LOCUS       sequence-446485-       19485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single 
            intron-containing Cas9, EC1 promoter, bialaphos and red fluorescent 
            seed selection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19485)
  TITLE     Takeuchi CRISPR/Cas9 Plasmids for multi-gene knockout in Arabidopsis
REFERENCE   2  (bases 1 to 19485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHEE-R-bar9si
FEATURES             Location/Qualifiers
     source          1..19485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             531..1238
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     944..963
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2181..2972
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        3124..3199
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             5121..5186
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5193..5213
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5238..9338
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5238..9338
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             9251..9259
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9339..9386
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(10032..10048)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10032..10048)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10045..10067)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10056..10072
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10080..10086,10087..10104,10105..10110))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10125..10146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10337..11013
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10950..10970
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11058..11609
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    11616..11790
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11868..11892
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12317..13111
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14536..15124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15025..15044
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15278..15295
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15310..15450
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15376..15395)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15536..15558
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      15794..15988
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16054..17127)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17556..18185)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagtttc ttgaaaccaa attaacatag
       61 ggtttttatt taaataaaag tttaaccttc tttttaaaaa attgttcata gtgtcatgtc
      121 agaacaagag ctacaaatca cacatagcat gcataagcgg agctatgatg agtggtattg
      181 ttttgttcgt cacttgtcac tcttttccaa cacataatcc cgacaacaac gtaagagcat
      241 ctctctctct ccacacacac tcatgcatgc atgcattctt acacgtgatt gccatgcaaa
      301 tctcctttct cacctataaa tacaaaccaa cccttcacta cactcttcac tcaaaccaaa
      361 acaagaaaac atacacaaat agcaaaacaa gggcgaattc tcaacacaac atatacaaaa
      421 caaacgaatc tcaagcaatc aagcattcta cttctattgc agcaatttaa atcatttctt
      481 ttaaagcaaa agcaattttc tgaaaatttt caccatttac gaacgatagc atggtgagca
      541 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      601 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      661 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      721 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      781 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      841 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      901 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
      961 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1021 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1081 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1141 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1201 gccgccactc caccggcggc atggacgagc tgtacaagaa ggacgagctg taagcgccgc
     1261 ccggctgcat cgctgaaatc accagtctct ctctacaaat ctatctctct ctataataat
     1321 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     1381 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     1441 ctaattccta aaaccaaaat ccagtggtac caagcttcga cttgccttcc gcacaataca
     1501 tcatttcttc ttagcttttt ttcttcttct tcgttcatac agtttttttt tgtttatcag
     1561 cttacatttt cttgaaccgt agctttcgtt ttcttctttt taactttcca ttcggagttt
     1621 ttgtatcttg tttcatagtt tgtcccagga ttagaatgat taggcatcga accttcaaga
     1681 atttgattga ataaaacatc ttcattctta agatatgaag ataatcttca aaaggcccct
     1741 gggaatctga aagaagagaa gcaggcccat ttatatggga aagaacaata gtatttctta
     1801 tataggccca tttaagttga aaacaatctt caaaagtccc acatcgctta gataagaaaa
     1861 cgaagctgag tttatataca gctagagtcg aagtagtgat tgggagacca acccagtgga
