LOCUS       sequence-446487-       20106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single 
            intron-containing Cas9, RPS5A promoter, kanamycin and red 
            fluorescent seed selection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20106)
  TITLE     Takeuchi CRISPR/Cas9 Plasmids for multi-gene knockout in Arabidopsis
REFERENCE   2  (bases 1 to 20106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHEE-R-kan5A9si
FEATURES             Location/Qualifiers
     source          1..20106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             531..1238
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     944..963
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2181..2972
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        3124..3199
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             5428..5493
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5500..5520
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5545..9645
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5545..9645
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             9558..9566
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9646..9693
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(10339..10355)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10339..10355)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10352..10374)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10363..10379
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10387..10393,10394..10411,10412..10417))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10432..10453
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10644..11320
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     11257..11277
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11383..12180
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11440..11459)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12050..12069
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    12237..12411
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12489..12513
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12938..13732
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      15157..15745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15646..15665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15899..15916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15931..16071
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15997..16016)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     16157..16179
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      16415..16609
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16675..17748)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(18177..18806)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagtttc ttgaaaccaa attaacatag
       61 ggtttttatt taaataaaag tttaaccttc tttttaaaaa attgttcata gtgtcatgtc
      121 agaacaagag ctacaaatca cacatagcat gcataagcgg agctatgatg agtggtattg
      181 ttttgttcgt cacttgtcac tcttttccaa cacataatcc cgacaacaac gtaagagcat
      241 ctctctctct ccacacacac tcatgcatgc atgcattctt acacgtgatt gccatgcaaa
      301 tctcctttct cacctataaa tacaaaccaa cccttcacta cactcttcac tcaaaccaaa
      361 acaagaaaac atacacaaat agcaaaacaa gggcgaattc tcaacacaac atatacaaaa
      421 caaacgaatc tcaagcaatc aagcattcta cttctattgc agcaatttaa atcatttctt
      481 ttaaagcaaa agcaattttc tgaaaatttt caccatttac gaacgatagc atggtgagca
      541 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      601 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      661 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      721 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      781 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      841 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      901 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
      961 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1021 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1081 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1141 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1201 gccgccactc caccggcggc atggacgagc tgtacaagaa ggacgagctg taagcgccgc
     1261 ccggctgcat cgctgaaatc accagtctct ctctacaaat ctatctctct ctataataat
     1321 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     1381 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     1441 ctaattccta aaaccaaaat ccagtggtac caagcttcga cttgccttcc gcacaataca
     1501 tcatttcttc ttagcttttt ttcttcttct tcgttcatac agtttttttt tgtttatcag
     1561 cttacatttt cttgaaccgt agctttcgtt ttcttctttt taactttcca ttcggagttt
     1621 ttgtatcttg tttcatagtt tgtcccagga ttagaatgat taggcatcga accttcaaga
     1681 atttgattga ataaaacatc ttcattctta agatatgaag ataatcttca aaaggcccct
     1741 gggaatctga aagaagagaa gcaggcccat ttatatggga aagaacaata gtatttctta
     1801 tataggccca tttaagttga aaacaatctt caaaagtccc acatcgctta gataagaaaa
     1861 cgaagctgag tttatataca gctagagtcg aagtagtgat tgggagacca acccagtgga
