LOCUS       sequence-446486-       20333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single 
            intron-containing Cas9, RPS5A promoter, hygromycin and red 
            fluorescent seed selection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20333)
  TITLE     Takeuchi CRISPR/Cas9 Plasmids for multi-gene knockout in Arabidopsis
REFERENCE   2  (bases 1 to 20333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHEE-R-hyg5A9si
FEATURES             Location/Qualifiers
     source          1..20333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             531..1238
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     944..963
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2181..2972
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        3124..3199
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             5428..5493
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5500..5520
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5545..9645
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5545..9645
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             9558..9566
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9646..9693
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(10350..10366)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10350..10366)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10363..10385)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10374..10390
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10398..10404,10405..10422,10423..10428))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10443..10464
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10655..11331
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     11268..11288
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11398..12423
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    12464..12638
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12716..12740
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             13165..13959
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      15384..15972
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15873..15892
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16126..16143
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    16158..16298
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(16224..16243)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     16384..16406
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      16642..16836
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16902..17975)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(18404..19033)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagtttc ttgaaaccaa attaacatag
       61 ggtttttatt taaataaaag tttaaccttc tttttaaaaa attgttcata gtgtcatgtc
      121 agaacaagag ctacaaatca cacatagcat gcataagcgg agctatgatg agtggtattg
      181 ttttgttcgt cacttgtcac tcttttccaa cacataatcc cgacaacaac gtaagagcat
      241 ctctctctct ccacacacac tcatgcatgc atgcattctt acacgtgatt gccatgcaaa
      301 tctcctttct cacctataaa tacaaaccaa cccttcacta cactcttcac tcaaaccaaa
      361 acaagaaaac atacacaaat agcaaaacaa gggcgaattc tcaacacaac atatacaaaa
      421 caaacgaatc tcaagcaatc aagcattcta cttctattgc agcaatttaa atcatttctt
      481 ttaaagcaaa agcaattttc tgaaaatttt caccatttac gaacgatagc atggtgagca
      541 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      601 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      661 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      721 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      781 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      841 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      901 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
      961 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1021 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1081 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1141 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1201 gccgccactc caccggcggc atggacgagc tgtacaagaa ggacgagctg taagcgccgc
     1261 ccggctgcat cgctgaaatc accagtctct ctctacaaat ctatctctct ctataataat
     1321 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     1381 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     1441 ctaattccta aaaccaaaat ccagtggtac caagcttcga cttgccttcc gcacaataca
     1501 tcatttcttc ttagcttttt ttcttcttct tcgttcatac agtttttttt tgtttatcag
     1561 cttacatttt cttgaaccgt agctttcgtt ttcttctttt taactttcca ttcggagttt
     1621 ttgtatcttg tttcatagtt tgtcccagga ttagaatgat taggcatcga accttcaaga
     1681 atttgattga ataaaacatc ttcattctta agatatgaag ataatcttca aaaggcccct
     1741 gggaatctga aagaagagaa gcaggcccat ttatatggga aagaacaata gtatttctta
     1801 tataggccca tttaagttga aaacaatctt caaaagtccc acatcgctta gataagaaaa
     1861 cgaagctgag tttatataca gctagagtcg aagtagtgat tgggagacca acccagtgga
