LOCUS       sequence-446488-       19791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single 
            intron-containing Cas9, RPS5A promoter, bialaphos and red 
            fluorescent seed selection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19791)
  TITLE     Takeuchi CRISPR/Cas9 Plasmids for multi-gene knockout in Arabidopsis
REFERENCE   2  (bases 1 to 19791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHEE-R-bar5A9si
FEATURES             Location/Qualifiers
     source          1..19791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             531..1238
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     944..963
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2181..2972
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        3124..3199
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             5427..5492
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5499..5519
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5544..9644
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5544..9644
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             9557..9565
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9645..9692
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(10338..10354)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10338..10354)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10351..10373)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10362..10378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10386..10392,10393..10410,10411..10416))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10431..10452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10643..11319
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     11256..11276
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11364..11915
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    11922..12096
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12174..12198
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12623..13417
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14842..15430
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15331..15350
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15584..15601
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15616..15756
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15682..15701)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15842..15864
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      16100..16294
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16360..17433)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17862..18491)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagtttc ttgaaaccaa attaacatag
       61 ggtttttatt taaataaaag tttaaccttc tttttaaaaa attgttcata gtgtcatgtc
      121 agaacaagag ctacaaatca cacatagcat gcataagcgg agctatgatg agtggtattg
      181 ttttgttcgt cacttgtcac tcttttccaa cacataatcc cgacaacaac gtaagagcat
      241 ctctctctct ccacacacac tcatgcatgc atgcattctt acacgtgatt gccatgcaaa
      301 tctcctttct cacctataaa tacaaaccaa cccttcacta cactcttcac tcaaaccaaa
      361 acaagaaaac atacacaaat agcaaaacaa gggcgaattc tcaacacaac atatacaaaa
      421 caaacgaatc tcaagcaatc aagcattcta cttctattgc agcaatttaa atcatttctt
      481 ttaaagcaaa agcaattttc tgaaaatttt caccatttac gaacgatagc atggtgagca
      541 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      601 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      661 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      721 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      781 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      841 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      901 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
      961 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1021 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1081 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1141 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1201 gccgccactc caccggcggc atggacgagc tgtacaagaa ggacgagctg taagcgccgc
     1261 ccggctgcat cgctgaaatc accagtctct ctctacaaat ctatctctct ctataataat
     1321 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     1381 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     1441 ctaattccta aaaccaaaat ccagtggtac caagcttcga cttgccttcc gcacaataca
