LOCUS       sequence-446634-        8423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of His6-SUMO-Ub-mEos3.2-mTurqoise2-intein-CBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Williams C, Dong KC, Arkinson C, Martin A
  TITLE     The Ufd1 cofactor determines the linkage specificity of 
            polyubiquitin chain engagement by the AAA+ ATPase Cdc48.
  JOURNAL   Mol Cell. 2023 Mar 2;83(5):759-769.e7. doi: 
            10.1016/j.molcel.2023.01.016. Epub 2023 Feb 2.
  PUBMED    36736315
REFERENCE   2  (bases 1 to 8423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.01.016"; journalName: "Mol Cell"; date: 
            "2023-03-2- 2"; volume: "83"; issue: "5"; pages: "759-769.e7"
COMMENT     Sequence Label: pAM358
FEATURES             Location/Qualifiers
     source          1..8423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1918..2109
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(2103..2125)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2211..2350
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2266..2285
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2365..2382)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2536..3124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2616..2635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3295..4086,4087..4155))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3918..3937
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4156..4260)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4328..4346
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(4373..4378,4379..4395,4396..4401)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(4576..4623)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     4619..4637
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(4672..4827)
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             complement(4858..5451)
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             complement(6172..6849)
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             complement(6874..7101)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(7417..7434)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(7444..7461)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             7480..7502
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    7517..7541
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(7541..7560)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7542..7560)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7644..7663)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        7869..7946
                     /gene="lacI"
                     /label=lacI promoter
     CDS             join(7947..8423,1..606)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(7966..7985)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac
       61 cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct
      121 ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac
      181 tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc
      241 actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag
      301 tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc
      361 tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc
      421 agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg
      481 cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc
      541 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
      601 cagtgagcgc aacgcaatta atgtaagtta gctcactcat taggcaccgg gatctcgacc
      661 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
      721 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
      781 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
      841 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
      901 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
      961 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     1021 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     1081 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcattgg
     1141 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
     1201 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
     1261 ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca
     1321 agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag
     1381 aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt
     1441 gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg
     1501 cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac
     1561 tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt
     1621 tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc
     1681 gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat
     1741 tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca
     1801 caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct
     1861 cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg
     1921 accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg
     1981 cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt
     2041 cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa
     2101 aacctctgac acatgcagct cccggagacg gccacagctt gtctgtaagc ggatgccggg
     2161 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     2221 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     2281 ttgtactgag agtgcaccat tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     2341 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2401 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2461 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2521 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2581 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2641 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2701 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2761 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2821 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2881 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2941 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     3001 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3061 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3121 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3181 