     1921 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     1981 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2041 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2101 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2161 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     2221 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2281 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2341 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2401 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2461 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2521 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2581 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2641 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2701 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2761 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2821 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2881 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     2941 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     3001 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     3061 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccggtc
     3121 tcggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     3181 agtggcaccg agtcggtgct tttttttgca aaattttcca gatcgatttc ttcttcctct
     3241 gttcttcggc gttcaatttc tggggttttc tcttcgtttt ctgtaactga aacctaaaat
     3301 ttgacctaaa aaaaatctca aataatatga ttcagtggtt ttgtactttt cagttagttg
     3361 agttttgcag ttccgatgag ataaaccaat accatggtta tactagtctc aacttttgat
     3421 tcgctatttg cagtgcacct gtggcgttca tcacatcttt tgtgacactg tttgcactgg
     3481 tcattgctat tacaaaggac cttcctgatg ttgaaggaga tcgaaagtaa gtaactgcac
     3541 gcataaccat tttctttccg ctctttggct caatccattt gacagtcaaa gacaatgttt
     3601 aaccagctcc gtttgatata ttgtctttat gtgtttgttc aagcatgttt agttaatcat
     3661 gcctttgatt gatcttgaat aggttccaaa tatcaaccct ggcaacaaaa cttggagtga
     3721 gaaacattgc attcctcggt tctggacttc tgctagtaaa ttatgtttca gccatatcac
     3781 tagctttcta catgcctcag gtgaattcat ctatttccgt cttaactatt tcggttaatt
     3841 aaagcacgaa caccattact gcatgtagaa gcttgataaa ctatcgccac caatttattt
     3901 ttgttgcgat attgttactt tcctcagtat gcagctttga aaagaccaac cctcttatcc
     3961 tttaacaatg aacaggtttt tagaggtagc ttgatgattc ctgcacatgt gatcttggct
     4021 tcaggcttaa ttttccaggt aaagcattat gagatactct tatatctctt acatactttt
     4081 gagataatgc acaagaactt cataactata tgctttagtt tctgcatttg acactgccaa
     4141 attcattaat ctctaatatc tttgttgttg atctttggta gacatgggta ctagaaaaag
     4201 caaactacac caaggtaaaa tacttttgta caaacataaa ctcgttatca cggaacatca
     4261 atggagtgta tatctaacgg agtgtagaaa catttgatta ttgcaggaag ctatctcagg
     4321 atattatcgg tttatatgga atctcttcta cgcagagtat ctgttattcc ccttcctcta
     4381 gctttcaatt tcatggtgag gatatgcagt tttctttgta tatcattctt cttcttcttt
     4441 gtagcttgga gtcaaaatcg gttccttcat gtacatacat caaggatatg tccttctgaa
     4501 tttttatatc ttgcaataaa aatgcttgta ccaattgaaa caccagcttt ttgagttcta
     4561 tgatcactga cttggttcta accaaaaaaa aaaaaatgtt taatttacat atctaaaagt
     4621 aggtttaggg aaacctaaac agtaaaatat ttgtatatta ttcgaatttc actcatcata
     4681 aaaacttaaa ttgcaccata aaattttgtt ttactattaa tgatgtaatt tgtgtaactt
     4741 aagataaaaa taatattccg taagttaacc ggctaaaacc acgtataaac cagggaacct
     4801 gttaaaccgg ttctttactg gataaagaaa tgaaagccca tgtagacagc tccattagag
     4861 cccaaaccct aaatttctca tctatataaa aggagtgaca ttagggtttt tgttcgtcct
     4921 cttaaagctt ctcgttttct ctgccgtctc tctcattcgc gcgacgcaaa cgatcttcag
     4981 gtgatcttct ttctccaaat cctctctcat aactctgatt tcgtacttgt gtatttgagc
     5041 tcacgctctg tttctctcac cacagcctct agatgcagcc ttctcttaaa cgtatgaaaa
     5101 ttgagccatc ttctcaaaag gtaaatttct gtgttcctta ttctctcaaa atcttcgatt
     5161 ttgttttcgt tcgatcccaa tttcgtatat gttctttggt ttagattctg ttaatcttag
     5221 atcgaagacg attttctggg tttgatcgtt agatatcatc ttaattctcg attagggttt
     5281 catagatatc atccgatttg ttcaaataat ttgagttttg tcgaataatt actcttcgat
     5341 ttgtgatttc tatctagatc tggtgttagt ttctagtttg tgcgatcgaa tttgtcgatt
     5401 aatctgagtt tttctgatta acagatggat tacaaggacc acgacgggga ttacaaggac
     5461 cacgacattg attacaagga tgatgatgac aagatggctc cgaagaagaa gaggaaggtt
     5521 ggcatccacg gggtgccagc tgctgacaag aagtactcga tcggcctcga tattgggact
     5581 aactctgttg gctgggccgt gatcaccgac gagtacaagg tgccctcaaa gaagttcaag
     5641 gtcctgggca acaccgatcg gcattccatc aagaagaatc tcattggcgc tctcctgttc
     5701 gacagcggcg agacggctga ggctacgcgg ctcaagcgca ccgcccgcag gcggtacacg
     5761 cgcaggaaga atcgcatctg ctacctgcag gagattttct ccaacgagat ggcgaaggtt
     5821 gacgattctt tcttccacag gctggaggag tcattcctcg tggaggagga taagaagcac
     5881 gagcggcatc caatcttcgg caacattgtc gacgaggttg cctaccacga gaagtaccct
     5941 acgatctacc atctgcggaa gaagctcgtg gactccacag ataaggcgga cctccgcctg
     6001 atctacctcg ctctggccca catgattaag ttcaggggcc atttcctgat cgagggggat
     6061 ctcaacccgg acaatagcga tgttgacaag ctgttcatcc agctcgtgca gacgtacaac
     6121 cagctcttcg aggagaaccc cattaatgcg tcaggcgtcg acgcgaaggc tatcctgtcc
     6181 gctaggctct cgaagtctcg gcgcctcgag aacctgatcg cccagctgcc gggcgagaag
     6241 aagaacggcc tgttcgggaa tctcattgcg ctcagcctgg ggctcacgcc caacttcaag
     6301 tcgaatttcg atctcgctga ggacgccaag ctgcagctct ccaaggacac atacgacgat
     6361 gacctggata acctcctggc ccagatcggc gatcagtacg cggacctgtt cctcgctgcc
     6421 aagaatctgt cggacgccat cctcctgtct gatattctca gggtgaacac cgagattacg
     6481 aaggctccgc tctcagcctc catgatcaag cgctacgacg agcaccatca ggatctgacc
     6541 ctcctgaagg cgctggtcag gcagcagctc cccgagaagt acaaggagat cttcttcgat
     6601 cagtcgaaga acggctacgc tgggtacatt gacggcgggg cctctcagga ggagttctac
     6661 aagttcatca agccgattct ggagaagatg gacggcacgg aggagctgct ggtgaagctc