     1501 tcatttcttc ttagcttttt ttcttcttct tcgttcatac agtttttttt tgtttatcag
     1561 cttacatttt cttgaaccgt agctttcgtt ttcttctttt taactttcca ttcggagttt
     1621 ttgtatcttg tttcatagtt tgtcccagga ttagaatgat taggcatcga accttcaaga
     1681 atttgattga ataaaacatc ttcattctta agatatgaag ataatcttca aaaggcccct
     1741 gggaatctga aagaagagaa gcaggcccat ttatatggga aagaacaata gtatttctta
     1801 tataggccca tttaagttga aaacaatctt caaaagtccc acatcgctta gataagaaaa
     1861 cgaagctgag tttatataca gctagagtcg aagtagtgat tgggagacca acccagtgga
     1921 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     1981 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2041 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2101 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2161 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     2221 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2281 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2341 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2401 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2461 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2521 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2581 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2641 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2701 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2761 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2821 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2881 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     2941 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     3001 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     3061 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccggtc
     3121 tcggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     3181 agtggcaccg agtcggtgct tttttttgca aaattttcca gatcgatttc ttcttcctct
     3241 gttcttcggc gttcaatttc tggggttttc tcttcgtttt ctgtaactga aacctaaaat
     3301 ttgacctaaa aaaaatctca aataatatga ttcagtggtt ttgtactttt cagttagttg
     3361 agttttgcag ttccgatgag ataaaccaat accatggtta tactagtctc aacttttgat
     3421 tcgctatttg cagtgcacct gtggcgttca tcacatcttt tgtgacactg tttgcactgg
     3481 tcattgctat tacaaaggac cttcctgatg ttgaaggaga tcgaaagtaa gtaactgcac
     3541 gcataaccat tttctttccg ctctttggct caatccattt gacagtcaaa gacaatgttt
     3601 aaccagctcc gtttgatata ttgtctttat gtgtttgttc aagcatgttt agttaatcat
     3661 gcctttgatt gatcttgaat aggttccaaa tatcaaccct ggcaacaaaa cttggagtga
     3721 gaaacattgc attcctcggt tctggacttc tgctagtaaa ttatgtttca gccatatcac
     3781 tagctttcta catgcctcag gtgaattcat ctatttccgt cttaactatt tcggttaatt
     3841 aaagcacgaa caccattact gcatgtagaa gcttgataaa ctatcgccac caatttattt
     3901 ttgttgcgat attgttactt tcctcagtat gcagctttga aaagaccaac cctcttatcc
     3961 tttaacaatg aacaggtttt tagaggtagc ttgatgattc ctgcacatgt gatcttggct
     4021 tcaggcttaa ttttccaggt aaagcattat gagatactct tatatctctt acatactttt
     4081 gagataatgc acaagaactt cataactata tgctttagtt tctgcatttg acactgccaa
     4141 attcattaat ctctaatatc tttgttgttg atctttggta gacatgggta ctagaaaaag
     4201 caaactacac caaggtaaaa tacttttgta caaacataaa ctcgttatca cggaacatca
     4261 atggagtgta tatctaacgg agtgtagaaa catttgatta ttgcaggaag ctatctcagg
     4321 atattatcgg tttatatgga atctcttcta cgcagagtat ctgttattcc ccttcctcta
     4381 gctttcaatt tcatggtgag gatatgcagt tttctttgta tatcattctt cttcttcttt
     4441 gtagcttgga gtcaaaatcg gttccttcat gtacatacat caaggatatg tccttctgaa
     4501 tttttatatc ttgcaataaa aatgcttgta ccaattgaaa caccagcttt ttgagttcta
     4561 tgatcactga cttggttcta accaaaaaaa aaaaatgttt aatttacata tctaaaagta
     4621 ggtttaggga aacctaaaca gtaaaatatt tgtatattat tcgaatttca ctcatcataa
     4681 aaacttaaat tgcaccataa aattttgttt tactattaat gatgtaattt gtgtaactta
     4741 agataaaaat aatattccgt aagttaaccg gctaaaacca cgtataaacc agggaacctg
     4801 ttaaaccggt tctttactgg ataaagaaat gaaagcccat gtagacagct ccattagagc
     4861 ccaaacccta aatttctcat ctatataaaa ggagtgacat tagggttttt gttcgtcctc
     4921 ttaaagcttc tcgttttctc tgccgtctct ctcattcgcg cgacgcaaac gatcttcagg
     4981 tgatcttctt tctccaaatc ctctctcata actctgattt cgtacttgtg tatttgagct
     5041 cacgctctgt ttctctcacc acagcctcta gatgcagcct tctcttaaac gtatgaaaat
     5101 tgagccatct tctcaaaagg taaatttctg tgttccttat tctctcaaaa tcttcgattt
