacgcagc tgcagaatga gaagctctac ctgtactacc
     7681 tccagaatgg ccgcgatatg tatgtggacc aggagctgga tattaacagg ctcagcgatt
     7741 acgacgtcga tcatatcgtt ccacagtcat tcctgaagga tgactccatt gacaacaagg
     7801 tcctcaccag gtcggacaag aaccggggca agtctgataa tgttccttca gaggaggtcg
     7861 ttaagaagat gaagaactac tggcgccagc tcctgaatgc caagctgatc acgcagcgga
     7921 agttcgataa cctcacaaag gctgagaggg gcgggctctc tgagctggac aaggcgggct
     7981 tcatcaagag gcagctggtc gagacacggc agatcactaa gcacgttgcg cagattctcg
     8041 actcacggat gaacactaag tacgatgaga atgacaagct gatccgcgag gtgaaggtca
     8101 tcaccctgaa gtcaaagctc gtctccgact tcaggaagga tttccagttc tacaaggttc
     8161 gggagatcaa caattaccac catgcccatg acgcgtacct gaacgcggtg gtcggcacag
     8221 ctctgatcaa gaagtaccca aagctcgaga gcgagttcgt gtacggggac tacaaggttt
     8281 acgatgtgag gaagatgatc gccaagtcgg agcaggagat tggcaaggct accgccaagt
     8341 acttcttcta ctctaacatt atgaatttct tcaagacaga gatcactctg gccaatggcg
     8401 agatccggaa gcgccccctc atcgagacga acggcgagac gggggagatc gtgtgggaca
     8461 agggcaggga tttcgcgacc gtcaggaagg ttctctccat gccacaagtg aatatcgtca
     8521 agaagacaga ggtccagact ggcgggttct ctaaggagtc aattctgcct aagcggaaca
     8581 gcgacaagct catcgcccgc aagaaggact gggatccgaa gaagtacggc gggttcgaca
     8641 gccccactgt ggcctactcg gtcctggttg tggcgaaggt tgagaagggc aagtccaaga
     8701 agctcaagag cgtgaaggag ctgctgggga tcacgattat ggagcgctcc agcttcgaga
     8761 agaacccgat cgatttcctg gaggcgaagg gctacaagga ggtgaagaag gacctgatca
     8821 ttaagctccc caagtactca ctcttcgagc tggagaacgg caggaagcgg atgctggctt
     8881 ccgctggcga gctgcagaag gggaacgagc tggctctgcc gtccaagtat gtgaacttcc
     8941 tctacctggc ctcccactac gagaagctca agggcagccc cgaggacaac gagcagaagc
     9001 agctgttcgt cgagcagcac aagcattacc tcgacgagat cattgagcag atttccgagt
     9061 tctccaagcg cgtgatcctg gccgacgcga atctggataa ggtcctctcc gcgtacaaca
     9121 agcaccgcga caagccaatc agggagcagg ctgagaatat cattcatctc ttcaccctga
     9181 cgaacctcgg cgcccctgct gctttcaagt acttcgacac aactatcgat cgcaagaggt
     9241 acacaagcac taaggaggtc ctggacgcga ccctcatcca ccagtcgatt accggcctct
     9301 acgagacgcg catcgacctg tctcagctcg ggggcgacaa gcggccagcg gcgacgaaga
     9361 aggcggggca ggcgaagaag aagaagtgag ctcagagctt tcgttcgtat catcggtttc
     9421 gacaacgttc gtcaagttca atgcatcagt ttcattgcgc acacaccaga atcctactga
     9481 gtttgagtat tatggcattg ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat
     9541 ttactgtgtt ttttattcgg ttttcgctat cgaactgtga aatggaaatg gatggagaag
     9601 agttaatgaa tgatatggtc cttttgttca ttctcaaatt aatattattt gttttttctc
     9661 ttatttgttg tgtgttgaat ttgaaattat aagagatatg caaacatttt gttttgagta
     9721 aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat atgaggagta aaacacttgt
     9781 agttgtacca ttatgcttat tcactaggca acaaatatat tttcagacct agaaaagctg
     9841 caaatgttac tgaatacaag tatgtcctct tgtgttttag acatttatga actttccttt
     9901 atgtaatttt ccagaatcct tgtcagattc taatcattgc tttataatta tagttatact
     9961 catggatttg tagttgagta tgaaaatatt ttttaatgca ttttatgact tgccaattgg
    10021 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    10081 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    10141 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    10201 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattggcta gagcagcttg
    10261 ccaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag
    10321 aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat
    10381 tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct
    10441 acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg
    10501 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
    10561 cgtcttcaaa gcaagtggat tgatgtgaac atggtggagc acgacactct cgtctactcc
    10621 aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
    10681 gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg
    10741 acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc
    10801 gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
    10861 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc
    10921 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
    10981 ggaagttcat ttcatttgga gaggacacgc tgaaatcacc agtctctctc tacaaatcta
    11041 tctctctcga gctttcgcag atctgtcgat cgaccatggg gattgaacaa gatggattgc
    11101 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
    11161 caatcggctg ctctgatgcc gccgtgtttc ggctgtcagc gcaggggcgc ccggttcttt
    11221 ttgtcaagac cgacctgtcc ggtgccctga atgaactcca ggacgaggca gcgcggctat
    11281 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
    11341 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
    11401 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
    11461 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
    11521 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
    11581 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgacac
    11641 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
    11701 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
    11761 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
    11821 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
    11881 tctggggttc ggatcgatcc tctagctaga gtcgatcgac aagctcgagt ttctccataa
    11941 taatgtgtga gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg
    12001 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
    12061 atttctaatt cctaaaacca aaatccagta ctaaaatcca gatcccccga attaattcgg
    12121 cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt
    12181 tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg
    12241 gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag
    12301 ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta
    12361 agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg
    12421 acagagcgtt gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt
    12481 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
    12541 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
    12601 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
    12661 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
    12721 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
    12781 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
    12841 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
    12901 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
    12961 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
    13021 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
    13081 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
    13141 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
    13201 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
    13261 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
    13321 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
    13381 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agaatgcatg
    13441 accaaaatcc cttaacgtga gttttcgttc cactgagcgc tgagagatcc cctcataatt
    13501 tccccaaagc gtaaccatgt gtgaataaat tttgagctag tagggttgca gccacgagta
    13561 agtcttccct tgttattgtg tagccagaat gccgcaaaac ttccatgcct aagcgaactg