     6721 aatcgcgagg acctcctgag gaagcagcgg acattcgata acggcagcat cccacaccag
     6781 attcatctcg gggagctgca cgctatcctg aggaggcagg aggacttcta ccctttcctc
     6841 aaggataacc gcgagaagat cgagaagatt ctgactttca ggatcccgta ctacgtcggc
     6901 ccactcgcta ggggcaactc ccgcttcgct tggatgaccc gcaagtcaga ggagacgatc
     6961 acgccgtgga acttcgagga ggtggtcgac aagggcgcta gcgctcagtc gttcatcgag
     7021 aggatgacga atttcgacaa gaacctgcca aatgagaagg tgctccctaa gcactcgctc
     7081 ctgtacgagt acttcacagt ctacaacgag ctgactaagg tgaagtatgt gaccgagggc
     7141 atgaggaagc cggctttcct gtctggggag cagaagaagg ccatcgtgga cctcctgttc
     7201 aagaccaacc ggaaggtcac ggttaagcag ctcaaggagg actacttcaa gaagattgag
     7261 tgcttcgatt cggtcgagat ctctggcgtt gaggaccgct tcaacgcctc cctggggacc
     7321 taccacgatc tcctgaagat cattaaggat aaggacttcc tggacaacga ggagaatgag
     7381 gatatcctcg aggacattgt gctgacactc actctgttcg aggaccggga gatgatcgag
     7441 gagcgcctga agacttacgc ccatctcttc gatgacaagg tcatgaagca gctcaagagg
     7501 aggaggtaca ccggctgggg gaggctgagc aggaagctca tcaacggcat tcgggacaag
     7561 cagtccggga agacgatcct cgacttcctg aagagcgatg gcttcgcgaa ccgcaatttc
     7621 atgcagctga ttcacgatga cagcctcaca ttcaaggagg atatccagaa ggctcaggtg
     7681 agcggccagg gggactcgct gcacgagcat atcgcgaacc tcgctggctc gccagctatc
     7741 aagaagggga ttctgcagac cgtgaaggtt gtggacgagc tggtgaaggt catgggcagg
     7801 cacaagcctg agaacatcgt cattgagatg gcccgggaga atcagaccac gcagaagggc
     7861 cagaagaact cacgcgagag gatgaagagg atcgaggagg gcattaagga gctggggtcc
     7921 cagatcctca aggagcaccc ggtggagaac acgcagctgc agaatgagaa gctctacctg
     7981 tactacctcc agaatggccg cgatatgtat gtggaccagg agctggatat taacaggctc
     8041 agcgattacg acgtcgatca tatcgttcca cagtcattcc tgaaggatga ctccattgac
     8101 aacaaggtcc tcaccaggtc ggacaagaac cggggcaagt ctgataatgt tccttcagag
     8161 gaggtcgtta agaagatgaa gaactactgg cgccagctcc tgaatgccaa gctgatcacg
     8221 cagcggaagt tcgataacct cacaaaggct gagaggggcg ggctctctga gctggacaag
     8281 gcgggcttca tcaagaggca gctggtcgag acacggcaga tcactaagca cgttgcgcag
     8341 attctcgact cacggatgaa cactaagtac gatgagaatg acaagctgat ccgcgaggtg
     8401 aaggtcatca ccctgaagtc aaagctcgtc tccgacttca ggaaggattt ccagttctac
     8461 aaggttcggg agatcaacaa ttaccaccat gcccatgacg cgtacctgaa cgcggtggtc
     8521 ggcacagctc tgatcaagaa gtacccaaag ctcgagagcg agttcgtgta cggggactac
     8581 aaggtttacg atgtgaggaa gatgatcgcc aagtcggagc aggagattgg caaggctacc
     8641 gccaagtact tcttctactc taacattatg aatttcttca agacagagat cactctggcc
     8701 aatggcgaga tccggaagcg ccccctcatc gagacgaacg gcgagacggg ggagatcgtg
     8761 tgggacaagg gcagggattt cgcgaccgtc aggaaggttc tctccatgcc acaagtgaat
     8821 atcgtcaaga agacagaggt ccagactggc gggttctcta aggagtcaat tctgcctaag
     8881 cggaacagcg acaagctcat cgcccgcaag aaggactggg atccgaagaa gtacggcggg
     8941 ttcgacagcc ccactgtggc ctactcggtc ctggttgtgg cgaaggttga gaagggcaag
     9001 tccaagaagc tcaagagcgt gaaggagctg ctggggatca cgattatgga gcgctccagc
     9061 ttcgagaaga acccgatcga tttcctggag gcgaagggct acaaggaggt gaagaaggac
     9121 ctgatcatta agctccccaa gtactcactc ttcgagctgg agaacggcag gaagcggatg
     9181 ctggcttccg ctggcgagct gcagaagggg aacgagctgg ctctgccgtc caagtatgtg
     9241 aacttcctct acctggcctc ccactacgag aagctcaagg gcagccccga ggacaacgag
     9301 cagaagcagc tgttcgtcga gcagcacaag cattacctcg acgagatcat tgagcagatt
     9361 tccgagttct ccaagcgcgt gatcctggcc gacgcgaatc tggataaggt cctctccgcg
     9421 tacaacaagc accgcgacaa gccaatcagg gagcaggctg agaatatcat tcatctcttc
     9481 accctgacga acctcggcgc ccctgctgct ttcaagtact tcgacacaac tatcgatcgc
     9541 aagaggtaca caagcactaa ggaggtcctg gacgcgaccc tcatccacca gtcgattacc
     9601 ggcctctacg agacgcgcat cgacctgtct cagctcgggg gcgacaagcg gccagcggcg
     9661 acgaagaagg cggggcaggc gaagaagaag aagtgagctc agagctttcg ttcgtatcat
     9721 cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca caccagaatc
     9781 ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca tttgttgtgc
     9841 ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat ggaaatggat
     9901 ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat attatttgtt
     9961 ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa acattttgtt
    10021 ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg aggagtaaaa
    10081 cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt cagacctaga
    10141 aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca tttatgaact
    10201 ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt ataattatag
    10261 ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt tatgacttgc
    10321 caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat
    10381 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    10441 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    10501 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    10561 attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac tccaagaata
    10621 tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat
    10681 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag
    10741 aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag
    10801 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
    10861 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat ggtggagcac
