LOCUS       sequence-446556-        9083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR 
            insertion, Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X2 Puro DEST (w16-1)
FEATURES             Location/Qualifiers
     source          1..9083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..589
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(54..74)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(472..483)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        603..1102
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(638..661)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1035..1054
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1123..1722
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1123..1142)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1619..1639
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    1903..2027
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2068..2373)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2170..2189)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(2715..3374)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3289..3308
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(3428..3434,3435..3452,3453..3458))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(3483..3607)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     LTR             3682..3862
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3934..4055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3971..3990)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4025..4044
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4095..4230
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4250..4269)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4251..4269)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4279..4296)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4279..4295)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4288..4310)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4437..4892
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4524..4543)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4734..4755
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4918..5022
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5023..5091,5092..5883)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5241..5260)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6054..6642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6543..6562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6796..6813
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6930..6951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6966..6971,6972..6989,6990..6996)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7004..7020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7009..7031
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7028..7044
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7028..7044
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7063..7083
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7065..7083
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7111..7337
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7338..7518
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7565..7690
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8183..8416
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8601..8645
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8912..9028
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
       61 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
      121 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
      181 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
      241 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
      301 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
      361 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
      421 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
      481 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
      541 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcta
      601 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
      661 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
      721 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
      781 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
      841 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
      901 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
      961 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
     1021 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
     1081 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc
     1141 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     1201 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     1261 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     1321 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     1381 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     1441 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     1501 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     1561 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     1621 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     1681 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     1741 cagcgcccga ccgaaaggag cgcacgaccc catgcatctc gagggcccgg tacctttaag
     1801 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     1861 ggaagggcta gactcgagcg gccgccactg tgctggatat caaccacttt gtacaagaaa
     1921 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
     1981 aaacagacta cataatactg taaaacacaa catatccagt cactatggtc gacctgcaga
     2041 ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt taatggcgtt
     2101 tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa cggagaccgg
     2161 cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg
     2221 gtaaagttca cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcaccat
     2281 ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct
     2341 ctctctttta taggtgtaaa ccttaaactg catttcacca gcccctgttc tcgtcagcaa
     2401 aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt
     2461 tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg
     2521 agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt
     2581 aatacgctgc ttcatagcat acctcttttt gacatacttc gggtatacat atcagtatat
     2641 attcttatac cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc ttttagtaag
     2701 ccggatccag atctttacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     2761 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     2821 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     2881 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     2941 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     3001 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     3061 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     3121 catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg
     3181 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     3241 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     3301 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     3361 atttttttct ccattttagc ttccttagct cctgaaaatc tcgacggatc ctaactcaaa
     3421 atccacacat tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgcggcc
     3481 gccatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     3541 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct tttttgtaca
     3601 aacttgttga tatctgcaga attccaccac actggactag ctcactccca acgaagacaa
     3661 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     3721 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     3781 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     3841 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     3901 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     3961 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4021 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     4081 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4141 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     4201 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     4261 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     4321 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     4381 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     4441 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4501 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4561 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4621 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4681 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4741 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4801 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4861 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     4921 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4981 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5041 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5101 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5161 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5221 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5281 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5341 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5401 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5461 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5521 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     5581 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5641 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5701 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5761 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5821 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5881 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5941 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6001 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6061 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6121 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6181 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6241 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6301 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6361 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6421 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6481 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6541 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6601 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6661 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6721 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6781 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     6841 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     6901 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     6961 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     7021 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     7081 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     7141 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     7201 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     7261 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     7321 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7381 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7441 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7501 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     7561 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     7621 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     7681 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     7741 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     7801 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     7861 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     7921 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     7981 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     8041 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     8101 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     8161 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     8221 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     8281 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     8341 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     8401 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     8461 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     8521 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     8581 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     8641 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     8701 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     8761 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     8821 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     8881 tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt
     8941 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     9001 aaaaacaaat tacaaaattc aaaattttat cgtccagtgt ggtggaattc tgcagatatc
     9061 cagcacagtg gcggccgctc gac
//