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3241 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3301 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3361 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3421 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3481 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3541 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3601 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3661 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3721 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     3781 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     3841 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     3901 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     3961 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4021 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4081 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4141 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4201 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4261 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     4321 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattctcat gtttgacagc
     4381 ttatcatcga taagctttaa tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg
     4441 caccgtgtat gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc accctggatg
     4501 ctgtaggcat aggcttggtt atgccggtac tgccgggcct cttgcgggat atccggatat
     4561 agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc
     4621 tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctc attgaagctg
     4681 ccacaaggca ggaacgttgg atggttccca tcctgccaag gaggtgtggg gctgcaaaca
     4741 tttatacgtc ttgccgttat atgtgaccaa ttgtcccgca gtataagctg tgttgacctg
     4801 ccaagcggat acaccaggat ttgtcgtgag gcctgagttc agaccggtga ggccagtagc
     4861 gtggctgacg aacccgttcg tgataaacgc gtggtctgcc gtgtcgacac gaaggctata
     4921 caccggctgc acgccggcgt cggtgacact ggcgactttc gcgtagtaga accgcccgtc
     4981 ggtcagctcg tcggcgatag cttgggcgtc cgggtctcgg tggtgtgctt ccaagaaacg
     5041 caccagtcca gggacgccga ctgtgtaggt tgtgggcgca aattcgggtt tcccgcgggc
     5101 aaaacctgca cagtcgacgc tgaatgcgct gcgttgaatc accgcgtaat cgcccggctt
     5161 gatttcgtcg atcagcttcc acagcagggt cggcaccccg gcgacgtcga ccaaacacaa
     5221 caacgggtgg ttcgcggtgc ccgtcacacg cagaccttcg accgtacgca ccgtgtacac
     5281 cggatgctcg ccggagtgga acagccggtc ggcgagcacg ggattgccat gccggtcaag
     5341 gactttcagg tcgatggcgt tgtcactgtt gggccgcgca cccggcacga tgtcggcgat
     5401 gcgtaccgac tcgccctcgg gtagggcaac tagtgcatct cccgtgatgc aatacttata
     5461 caattcgtcc atgcctaacg taatccctgc cgcagtaacg aactccagta aaaccatatg
     5521 atcccgtttt tcattagggt ctttgctcaa tttggactgt gtactcaaat agtgattatc
     5581 aggcagcaaa acaggaccat ccccaatcgg cgtattctgc tgatagtgat cagcgagctg
     5641 tacgccacca tcctcgatat tgtgccgaat tttgaagttg gctttgatac catttttttg
     5701 cttgtcggcc gtaatataga cattgtccga aaagtagttg tactccagct tgtgcccaag
     5761 aatattaccg tcttccttga agtcgatgcc cttaagttcg atccgattaa ccagtgtgtc
     5821 cccctcaaat tttacctctg cgcgggtctt gtaattgccg tcatccttga agaaaatggt
     5881 gcgttcttgt acatagcctt ctggcatagc gctcttaaaa aagtcgtgct gtttcatgtg
     5941 gtctggataa cgagcaaagc attgcacgcc ccaactgagg gtagtaacca acgtaggcca
     6001 tgggacaggc aatttccctg tcgtgcaaat aaatttcagg gtcaacttac cataggtcgc
     6061 gtcaccttcc ccttcacccg atacagagaa tttatgaccg ttcacatcac cgtcgagttc
     6121 gacaagaatc ggcaccaccc ctgtaaacaa ttcttcacct tttgaaacca ttcgtctggc
     6181 attgtcaggc aatccagaat gagcaacagc atgctcatac agcttaacct tgttgtaatc
     6241 tttgtcatgg cttaaaatct caatgcagtg gtccacaaag tgggcgcctg gtaacttgac
     6301 acccttctcc ttagctttgt aagtagttct gaagtcacat cggtaatggg catttccttc
     6361 aagcaacaaa gccatctcaa tatcacccgt cagcactcca tcacgcacat acattttctc
     6421 agtggagggc tcccatttca gcgtcttctt ctgcataact ggaccattgg cgggaaagtt
     6481 ggtaccataa aatcgaactt tattatagaa agtgtcccct tccattgtta tgtcgtttct
     6541 ggcgttgcaa atgcccccgt cttcgaaagt caagcttcgt tcccacgaat accccttagg
     6601 aaacgactgc ttaaaatagt cttgtatgtt gtctggatat ttggcgaata ccctgttgcc
     6661 gtaatggaat gcagtggtca ggatatcaaa ggcaaaaggc agaggtccgc cctctttgac
     6721 ttcaagatcc atactctgtt ttccctcaaa aggcttgcct gtaccatctc cgtcgatcac
     6781 aaagtggtgc ccgtttacgt tgccttccat acggagtttg atcttcatgt ctggcttaat
     6841 cgcactcata ccagatccac taccagagcc gctgccgcca cgcagacgca gaaccaggtg
     6901 cagggtgctc tccttttgaa tgttgtaatc gctcagggta cggccgtctt ccagctgttt
     6961 acccgcgaag atcagacgtt gctgatccgg cggaatgcct tccttgtctt ggattttgct
     7021 cttaacgtta tcgatggtgt cgctgctctc cacttccagg gtaatggttt taccggtcag
     7081 ggtcttaaca aagatctgca tcacggtgct gtggccaccg gtttgctcct gataaacttc
     7141 aatcacatcc tcttcctcca tacccagctc cttcggggtg tggttgtccg cgatacgctg
     7201 gccttcaaac aggaaacgca ggctgttcat cggaacacct tgacgctggc agtagctctc
     7261 tttcagtttc ttcaggtggg tggtcatttt caccttgaag tggatttcgc tgctgtcttg
     7321 accaataact ttcagcttga tgtattcgcc ttccttcttg tcgcccaggt cttcggtgct
     7381 cggtttcgct tcttgatcgc tcatctcgag catatggctg ccgcgcggca ccaggccgct
     7441 gctgtgatga tgatgatgat ggctgctgcc catggtatat ctccttctta aagttaaaca
     7501 aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta
     7561 atttcgcggg atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg
     7621 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     7681 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     7741 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     7801 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     7861 agatcccgga caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg
     7921 aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt
     7981 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg
     8041 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg
     8101 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg
     8161 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg
     8221 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc
     8281 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg
     8341 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg
     8401 accagacacc catcaacagt att
//
