LOCUS       sequence-446663-        9398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rep/Cap plasmid for the production of MyoAAV 2E, a muscle-tropic AAV
            capsid in mice..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Tabebordbar M, Lagerborg KA, Stanton A, King EM, Ye S, Tellez L, 
            Krunnfusz A, Tavakoli S, Widrick JJ, Messemer KA, Troiano EC, 
            Moghadaszadeh B, Peacker BL, Leacock KA, Horwitz N, Beggs AH, Wagers
            AJ, Sabeti PC
  TITLE     Directed evolution of a family of AAV capsid variants enabling 
            potent muscle-directed gene delivery across species.
  JOURNAL   Cell. 2021 Sep 16;184(19):4919-4938.e22. doi: 
            10.1016/j.cell.2021.08.028. Epub 2021 Sep 9.
  PUBMED    34506722
REFERENCE   2  (bases 1 to 9398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.08.028"; journalName: "Cell"; date: 
            "2021-09-16- 16"; volume: "184"; issue: "19"; pages: "4919-4938.e22"
COMMENT     Sequence Label: kiCAP-AAV-MyoAAV2E
FEATURES             Location/Qualifiers
     source          1..9398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2758..2868
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     promoter        complement(2907..2925)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2908..2925)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2945..2962)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2951..3175
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3237..3255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3274..3292
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3311..3329
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3348..3366
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3385..3403
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3422..3440
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3459..3477
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3496..3514
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             6231..6242
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(6583..6603)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6583..6601)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6622..6638)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6622..6638)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6635..6657)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6646..6662
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6670..6676,6677..6694,6695..6700))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6715..6736
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6853..6870)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7024..7612)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7104..7123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7783..8592)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8506..8525
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(8593..8697)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8723..9178)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8860..8881)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9072..9091
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9305..9327
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9319..9336
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9320..9336
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9346..9365
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9346..9364
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcgacacgcg tggaggggtg gagtcgtgac gtgaattacg tcatagggtt agggaggtcc
       61 tgtattagag gtcacgtgag tgttttgcga cattttgcga caccatgtgg tcacgctggg
      121 tatttaagcc cgagtgagca cgcagggtct ccattttgaa gcgggaggtt tgaacgcgca
      181 gccgccatgc cggggtttta cgagattgtg attaaggtcc ccagcgacct tgacgagcat
      241 ctgcccggca tttctgacag ctttgtgaac tgggtggccg agaaggaatg ggagttgccg
      301 ccagattctg acatggatct gaatctgatt gagcaggcac ccctgaccgt ggccgagaag
      361 ctgcagcgcg actttctgac ggaatggcgc cgtgtgagta aggccccgga ggctcttttc
      421 tttgtgcaat ttgagaaggg agagagctac ttccacatgc acgtgctcgt ggaaaccacc
      481 ggggtgaaat ccatggtttt gggacgtttc ctgagtcaga ttcgcgaaaa actgattcag
      541 agaatttacc gcgggatcga gccgactttg ccaaactggt tcgcggtcac aaagaccaga
      601 aatggcgccg gaggcgggaa caaggtggtg gatgagtgct acatccccaa ttacttgctc
      661 cccaaaaccc agcctgagct ccagtgggcg tggactaata tggaacagta tttaagcgcc
      721 tgtttgaatc tcacggagcg taaacggttg gtggcgcagc atctgacgca cgtgtcgcag
      781 acgcaggagc agaacaaaga gaatcagaat cccaattctg atgcgccggt gatcagatca
      841 aaaacttcag ccaggtacat ggagctggtc gggtggctcg tggacaaggg gattacctcg
      901 gagaagcagt ggatccagga ggaccaggcc tcatacatct ccttcaatgc ggcctccaac
      961 tcgcggtccc aaatcaaggc tgccttggac aatgcgggaa agattatgag cctgactaaa
     1021 accgcccccg actacctggt gggccagcag cccgtggagg acatttccag caatcggatt
