LOCUS       sequence-446611-       13060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be 
            cloned in BsaI site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13060)
  AUTHORS   Karmakar S, Panda D, Panda S, Dash M, Saha R, Das P, Avinash SP, 
            Shih J, Yang Y, Nayak AK, Baig MJ, Molla KA
  TITLE     A miniature alternative to Cas9 and Cas12: Transposon-associated 
            TnpB mediates targeted genome editing in plants.
  JOURNAL   Plant Biotechnol J. 2024 Jun 28. doi: 10.1111/pbi.14416.
  PUBMED    38940895
REFERENCE   2  (bases 1 to 13060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2024 Jun 28. doi: 10.1111/pbi.14416."
COMMENT     Sequence Label: pKb-TnpB1
FEATURES             Location/Qualifiers
     source          1..13060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             26..49
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      71..323
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(332..349)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(332..348)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(341..363)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    551..575
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1876..2505
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2934..4007
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4073..4267
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4503..4525)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4611..4751
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4666..4685
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4766..4783)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4937..5525)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5017..5036)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5612..6406)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6831..6855
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6933..7107
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7148..8173)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8240..8916)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(8283..8303)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9107..9128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9143..9148,9149..9166,9167..9173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9181..9197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9186..9208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9205..9221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9205..9221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9239..9619
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     ncRNA           9868..9935
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     promoter        9961..11670
                     /gene="RUBQ2"
                     /label=Ubi promoter
                     /note="promoter for the rice (Oryza sativa) polyubiquitin 
                     gene RUBQ2"
     intron          10709..11670
                     /note="intron upstream of the coding sequence for the rice 
                     polyubiquitin gene RUBQ2"
     protein_bind    11681..11705
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      11711..11720
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11723..11743
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12992..13039
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    complement(join(13051..13060,1..15))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttgtacaaa gtggttgata acagcgacta caaggatgac gatgacaagg cttagagctc
       61 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
      121 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
      181 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
      241 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
      301 ggtgtcatct atgttactag atcgggaatt cactggccgt cgttttacaa cgtcgtgact
      361 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      421 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      481 gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac
      541 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
      601 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
      661 gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata
      721 gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt
      781 cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt
      841 ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga
      901 gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca
      961 accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg
     1021 gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg
     1081 ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg
     1141 ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca
     1201 ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc
     1261 gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga
     1321 agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg
     1381 accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc
     1441 tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg
     1501 ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc
     1561 aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga
     1621 agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc
     1681 aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc
     1741 ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga
     1801 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat
     1861 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag
     1921 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta
     1981 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg
     2041 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg
     2101 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc
     2161 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc
     2221 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg
     2281 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg
     2341 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca
     2401 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc
     2461 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag
     2521 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca
     2581 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt
     2641 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta
     2701 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg
     2761 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc
     2821 gacgccgtgg aatgccccat gtgttgagga acgggcggtt ggccaggcgt aagcggctgg
     2881 gttgcctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg
     2941 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     3001 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     3061 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     3121 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     3181 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     3241 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     3301 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     3361 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     3421 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     3481 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     3541 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     3601 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     3661 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     3721 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     3781 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     3841 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     3901 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     3961 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     4021 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa agcactcttt cctgtgaata
     4081 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     4141 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     4201 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     4261 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
     4321 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
     4381 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
     4441 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
     4501 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     4561 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     4621 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     4681 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     4741 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4801 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4861 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4921 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4981 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5041 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5101 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5161 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5221 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5281 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5341 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     5401 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5461 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5521 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5581 gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt aaaatataat
     5641 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
     5701 gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt
     5761 ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga
     5821 tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct
     5881 ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc
     5941 aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta
     6001 agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg
     6061 catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga
     6121 cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga
     6181 cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat
     6241 cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc
     6301 ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt
     6361 tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc
     6421 ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa
     6481 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt
     6541 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca
     6601 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
     6661 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
     6721 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
     6781 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
     6841 tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta
     6901 atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact ggattttggt
     6961 tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg
     7021 tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc cttatctggg
     7081 aactactcac acattattat ggagaaactc gagcttgtcg atcgacagat cccggtcggc
     7141 atctactcta tttctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
     7201 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
     7261 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
     7321 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
     7381 acgcccggag tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
     7441 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
     7501 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
     7561 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
     7621 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
     7681 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
     7741 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
     7801 ccgcagcgat cgcatccata gcctccgcga ccggttgtag aacagcgggc agttcggttt
     7861 caggcaggtc ttgcaacgtg acaccctgtg aacggcggga gatgcaatag gtcaggctct
     7921 cgctaaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
     7981 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
     8041 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