     1921 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     1981 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2041 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2101 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2161 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     2221 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2281 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2341 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2401 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2461 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2521 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2581 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2641 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2701 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2761 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2821 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2881 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     2941 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     3001 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     3061 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccggtc
     3121 tcggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     3181 agtggcaccg agtcggtgct tttttttgca aaattttcca gatcgatttc ttcttcctct
     3241 gttcttcggc gttcaatttc tggggttttc tcttcgtttt ctgtaactga aacctaaaat
     3301 ttgacctaaa aaaaatctca aataatatga ttcagtggtt ttgtactttt cagttagttg
     3361 agttttgcag ttccgatgag ataaaccaat accatggtta tactagtgaa taaaagcatt
     3421 tgcgtttggt ttatcattgc gtttatacaa ggacagagat ccactgagct ggaatagctt
     3481 aaaaccatta tcagaacaaa ataaaccatt ttttgttaag aatcagagca tagtaaacaa
     3541 cagaaacaac ctaagagagg taacttgtcc aagaagatag ctaattatat ctattttata
     3601 aaagttatca tagtttgtaa gtcacaaaag atgcaaataa cagagaaact aggagacttg
     3661 agaatataca ttcttgtata tttgtattcg agattgtgaa aatttgacca taagtttaaa
     3721 ttcttaaaaa gatatatctg atctaggtga tggttataga ctgtaatttt accacatgtt
     3781 taatgatgga tagtgacaca catgacacat cgacaacact atagcatctt atttagatta
     3841 caacatgaaa tttttctgta atacatgtct ttgtacataa tttaaaagta attcctaaga
     3901 aatatattta tacaaggagt ttaaagaaaa catagcataa agttcaatga gtagtaaaaa
     3961 ccatatacag tatatagcat aaagttcaat gagtttatta caaaagcatt ggttcacttt
     4021 ctgtaacacg acgttaaacc ttcgtctcca ataggagcgc tactgattca acatgccaat
     4081 atatactaaa tacgtttcta cagtcaaatg ctttaacgtt tcatgattaa gtgactattt
     4141 accgtcaatc ctttcccatt cctcccacta atccaacttt ttaattactc ttaaatcacc
     4201 actaagctag taacgcctat catgaattag ctctactaaa tctagcaacc tttcaaattt
     4261 gcagtattgc aggtgtctct gtgtctttaa aatagttgcc ttatgatttc ttcggtttca
     4321 agatgatcaa atagttatag atttcatgct cacacatgct cattagatgt gtacatactt
     4381 tacttaccca aatctatttt ctcgcaaaga ttttgatggt aaagctgatt tggttctatt
     4441 gaactaaatc aaacgagttt cagactgagt gattctaatc cggcccatta gcccctaaac
     4501 agacccacta attacgcagc ttttaataga gtaattacac ctagtttacc cactaaacca
     4561 ctaagcacta attatctcac aatctaatga gcttccctcg taattacttg ggctttcact
     4621 ctaccattta tttgtaacag tcaagtctct actgtctcta tataaactct ctaaagttaa
     4681 cacacaattc tcatcacaaa caaatcaacc aaagcaactt ctactctttc ttctttcgac
     4741 cttatcaatc tgttgagaaa tctagatgca gccttctctt aaacgtatga aaattgagcc
     4801 atcttctcaa aaggtaaatt tctgtgttcc ttattctctc aaaatcttcg attttgtttt
     4861 cgttcgatcc caatttcgta tatgttcttt ggtttagatt ctgttaatct tagatcgaag
     4921 acgattttct gggtttgatc gttagatatc atcttaattc tcgattaggg tttcatagat
     4981 atcatccgat ttgttcaaat aatttgagtt ttgtcgaata attactcttc gatttgtgat
     5041 ttctatctag atctggtgtt agtttctagt ttgtgcgatc gaatttgtcg attaatctga
     5101 gtttttctga ttaacagatg gattacaagg accacgacgg ggattacaag gaccacgaca
     5161 ttgattacaa ggatgatgat gacaagatgg ctccgaagaa gaagaggaag gttggcatcc
     5221 acggggtgcc agctgctgac aagaagtact cgatcggcct cgatattggg actaactctg
     5281 ttggctgggc cgtgatcacc gacgagtaca aggtgccctc aaagaagttc aaggtcctgg
     5341 gcaacaccga tcggcattcc atcaagaaga atctcattgg cgctctcctg ttcgacagcg
     5401 gcgagacggc tgaggctacg cggctcaagc gcaccgcccg caggcggtac acgcgcagga
     5461 agaatcgcat ctgctacctg caggagattt tctccaacga gatggcgaag gttgacgatt
     5521 ctttcttcca caggctggag gagtcattcc tcgtggagga ggataagaag cacgagcggc
     5581 atccaatctt cggcaacatt gtcgacgagg ttgcctacca cgagaagtac cctacgatct
     5641 accatctgcg gaagaagctc gtggactcca cagataaggc ggacctccgc ctgatctacc
     5701 tcgctctggc ccacatgatt aagttcaggg gccatttcct gatcgagggg gatctcaacc
     5761 cggacaatag cgatgttgac aagctgttca tccagctcgt gcagacgtac aaccagctct
     5821 tcgaggagaa ccccattaat gcgtcaggcg tcgacgcgaa ggctatcctg tccgctaggc
     5881 tctcgaagtc tcggcgcctc gagaacctga tcgcccagct gccgggcgag aagaagaacg
     5941 gcctgttcgg gaatctcatt gcgctcagcc tggggctcac gcccaacttc aagtcgaatt
     6001 tcgatctcgc tgaggacgcc aagctgcagc tctccaagga cacatacgac gatgacctgg
     6061 ataacctcct ggcccagatc ggcgatcagt acgcggacct gttcctcgct gccaagaatc
     6121 tgtcggacgc catcctcctg tctgatattc tcagggtgaa caccgagatt acgaaggctc
     6181 cgctctcagc ctccatgatc aagcgctacg acgagcacca tcaggatctg accctcctga
     6241 aggcgctggt caggcagcag ctccccgaga agtacaagga gatcttcttc gatcagtcga
     6301 agaacggcta cgctgggtac attgacggcg gggcctctca ggaggagttc tacaagttca
     6361 tcaagccgat tctggagaag atggacggca cggaggagct gctggtgaag ctcaatcgcg
