LOCUS       sequence-446657-        7409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpF and GvpU.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaFU
FEATURES             Location/Qualifiers
     source          1..7409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5667..5685)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5681..5728
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5795..5886
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6751..6790
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6821..7409
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7310..7329
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaagtagaa gagaaataac
     2701 gtgcttcaca aattagtaac cgcacccatt aaccttgtag tgaaaatcgg cgaaaaagta
     2761 caggaagaag ctgataaaca gctatatgac cttccgacga ttcagcaaaa gctcattcag
     2821 cttcaaatga tgtttgagct tggtgaaatt ccagaagaag cgtttcaaga aaaagaagat
     2881 gaattgttaa tgaggtacga aattgcgaaa cgcagagaaa ttgaacaatg ggaagagcta
     2941 acacaaaaaa gaaatgagga atcctagatg ggagaattac tgtatttata cggtttaatt
     3001 ccaacaaaag aagcagcagc catagagccg tttccatctt ataaggggtt tgacggagaa
     3061 cattcactgt acccaattgc gtttgatcag gtgacggctg tagtttctaa gctggatgct
     3121 gacacctatt cagaaaaagt gattcaagaa aaaatggagc aggatatgag ctggctgcag
     3181 gaaaaagcat ttcatcatca cgaaacggta gccgctttgt acgaagaatt tacgatcatt
     3241 ccattaaaat tttgcaccat ttataaaggt gaagaaagtc tgcaggcagc tattgagatt
     3301 aacaaagaaa agatagagaa ttcactgacg ctgcttcaag gaaatgaaga gtggaatgtg
     3361 aaaatttact gtgatgatac agagcttaaa aaaggaatca gcgaaacgaa tgaaagcgtg
     3421 aaagcgaaaa aacaagaaat tagtcactta tcaccaggaa gacagttttt tgaaaagaaa
     3481 aaaatagatc agctgattga aaaagaatta gagcttcaca aaaacaaagt gtgtgaagag
     3541 atacatgaca agctaaaaga attatcgctt tatgactctg ttaaaaagaa ttggagcaaa
     3601 gacgtaactg gcgcagctga acagatggcg tggaacagcg tgtttcttct cccgtctctg
     3661 cagattacta agttcgtaaa cgaaatagaa gagcttcagc aaaggcttga aaataaaggc
     3721 tggaagtttg aagtgacggg accatggccg ccctatcatt tctcgagctt tgcgtaaagt
     3781 gaggaattaa cattatgtct cttaaacaat ccatggagaa taaagatatt gctcttattg
     3841 atattttaga tgtcatttta gataaaggag tcgccattaa aggagactta atcatttcca
     3901 tagctggcgt cgatttagtg tatttggatt tgcgggtgct tatttcttcg gttgaaacgc
     3961 ttgtgcaagc aaaagaagga aatcacaaac caatcacttc tgaacaattt gataaacaaa
     4021 aggaggaatt aatggatgca accggtcagc caagcaaatg gacgaatcca cttggatcct
     4081 gatcaagctg aacaaggctt agcgcagctt gtgatgacag ttattgagct attgaggcaa
     4141 atcgttgaac gtcatgccat gaggcgggtg gagggtggaa cgttgacgga cgaacaaatt
     4201 gaaaacttag gaattgcact aatgaactta gaagaaaaaa tggacgagtt gaaagaggtg
     4261 ttcggtctgg atgcagaaga tttaaatatt gatcttggac cgctaggcag cctgctttaa
     4321 gcggtcagta ggaggaacag tatggcagtc gaacataata tgcagtcaag tacgattgta
     4381 gatgtgctcg aaaagatttt ggataaagga gtcgttatag cgggggacat caccgtagga
     4441 attgcagatg tcgagctatt aacgataaag atccgcttga ttgtggcttc ggttgataag
     4501 gcaaaagaaa tcggcatgga ctggtgggaa aatgatccgt atctcagttc aaaaggagcc
     4561 aataacaaag cgctcgaaga agaaaataaa atgctgcatg agcggttaaa aacgcttgaa
     4621 gaaaaaatag aaacgaaacg ttaaaaactg tacgctactt aaaaaatgga gggatttaca