     1921 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     1981 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2041 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2101 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2161 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     2221 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2281 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2341 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2401 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2461 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2521 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2581 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2641 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2701 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2761 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2821 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2881 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     2941 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     3001 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     3061 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccggtc
     3121 tcggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     3181 agtggcaccg agtcggtgct tttttttgca aaattttcca gatcgatttc ttcttcctct
     3241 gttcttcggc gttcaatttc tggggttttc tcttcgtttt ctgtaactga aacctaaaat
     3301 ttgacctaaa aaaaatctca aataatatga ttcagtggtt ttgtactttt cagttagttg
     3361 agttttgcag ttccgatgag ataaaccaat accatggtta tactagtctc aacttttgat
     3421 tcgctatttg cagtgcacct gtggcgttca tcacatcttt tgtgacactg tttgcactgg
     3481 tcattgctat tacaaaggac cttcctgatg ttgaaggaga tcgaaagtaa gtaactgcac
     3541 gcataaccat tttctttccg ctctttggct caatccattt gacagtcaaa gacaatgttt
     3601 aaccagctcc gtttgatata ttgtctttat gtgtttgttc aagcatgttt agttaatcat
     3661 gcctttgatt gatcttgaat aggttccaaa tatcaaccct ggcaacaaaa cttggagtga
     3721 gaaacattgc attcctcggt tctggacttc tgctagtaaa ttatgtttca gccatatcac
     3781 tagctttcta catgcctcag gtgaattcat ctatttccgt cttaactatt tcggttaatt
     3841 aaagcacgaa caccattact gcatgtagaa gcttgataaa ctatcgccac caatttattt
     3901 ttgttgcgat attgttactt tcctcagtat gcagctttga aaagaccaac cctcttatcc
     3961 tttaacaatg aacaggtttt tagaggtagc ttgatgattc ctgcacatgt gatcttggct
     4021 tcaggcttaa ttttccaggt aaagcattat gagatactct tatatctctt acatactttt
     4081 gagataatgc acaagaactt cataactata tgctttagtt tctgcatttg acactgccaa
     4141 attcattaat ctctaatatc tttgttgttg atctttggta gacatgggta ctagaaaaag
     4201 caaactacac caaggtaaaa tacttttgta caaacataaa ctcgttatca cggaacatca
     4261 atggagtgta tatctaacgg agtgtagaaa catttgatta ttgcaggaag ctatctcagg
     4321 atattatcgg tttatatgga atctcttcta cgcagagtat ctgttattcc ccttcctcta
     4381 gctttcaatt tcatggtgag gatatgcagt tttctttgta tatcattctt cttcttcttt
     4441 gtagcttgga gtcaaaatcg gttccttcat gtacatacat caaggatatg tccttctgaa
     4501 tttttatatc ttgcaataaa aatgcttgta ccaattgaaa caccagcttt ttgagttcta
     4561 tgatcactga cttggttcta accaaaaaaa aaaaaatgtt taatttacat atctaaaagt
     4621 aggtttaggg aaacctaaac agtaaaatat ttgtatatta ttcgaatttc actcatcata
     4681 aaaacttaaa ttgcaccata aaattttgtt ttactattaa tgatgtaatt tgtgtaactt
     4741 aagataaaaa taatattccg taagttaacc ggctaaaacc acgtataaac cagggaacct
     4801 gttaaaccgg ttctttactg gataaagaaa tgaaagccca tgtagacagc tccattagag
     4861 cccaaaccct aaatttctca tctatataaa aggagtgaca ttagggtttt tgttcgtcct
     4921 cttaaagctt ctcgttttct ctgccgtctc tctcattcgc gcgacgcaaa cgatcttcag
     4981 gtgatcttct ttctccaaat cctctctcat aactctgatt tcgtacttgt gtatttgagc
     5041 tcacgctctg tttctctcac cacagcctct agatgcagcc ttctcttaaa cgtatgaaaa
     5101 ttgagccatc ttctcaaaag gtaaatttct gtgttcctta ttctctcaaa atcttcgatt
     5161 ttgttttcgt tcgatcccaa tttcgtatat gttctttggt ttagattctg ttaatcttag
     5221 atcgaagacg attttctggg tttgatcgtt agatatcatc ttaattctcg attagggttt
     5281 catagatatc atccgatttg ttcaaataat ttgagttttg tcgaataatt actcttcgat
     5341 ttgtgatttc tatctagatc tggtgttagt ttctagtttg tgcgatcgaa tttgtcgatt
     5401 aatctgagtt tttctgatta acagatggat tacaaggacc acgacgggga ttacaaggac
     5461 cacgacattg attacaagga tgatgatgac aagatggctc cgaagaagaa gaggaaggtt
     5521 ggcatccacg gggtgccagc tgctgacaag aagtactcga tcggcctcga tattgggact
     5581 aactctgttg gctgggccgt gatcaccgac gagtacaagg tgccctcaaa gaagttcaag
     5641 gtcctgggca acaccgatcg gcattccatc aagaagaatc tcattggcgc tctcctgttc
     5701 gacagcggcg agacggctga ggctacgcgg ctcaagcgca ccgcccgcag gcggtacacg
     5761 cgcaggaaga atcgcatctg ctacctgcag gagattttct ccaacgagat ggcgaaggtt
     5821 gacgattctt tcttccacag gctggaggag tcattcctcg tggaggagga taagaagcac
     5881 gagcggcatc caatcttcgg caacattgtc gacgaggttg cctaccacga gaagtaccct
     5941 acgatctacc atctgcggaa gaagctcgtg gactccacag ataaggcgga cctccgcctg
     6001 atctacctcg ctctggccca catgattaag ttcaggggcc atttcctgat cgagggggat
     6061 ctcaacccgg acaatagcga tgttgacaag ctgttcatcc agctcgtgca gacgtacaac
     6121 cagctcttcg aggagaaccc cattaatgcg tcaggcgtcg acgcgaaggc tatcctgtcc
     6181 gctaggctct cgaagtctcg gcgcctcgag aacctgatcg cccagctgcc gggcgagaag
     6241 aagaacggcc tgttcgggaa tctcattgcg ctcagcctgg ggctcacgcc caacttcaag
     6301 tcgaatttcg atctcgctga ggacgccaag ctgcagctct ccaaggacac atacgacgat
     6361 gacctggata acctcctggc ccagatcggc gatcagtacg cggacctgtt cctcgctgcc
     6421 aagaatctgt cggacgccat cctcctgtct gatattctca gggtgaacac cgagattacg
