LOCUS       sequence-44650-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pFRT/TO/FLAG/HA-DEST DICER
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     45..69
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             169..192
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             193..219
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    235..259
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atcagtgata gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac
       61 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
      121 cgatccagcc tccggactct agcgtttaaa cttaagcttg gtaccatgga ctacaaggac
      181 gacgatgaca agtaccctta tgacgtgccc gattacgctt tgaaccagat atcaacaagt
      241 ttgtacaaaa aagcaggctc caccatggga accaattcag tcgacatgaa aagccctgct
      301 ttgcaacccc tcagcatggc aggcctgcag ctcatgaccc ctgcttcctc accaatgggt
      361 cctttctttg gactgccatg gcaacaagaa gcaattcatg ataacattta tacgccaaga
      421 aaatatcagg ttgaactgct tgaagcagct ctggatcata ataccatcgt ctgtttaaac
      481 actggctcag ggaagacatt tattgcagta ctactcacta aagagctgtc ctatcagatc
      541 aggggagact tcagcagaaa tggaaaaagg acggtgttct tggtcaactc tgcaaaccag
      601 gttgctcaac aagtgtcagc tgtcagaact cattcagatc tcaaggttgg ggaatactca
      661 aacctagaag taaatgcatc ttggacaaaa gagagatgga accaagagtt tactaagcac
      721 caggttctca ttatgacttg ctatgtcgcc ttgaatgttt tgaaaaatgg ttacttatca
      781 ctgtcagaca ttaacctttt ggtgtttgat gagtgtcatc ttgcaatcct agaccacccc
      841 tatcgagaaa ttatgaagct ctgtgaaaat tgtccatcat gtcctcgcat tttgggacta
      901 ac
//
