LOCUS       sequence-446695-        2588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ColA BBa_B0014 tetAp SD8 MjTyrRS-[p-TpaRS-1*] T3Te-T7Te DHFR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Costello A, Peterson AA, Lanster DL, Li Z, Carver GD, Badran AH
  TITLE     Efficient genetic code expansion without host genome modifications.
  JOURNAL   Nat Biotechnol. 2024 Sep 11. doi: 10.1038/s41587-024-02385-y.
  PUBMED    39261591
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Sep 11. doi: 10.1038/s41587-024-02385-y."
COMMENT     Sequence Label: pAB228q12
FEATURES             Location/Qualifiers
     source          1..2588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(147..166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      660..687
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        789..844
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    795..813
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    825..843
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      1850..1879
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      complement(1880..1907)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 aaacgtcctt agaagatgcc aggaggatac ttagcagaga gacaataagg ccggagcgaa
       61 gccgtttttc cataggctcc gcccccctga cgaacatcac gaaatctgac gctcaaatca
      121 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg atggctccct
      181 cttgcgctct cctgttcccg tcctgcggcg tccgtgttgt ggtggaggct ttacccaaat
      241 caccacgtcc cgttccgtgt agacagttcg ctccaagctg ggctgtgtgc aagaaccccc
      301 cgttcagccc gactgctgcg ccttatccgg taactatcat cttgagtcca acccggaaag
      361 acacgacaaa acgccactgg cagcagccat tggtaactga gaattagtgg atttagatat
      421 cgagagtctt gaagtggtgg cctaacagag gctacactga aaggacagta tttggtatct
      481 gcgctccact aaagccagtt accaggttaa gcagttcccc aactgactta accttcgatc
      541 aaaccgcctc cccaggcggt tttttcgttt acagagcagg agattacgac gatcgtaaaa
      601 ggatctcaag aagatcattt acggattccc gacaccaaag aggacatccg gttcacactg
      661 gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagaatat aaaaagccag
      721 attattaatc cggctttttt attatttaga cttagggacc ctgcggccgc cattaattcc
      781 taatttttgt tgacactcta tcattgatag agttatttta ccactcccta tcagtgatag
      841 agaaaagtga aatgaataga cctgcaggtg cagtaaggag gaaaaaaaaa tggatgaatt
      901 cgaaatgatt aaacgtaaca cctctgaaat tatttctgaa gaagaattgc gtgaagtttt
      961 gaaaaaagat gaaaaatctg cgattattgg tttcgaaccg tctggtaaaa ttcatttggg
     1021 tcattacttg cagattaaaa aaatgattga tttgcagaac gcgggtttcg atattattat
     1081 tattttggcg gatttgcatg cgtacttgaa ccagaaaggt gaattggatg aaattcgtaa
     1141 aattggtgat tacaacaaaa aagttttcga agcgatgggt ttgaaagcga aatacgttta
     1201 cggttctgaa ttcatgttgg ataaagatta caccttgaac gtttaccgtt tggcgttgaa
     1261 aaccaccttg aaacgtgcgc gtcgttctat ggaattgatt gcgcgtgaag atgaaaaccc
     1321 gaaagttgcg gaagttattt acccgattat gcaggttaac ggtattcatt acgttggtgg
     1381 tgatgttgcg gttggtggta tggaacagcg taaaattcat atgttggcgc gtgaattgtt
     1441 gccgaaaaaa gttgtttgta ttcataaccc ggttttgacc ggtttggatg gtgaaggtaa
     1501 aatgtcttct tctaaaggta acttcattgc ggttgatgat tctccggaag aaattcgtgc
     1561 gaaaattaaa aaagcgaaaa aaaaagcggg tgttgttgaa ggtaacccga ttatggaaat
     1621 tgcgaaatac ttcttggaat acccgttgac cattaaacgt ccggaaaaat tcggtggtga
     1681 tttgaccgtt aactcttacg aagaattgga atctttgttc aaaaacaaag aattgcagcc
     1741 gatgtctttg aaaaacgcgg ttgcggaaga attgattaaa attttggaac cgattcgtaa
     1801 acgtttgtaa acttaattaa cggcactcct cagctgtaac caactcactg gctcaccttc
     1861 acgggtgagc ctttcttcgc gcagaaaggc ccacccgaag gtgagccagt gtgattacat
     1921 ttatgcccat ggcgtttaac ctttctgcca aatctggtaa gagtagttaa tgttagacgc
     1981 gaaatcctgg gtgaaaaccg gacggaagtt agacggaatt tccgggaagt aaacatcacc
     2041 ttccggttca atatcaatgg tagaaatatg caaggtatca acctgatcaa tcaaagattt
     2101 gtaaatttca ccaccaccag aaacaataac atgatcggta atttttttca agttggtcaa
     2161 cgcatcttta atagacggga aaatcaaaac gttttcgtta tcagaggtga aagaagaacg
     2221 ggtaacaacc gcgtatttac ggttcggcaa cgcacccata gaaccgaagg ttttacgacc
     2281 aaccaacaac cactggttgt aggtaatcgc tttgaacaac aactgttcac ctttcgcaga
     2341 ccacggaata tccggaccgt taccaataac accgttttta gaaatcgcaa ccatcaaaga
     2401 caatttcata attcttcctc agaggttaac attttattag taagcatgct cgttttgtca
     2461 cccgctgatg cttaccgtta attaatataa tcacttgtat taaatagact ttctctaaaa
     2521 atacaagaca ctctgttatt acaaatcgtg cgtgccgtct atcctgcttg caaggccaaa
     2581 taggccgt
//
