LOCUS       sequence-446617-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Zhang Z, Rong X, Xie T, Li Z, Song H, Zhen S, Wang H, Wu J, Jaffrey 
            SR, Li X
  TITLE     Fluorogenic CRISPR for genomic DNA imaging.
  JOURNAL   Nat Commun. 2024 Jan 31;15(1):934. doi: 10.1038/s41467-024-45163-9.
  PUBMED    38296979
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-01-31"; volume: "15(1):934. doi"; pages: " 
            10.1038/s41467-024-45163-9"
COMMENT     Sequence Label: Pepper-fused sgRNA
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(361..949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(441..460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggctttat atatcttgtg gaaaggacga aacaccgggt cttcgagaag acctgtttga
       61 gagctaggcc ggctcgtctt gtggaaagga cgaaacaccg ggtcttcgag aagacctgtt
      121 tgagagctag gccggctcgt tgagctcatt agctccgagc cggcctagca agttcaaata
      181 aggctagtcc gttatcaact tggccggctc gttgagctca ttagctccga gccggccaag
      241 tggcaccgag tcggtgcttt ttttaagctt gggccgctcg aggtacctct ctacatatga
      301 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      361 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      421 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      481 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      541 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      601 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      661 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      721 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      781 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      841 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      901 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggntctcaag aagatccttt
      961 gatcttttct acggggtc
//