     5161 tgttttcgtt cgatcccaat ttcgtatatg ttctttggtt tagattctgt taatcttaga
     5221 tcgaagacga ttttctgggt ttgatcgtta gatatcatct taattctcga ttagggtttc
     5281 atagatatca tccgatttgt tcaaataatt tgagttttgt cgaataatta ctcttcgatt
     5341 tgtgatttct atctagatct ggtgttagtt tctagtttgt gcgatcgaat ttgtcgatta
     5401 atctgagttt ttctgattaa cagatggatt acaaggacca cgacggggat tacaaggacc
     5461 acgacattga ttacaaggat gatgatgaca agatggctcc gaagaagaag aggaaggttg
     5521 gcatccacgg ggtgccagct gctgacaaga agtactcgat cggcctcgat attgggacta
     5581 actctgttgg ctgggccgtg atcaccgacg agtacaaggt gccctcaaag aagttcaagg
     5641 tcctgggcaa caccgatcgg cattccatca agaagaatct cattggcgct ctcctgttcg
     5701 acagcggcga gacggctgag gctacgcggc tcaagcgcac cgcccgcagg cggtacacgc
     5761 gcaggaagaa tcgcatctgc tacctgcagg agattttctc caacgagatg gcgaaggttg
     5821 acgattcttt cttccacagg ctggaggagt cattcctcgt ggaggaggat aagaagcacg
     5881 agcggcatcc aatcttcggc aacattgtcg acgaggttgc ctaccacgag aagtacccta
     5941 cgatctacca tctgcggaag aagctcgtgg actccacaga taaggcggac ctccgcctga
     6001 tctacctcgc tctggcccac atgattaagt tcaggggcca tttcctgatc gagggggatc
     6061 tcaacccgga caatagcgat gttgacaagc tgttcatcca gctcgtgcag acgtacaacc
     6121 agctcttcga ggagaacccc attaatgcgt caggcgtcga cgcgaaggct atcctgtccg
     6181 ctaggctctc gaagtctcgg cgcctcgaga acctgatcgc ccagctgccg ggcgagaaga
     6241 agaacggcct gttcgggaat ctcattgcgc tcagcctggg gctcacgccc aacttcaagt
     6301 cgaatttcga tctcgctgag gacgccaagc tgcagctctc caaggacaca tacgacgatg
     6361 acctggataa cctcctggcc cagatcggcg atcagtacgc ggacctgttc ctcgctgcca
     6421 agaatctgtc ggacgccatc ctcctgtctg atattctcag ggtgaacacc gagattacga
     6481 aggctccgct ctcagcctcc atgatcaagc gctacgacga gcaccatcag gatctgaccc
     6541 tcctgaaggc gctggtcagg cagcagctcc ccgagaagta caaggagatc ttcttcgatc
     6601 agtcgaagaa cggctacgct gggtacattg acggcggggc ctctcaggag gagttctaca
     6661 agttcatcaa gccgattctg gagaagatgg acggcacgga ggagctgctg gtgaagctca
     6721 atcgcgagga cctcctgagg aagcagcgga cattcgataa cggcagcatc ccacaccaga
     6781 ttcatctcgg ggagctgcac gctatcctga ggaggcagga ggacttctac cctttcctca
     6841 aggataaccg cgagaagatc gagaagattc tgactttcag gatcccgtac tacgtcggcc
     6901 cactcgctag gggcaactcc cgcttcgctt ggatgacccg caagtcagag gagacgatca
     6961 cgccgtggaa cttcgaggag gtggtcgaca agggcgctag cgctcagtcg ttcatcgaga
     7021 ggatgacgaa tttcgacaag aacctgccaa atgagaaggt gctccctaag cactcgctcc
     7081 tgtacgagta cttcacagtc tacaacgagc tgactaaggt gaagtatgtg accgagggca
     7141 tgaggaagcc ggctttcctg tctggggagc agaagaaggc catcgtggac ctcctgttca
     7201 agaccaaccg gaaggtcacg gttaagcagc tcaaggagga ctacttcaag aagattgagt
     7261 gcttcgattc ggtcgagatc tctggcgttg aggaccgctt caacgcctcc ctggggacct
     7321 accacgatct cctgaagatc attaaggata aggacttcct ggacaacgag gagaatgagg
     7381 atatcctcga ggacattgtg ctgacactca ctctgttcga ggaccgggag atgatcgagg
     7441 agcgcctgaa gacttacgcc catctcttcg atgacaaggt catgaagcag ctcaagagga
     7501 ggaggtacac cggctggggg aggctgagca ggaagctcat caacggcatt cgggacaagc
     7561 agtccgggaa gacgatcctc gacttcctga agagcgatgg cttcgcgaac cgcaatttca
     7621 tgcagctgat tcacgatgac agcctcacat tcaaggagga tatccagaag gctcaggtga
     7681 gcggccaggg ggactcgctg cacgagcata tcgcgaacct cgctggctcg ccagctatca
     7741 agaaggggat tctgcagacc gtgaaggttg tggacgagct ggtgaaggtc atgggcaggc
     7801 acaagcctga gaacatcgtc attgagatgg cccgggagaa tcagaccacg cagaagggcc
     7861 agaagaactc acgcgagagg atgaagagga tcgaggaggg cattaaggag ctggggtccc
     7921 agatcctcaa ggagcacccg gtggagaaca cgcagctgca gaatgagaag ctctacctgt
     7981 actacctcca gaatggccgc gatatgtatg tggaccagga gctggatatt aacaggctca
     8041 gcgattacga cgtcgatcat atcgttccac agtcattcct gaaggatgac tccattgaca
     8101 acaaggtcct caccaggtcg gacaagaacc ggggcaagtc tgataatgtt ccttcagagg
     8161 aggtcgttaa gaagatgaag aactactggc gccagctcct gaatgccaag ctgatcacgc
     8221 agcggaagtt cgataacctc acaaaggctg agaggggcgg gctctctgag ctggacaagg
     8281 cgggcttcat caagaggcag ctggtcgaga cacggcagat cactaagcac gttgcgcaga
     8341 ttctcgactc acggatgaac actaagtacg atgagaatga caagctgatc cgcgaggtga
     8401 aggtcatcac cctgaagtca aagctcgtct ccgacttcag gaaggatttc cagttctaca
     8461 aggttcggga gatcaacaat taccaccatg cccatgacgc gtacctgaac gcggtggtcg
     8521 gcacagctct gatcaagaag tacccaaagc tcgagagcga gttcgtgtac ggggactaca