    13621 ttgagagtac gtttcgattt ctgactgtgt tagcctggaa gtgcttgtcc caaccttgtt
    13681 tctgagcatg aacgcccgca agccaacatg ttagttgaag catcagggcg attagcagca
    13741 tgatatcaaa acgctctgag ctgctcgttc ggctatggcg taggcctagt ccgtaggcag
    13801 gacttttcaa gtctcggaag gtttcttcaa tctgcattcg cttcgaatag atattaacaa
    13861 gttgtttggg tgttcgaatt tcaacaggta agttagttgc tagaacccat ggctcctttg
    13921 ccgacgctga gtagatttta ggtgacgggt ggtgacaatg agtccgtgtc gagcgctgat
    13981 tttttcggcc tttagagcga gatttataca atagaatttg gcatgagatt ggattgcttt
    14041 tagtcagcct cttatagcct aaagtctttg agtgactaga tgacatatca tgtaagttgc
    14101 tgataggttt ccagttttcc gctcctaggt ctgcatattg tacttttcct cttactcgac
    14161 ttaaccagta ccaacccagc ttctcaacgg atttatacca tggcacttta aagccagcat
    14221 cactgacaat gagcggtgtg gtgttactcg gtagaatgct cgcaaggtcg gctagaaatt
    14281 ggtcatgagc tttctttgaa cattgctctg aaagcgggaa cgctttctca taaagagtaa
    14341 cagaacgacc gtgtagtgcg actgaagctc gcaataccat aagtcgtttt tgctcacgaa
    14401 tatcagacca gtcaacaagt acaatgggca tcgtattgcc cgaacagata aagctagcat
    14461 gccaacggta tacagcgagt cgctctttgt ggaggtgacg attacctaac aatcggtcga
    14521 ttcgtttgat gttatgtttt gttctcgctt tggttggcag gttacggcca agttcggtaa
    14581 gagtgagagt tttacagtca agtaatgcgt ggcaagccaa cgttaagctg ttgagtcgtt
    14641 ttaagtgtaa ttcggggcag aattggtaaa gagagtcgtg taaaatatcg agttcgcaca
    14701 tcttgttgtc tgattattga tttttcgcga aaccatttga tcatatgaca agatgtgtat
    14761 ccaccttaac ttaatgattt ttaccaaaat cattagggga ttcatcagca ctgagcgtca
    14821 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    14881 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    14941 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
    15001 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    15061 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    15121 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    15181 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    15241 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    15301 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    15361 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    15421 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    15481 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    15541 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    15601 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
    15661 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
    15721 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
    15781 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
    15841 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
    15901 ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc
    15961 cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc
    16021 cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc
    16081 tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta
    16141 ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact
    16201 tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc
    16261 ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaacag accttttcga
    16321 cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg
    16381 gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc
    16441 gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg
    16501 aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac
    16561 catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg
    16621 ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct
    16681 gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt
    16741 tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg
    16801 cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg
    16861 caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt
    16921 acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat
    16981 cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca
    17041 ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg
    17101 atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg
    17161 ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg
    17221 tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct
    17281 ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg
    17341 cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc
    17401 gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc tcgggcttgg
    17461 gggttccagt gccattgcag ggccggcagg caacccagcc gcttacgcct ggccaaccgc
    17521 ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca
    17581 tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct catttactct
    17641 ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg
    17701 tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct
    17761 ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg
    17821 gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt
    17881 ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg
    17941 attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac
    18001 gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc
    18061 gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct
    18121 caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg
    18181 gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg
    18241 cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt
    18301 caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc
    18361 aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt
    18421 gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa
    18481 gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat
    18541 atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg
    18601 gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga
    18661 caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac
    18721 gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg
    18781 gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg
    18841 gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc
    18901 gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt
    18961 gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc
    19021 tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg
    19081 cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac
    19141 acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta
    19201 cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg
    19261 cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc
    19321 atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg
    19381 gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg
    19441 acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg
    19501 ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa
    19561 aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa
    19621 gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa
    19681 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
    19741 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttag
//