     6481 aaggctccgc tctcagcctc catgatcaag cgctacgacg agcaccatca ggatctgacc
     6541 ctcctgaagg cgctggtcag gcagcagctc cccgagaagt acaaggagat cttcttcgat
     6601 cagtcgaaga acggctacgc tgggtacatt gacggcgggg cctctcagga ggagttctac
     6661 aagttcatca agccgattct ggagaagatg gacggcacgg aggagctgct ggtgaagctc
     6721 aatcgcgagg acctcctgag gaagcagcgg acattcgata acggcagcat cccacaccag
     6781 attcatctcg gggagctgca cgctatcctg aggaggcagg aggacttcta ccctttcctc
     6841 aaggataacc gcgagaagat cgagaagatt ctgactttca ggatcccgta ctacgtcggc
     6901 ccactcgcta ggggcaactc ccgcttcgct tggatgaccc gcaagtcaga ggagacgatc
     6961 acgccgtgga acttcgagga ggtggtcgac aagggcgcta gcgctcagtc gttcatcgag
     7021 aggatgacga atttcgacaa gaacctgcca aatgagaagg tgctccctaa gcactcgctc
     7081 ctgtacgagt acttcacagt ctacaacgag ctgactaagg tgaagtatgt gaccgagggc
     7141 atgaggaagc cggctttcct gtctggggag cagaagaagg ccatcgtgga cctcctgttc
     7201 aagaccaacc ggaaggtcac ggttaagcag ctcaaggagg actacttcaa gaagattgag
     7261 tgcttcgatt cggtcgagat ctctggcgtt gaggaccgct tcaacgcctc cctggggacc
     7321 taccacgatc tcctgaagat cattaaggat aaggacttcc tggacaacga ggagaatgag
     7381 gatatcctcg aggacattgt gctgacactc actctgttcg aggaccggga gatgatcgag
     7441 gagcgcctga agacttacgc ccatctcttc gatgacaagg tcatgaagca gctcaagagg
     7501 aggaggtaca ccggctgggg gaggctgagc aggaagctca tcaacggcat tcgggacaag
     7561 cagtccggga agacgatcct cgacttcctg aagagcgatg gcttcgcgaa ccgcaatttc
     7621 atgcagctga ttcacgatga cagcctcaca ttcaaggagg atatccagaa ggctcaggtg
     7681 agcggccagg gggactcgct gcacgagcat atcgcgaacc tcgctggctc gccagctatc
     7741 aagaagggga ttctgcagac cgtgaaggtt gtggacgagc tggtgaaggt catgggcagg
     7801 cacaagcctg agaacatcgt cattgagatg gcccgggaga atcagaccac gcagaagggc
     7861 cagaagaact cacgcgagag gatgaagagg atcgaggagg gcattaagga gctggggtcc
     7921 cagatcctca aggagcaccc ggtggagaac acgcagctgc agaatgagaa gctctacctg
     7981 tactacctcc agaatggccg cgatatgtat gtggaccagg agctggatat taacaggctc
     8041 agcgattacg acgtcgatca tatcgttcca cagtcattcc tgaaggatga ctccattgac
     8101 aacaaggtcc tcaccaggtc ggacaagaac cggggcaagt ctgataatgt tccttcagag
     8161 gaggtcgtta agaagatgaa gaactactgg cgccagctcc tgaatgccaa gctgatcacg
     8221 cagcggaagt tcgataacct cacaaaggct gagaggggcg ggctctctga gctggacaag
     8281 gcgggcttca tcaagaggca gctggtcgag acacggcaga tcactaagca cgttgcgcag
     8341 attctcgact cacggatgaa cactaagtac gatgagaatg acaagctgat ccgcgaggtg
     8401 aaggtcatca ccctgaagtc aaagctcgtc tccgacttca ggaaggattt ccagttctac
     8461 aaggttcggg agatcaacaa ttaccaccat gcccatgacg cgtacctgaa cgcggtggtc
     8521 ggcacagctc tgatcaagaa gtacccaaag ctcgagagcg agttcgtgta cggggactac
     8581 aaggtttacg atgtgaggaa gatgatcgcc aagtcggagc aggagattgg caaggctacc
     8641 gccaagtact tcttctactc taacattatg aatttcttca agacagagat cactctggcc
     8701 aatggcgaga tccggaagcg ccccctcatc gagacgaacg gcgagacggg ggagatcgtg
     8761 tgggacaagg gcagggattt cgcgaccgtc aggaaggttc tctccatgcc acaagtgaat
     8821 atcgtcaaga agacagaggt ccagactggc gggttctcta aggagtcaat tctgcctaag
     8881 cggaacagcg acaagctcat cgcccgcaag aaggactggg atccgaagaa gtacggcggg
     8941 ttcgacagcc ccactgtggc ctactcggtc ctggttgtgg cgaaggttga gaagggcaag
     9001 tccaagaagc tcaagagcgt gaaggagctg ctggggatca cgattatgga gcgctccagc
     9061 ttcgagaaga acccgatcga tttcctggag gcgaagggct acaaggaggt gaagaaggac
     9121 ctgatcatta agctccccaa gtactcactc ttcgagctgg agaacggcag gaagcggatg
     9181 ctggcttccg ctggcgagct gcagaagggg aacgagctgg ctctgccgtc caagtatgtg
     9241 aacttcctct acctggcctc ccactacgag aagctcaagg gcagccccga ggacaacgag
     9301 cagaagcagc tgttcgtcga gcagcacaag cattacctcg acgagatcat tgagcagatt
     9361 tccgagttct ccaagcgcgt gatcctggcc gacgcgaatc tggataaggt cctctccgcg
     9421 tacaacaagc accgcgacaa gccaatcagg gagcaggctg agaatatcat tcatctcttc
     9481 accctgacga acctcggcgc ccctgctgct ttcaagtact tcgacacaac tatcgatcgc
     9541 aagaggtaca caagcactaa ggaggtcctg gacgcgaccc tcatccacca gtcgattacc
     9601 ggcctctacg agacgcgcat cgacctgtct cagctcgggg gcgacaagcg gccagcggcg
     9661 acgaagaagg cggggcaggc gaagaagaag aagtgagctc agagctttcg ttcgtatcat
     9721 cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca caccagaatc
     9781 ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca tttgttgtgc
     9841 ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat ggaaatggat
     9901 ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat attatttgtt
     9961 ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa acattttgtt
    10021 ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg aggagtaaaa
    10081 cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt cagacctaga
    10141 aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca tttatgaact
    10201 ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt ataattatag
    10261 ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt tatgacttgc
    10321 caattgggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    10381 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    10441 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    10501 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggctagag
    10561 cagcttgcca acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
    10621 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
