LOCUS       sequence-446599-        8987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG epitope-tagged KaNLuc ""Red"" BRET reporter in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8987)
  AUTHORS   Labra B, Parag-Sharma K, Powers JJ, Srivastava S, Walker JR, 
            Kirkland TA, Brennan CK, Prescher JA, Amelio AL
  TITLE     Optimized in vivo multispectral bioluminescent imaging of tumor 
            biology using engineered BRET reporters.
  JOURNAL   iScience. 2024 Aug 8;27(9):110655. doi: 10.1016/j.isci.2024.110655. 
            eCollection 2024 Sep 20.
  PUBMED    39252965
REFERENCE   2  (bases 1 to 8987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2024-08-8"; volume: "27(9):110655. doi"; pages: " 
            10.1016/j.isci.2024.110655. eCollection 2024 Sep 20"
COMMENT     Sequence Label: pLenti_CMV:Flag-KaNLuc
FEATURES             Location/Qualifiers
     source          1..8987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(41..60)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(720..1511,1512..1580))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1343..1362
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1581..1685)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1711..2166)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1848..1869)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2060..2079
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2293..2315
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2307..2324
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2308..2324
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2334..2353
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2334..2352
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2373..2508
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2548..2669
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2559..2578)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2613..2632
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             2741..2974
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3061..3649
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3167..3178
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3576..3596
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3716..4315)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3799..3819)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4296..4315
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(4325..4654)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4339..4474
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4639..4659
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(4702..5217)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             complement(5929..5952)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(6011..6035)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6032..6235)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6061..6081)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6236..6539
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    6562..6678
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6945..6989)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7174..7407
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7900..8025
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8072..8252
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8253..8479)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(8507..8527)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8507..8525)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8546..8562)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8546..8562)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8559..8581)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8570..8586
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8594..8600,8601..8618,8619..8624))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8639..8660
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8777..8794)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8948..8987,1..549))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
       61 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      121 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      181 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      241 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      301 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      361 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      421 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      481 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      541 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      601 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      661 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
      721 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
      781 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
      841 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
      901 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
      961 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1021 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1081 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1141 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1201 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     1261 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     1321 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     1381 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     1441 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1501 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1561 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1621 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1681 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
     1741 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1801 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1861 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1921 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1981 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     2041 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     2101 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     2161 gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     2221 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     2281 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
     2341 actcactata gggcgaattg ggtacgtccc taggcctcca aaaaagcctc ctcactactt
     2401 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     2461 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     2521 gggcgggata gctagagcca gacatgataa gatacattga tgagtttgga caaaccacaa
     2581 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     2641 taaccattat aagctgcaat aaacaagttc ctctcactct ctgatattca tttctttgca
     2701 agttataaat actgaataat aagatgacat gaactactac tgctagagat tttccacact
     2761 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
     2821 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca
     2881 gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc aaaaagcaga