     4681 atggcaactg aaacaaaatt agataacaca caggcagaaa acaaggaaaa taaaaatgcg
     4741 gaaaacggtt caaaagaaaa gaacggttca aaagcaagca aaacaacaag cagcgggcca
     4801 atcaaacgag cggtagcagg aggcatcatc ggtgcaacga ttggatatgt atcgactcct
     4861 gaaaatcgaa aaagtctcct tgaccgcatt gatacagacg aattaaaaag caaagcatct
     4921 gatttaggaa caaaggtaaa agaaaaatca aaaagcagcg tggccagcct gaaaacatct
     4981 gcgggaagct tgtttaaaaa agataaagat aaatcaaaag atgatgaaga aaacgtaaat
     5041 tcttctagta gcgaaacaga agacgataac gttcaagagt acgacgagtt aaaagaagaa
     5101 aatcaaactc ttcaagatcg cttatcacag cttgaagaaa aaatgaacat gcttgttgag
     5161 cttagcctca ataaaaatca agacgaagaa gcggaagata cagattccga cgaagaagag
     5221 aacgatgaga acgatgaaaa cgatgaaaac gagcaggacg atgaaaacga agaagaaaca
     5281 tctaagccac gtaaaaagga taaaaaagaa gctgaggaag aagaaagtga aagtgacgaa
     5341 gacagcgagg aagaagagga agattctcgc tcaaacaaaa aaaataaaaa agtaaaaaca
     5401 gaagaagaag acgaagatga aagcgaagaa gaaaaaaagg aagcgaaacc aaaaaagtca
     5461 acagctaaaa aatcaaaaaa tacaaaagca aagaaaaaca cggacgaaga agatgatgaa
     5521 gcaacatctc tttctagtga agacgataca acagcctaag acgtcgtcac atttgcttct
     5581 gcttgctgca gtccggattc gcgaacgcgt ctcgagagat ccggctgcta acaaagcccg
     5641 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
     5701 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg actagtagcc
     5761 cgcctaatga gcgggctttt ttttaattcc cctatttgtt tatttttcta aatacattca
     5821 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5881 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5941 cttcctgttt ttgctcaccc agaaacgctc gtgaaagtaa aagacgcaga ggaccaattg
     6001 ggggcacgag tgggatacat agaactggac ttgaatagcg gtaaaatcct tgagagtttt
     6061 cgccctgaag agcgttttcc aatgatgagc actttcaaag ttctgctatg tggagcagta
     6121 ttatcccgtg tagatgcggg gcaagagcaa ctcggacgac gaatacacta ttcgcagaat
     6181 gacttggttg aatactcccc agtgacagaa aagcacctta cggacggaat gacggtaaga
     6241 gaattatgta gtgccgccat aacgatgagt gataacactg cggcgaactt acttctgaca
     6301 accatcggtg gaccgaagga attaaccgct tttttgcaca atatgggaga ccatgtaact
     6361 cgccttgacc gttgggaacc agaactgaat gaagccatac caaacgacga gcgagacacc
     6421 acaatgcctg cggcaatggc aacaacatta cgcaaactat taactggcga actacttact
     6481 ctggcttcac ggcaacaatt aatagactgg cttgaagcgg ataaagttgc aggaccacta
     6541 ctgcgttcgg cacttcctgc tggctggttt attgctgata aatctggggc aggagagcgt
     6601 ggttcacggg gtatcattgc cgcacttgga ccagatggta agccttcccg tatcgtagtt
     6661 atctacacga cgggtagtca ggcaactatg gacgaacgaa atagacagat tgctgaaata
     6721 ggggcttcac tgattaagca ttggtaaacc gatacaatta aaggctcctt ttggagcctt
     6781 tttttttgga cggaccggta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6841 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6901 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6961 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7021 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7081 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7141 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7201 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7261 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7321 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7381 gctcgtcagg ggggcggagc ctatggaaa
//
