LOCUS       sequence-446505-        6308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid used to generate DNA-FISH probe targeting at human L1HS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Zhang J, Ataei L, Mittal K, Wu L, Caldwell L, Huynh L, Sarajideen S,
            Tse K, Simon MM, Mazid MA, Cook DP, Trcka D, Kwan T, Hoffman MM, 
            Wrana JL, Esteban MA, Ramalho-Santos M
  TITLE     LINE1 and PRC2 control nucleolar organization and repression of the 
            8C state in human ESCs.
  JOURNAL   Dev Cell. 2024 Oct 14:S1534-5807(24)00574-4. doi: 
            10.1016/j.devcel.2024.09.024.
  PUBMED    39413784
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2024 Oct 14:S1534-5807(24)00574-4. doi: 
            10.1016/j.devcel.2024.09.024."
COMMENT     Sequence Label: pU6-hL1-5'_3.3kb plasmid
FEATURES             Location/Qualifiers
     source          1..6308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(630..1421,1422..1490))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1253..1272
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1491..1595)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1663..1681
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1719..1741)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1841..1860
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(1877..2332)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2014..2035)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2226..2245
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2407..2547
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2418..2547
                     /label=AAV2 ITR
     promoter        complement(5869..6117)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(5927..5946)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(6097..6117)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     rep_origin      complement(join(6179..6308,1..459))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6259..6278)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
       61 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      121 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      181 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      241 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      301 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      361 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      421 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      481 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      541 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      601 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      661 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      721 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg gcttccacgc
      781 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      841 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      901 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      961 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1021 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1081 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1141 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1201 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1261 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1321 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1381 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1441 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1501 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1561 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1621 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     1681 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     1741 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     1801 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     1861 ctgagagtgc accataaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt
     1921 tgttaaatca gctcattttt taaccaatag accgaaatcg gcaaaatccc ttataaatca
     1981 aaagaatagc ccgagataga gttgagtgtt gttccagttt ggaacaagag tccactatta
     2041 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     2101 cgtgaaccat cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     2161 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     2221 aaggaaggga agaaagcgaa aggagcgggc gctaaggcgc tggcaagtgt agcggtcacg
     2281 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtactatggt
     2341 tgctttgacg tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     2401 ggcgcccctg caggcagctg cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg
     2461 ggcgtcgggc gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag
     2521 tggccaactc catcactagg ggttcctgcg gccgctctag ccctggaatg tgtattaaga
     2581 ctgtaaggtg ggggggagga gccaagatgg ccgaatagga acagctccgg tctacagctc
     2641 ccagcgtgag cgacgcagaa gacggtgatt tctgcatttc catctgaggt accgggttca
     2701 tctcactagg gagtgccaga cagtgggcgc aggccagtgt gtgtgcgcac cgtgcgcgag
     2761 ccgaagcagg gcgaggcatt gcctcacctg ggaagcgcaa ggggtcaggg agttcccttt
     2821 ccgagtcaaa gaaaggggtg acggacgcac ctggaaaatc gggtcactcc cacccgaata
     2881 ttgcgctttt cagaccggct taagaaacgg cgcaccacga gactatatcc cgcacctggc
     2941 tcggagggtc ctacgcccac ggaatctcgc tgattgctag cacagcagtc tgagatcaaa
     3001 ctgcaaggcg gcaacgaggc tgggggaggg gcgcccgcca ttgcccaggc ttgcttaggt
     3061 aaacaaagca gcagggaagc tcgaactggg tggagcccac cacagctcaa ggaggcctgc
     3121 ctgcctctgt aggctccacc tctgggggca gggcacagac aaacaaaaag acagcagtaa
     3181 cctctgcaga cttaagtgtc cctgtctgac agctttgaag agagcagtgg ttctcccagc
     3241 acgcagctgg agatctgaga acgggcagac tgcctcctca agtgggtccc tgacccctga
     3301 cccccgagca gcctaactgg gaggcacccc ccagcagggg cacactgaca cctcacacgg
     3361 cagggtattc caacagacct gcagctgagg gtcctgtctg ttagaaggaa aactaacaac
     3421 cagaaaggac atctacaccg aaaacccatc tgtacatcac catcatcaaa gaccaaaagt
     3481 agataaaacc acaaagatgg ggaaaaaaca gaacagaaaa actggaaact ctaaaacgca
     3541 gagcgcctct cctcctccaa aggaacgcag ttcctcacca gcaacagaac aaagctggat
     3601 ggagaatgat tttgatgagc tgagagaaga aggcttcaga cgatcaaatt actctgagct
     3661 acgggaggac attcaaacca aaggcaaaga agttgaaaac tttgaaaaaa atttagaaga
     3721 atgtataact agaataacca atacagagaa gtgcttaaag gagctgatgg agctgaaaac
     3781 caaggctcga gaactacgtg aagaatgcag aagcctcagg agccgatgcg atcaactgga
     3841 agaaagggta tcagcaatgg aagatgaaat gaatgaaatg aagcgagaag ggaagtttag
     3901 agaaaaaaga ataaaaagaa atgagcaaag cctccaagaa atatgggact atgtgaaaag
     3961 accaaatcta cgtctgattg gtgtacctga aagtgatgtg gagaatggaa ccaagttgga
     4021 aaacactctg caggatatta tccaggagaa cttccccaat ctagcaaggc aggccaacgt
     4081 tcagattcag gaaatacaga gaacgccaca aagatactcc tcgagaagag caactccaag
     4141 acacataatt gtcagattca ccaaagttga aatgaaggaa aaaatgttaa gggcagccag
     4201 agagaaaggt cgggttaccc tcaaaggaaa gcccatcaga ctaacagcgg atctctcggc
     4261 agaaacccta caagccagaa gagagtgggg gccaatattc aacattctta aagaaaagaa
     4321 ttttcaaccc agaatttcat atccagccaa actaagcttc ataagtgaag gagaaataaa
     4381 atactttata gacaagcaaa tgttgagaga ttttgtcacc accaggcctg ccctaaaaga
     4441 gctcctgaag gaagcgctaa acatggaaag gaacaaccgg taccagccgc tgcaaaatca
     4501 tgccaaaatg taaagaccat caagactagg aagaaactgc accaactaat gagcaaaatc
     4561 accagctaac atcataatga caggatcaac ttcacacata acaatattaa ctttaaatat
     4621 aaatggacta aattctgcaa ttaaaagaca cagactggca agttggataa agagtcaaga
     4681 cccatcagtg tgctgtattc aggaaaccca tctcacgtgc agagacacac ataggctcaa
     4741 aataaaagga tggaggaaga tctaccaagc caatggaaaa caaaaaaagg caggggttgc
     4801 aatcctagtc tctgataaaa cagactttaa accaacaaag atcaaaagag acaaagaagg
     4861 ccattacata atggtaaagg gatcaattca acaagaggag ctaactatcc taaatattta
     4921 tgcacccaat acaggagcac ccagattcat aaagcaagtc ctcagtgacc tacaaagaga
     4981 cttagactcc cacacattaa taatgggaga ctttaacacc ccactgtcaa cattagacag
     5041 atcaacgaga cagaaagtca acaaggatac ccaggaattg aactcagctc tgcaccaagc
     5101 agacctaata gacatctaca gaactctcca ccccaaatca acagaatata cgtttttttc
     5161 agcaccacac cacacctatt ccaaaattga ccacatagtt ggaagcaaag ctctcctcag
     5221 caaatgtaaa agaacagaaa ttataacaaa ctatctctca gaccacagtg caatcaaact
     5281 agagctcagg attaagaatc tcactcaaag ccgctcaact acatggaaac tgaacaacct
     5341 gctcctgaat gactactggg tacataacga aatgaaggca gaaataaaga tgttctttga
     5401 aaccaacgag aacaaagaca ccacatacca gaatctctgg gacgcattca aagcagtatg
     5461 tagagggaaa tttatagcac taaatgccta caagagaaag caggaaagat ccaaaattga
     5521 caccctaaca tcacaattaa aagaactaga aaagcaagag caaacacatt caaaagctag
     5581 cagaaggcaa gaaataacta aaatcagagc agaactgaag gaaatagaga cacaaaaaac
     5641 ccttcaaaaa atcaatgaat ccaggagctg gttttttgaa aggatcaaca aaattgatag
     5701 accgctagca agactaataa agaaaaaaag agagaagaat caaatagaca caataaaaaa
     5761 tgataaaggg gatatcacca ccgatcccac agaaatacaa actaccatca gagaatacta
     5821 caaacacctc tacgcaaata aactagaaaa tctagaaaga ctggagtcgg tgtttcgtcc
     5881 tttccacaag atatataaag ccaagaaatc gaaatacttt caagttacgg taagcatatg
     5941 atagtccatt ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct
     6001 acgtcacgta ttttgtacta atatctttgt gtttacagtc aaattaattc caattatctc
     6061 tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctcaca
     6121 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6181 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6241 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6301 ctcctgtt
//