     8581 aggtttacga tgtgaggaag atgatcgcca agtcggagca ggagattggc aaggctaccg
     8641 ccaagtactt cttctactct aacattatga atttcttcaa gacagagatc actctggcca
     8701 atggcgagat ccggaagcgc cccctcatcg agacgaacgg cgagacgggg gagatcgtgt
     8761 gggacaaggg cagggatttc gcgaccgtca ggaaggttct ctccatgcca caagtgaata
     8821 tcgtcaagaa gacagaggtc cagactggcg ggttctctaa ggagtcaatt ctgcctaagc
     8881 ggaacagcga caagctcatc gcccgcaaga aggactggga tccgaagaag tacggcgggt
     8941 tcgacagccc cactgtggcc tactcggtcc tggttgtggc gaaggttgag aagggcaagt
     9001 ccaagaagct caagagcgtg aaggagctgc tggggatcac gattatggag cgctccagct
     9061 tcgagaagaa cccgatcgat ttcctggagg cgaagggcta caaggaggtg aagaaggacc
     9121 tgatcattaa gctccccaag tactcactct tcgagctgga gaacggcagg aagcggatgc
     9181 tggcttccgc tggcgagctg cagaagggga acgagctggc tctgccgtcc aagtatgtga
     9241 acttcctcta cctggcctcc cactacgaga agctcaaggg cagccccgag gacaacgagc
     9301 agaagcagct gttcgtcgag cagcacaagc attacctcga cgagatcatt gagcagattt
     9361 ccgagttctc caagcgcgtg atcctggccg acgcgaatct ggataaggtc ctctccgcgt
     9421 acaacaagca ccgcgacaag ccaatcaggg agcaggctga gaatatcatt catctcttca
     9481 ccctgacgaa cctcggcgcc cctgctgctt tcaagtactt cgacacaact atcgatcgca
     9541 agaggtacac aagcactaag gaggtcctgg acgcgaccct catccaccag tcgattaccg
     9601 gcctctacga gacgcgcatc gacctgtctc agctcggggg cgacaagcgg ccagcggcga
     9661 cgaagaaggc ggggcaggcg aagaagaaga agtgagctca gagctttcgt tcgtatcatc
     9721 ggtttcgaca acgttcgtca agttcaatgc atcagtttca ttgcgcacac accagaatcc
     9781 tactgagttt gagtattatg gcattgggaa aactgttttt cttgtaccat ttgttgtgct
     9841 tgtaatttac tgtgtttttt attcggtttt cgctatcgaa ctgtgaaatg gaaatggatg
     9901 gagaagagtt aatgaatgat atggtccttt tgttcattct caaattaata ttatttgttt
     9961 tttctcttat ttgttgtgtg ttgaatttga aattataaga gatatgcaaa cattttgttt
    10021 tgagtaaaaa tgtgtcaaat cgtggcctct aatgaccgaa gttaatatga ggagtaaaac
    10081 acttgtagtt gtaccattat gcttattcac taggcaacaa atatattttc agacctagaa
    10141 aagctgcaaa tgttactgaa tacaagtatg tcctcttgtg ttttagacat ttatgaactt
    10201 tcctttatgt aattttccag aatccttgtc agattctaat cattgcttta taattatagt
    10261 tatactcatg gatttgtagt tgagtatgaa aatatttttt aatgcatttt atgacttgcc
    10321 aattgggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    10381 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    10441 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    10501 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tggctagagc
    10561 agcttgccaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
    10621 tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
    10681 tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg
    10741 gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg
    10801 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
    10861 caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg tggagcacga cactctcgtc
    10921 tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa
    10981 caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc
    11041 aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag
    11101 gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg
    11161 agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat
    11221 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct
    11281 atataaggaa gttcatttca tttggagagg acacgctgaa atcaccagtc tctctctaca
    11341 aatctatctc tctcgagtct accatgagcc cagaacgacg cccggccgac atccgccgtg
    11401 ccaccgaggc ggacatgccg gcggtctgca ccatcgtcaa ccactacatc gagacaagca
    11461 cggtcaactt ccgtaccgag ccgcaggaac cgcaggagtg gacggacgac ctcgtccgtc
    11521 tgcgggagcg ctatccctgg ctcgtcgccg aggtggacgg cgaggtcgcc ggcatcgcct
    11581 acgcgggccc ctggaaggca cgcaacgcct acgactggac ggccgagtcg accgtgtacg
    11641 tctccccccg ccaccagcgg acgggactgg gctccacgct ctacacccac ctgctgaagt
    11701 ccctggaggc acagggcttc aagagcgtgg tcgctgtcat cgggctgccc aacgacccga
    11761 gcgtgcgcat gcacgaggcg ctcggatatg ccccccgcgg catgctgcgg gcggccggct
    11821 tcaagcacgg gaactggcat gacgtgggtt tctggcagct ggacttcagc ctgccggtac
    11881 cgccccgtcc ggtcctgccc gtcaccgaga tttgactcga gtttctccat aataatgtgt
    11941 gagtagttcc cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat
    12001 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
    12061 ttcctaaaac caaaatccag tactaaaatc cagatccccc gaattaattc ggcgttaatt
