LOCUS       sequence-446520-        6070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the fusion protein composed of the Wheat germ agluttinin 
            to the fluorescent protein mCherry. Labels the Golgi apparatus, ER 
            and the secretory pathway. Secreted form, labels Lysosomes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6070)
  TITLE     Actin-Endosomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV2-CAGIG-WGA-mCherry
FEATURES             Location/Qualifiers
     source          1..6070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(125..229)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(255..710)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(392..413)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     604..623
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   782..911
                     /label=AAV2 ITR
     promoter        1231..1506
                     /label=chicken beta-actin promoter
     primer_bind     1803..1825
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      1915..1924
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3295..3883
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3348..3368)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3766..3777)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3926..4047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3937..3956)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3991..4010
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4209..4338
                     /label=AAV2 ITR
     rep_origin      complement(4575..5163)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4655..4674)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5334..6070,1..55,56..124))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5957..5976
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
       61 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
      121 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
      181 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
      241 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
      301 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
      361 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
      421 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
      481 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
      541 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
      601 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
      661 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
      721 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
      781 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt
      841 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      901 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt agacgcgtat
      961 agcgctcata tggaagagta ccattgacgt caataatgac gtatgttccc atagtaacgc
     1021 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     1081 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     1141 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1201 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
     1261 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
     1321 gtgcagcgat gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag
     1381 gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga
     1441 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
     1501 cgggcgggcg ccactagtag atcttggagt cgctgcgcgc tgccttcgcc ccgtgccccg
     1561 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     1621 gagcgggcgg gacggccctt ctcctccggg ctgtaattag gcttggttta atgacggctt
     1681 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg
     1741 gggagcggct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
     1801 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
     1861 cttcttcttt ttcctacagc tcggtacctc gcgagcctag ggctagctgg atccgccacc
     1921 atggaaaccg acacactgct gctgtgggtg ctgcttcttt gggtgcccgg atctacaggc
     1981 gacggccctg ttatgacagc ccaggctcaa agatgtggcg agcagggcag caacatggaa
     2041 tgccccaaca acctgtgctg cagccagtac ggctattgtg gcatgggcgg cgattactgc
     2101 ggaaagggct gtcagaatgg cgcctgctgg accagcaaga gatgtggatc tcaagccggc
     2161 ggagccacct gtcctaacaa tcactgctgt tctcagtacg gccactgcgg ctttggcgcc
     2221 gaatattgtg gcgccggatg tcaaggcgga ccttgcagag ccgatattaa gtgcggctct
     2281 cagagcggcg gcaagctgtg cccaaacaat ctgtgttgtt cccagtgggg cttctgcggc
     2341 ctgggctctg aattttgtgg cggaggttgt cagtccggcg cctgcagtac agataagccc
     2401 tgtggaaaag acgctggcgg cagagtgtgc accaacaact actgctgctc taagtggggc
     2461 agctgcggca tcggccctgg atattgcgga gctggttgtc agagcggagg ctgtgatgcc
     2521 gctagagatc ctcctgtggc cagcgccact atggtgtcta agggcgaaga ggacaacatg
     2581 gccatcatca aagaattcat gcggttcaag gtgcacatgg aaggcagcgt gaacggccac
     2641 gagttcgaga ttgaaggcga aggcgagggc agaccctacg atggaacaca gaccgccaag
     2701 ctgaaagtga ccaaaggcgg ccctctgcct tttgcctggg acattctgag ccctcagttt
     2761 atgtacggca gcaaggccta cgtgaagcac cccgccgata ttcccgacta cctgaagctg
     2821 agcttccccg agggcttcaa gtgggagaga gtgatgaact tcgaggacgg cggcgtggtc
     2881 accgtgactc aagatagctc tctgcaggac ggcgagttca tctacaaagt gaagctgcgg
     2941 ggcaccaact ttccctctga tggccccgtg atgcagaaaa agaccatggg ctgggaagcc
     3001 agcagcgaga gaatgtaccc tgaagatggc gccctgaagg gcgagatcaa gcagcggctg
     3061 aaactgaagg atggcggcca ctacgacgcc gaagtgaaaa ccacctacaa ggccaagaaa
     3121 cccgtgcagc tgcctggcgc ctacaacgtg aacatcaagc tggacatcac cagccacaac
     3181 gaggactaca ccatcgtgga acagtacgag agagccgaag gcagacacag cacaggcgga
     3241 atggacgagc tgtacaagtg atagaagctt ctcgaggata tcaagcttat cgataatcaa
     3301 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3361 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3421 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3481 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3541 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3601 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3661 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3721 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3781 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     3841 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgaccc
     3901 gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     3961 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     4021 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     4081 gttcaggagg agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     4141 atcgataagg atcttcctag agcatggcta cgtagataag tagcatggcg ggttaatcat
     4201 taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     4261 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     4321 gagcgagcga gcgcgcagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     4381 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4441 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4501 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4561 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4621 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4681 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4741 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4801 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4861 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4921 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4981 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     5041 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5101 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5161 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5221 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5281 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5341 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5401 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5461 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5521 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5581 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5641 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5701 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5761 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5821 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5881 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5941 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     6001 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     6061 atgtaaccca
//