     6421 aggacctcct gaggaagcag cggacattcg ataacggcag catcccacac cagattcatc
     6481 tcggggagct gcacgctatc ctgaggaggc aggaggactt ctaccctttc ctcaaggata
     6541 accgcgagaa gatcgagaag attctgactt tcaggatccc gtactacgtc ggcccactcg
     6601 ctaggggcaa ctcccgcttc gcttggatga cccgcaagtc agaggagacg atcacgccgt
     6661 ggaacttcga ggaggtggtc gacaagggcg ctagcgctca gtcgttcatc gagaggatga
     6721 cgaatttcga caagaacctg ccaaatgaga aggtgctccc taagcactcg ctcctgtacg
     6781 agtacttcac agtctacaac gagctgacta aggtgaagta tgtgaccgag ggcatgagga
     6841 agccggcttt cctgtctggg gagcagaaga aggccatcgt ggacctcctg ttcaagacca
     6901 accggaaggt cacggttaag cagctcaagg aggactactt caagaagatt gagtgcttcg
     6961 attcggtcga gatctctggc gttgaggacc gcttcaacgc ctccctgggg acctaccacg
     7021 atctcctgaa gatcattaag gataaggact tcctggacaa cgaggagaat gaggatatcc
     7081 tcgaggacat tgtgctgaca ctcactctgt tcgaggaccg ggagatgatc gaggagcgcc
     7141 tgaagactta cgcccatctc ttcgatgaca aggtcatgaa gcagctcaag aggaggaggt
     7201 acaccggctg ggggaggctg agcaggaagc tcatcaacgg cattcgggac aagcagtccg
     7261 ggaagacgat cctcgacttc ctgaagagcg atggcttcgc gaaccgcaat ttcatgcagc
     7321 tgattcacga tgacagcctc acattcaagg aggatatcca gaaggctcag gtgagcggcc
     7381 agggggactc gctgcacgag catatcgcga acctcgctgg ctcgccagct atcaagaagg
     7441 ggattctgca gaccgtgaag gttgtggacg agctggtgaa ggtcatgggc aggcacaagc
     7501 ctgagaacat cgtcattgag atggcccggg agaatcagac cacgcagaag ggccagaaga
     7561 actcacgcga gaggatgaag aggatcgagg agggcattaa ggagctgggg tcccagatcc
     7621 tcaaggagca cccggtggag aacacgcagc tgcagaatga gaagctctac ctgtactacc
     7681 tccagaatgg ccgcgatatg tatgtggacc aggagctgga tattaacagg ctcagcgatt
     7741 acgacgtcga tcatatcgtt ccacagtcat tcctgaagga tgactccatt gacaacaagg
     7801 tcctcaccag gtcggacaag aaccggggca agtctgataa tgttccttca gaggaggtcg
     7861 ttaagaagat gaagaactac tggcgccagc tcctgaatgc caagctgatc acgcagcgga
     7921 agttcgataa cctcacaaag gctgagaggg gcgggctctc tgagctggac aaggcgggct
     7981 tcatcaagag gcagctggtc gagacacggc agatcactaa gcacgttgcg cagattctcg
     8041 actcacggat gaacactaag tacgatgaga atgacaagct gatccgcgag gtgaaggtca
     8101 tcaccctgaa gtcaaagctc gtctccgact tcaggaagga tttccagttc tacaaggttc
     8161 gggagatcaa caattaccac catgcccatg acgcgtacct gaacgcggtg gtcggcacag
     8221 ctctgatcaa gaagtaccca aagctcgaga gcgagttcgt gtacggggac tacaaggttt
     8281 acgatgtgag gaagatgatc gccaagtcgg agcaggagat tggcaaggct accgccaagt
     8341 acttcttcta ctctaacatt atgaatttct tcaagacaga gatcactctg gccaatggcg
     8401 agatccggaa gcgccccctc atcgagacga acggcgagac gggggagatc gtgtgggaca
     8461 agggcaggga tttcgcgacc gtcaggaagg ttctctccat gccacaagtg aatatcgtca
     8521 agaagacaga ggtccagact ggcgggttct ctaaggagtc aattctgcct aagcggaaca
     8581 gcgacaagct catcgcccgc aagaaggact gggatccgaa gaagtacggc gggttcgaca
     8641 gccccactgt ggcctactcg gtcctggttg tggcgaaggt tgagaagggc aagtccaaga
     8701 agctcaagag cgtgaaggag ctgctgggga tcacgattat ggagcgctcc agcttcgaga
     8761 agaacccgat cgatttcctg gaggcgaagg gctacaagga ggtgaagaag gacctgatca
     8821 ttaagctccc caagtactca ctcttcgagc tggagaacgg caggaagcgg atgctggctt
     8881 ccgctggcga gctgcagaag gggaacgagc tggctctgcc gtccaagtat gtgaacttcc
     8941 tctacctggc ctcccactac gagaagctca agggcagccc cgaggacaac gagcagaagc
     9001 agctgttcgt cgagcagcac aagcattacc tcgacgagat cattgagcag atttccgagt
     9061 tctccaagcg cgtgatcctg gccgacgcga atctggataa ggtcctctcc gcgtacaaca
     9121 agcaccgcga caagccaatc agggagcagg ctgagaatat cattcatctc ttcaccctga
     9181 cgaacctcgg cgcccctgct gctttcaagt acttcgacac aactatcgat cgcaagaggt
     9241 acacaagcac taaggaggtc ctggacgcga ccctcatcca ccagtcgatt accggcctct
     9301 acgagacgcg catcgacctg tctcagctcg ggggcgacaa gcggccagcg gcgacgaaga
     9361 aggcggggca ggcgaagaag aagaagtgag ctcagagctt tcgttcgtat catcggtttc
     9421 gacaacgttc gtcaagttca atgcatcagt ttcattgcgc acacaccaga atcctactga
     9481 gtttgagtat tatggcattg ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat
     9541 ttactgtgtt ttttattcgg ttttcgctat cgaactgtga aatggaaatg gatggagaag
     9601 agttaatgaa tgatatggtc cttttgttca ttctcaaatt aatattattt gttttttctc
     9661 ttatttgttg tgtgttgaat ttgaaattat aagagatatg caaacatttt gttttgagta
     9721 aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat atgaggagta aaacacttgt
     9781 agttgtacca ttatgcttat tcactaggca acaaatatat tttcagacct agaaaagctg
     9841 caaatgttac tgaatacaag tatgtcctct tgtgttttag acatttatga actttccttt
     9901 atgtaatttt ccagaatcct tgtcagattc taatcattgc tttataatta tagttatact
     9961 catggatttg tagttgagta tgaaaatatt ttttaatgca ttttatgact tgccaattgg
    10021 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    10081 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    10141 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    10201 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattggcta gagcagcttg
    10261 ccaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag
    10321 aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat
    10381 tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct
    10441 acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg
    10501 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
    10561 cgtcttcaaa gcaagtggat tgatgtgaac atggtggagc acgacactct cgtctactcc
    10621 aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
    10681 gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg
    10741 acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc
    10801 gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
    10861 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc
    10921 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
    10981 ggaagttcat ttcatttgga gaggacacgc tgaaatcacc agtctctctc tacaaatcta
    11041 tctctctcga gtctaccatg agcccagaac gacgcccggc cgacatccgc cgtgccaccg
    11101 aggcggacat gccggcggtc tgcaccatcg tcaaccacta catcgagaca agcacggtca
    11161 acttccgtac cgagccgcag gaaccgcagg agtggacgga cgacctcgtc cgtctgcggg
    11221 agcgctatcc ctggctcgtc gccgaggtgg acggcgaggt cgccggcatc gcctacgcgg
    11281 gcccctggaa ggcacgcaac gcctacgact ggacggccga gtcgaccgtg tacgtctccc
    11341 cccgccacca gcggacggga ctgggctcca cgctctacac ccacctgctg aagtccctgg
    11401 aggcacaggg cttcaagagc gtggtcgctg tcatcgggct gcccaacgac ccgagcgtgc
    11461 gcatgcacga ggcgctcgga tatgcccccc gcggcatgct gcgggcggcc ggcttcaagc
    11521 acgggaactg gcatgacgtg ggtttctggc agctggactt cagcctgccg gtaccgcccc
    11581 gtccggtcct gcccgtcacc gagatttgac tcgagtttct ccataataat gtgtgagtag
    11641 ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga
    11701 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
    11761 aaaccaaaat ccagtactaa aatccagatc ccccgaatta attcggcgtt aattcagtac
    11821 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
    11881 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
    11941 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
    12001 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
    12061 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
    12121 cctgtgatca ccgcggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg
    12181 aaaacaactt tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg
    12241 aattggagtt cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag
    12301 aggaaggaaa taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga
    12361 aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt
    12421 gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta
    12481 tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa
    12541 ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg
    12601 cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta
    12661 tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag
    12721 cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga
    12781 ttgcgaaaac tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt
    12841 aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa
    12901 catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc
    12961 ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga
    13021 acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa
    13081 atattatatt ttactggatg aattgtttta gtacctagaa tgcatgacca aaatccctta
    13141 acgtgagttt tcgttccact gagcgctgag agatcccctc ataatttccc caaagcgtaa
    13201 ccatgtgtga ataaattttg agctagtagg gttgcagcca cgagtaagtc ttcccttgtt
    13261 attgtgtagc cagaatgccg caaaacttcc atgcctaagc gaactgttga gagtacgttt
    13321 cgatttctga ctgtgttagc ctggaagtgc ttgtcccaac cttgtttctg agcatgaacg
    13381 cccgcaagcc aacatgttag ttgaagcatc agggcgatta gcagcatgat atcaaaacgc
    13441 tctgagctgc tcgttcggct atggcgtagg cctagtccgt aggcaggact tttcaagtct
    13501 cggaaggttt cttcaatctg cattcgcttc gaatagatat taacaagttg tttgggtgtt
    13561 cgaatttcaa caggtaagtt agttgctaga acccatggct cctttgccga cgctgagtag
    13621 attttaggtg acgggtggtg acaatgagtc cgtgtcgagc gctgattttt tcggccttta
    13681 gagcgagatt tatacaatag aatttggcat gagattggat tgcttttagt cagcctctta
    13741 tagcctaaag tctttgagtg actagatgac atatcatgta agttgctgat aggtttccag
    13801 ttttccgctc ctaggtctgc atattgtact tttcctctta ctcgacttaa ccagtaccaa
    13861 cccagcttct caacggattt ataccatggc actttaaagc cagcatcact gacaatgagc
    13921 ggtgtggtgt tactcggtag aatgctcgca aggtcggcta gaaattggtc atgagctttc
    13981 tttgaacatt gctctgaaag cgggaacgct ttctcataaa gagtaacaga acgaccgtgt
    14041 agtgcgactg aagctcgcaa taccataagt cgtttttgct cacgaatatc agaccagtca
    14101 acaagtacaa tgggcatcgt attgcccgaa cagataaagc tagcatgcca acggtataca
    14161 gcgagtcgct ctttgtggag gtgacgatta cctaacaatc ggtcgattcg tttgatgtta
    14221 tgttttgttc tcgctttggt tggcaggtta cggccaagtt cggtaagagt gagagtttta
    14281 cagtcaagta atgcgtggca agccaacgtt aagctgttga gtcgttttaa gtgtaattcg
    14341 gggcagaatt ggtaaagaga gtcgtgtaaa atatcgagtt cgcacatctt gttgtctgat
    14401 tattgatttt tcgcgaaacc atttgatcat atgacaagat gtgtatccac cttaacttaa
    14461 tgatttttac caaaatcatt aggggattca tcagcactga gcgtcagacc ccgtagaaaa
    14521 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    14581 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    14641 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