    12121 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
    12181 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
    12241 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
    12301 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
    12361 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
    12421 ttgctgcctg tgatcaccgc ggtttcaaaa tcggctccgt cgatactatg ttatacgcca
    12481 actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga
    12541 acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta
    12601 gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact
    12661 gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa
    12721 gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac
    12781 cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt
    12841 tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc
    12901 cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga
    12961 gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac
    13021 gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga
    13081 tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga
    13141 ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga
    13201 cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg
    13261 cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg
    13321 ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa
    13381 aataaaatat tatattttac tggatgaatt gttttagtac ctagaatgca tgaccaaaat
    13441 cccttaacgt gagttttcgt tccactgagc gctgagagat cccctcataa tttccccaaa
    13501 gcgtaaccat gtgtgaataa attttgagct agtagggttg cagccacgag taagtcttcc
    13561 cttgttattg tgtagccaga atgccgcaaa acttccatgc ctaagcgaac tgttgagagt
    13621 acgtttcgat ttctgactgt gttagcctgg aagtgcttgt cccaaccttg tttctgagca
    13681 tgaacgcccg caagccaaca tgttagttga agcatcaggg cgattagcag catgatatca
    13741 aaacgctctg agctgctcgt tcggctatgg cgtaggccta gtccgtaggc aggacttttc
    13801 aagtctcgga aggtttcttc aatctgcatt cgcttcgaat agatattaac aagttgtttg
    13861 ggtgttcgaa tttcaacagg taagttagtt gctagaaccc atggctcctt tgccgacgct
    13921 gagtagattt taggtgacgg gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg
    13981 cctttagagc gagatttata caatagaatt tggcatgaga ttggattgct tttagtcagc
    14041 ctcttatagc ctaaagtctt tgagtgacta gatgacatat catgtaagtt gctgataggt
    14101 ttccagtttt ccgctcctag gtctgcatat tgtacttttc ctcttactcg acttaaccag
    14161 taccaaccca gcttctcaac ggatttatac catggcactt taaagccagc atcactgaca
    14221 atgagcggtg tggtgttact cggtagaatg ctcgcaaggt cggctagaaa ttggtcatga
    14281 gctttctttg aacattgctc tgaaagcggg aacgctttct cataaagagt aacagaacga
    14341 ccgtgtagtg cgactgaagc tcgcaatacc ataagtcgtt tttgctcacg aatatcagac
    14401 cagtcaacaa gtacaatggg catcgtattg cccgaacaga taaagctagc atgccaacgg
    14461 tatacagcga gtcgctcttt gtggaggtga cgattaccta acaatcggtc gattcgtttg
    14521 atgttatgtt ttgttctcgc tttggttggc aggttacggc caagttcggt aagagtgaga
    14581 gttttacagt caagtaatgc gtggcaagcc aacgttaagc tgttgagtcg ttttaagtgt
    14641 aattcggggc agaattggta aagagagtcg tgtaaaatat cgagttcgca catcttgttg
    14701 tctgattatt gatttttcgc gaaaccattt gatcatatga caagatgtgt atccacctta
    14761 acttaatgat ttttaccaaa atcattaggg gattcatcag cactgagcgt cagaccccgt
    14821 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    14881 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    14941 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
    15001 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    15061 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    15121 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    15181 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    15241 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    15301 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    15361 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    15421 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    15481 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    15541 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    15601 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
    15661 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
    15721 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
    15781 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
    15841 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt
    15901 gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag
    15961 attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga
    16021 agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc
    16081 tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt
    16141 taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg
    16201 tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta
    16261 cggctttggg ttcccaatgt acgtgctatc cacaggaaac agaccttttc gacctttttc
    16321 ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc
    16381 gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc
    16441 cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa
    16501 cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc
    16561 ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc
    16621 gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc
    16681 gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt
    16741 tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt
    16801 cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc
    16861 taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc
    16921 aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat
    16981 ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat
    17041 gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc
    17101 gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact
    17161 atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt
    17221 gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat
    17281 tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg
    17341 ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat
    17401 ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca
    17461 gtgccattgc agggccggca ggcaacccag ccgcttacgc ctggccaacc gcccgttcct
    17521 ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct
    17581 cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg
    17641 cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt
    17701 cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc
    17761 tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag
    17821 cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta
    17881 atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga
    17941 acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca
    18001 agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct
    18061 ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc
    18121 tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc
    18181 ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc
    18241 gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc
    18301 gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg
    18361 gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg
    18421 cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg
    18481 ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag
    18541 cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct
    18601 cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg
    18661 ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg
    18721 ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct
    18781 ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc
    18841 ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc
    18901 ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc
    18961 ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg
    19021 ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct
    19081 ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa
    19141 caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc
    19201 cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag
    19261 gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc
    19321 cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc
    19381 ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc
    19441 atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc
    19501 gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag
    19561 ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg
    19621 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg
    19681 atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt
    19741 tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta g
//