    10681 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
    10741 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
    10801 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
    10861 ccaaccacgt cttcaaagca agtggattga tgtgaacatg gtggagcacg acactctcgt
    10921 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
    10981 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
    11041 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
    11101 ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
    11161 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
    11221 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
    11281 tatataagga agttcatttc atttggagag gacacgctga aatcaccagt ctctctctac
    11341 aaatctatct ctctcgagct ttcgcagatc tgtcgatcga ccatggggat tgaacaagat
    11401 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
    11461 caacagacaa tcggctgctc tgatgccgcc gtgtttcggc tgtcagcgca ggggcgcccg
    11521 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactccagga cgaggcagcg
    11581 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
    11641 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
    11701 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
    11761 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
    11821 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
    11881 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
    11941 gtgacacatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
    12001 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
    12061 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
    12121 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
    12181 gcgggactct ggggttcgga tcgatcctct agctagagtc gatcgacaag ctcgagtttc
    12241 tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc
    12301 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
    12361 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat cccccgaatt
    12421 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
    12481 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
    12541 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
    12601 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
    12661 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
    12721 aacgatgaca gagcgttgct gcctgtgatc accgcggttt caaaatcggc tccgtcgata
    12781 ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt
    12841 tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta
    12901 tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc
    12961 ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc
    13021 tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag
    13081 ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga
    13141 aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct
    13201 gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc
    13261 tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc
    13321 ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa
    13381 taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc
    13441 gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca
    13501 cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga
    13561 tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat
    13621 cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa
    13681 gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga
    13741 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgctga gagatcccct
    13801 cataatttcc ccaaagcgta accatgtgtg aataaatttt gagctagtag ggttgcagcc
    13861 acgagtaagt cttcccttgt tattgtgtag ccagaatgcc gcaaaacttc catgcctaag
    13921 cgaactgttg agagtacgtt tcgatttctg actgtgttag cctggaagtg cttgtcccaa
    13981 ccttgtttct gagcatgaac gcccgcaagc caacatgtta gttgaagcat cagggcgatt
    14041 agcagcatga tatcaaaacg ctctgagctg ctcgttcggc tatggcgtag gcctagtccg
    14101 taggcaggac ttttcaagtc tcggaaggtt tcttcaatct gcattcgctt cgaatagata
    14161 ttaacaagtt gtttgggtgt tcgaatttca acaggtaagt tagttgctag aacccatggc
    14221 tcctttgccg acgctgagta gattttaggt gacgggtggt gacaatgagt ccgtgtcgag
    14281 cgctgatttt ttcggccttt agagcgagat ttatacaata gaatttggca tgagattgga
    14341 ttgcttttag tcagcctctt atagcctaaa gtctttgagt gactagatga catatcatgt
    14401 aagttgctga taggtttcca gttttccgct cctaggtctg catattgtac ttttcctctt
    14461 actcgactta accagtacca acccagcttc tcaacggatt tataccatgg cactttaaag
    14521 ccagcatcac tgacaatgag cggtgtggtg ttactcggta gaatgctcgc aaggtcggct
    14581 agaaattggt catgagcttt ctttgaacat tgctctgaaa gcgggaacgc tttctcataa
    14641 agagtaacag aacgaccgtg tagtgcgact gaagctcgca ataccataag tcgtttttgc
    14701 tcacgaatat cagaccagtc aacaagtaca atgggcatcg tattgcccga acagataaag
    14761 ctagcatgcc aacggtatac agcgagtcgc tctttgtgga ggtgacgatt acctaacaat
    14821 cggtcgattc gtttgatgtt atgttttgtt ctcgctttgg ttggcaggtt acggccaagt
    14881 tcggtaagag tgagagtttt acagtcaagt aatgcgtggc aagccaacgt taagctgttg
    14941 agtcgtttta agtgtaattc ggggcagaat tggtaaagag agtcgtgtaa aatatcgagt
    15001 tcgcacatct tgttgtctga ttattgattt ttcgcgaaac catttgatca tatgacaaga
    15061 tgtgtatcca ccttaactta atgattttta ccaaaatcat taggggattc atcagcactg
