LOCUS       sequence-44668-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in 
            combination with pGFPns-reporter (Addgene plasmid #35626)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Naughton BJ, Han DD, Gu HH
  TITLE     Fluorescence-based evaluation of shRNA efficacy.
  JOURNAL   Anal Biochem. 2011 Oct 1;417(1):162-4. Epub 2011 Jun 13.
  PUBMED    21726522
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2011-10-1"; volume: "417(1)"; pages: "162-4. Epub 
            2011 Jun 13"
COMMENT     Sequence Label: pAAV-Ptet-RFP-shR-rtTA
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           695..788
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 tcaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa
       61 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga agatcaaggt
      121 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg
      181 cagcaaagcc ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc
      241 tgagggcttc acatgggaga gaatcaccac atacgaagac gggggcgtgc tgaccgctac
      301 ccaggacacc agcttccaga acggctgcat catctacaac gtcaagatca acggggtgaa
      361 cttcccatcc aacggccctg tgatgcagaa gaaaacacgc ggctgggagg ccaacaccga
      421 gatgctgtac cccgctgacg gcggcctgag aggccacagc cagatggccc tgaagctcgt
      481 gggcgggggc tacctgcact gctccttcaa gaccacatac agatccaaga aacccgctaa
      541 gaacctcaag atgcccggct tccacttcgt ggaccacaga ctggaaagaa tcaaggaggc
      601 cgacaaagag acctacgtcg agcagcacga gatggctgtg gccaagtact gcgacctccc
      661 tagcaaactg gggcacagat aatcgatagt ttgtttgaat gaggcttcag tactttacag
      721 aatcgttgcc tgcacatctt ggaaacactt gctgggatta cttcttcagg ttaacccaac
      781 agaaggctcg aggtatattg ctgttgacag tgagcgtgaa gagccacagt gtggaagatc
      841 tgccctttct tcaaaggagt gggctatgct gtgat
//