     1081 tataaaattt tggaactaaa cgggtacgat ccccaatatg cggcttccgt ctttctggga
     1141 tgggccacga aaaagttcgg caagaggaac accatctggc tgtttgggcc tgcaactacc
     1201 gggaagacca acatcgcgga ggccatagcc cacactgtgc ccttctacgg gtgcgtaaac
     1261 tggaccaatg agaactttcc cttcaacgac tgtgtcgaca agatggtgat ctggtgggag
     1321 gaggggaaga tgaccgccaa ggtcgtggag tcggccaaag ccattctcgg aggaagcaag
     1381 gtgcgcgtgg accagaaatg caagtcctcg gcccagatag acccgactcc cgtgatcgtc
     1441 acctccaaca ccaacatgtg cgccgtgatt gacgggaact caacgacctt cgaacaccag
     1501 cagccgttgc aagaccggat gttcaaattt gaactcaccc gccgtctgga tcatgacttt
     1561 gggaaggtca ccaagcagga agtcaaagac tttttccggt gggcaaagga tcacgtggtt
     1621 gaggtggagc atgaattcta cgtcaaaaag ggtggagcca agaaaagacc cgcccccagt
     1681 gacgcagata taagtgagcc caaacgggtg cgcgagtcag ttgcgcagcc atcgacgtca
     1741 gacgcggaag cttcgatcaa ctacgcggac aggtaccaaa acaaatgttc tcgtcacgtg
     1801 ggcatgaatc tgatgctgtt tccctgcaga caatgcgaga gactgaatca gaattcaaat
     1861 atctgcttca ctcacggtgt caaagactgt ttagagtgct ttcccgtgtc agaatctcaa
     1921 cccgtttctg tcgtcaaaaa ggcgtatcag aaactgtgct acattcatca catcatggga
     1981 aaggtgccag acgcttgcac tgcttgcgac ctggtcaatg tggacttgga tgactgtgtt
     2041 tctgaacaat aaatgactta aaccaggtat tgctgccgat ggttatcttc cagattggct
     2101 cgaggacaac cttagtgaac tagtgccacc atgagtcggc tggataaatc taaagtcata
     2161 aacggcgctc tggaattact caatgaagtc ggtatcgaag gcctgacgac aaggaaactc
     2221 gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc
     2281 ctgctcgatg ccctggccat cgagatgctg gacaggcatc atacccactt ctgccccctg
     2341 gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcattccg ctgtgctctc
     2401 ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac
     2461 gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca
     2521 ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag
     2581 catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg
     2641 agacaagcaa ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg
     2701 gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccggccgac
     2761 gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt
     2821 gatatgctgc ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaaatg
     2881 catgaattcg atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga
     2941 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     3001 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     3061 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     3121 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     3181 gaggcggaaa gaaccagctg gggctcgaat caagctatca agtgccacct gacgtctccc
     3241 tatcagtgat agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtacgt
     3301 ctagaacgtc tccctatcag tgatagagaa gtcgacacgt ctcgagctcc ctatcagtga
     3361 tagagaaggt acgtctagaa cgtctcccta tcagtgatag agaagtcgac acgtctcgag
     3421 ctccctatca gtgatagaga aggtacgtct agaacgtctc cctatcagtg atagagaagt
     3481 cgacacgtct cgagctccct atcagtgata gagaaggtac cccctatata agcagagaga
     3541 tctgttcaaa tttgaactga ctaagcggct cccgccagat tttggcaaga ttactaagca
     3601 ggaagtcaag gacttttttg cttgggcaaa ggtcaatcag gtgccggtga ctcacgagtt
     3661 taaagttccc agggaattgg cgggaactaa aggggcggag aaatctctaa aacgcccact
     3721 gggtgacgtc accaatacta gctataaaag tctggagaag cgggccaggc tctcatttgt
     3781 tcccgagacg cctcgcagtt cagacgtgac tgttgatccc gctcctctgc gaccgctagc
     3841 ttcgatcaac tacgcggaca ggtaccaaaa caaatgttct cgtcacgtgg gcatgaatct
     3901 gatgctgttt ccctgcagac aatgcgagag actgaatcag aattcaaata tctgcttcac
     3961 tcacggtgtc aaagactgtt tagagtgctt tcccgtgtca gaatctcaac ccgtttctgt
     4021 cgtcaaaaag gcgtatcaga aactgtgcta cattcatcac atcatgggaa aggtgccaga
     4081 cgcttgcact gcttgcgacc tggtcaatgt ggacttggat gactgtgttt ctgaacaata
     4141 aatgacttaa accaggtatg gctgccgatg gttatcttcc agattggctc gaggacaacc
     4201 ttagtgaagg aattcgcgag tggtgggctt tgaaacctgg agcccctcaa cccaaggcaa
     4261 atcaacaaca tcaagacaac gctcgaggtc ttgtgcttcc gggttacaaa taccttggac
     4321 ccggcaacgg actcgacaag ggggagccgg tcaacgcagc agacgcggcg gccctcgagc
     4381 acgacaaggc ctacgaccag cagctcaagg ccggagacaa cccgtacctc aagtacaacc
     4441 acgccgacgc cgagttccag gagcggctca aagaagatac gtcttttggg ggcaacctcg
     4501 ggcgagcagt cttccaggcc aaaaagaggc ttcttgaacc tcttggtctg gttgaggaag
     4561 cggctaagac ggctcctgga aagaagaggc ctgtagagca gtctcctcag gaaccggact
     4621 cctccgcggg tattggcaaa tcgggtgcac agcccgctaa aaagagactc aatttcggtc
     4681 agactggcga cacagagtca gtcccagacc ctcaaccaat cggagaacct cccgcagccc
     4741 cctcaggtgt gggatctctt acaatggctt caggtggtgg cgcaccagtg gcagacaata
     4801 acgaaggtgc cgatggagtg ggtagttcct cgggaaattg gcattgcgat tcccaatggc
     4861 tgggggacag agtcatcacc accagcaccc gaacctgggc cctgcccacc tacaacaatc
     4921 acctctacaa gcaaatctcc aacagcacat ctggaggatc ttcaaatgac aacgcctact
     4981 tcggctacag caccccctgg gggtattttg acttcaacag attccactgc cacttctcac
