LOCUS       sequence-446604-        8737 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and
            2 (CD12), also known as hCD12, in Escherichia coli cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Hai Y, Christianson DW
  TITLE     Histone deacetylase 6 structure and molecular basis of catalysis and
            inhibition.
  JOURNAL   Nat Chem Biol. 2016 Sep;12(9):741-7. doi: 10.1038/nchembio.2134. 
            Epub 2016 Jul 25.
  PUBMED    27454933
REFERENCE   2  (bases 1 to 8737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2134"; journalName: "Nat Chem Biol"; date: 
            "2016-09"; volume: "12"; issue: "9"; pages: "741-7"
COMMENT     Sequence Label: hCD12
FEATURES             Location/Qualifiers
     source          1..8737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..96
                     /gene="lacI"
                     /label=lacI promoter
     CDS             97..1179
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(116..135)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             1988..2179
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(2173..2195)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2281..2420
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2336..2355
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2435..2452)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2606..3194)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2686..2705)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(3305..3324)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3316..4131
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3316..4131
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3389..3408)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(4224..4679)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4361..4382)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4573..4592
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(4716..4763)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     4759..4777
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(4830..4847)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(7188..7208)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(7221..8321)
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     complement(7224..7247)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             complement(8331..8348)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             8367..8389
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    8404..8428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(8428..8447)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8429..8447)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8531..8550)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga
       61 tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat
      121 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc
      181 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt
      241 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc
      301 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat
      361 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa
      421 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg
      481 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt
      541 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga
      601 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca
      661 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat
      721 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa
      781 accatgcaaa tgctgaatga gggtatcgtt cccactgcga tgctggttgc caacgatcag
      841 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc
      901 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc
      961 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct
     1021 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc
     1081 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1141 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag
     1201 ttagctcact cattaggcac cgggatctcg accgatgccc ttgagagcct tcaacccagt
     1261 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
     1321 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg
     1381 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc
     1441 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat
     1501 tatcgccggc atggcggccc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg
     1561 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac
     1621 gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg
     1681 tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg
     1741 gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa
     1801 gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg
     1861 tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag
     1921 catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga
     1981 ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca
     2041 gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg
     2101 tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga
     2161 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     2221 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     2281 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     2341 tcagagcaga ttgtactgag agtgcaccat tgcggtgtga aataccgcac agatgcgtaa
     2401 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2461 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2521 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2581 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2641 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2701 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2761 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2821 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2881 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2941 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3001 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     3061 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3121 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3181 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3241 aaaactcacg ttaagggatt ttggtcatga acaataaaac tgtctgctta cataaacagt
     3301 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctctag gccgcgatta
     3361 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     3421 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
