LOCUS       sequence-44657-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in 
            combination with pGFPns-reporter (Addgene plasmid #35626)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Naughton BJ, Han DD, Gu HH
  TITLE     Fluorescence-based evaluation of shRNA efficacy.
  JOURNAL   Anal Biochem. 2011 Oct 1;417(1):162-4. Epub 2011 Jun 13.
  PUBMED    21726522
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2011-10-1"; volume: "417(1)"; pages: "162-4. Epub 
            2011 Jun 13"
COMMENT     Sequence Label: pAAV-Ptet-RFP-shR-rtTA
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(71..197)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 tcagtcaaca agggaaagac gtcaacaaaa gcagacccgg tggccggccg cattagtctt
       61 ccaattgaaa aaagtgattt aatttatacc attttaattc agctttgtaa aaatgtatca
      121 aagagatagc aaggtattca gttttagtaa acaagataat tgctcctaaa gtagcccctt
      181 gaattccgag gcagtaggca tgaagagcgg cttgaagttg atgatgctga ccaccaccac
      241 aaacaggagg aaggcggggc tcacgaactt ccaacacagc ctccagtaca ggcctggctt
      301 aaaccccatc atctgctgga tgtcattgct gaacctgtcc acaccataaa accaggaaac
      361 tccaatagct tccataagca ccgcaaacag aatggaggtg cctgctgcaa aggtgtccag
      421 cagagtgagg acataaatcc ctccctttgt tatgcaaaac aatgccagaa ggaaggtgct
      481 gatggtgaca acacacgtga agagtttccg gtgtcgcttc aggacctgga agtcatcagc
      541 cagtccagtg atgactgctt ccatgcctcc cattgaacta tctagcccca gagccaggag
      601 catcaagaag aacaggacgg cccagaacgt ggatcctgac agagtagaga tggcttctgg
      661 atacaggata aacacaagcc cagctccttc agtggcaaca tcctcaatat tgaccttatg
      721 ttcatgggcc atataaccaa ggatggagaa gatggcaaat ccagaaataa aactggtaac
      781 acagttgatg gtgctggtca gtagagcatc cctgtaacag ttgtgcaatt atgtactgca
      841 atgcatcaat cacaccaaaa at
//