    14701 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    14761 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    14821 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    14881 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    14941 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    15001 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    15061 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    15121 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    15181 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    15241 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    15301 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
    15361 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
    15421 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
    15481 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
    15541 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg
    15601 atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc
    15661 tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
    15721 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg
    15781 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
    15841 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
    15901 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
    15961 tgggttccca atgtacgtgc tatccacagg aaacagacct tttcgacctt tttcccctgc
    16021 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
    16081 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
    16141 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
    16201 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
    16261 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
    16321 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
    16381 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
    16441 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
    16501 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
    16561 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
    16621 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
    16681 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
    16741 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
    16801 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
    16861 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
    16921 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
    16981 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
    17041 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
    17101 cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca
    17161 ttgcagggcc ggcaggcaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac
    17221 atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta
    17281 gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat
    17341 gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt
    17401 ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag
    17461 gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt
    17521 tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca
    17581 gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt
    17641 gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg
    17701 ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc
    17761 gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta
    17821 accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc
    17881 agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg
    17941 tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg
    18001 tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg
    18061 ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct
    18121 agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc
    18181 ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg
    18241 catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt
    18301 cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga
    18361 tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga
    18421 tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg
    18481 gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt
    18541 caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct
    18601 gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt
    18661 cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag
    18721 ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt
    18781 gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat
    18841 gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc
    18901 ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc
    18961 tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc
    19021 cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc
    19081 gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc
    19141 cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt
    19201 attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc
    19261 agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga
    19321 acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttgatcccg
    19381 aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
    19441 ccttttaaat atccgattat tctaataaac gctcttttct cttag
//
