LOCUS       sequence-446555-        3696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 HCoV-NL63 NSP16_stop
FEATURES             Location/Qualifiers
     source          1..3696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1007..1106)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1123..1142)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1124..1142)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1147..1163)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1147..1163)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1594..2385
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2478..3066
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2967..2986
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3220..3237
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3396..3423)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3515..3601)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3650..3672
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3664..3681
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3665..3681
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgtctgctga atggaagtgt
      121 ggttatgcta tgccacaaat ttataagctt caacgtatgt gtttggaacc ttgtaattta
      181 tataattatg gtgctggtat taagttgcct agtggtataa tgttaaatgt tgttaaatac
      241 actcagcttt gtcaatacct aaatagcact acaatgtgcg tacctcataa tatgcgtgtt
      301 ttgcactatg gtgctggttc tgacaaaggt gtggcacctg gtacaactgt tttaaaacgt
      361 tggctaccac ccgatgcaat aatcattgat aatgatatca atgattatgt tagtgatgca
      421 gattttagca ttacaggtga ttgtgctact gtttatcttg aagataagtt tgacttactt
      481 atttctgata tgtatgatgg tagaattaaa ttttgtgatg gtgaaaatgt ctctaaagat
      541 gggtttttta cttatcttaa tggtgttatt agagaaaaat tagctattgg tggtagtgtt
      601 gccattaaga ttacagaata tagttggaat aagtatcttt atgaattaat acaaagattt
      661 gctttttgga ctttgttttg cacgtctgtt aatacatcct cttcagaagc ttttcttatt
      721 ggtattaatt atttaggtga ctttattcaa ggtcctttta tagctggtaa cactgttcat
      781 gctaattata tattttggcg taattctact attatgtctt tgtcatacaa ttcagtttta
      841 gatttaagta agtttgaatg taaacataaa gccactgttg ttgttacact taaagatagt
      901 gatgtaaatg atatggtttt gagtttgatt aagagtggta ggttgttgtt acgcaataat
      961 ggtcgttttg gtggttttag taatcattta gtctcaacta aatgataccc aactttcttg
     1021 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     1081 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     1141 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     1201 attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga cagaaatgcc
     1261 tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
     1321 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     1381 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     1441 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     1501 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     1561 cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta
     1621 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     1681 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
     1741 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     1801 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     1861 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     1921 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     1981 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     2041 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     2101 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     2161 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     2221 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     2281 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     2341 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca
     2401 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     2461 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2521 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2581 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2641 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2701 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2761 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2821 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2881 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2941 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3001 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3061 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3121 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3181 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3241 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3301 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     3361 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     3421 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     3481 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     3541 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
     3601 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     3661 cgttgtaaaa cgacggccag tcttaagctc gggccc
//