     3481 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     3541 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     3601 ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat
     3661 tcaggtgaaa acattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     3721 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     3781 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     3841 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     3901 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     3961 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     4021 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     4081 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta agaattaatt
     4141 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4201 atttccccga aaagtgccac ctgaaattgt aaacgttact agtttgttaa aattcgcgtt
     4261 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     4321 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     4381 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     4441 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     4501 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     4561 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     4621 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     4681 ccattcgcca atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta
     4741 gaggccccaa ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc
     4801 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
     4861 gcaagcttgt cgacggcgct cgaattcgga tccgttatcc acttccaatg ttattaccgt
     4921 aagctgcgcc agtatctgcg atggacttgg atggtctcag tgattgaggc cagggcccct
     4981 gatagtgggg gccgtggcag ggtcagcagg ggtggtgggt ctccaaggag ggagcgagtg
     5041 caggcagcca tggactctga gatggatgtc aggttatagc caccctctag gataaggata
     5101 atgcggccac tggcaaggcc catcagcagg tgggtgaggt gggcataacc ctcaggtgac
     5161 acctggcagc cccccatcgg atccccccgt gcagcatcaa agccagctga gaccagcacc
     5221 agttctgggt taaactcgta ggcaatggga agcaccaggc gatgccaggc agctaggtag
     5281 tcagcatcac ccatgcgggg cccgttccat gccacgttga cggtgaagcc tgtgcccgca
     5341 gcccggccga tctggctgct ggcaccctca tcccccatgg ggaagaaggt gccatgatca
     5401 tagcggtgca gggacacata tagcacactg gggtcatcct caaacatgtg ctgagttcca
     5461 ttaccgtggt ggacatccca atccacaatc aggatccgta gggcatgccc actgatagtc
     5521 tgggcatggc gagcagccac agccacagag ttgaaaaagc aaaaaccgca agctgcatcc
     5581 tgctctgcgt ggtgtcctgg gggacgcacc acagcagcac cattcagaac ctctcctgag
     5641 agcacagcct ccaccaggcg gcaggcagcg ccagtggcaa gctgtgcaca ggcgaaggta
     5701 ctggggcaga tatagatgga gtcaaagttg gaactctcac ggtgcagctc ccgggttttc
     5761 attttctctg tggcccggag atgacccacg tactcagcac tgtgacaggt gagcagctca
     5821 gcctctgtgg cagggcgcgg tgtcagggtg aggcagcgcc cggcaaggcc cagctcctcc
     5881 agacggcaca tgatccgcaa gatgcgctgg ggtacctcag ggtggtggct gtcccacaag
     5941 ttgcagtgat tcatcatatt ttggtcatag accagccctg tgcgagactg tagcactggc
     6001 catgtcagga ttgggagcac agggggctcc cagggtcctt cctcctcgct ctcctctaca
     6061 ttgtcctcct ccatgttgtc cctctccacg gtctcagttg atctcacaag aacctcccag
     6121 aagggctcaa gggcttccag agcacaggaa actgaagcct gggcactccg gcagggggca
     6181 ccaggtgact ccagcatggg gcaagggtct cccagaaggg tgtggagcga agcactgacg
     6241 ccttcagcca gggcgcggag gttgtagcca ccctccagag acaggatcag cttgcctcct
     6301 gccagaccca tgagcaggtg ggttagctgg gcgaaccctg ccggagtggc ggccatctca
     6361 cccttggggt ccccttgcag ggcatcaaat ccagcagcca ccaggaccag ctgaggctgg
     6421 aactcgaggg cgactggcag caggacgtgc aggaaagcag caatgtagtc agcatcccgc
     6481 atccccacct ggttccaagg cacattgatg gtatatcctt ggccttggcc gaaacctgtg
     6541 gtggaccagt tagaggcctt caggtggggc cagaacctac cctgctcgta gcggtggatg
     6601 gagaaataga ggacactggg gtcctggtcg aaggtgaact gtgttccttg accgtggtgc
     6661 acatcccaat ctacgataag gaccctccgg atgcggtgtt tctgttgagc atagcgggct
     6721 gccacagcca cgtggttgaa catgcaatag ccatccataa gactgtgctg ggcgtgatgt
     6781 ccaggaggcc taatgatggc catgccattc cggatctcag cccccaggac cgcatccacc
     6841 agcctgagga cagagcctga ggccaggcag gcacaggagt atgagttcgg atgcagataa
     6901 actgagtcgt aggtgtctgc taggacacgg agttctccct cattcatgta ctgggttgtt
     6961 tccatcagat caatatattc taggctgtga accaacatca gctcttcctt ttcagcaaac
     7021 cgggcctgaa aggacacgca gcgatctagg aggccctcct ggatcagttg ctccttgatg
     7081 gcatggagcc gctcagggcc ttccgggaag ctgtcatccc agaggcaatg gaattcattt
     7141 aactgctcat ccaacaccaa gccagtgcca gcgccactac ttgcattgga ttggaagtac
     7201 aggttttcct cgatcccatt agtctgcgcg tctttcaggg cttcatcgac agtctgacga
     7261 ccgctggcgg cgttgatcac cgcagtacgc acggcatacc agaaagcgga catctgcggg
     7321 atgttcggca tgatttcacc tttctgggcg ttttccatag tggcggcaat acgtggatct
     7381 ttcgccaact cttcctcgta agacttcagc gctacggcac ccagcggttt gtctttatta
     7441 accgcttcca gaccttcatc agtcagcaga tagttttcga ggaactcttt tgccagctct
     7501 ttgttcggac tggcggcgtt aatacctgcg ctcagcacgc caacgaacgg tttggatggt
     7561 tgacccttga aggtcggcag taccgttaca ccataattca ctttgctggt gtcgatgttg
     7621 gaccatgccc acgggccgtt gatggtcatc gctgtttcgc ctttattaaa ggcagcttct
     7681 gcgatggagt aatcggtgtc tgcattcatg tgtttgtttt taatcaggtc aaccaggaag
     7741 gtcagacccg ctttcgcgcc agcgttatcc acgcccacgt ctttaatgtc gtacttgccg
     7801 ttttcatact tgaacgcata acccccgtca gcagcaatca gcggccaggt gaagtacggt
     7861 tcttgcaggt tgaacatcag cgcgctctta cctttcgctt tcagttcttt atccagcgcc
     7921 gggatctctt cccaggtttt tggcgggttc ggcagcagat ctttgttata aatcagcgat
     7981 aacgcttcaa cagcgatcgg gtaagcaatc agcttgccgt tgtaacgtac ggcatcccag
     8041 gtaaacggat acagcttgtc ctggaacgct ttgtccgggg tgatttcagc caacaggcca
     8101 gattgagcgt agccaccaaa gcggtcgtgt gcccagaaga taatgtcagg gccatcgcca
     8161 gttgccgcaa cctgtgggaa tttctcttcc agtttatccg gatgctcaac ggtgacttta
     8221 attccggtat ctttctcgaa tttcttaccg acttcagcga gaccgttata gcctttatcg
     8281 ccgttaatcc agattaccag tttaccttct tcgattttca tagaagaacc atggtgatgg
     8341 tgatggtgag aagaacccat ggtatatctc cttcttaaag ttaaacaaaa ttatttctag
     8401 aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgcgggatc
     8461 gagatctcga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     8521 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     8581 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     8641 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     8701 ctactgggct gcttcctaat gcaggagtcg cataagg
//