    15121 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    15181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    15241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    15301 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    15361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    15421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    15481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    15541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    15601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    15661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    15721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    15781 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    15841 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    15901 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
    15961 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
    16021 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
    16081 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
    16141 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
    16201 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
    16261 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
    16321 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
    16381 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
    16441 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
    16501 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
    16561 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaacagacc
    16621 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
    16681 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
    16741 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
    16801 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
    16861 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
    16921 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
    16981 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
    17041 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
    17101 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
    17161 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
    17221 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
    17281 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
    17341 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
    17401 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
    17461 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
    17521 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
    17581 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
    17641 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
    17701 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
    17761 ggcttggggg ttccagtgcc attgcagggc cggcaggcaa cccagccgct tacgcctggc
    17821 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
    17881 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
    17941 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
    18001 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
    18061 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
    18121 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
    18181 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
    18241 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
    18301 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
    18361 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
    18421 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
    18481 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
    18541 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
    18601 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
    18661 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
    18721 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
    18781 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
    18841 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
    18901 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
    18961 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
    19021 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
    19081 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
    19141 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
    19201 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
    19261 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
    19321 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
    19381 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
    19441 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    19501 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    19561 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    19621 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    19681 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    19741 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    19801 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    19861 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    19921 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    19981 gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg
    20041 aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc
    20101 tcttag
//