     5041 cacgtgactg gcagcgactc atcaacaaca actggggatt ccggcctaag cgactcaact
     5101 tcaagctctt caacattcag gtcaaagagg ttacggacaa caatggagtc aagaccatcg
     5161 ccaataacct taccagcacg gtccaggtct tcacggactc agactatcag ctcccgtacg
     5221 tgctcgggtc ggctcacgag ggctgcctcc cgccgttccc agcggacgtt ttcatgattc
     5281 ctcagtacgg gtatctgacg cttaatgatg gaagccaggc cgtgggtcgt tcgtcctttt
     5341 actgcctgga atatttcccg tcgcaaatgc taagaacggg taacaacttc cagttcagct
     5401 acgagtttga gaacgtacct ttccatagca gctacgctca cagccaaagc ctggaccgac
     5461 taatgaatcc actcatcgac caatacttgt actatctctc aaagactatt aacggttctg
     5521 gacagaatca acaaacgcta aaattcagtg tggccggacc cagcaacatg gctgtccagg
     5581 gaagaaacta catacctgga cccagctacc gacaacaacg tgtctcaacc actgtgactc
     5641 aaaacaacaa cagcgaattt gcttggcctg gagcttcttc ttgggctctc aatggacgta
     5701 atagcttgat gaatcctgga cctgctatgg ccagccacaa agaaggagag gaccgtttct
     5761 ttcctttgtc tggatcttta atttttggca aacaaggaac tggaagagac aacgtggatg
     5821 cggacaaagt catgataacc aacgaagaag aaattaaaac tactaacccg gtagcaacgg
     5881 agtcctatgg acaagtggcc acaaaccacc agaacatctc cagaggagac caaggttacc
     5941 aagcacaggc gcagaccggc tgggttcaaa accaaggaat acttccgggt atggtttggc
     6001 aggacagaga tgtgtacctg caaggaccca tttgggccaa aattcctcac acggacggca
     6061 actttcaccc ttctccgctg atgggagggt ttggaatgaa gcacccgcct cctcagatcc
     6121 tcatcaaaaa cacacctgta cctgcggatc ctccaacggc cttcaacaag gacaagctga
     6181 actctttcat cacccagtat tctactggcc aagtcagcgt ggagatcgag tgggagctgc
     6241 agaaggaaaa cagcaagcgc tggaacccgg agatccagta cacttccaac tattacaagt
     6301 ctaataatgt tgaatttgct gttaatactg aaggtgtata tagtgaaccc cgccccattg
     6361 gcaccagata cctgactcgt aatctgtaat tgcttgttaa tcaataaacc gtttaattcg
     6421 tttcagttga actttggtct ctgcgtattt ctttcttatc tagtttccat ggctacgtag
     6481 ataagtagca tggcgggtta atcattaact acagatatcc atcacactgg cggccgctcg
     6541 actagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat
     6601 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6661 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6721 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6781 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6841 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6901 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6961 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7021 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7081 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7141 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7201 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7261 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7321 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7381 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7441 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7501 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7561 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7621 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7681 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7741 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttagaaaa actcatcgag
     7801 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
     7861 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
     7921 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     7981 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     8041 caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
     8101 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa
     8161 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
     8221 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     8281 tgctgttttt ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     8341 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
     8401 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
     8461 cttcccatac aagcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
     8521 atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgacg tttcccgttg
     8581 aatatggctc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     8641 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     8701 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
     8761 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     8821 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     8881 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     8941 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     9001 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     9061 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     9121 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     9181 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     9241 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     9301 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     9361 tagggcgaat tgggtaccgg gccccccctc gatcgagg
//