    10921 gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt
    10981 gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc
    11041 tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc
    11101 gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc
    11161 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
    11221 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
    11281 gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg aaatcaccag
    11341 tctctctcta caaatctatc tctctcgagc tttcgcagat ccggggggca atgagatatg
    11401 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
    11461 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
    11521 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
    11581 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
    11641 gagtttagcg agagcctgac ctattgcatc tcccgccgtt cacagggtgt cacgttgcaa
    11701 gacctgcctg aaaccgaact gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg
    11761 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
    11821 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
    11881 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
    11941 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
    12001 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
    12061 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
    12121 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccacga
    12181 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
    12241 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
    12301 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
    12361 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa
    12421 tagagtagat gccgaccggg atctgtcgat cgacaagctc gagtttctcc ataataatgt
    12481 gtgagtagtt cccagataag ggaattaggg ttcctatagg gtttcgctca tgtgttgagc
    12541 atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct
    12601 aattcctaaa accaaaatcc agtactaaaa tccagatccc ccgaattaat tcggcgttaa
    12661 ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta
    12721 caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa
    12781 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
    12841 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
    12901 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
    12961 cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc gtcgatacta tgttatacgc
    13021 caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag
    13081 gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg
    13141 tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa
    13201 ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat
    13261 aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg
    13321 accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct
    13381 gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag
    13441 gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc
    13501 gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat
    13561 acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc
    13621 gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat
    13681 gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga
    13741 gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc
    13801 ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc
    13861 ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag
    13921 aaaataaaat attatatttt actggatgaa ttgttttagt acctagaatg catgaccaaa
    13981 atcccttaac gtgagttttc gttccactga gcgctgagag atcccctcat aatttcccca
    14041 aagcgtaacc atgtgtgaat aaattttgag ctagtagggt tgcagccacg agtaagtctt
    14101 cccttgttat tgtgtagcca gaatgccgca aaacttccat gcctaagcga actgttgaga
    14161 gtacgtttcg atttctgact gtgttagcct ggaagtgctt gtcccaacct tgtttctgag
    14221 catgaacgcc cgcaagccaa catgttagtt gaagcatcag ggcgattagc agcatgatat
    14281 caaaacgctc tgagctgctc gttcggctat ggcgtaggcc tagtccgtag gcaggacttt
    14341 tcaagtctcg gaaggtttct tcaatctgca ttcgcttcga atagatatta acaagttgtt
    14401 tgggtgttcg aatttcaaca ggtaagttag ttgctagaac ccatggctcc tttgccgacg
    14461 ctgagtagat tttaggtgac gggtggtgac aatgagtccg tgtcgagcgc tgattttttc
    14521 ggcctttaga gcgagattta tacaatagaa tttggcatga gattggattg cttttagtca
    14581 gcctcttata gcctaaagtc tttgagtgac tagatgacat atcatgtaag ttgctgatag
    14641 gtttccagtt ttccgctcct aggtctgcat attgtacttt tcctcttact cgacttaacc
    14701 agtaccaacc cagcttctca acggatttat accatggcac tttaaagcca gcatcactga
    14761 caatgagcgg tgtggtgtta ctcggtagaa tgctcgcaag gtcggctaga aattggtcat
    14821 gagctttctt tgaacattgc tctgaaagcg ggaacgcttt ctcataaaga gtaacagaac
    14881 gaccgtgtag tgcgactgaa gctcgcaata ccataagtcg tttttgctca cgaatatcag
    14941 accagtcaac aagtacaatg ggcatcgtat tgcccgaaca gataaagcta gcatgccaac
    15001 ggtatacagc gagtcgctct ttgtggaggt gacgattacc taacaatcgg tcgattcgtt
    15061 tgatgttatg ttttgttctc gctttggttg gcaggttacg gccaagttcg gtaagagtga
    15121 gagttttaca gtcaagtaat gcgtggcaag ccaacgttaa gctgttgagt cgttttaagt
    15181 gtaattcggg gcagaattgg taaagagagt cgtgtaaaat atcgagttcg cacatcttgt
    15241 tgtctgatta ttgatttttc gcgaaaccat ttgatcatat gacaagatgt gtatccacct
    15301 taacttaatg atttttacca aaatcattag gggattcatc agcactgagc gtcagacccc
    15361 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    15421 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    15481 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