     2941 tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt taaaaagtgg
     3001 ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag gtaccgagct cgaattccag
     3061 gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga agacgcggaa
     3121 gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg agggccgaag
     3181 ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg agcagccatg
     3241 gaaaggacgt cagcttcccc gacaacacca cggaattgtc agtgcccaac agccgagccc
     3301 ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata gggaggggga
     3361 aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca gtgggggttg
     3421 cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac aactcctcat
     3481 aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata cgggaagcaa
     3541 tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga gcaacatagt
     3601 taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgattg tcgactggcg
     3661 atgcatgggg tcgtgcgctc ctttcggtcg ggcgctgcgg gtcgtggggc gggcgtcagg
     3721 caccgggctt gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg
     3781 tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga
     3841 aggcgggcac cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca
     3901 gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct
     3961 tgggccggtg cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc
     4021 tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg
     4081 gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga
     4141 gcccgacgcg cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg
     4201 gatcgacggt gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg
     4261 ccctggggac gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatggtaa
     4321 gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag
     4381 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag
     4441 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg
     4501 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     4561 ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     4621 agcctgggga ctttccacac cctaactgac acacattcca cagggtcgac cactggtcga
     4681 cgcgttaact agtccggatc cttacgccag aatgcgttcg cacagccgcc agccggtcac
     4741 tccgttgatg gttactcgga acagcaggga gccgtcgggg ttgatcaggc gctcgtcgat
     4801 aattttgttg ccgttccaca gggtccctgt tacagtgatc tttttgccgt cgaacacggc
     4861 gatgccttca tacggccgtc cgaaatagtc gatcatgttc ggcgtaaccc cgtcgattac
     4921 cagtgtgcca tagtgcagga tcaccttaaa gtgatgatca tccacagggt acaccacctt
     4981 aaaaattttt tcgatctggc ccatttggtc gccgctcaga ccttcatacg ggatgatgac
     5041 atggatgtcg atcttcagcc cattttcacc gctcaggaca atcctttgga tcggagttac
     5101 ggacaccccg agattctgaa acaaactgga cacacctccc tgttcaagga cttggtccag
     5161 gttgtagccg gctgtctgtc gccagtcccc aacgaaatct tcgagtgtga agaccatacc
     5221 tccggagata tcattaagct tgtgccccag tttgctaggg aggtcgcagt atctggccac
     5281 agccacctcg tgctgctcga cgtaggtctc tttgtcggcc tccttgattc tttccagtct
     5341 tcggtccaca tagtagacgc cgggcacctt gagattctta gcgggtttct tggatctgta
     5401 tgtgctcttc aagttgcaga tcaggtggcc cccgcccacg agcttcaggg cgtcgtcaga
     5461 tctgccttcc aggccgccgt cagcggggta cagcatctcg gtgccggcct cccagccgag
     5521 tgttttcttc tgcatcacag ggccgttgga tgtgaagttc acccctctga tcttgacgtt
     5581 gtagatgagg caaccgtcct ggaggctggt gtcctgggta gcggtcagca cgcccccgtc
     5641 ttcgtatgtg gtgactctct cccatgtgaa gccctcaggg aaggactgct taaagaagtc
     5701 ggggatgccc tgggtgtggt tgatgaaggt gtagctgccg tacatgaagc tggtagccaa
     5761 gatgtcgaag gcgaagggta gagggccgcc ctcgaccacc ttgattctca tggtctgggt
     5821 gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg aagtggtggt tgttcacggt
     5881 gccttccatg tacagcttca tgtgcatgtt ctccttaatc agctcgctct tgtcatcgtc
     5941 gtccttgtag tccattctag agtcggtgtc ttctatggag gtcaaaacag cgtggatggc
     6001 gtctccaggc gatctgacgg ttcactaaac gagctctgct tatatagacc tcccaccgta
     6061 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
     6121 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
     6181 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
     6241 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     6301 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     6361 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     6421 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     6481 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     6541 ggaactccca agcttatcga taaaattttg aattttgtaa tttgtttttg taattcttta
     6601 gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc
     6661 cccccttttc ttttaaaagt taaccgatac cgtcgagatc cgttcactaa tcgaatggat
     6721 ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc gggtcccctc
     6781 ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact
     6841 atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat
     6901 aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact tgcccattta
     6961 tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc ttcaattaag
     7021 gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca ggtcgtgtga
     7081 ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg
     7141 caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt
     7201 tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga
     7261 tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat
     7321 aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct
     7381 cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact
     7441 cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta
     7501 tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc
     7561 cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc
     7621 cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata
     7681 taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg
     7741 tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt ctagctccct
     7801 gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat
     7861 tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct
     7921 ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc
     7981 tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc
     8041 tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag
     8101 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     8161 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     8221 caggctcaga tctggtctaa ccagagagac ccgtttatgt atcgagctag gcacttaaat
     8281 acaatatctc tgcaatgcgg caattcagtg gttcgtccaa tccatgtcag acccgtctgt
     8341 tgccttccta ataaggcacg atcgtaccac cttacttcca ccaatcggca tgcacggtgc
     8401 tttttctctc cttgtaaggc atgttgctaa ctcatcgtta ccatgttgca agactacaag
     8461 agtattgcat aagactacat taagcttgca gctccagctt ttgttccctt tagtgagggt
     8521 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8581 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8641 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8701 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8761 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8821 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8881 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8941 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaat
//