     8101 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
     8161 caggcttttt catatctcat tgccccccgg atctgcgaaa gctcgagaga gatagatttg
     8221 tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt ccttatatag
     8281 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
     8341 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
     8401 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
     8461 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
     8521 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
     8581 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
     8641 cgagagtgtc gtgctccacc atgttcacat caatccactt gctttgaaga cgtggttgga
     8701 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
     8761 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
     8821 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
     8881 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
     8941 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt ggcaagctgc
     9001 tctagccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     9061 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     9121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     9181 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg ccaagcttaa
     9241 ggaatcttta aacatacgaa cagatcactt aaagttcttc tgaagcaact taaagttatc
     9301 aggcatgcat ggatcttgga ggaatcagat gtgcagtcag ggaccatagc acaagacagg
     9361 cgtcttctac tggtgctacc agcaaatgct ggaagccggg aacactgggt acgttggaaa
     9421 ccacgtgatg tgaagaagta agataaactg taggagaaaa gcatttcgta gtgggccatg
     9481 aagcctttca ggacatgtat tgcagtatgg gccggcccat tacgcaattg gacgacaaca
     9541 aagactagta ttagtaccac ctcggctatc cacatagatc aaagctgatt taaaagagtt
     9601 gtgcagatga tccgtggcaa gattcaagaa tcccgaagtg aagaatcttg ccgtccgtac
     9661 atggacttgc ccgaactgtg gggaaaccca tgaccgagac gagaacgctg cgctgaacat
     9721 tcggcgtgaa gcgttggtgg ctgcgggaat ctcagacacc ttaaacgctc atggaggcta
     9781 tgtcagacct gcttcggcgg gcaatggtct gcgaagtgag aatcacgcga ctttagtcgt
     9841 gtgaggttca aggagaccga ggtctcgggc cggcatggtc ccagcctcct cgctggcgcc
     9901 ggctgggcaa catgcttcgg catggcgaat gggacttttt ttttgcctgc aggtccacaa
     9961 attcgggtca aggcggaagc cagcgcgcca ccccacgtca gcaaatacgg aggcgcgggg
    10021 ttgacggcgt cacccggtcc taacggcgac caacaaacca gccagaagaa attacagtaa
    10081 aaaaaaagta aattgcactt tgatccacct tttattacct aagtctcaat ttggatcacc
    10141 cttaaaccta tcttttcaat ttgggccggg ttgtggtttg gactaccatg aacaactttt
    10201 cgtcatgtct aacttccctt tcagcaaaca tatgaaccat atatagagga gatcggccgt
    10261 atactagagc tgatgtgttt aaggtcgttg attgcacgag aaaaaaaaat ccaaatcgca
    10321 acaatagcaa atttatctgg ttcaaagtga aaagatatgt ttaaaggtag tccaaagtaa
    10381 aacttataga taataaaatg tggtccaaag cgtaattcac tcaaaaaaaa tcaacgagac
    10441 gtgtaccaaa cggagacaaa cggcatcttc tcgaaatttc ccaaccgctc gctcgcccgc
    10501 ctcgtcttcc cggaaaccgc ggtggtttca gcgtggcgga ttctccaagc agacggagac
    10561 gtcacggcac gggactcctc ccaccaccca accgccataa ataccagccc cctcatctcc
    10621 tctcctcgca tcagctccac ccccgaaaaa tttctcccca atctcgcgag gctctcgtcg
    10681 tcgaatcgaa tcctctcgcg tcctcaaggt acgctgcttc tcctctcctc gcttcgtttc
    10741 gattcgattt cggacgggtg aggttgtttt gttgctagat ccgattggtg gttagggttg
    10801 tcgatgtgat tatcgtgaga tgtttagggg ttgtagatct gatggttgtg atttgggcac
    10861 ggttggttcg ataggtggaa tcgtggttag gttttgggat tggatgttgg ttctgatgat
    10921 tggggggaat ttttacggtt agatgaattg ttggatgatt cgattgggga aatcggtgta
    10981 gatctgttgg ggaattgtgg aactagtcat gcctgagtga ttggtgcgat ttgtagcgtg
    11041 ttccatcttg taggccttgt tgcgagcatg ttcagatcta ctgttccgct cttgattgag
    11101 ttattggtgc catgggttgg tgcaaacaca ggctttaata tgttatatct gttttgtgtt
    11161 tgatgtagat ctgtagggta gttcttctta gacatggttc aattatgtag cttgtgcgtt
    11221 tcgatttgat ttcatatgtt cacagattag ataatgatga actcttttaa ttaattgtca
    11281 atggtaaata ggaagtcttg tcgctatatc tgtcataatg atctcatgtt actatctgcc
    11341 agtaatttat gctaagaact atattagaat atcatgttac aatctgtagt aatatcatgt
    11401 tacaatctgt agttcatcta tataatctat tgtggtaatt tctttttact atctgtgtga
    11461 agattattgc cactagttca ttctacttat ttctgaagtt caggatacgt gtgctgttac
    11521 tacctatctg aatacatgtg tgatgtgcct gttactatct ttttgaatac atgtatgttc
    11581 tgttggaata tgtttgctgt ttgatccgtt gttgtgtcct taatcttgtg ctagttctta
    11641 ccctatctgt ttggtgatta tttcttgcag atagttatca acaagtttgt acaaaaaagc
    11701 aggcttcgaa gccaccatgg ctcctaagaa gaagcggaag gttggtattc acggggtgcc
    11761 tgcggctatg attaggaaca aggccttcgt cgtcagattg taccctaatg cagcgcagac
    11821 tgaacttatc aacagaacgt taggaagcgc gcgttttgtt tacaaccact tcctggcacg
    11881 acgcattgcc gcctataagg agagtggaaa gggacttact tatgggcaga cgtcatcaga
    11941 attaaccctc ctcaagcagg cggaggagac gtcctggctg agcgaagtag acaagttcgc
    12001 cctccaaaat tccctgaaga atctcgaaac cgcgtacaag aacttcttcc gcactgttaa
    12061 gcaatctggc aagaaagtgg gttttccgcg gttcaggaag aagcgtacgg gtgagtccta
    12121 cagaacccag tttactaaca ataacattca gatcggagag ggtcgtctaa agctacccaa
    12181 gttgggttgg gtcaaaacga aaggccaaca agatattcaa gggaagattc tcaatgtcac
    12241 agtacggcgg attcatgaag gccactacga ggccagcgtc ctttgtgagg tcgagatccc
    12301 gtatctccct gcggccccca aatttgctgc cggcgtggat gtgggcatca aggattttgc
    12361 gatagttacc gatggcgtca ggttcaagca cgagcaaaat ccaaagtact accgctccac
    12421 ccttaagcgc ttgagaaaag ctcagcagac tttgtccagg aggaaaaagg gctcggcgcg
    12481 ttatggaaaa gccaagacaa agctggcaag gatacacaaa aggatcgtga acaaacggca
    12541 ggacttcctc cacaagctaa caacatcttt ggttagagaa tatgaaataa tcgggactga
    12601 gcacctgaaa ccagacaaca tgcgcaaaaa tcgccgcctc gcgctgtcga tctctgatgc
    12661 agggtggggc gagttcattc gacagctgga gtacaaagca gcttggtacg gcaggctggt
    12721 atcgaaggtt tctccatact ttccgagttc acagttgtgc catgattgcg gcttcaaaaa
    12781 tccggaggtg aagaatcttg ccgtgcggac atggacatgt cccaactgcg gtgaaacaca
    12841 tgacagagac gagaacgctg ccctgaacat aaggcgggaa gcactcgtgg cggccggaat
    12901 cagtgacacc ctcaatgctc atgggggcta tgtgaggccg gctagcgctg ggaatggtct
    12961 tcgctcagag aatcatgcta ccttggttgt gaagcgtcct gctgccacca aaaaggccgg
    13021 acaggctaag aaaaagaagt gaatatctag acccagcttt
//