    15541 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    15601 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    15661 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    15721 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    15781 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    15841 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    15901 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    15961 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    16021 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    16081 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    16141 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
    16201 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
    16261 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
    16321 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
    16381 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg
    16441 gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt
    16501 agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc
    16561 gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg
    16621 gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt
    16681 tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg
    16741 tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg
    16801 tacggctttg ggttcccaat gtacgtgcta tccacaggaa acagaccttt tcgacctttt
    16861 tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct
    16921 tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc
    16981 tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag
    17041 aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg
    17101 gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga
    17161 tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc
    17221 tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc
    17281 tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg
    17341 ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt
    17401 tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc
    17461 gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc
    17521 atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc
    17581 atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc
    17641 tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga
    17701 ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc
    17761 ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg
    17821 attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc
    17881 cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg
    17941 atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc
    18001 cagtgccatt gcagggccgg caggcaaccc agccgcttac gcctggccaa ccgcccgttc
    18061 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc
    18121 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc
    18181 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc
    18241 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg
    18301 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt
    18361 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt
    18421 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa
    18481 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact
    18541 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc
    18601 ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc
    18661 gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca
    18721 ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg
    18781 gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg
    18841 tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc
    18901 gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg
    18961 cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag
    19021 cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc
    19081 agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg
    19141 ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc
    19201 ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc
    19261 ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc
    19321 ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg
    19381 gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact
    19441 tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc
    19501 gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc
    19561 ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg
    19621 ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc
    19681 aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg
    19741 cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc
    19801 aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg
    19861 gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag
    19921 ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg
    19981 gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag
    20041 tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc
    20101 agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc
    20161 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt
    20221 tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
    20281 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct tag
//
