LOCUS       sequence-446706-        1838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for generating a barcode library for insulator-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Tonelli A, Cousin P, Gambetta MC
  TITLE     Protocol for detecting genomic insulators in Drosophila using 
            insulator-seq, a massively parallel reporter assay.
  JOURNAL   STAR Protoc. 2024 Oct 24;5(4):103391. doi: 
            10.1016/j.xpro.2024.103391.
  PUBMED    39453817
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2024-10-24"; volume: "5(4):103391. doi"; pages: " 
            10.1016/j.xpro.2024.103391"
COMMENT     Sequence Label: insulator_seq_barcode_vector
FEATURES             Location/Qualifiers
     source          1..1838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        273..375
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             376..1035
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(442..461)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1430..1786
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgcca ggcacatggg ttttactagt
       61 atcgattcgc gacctaggta gtcggtctcg tagggaattt cgtgagacct ctggagttta
      121 gaagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgt caccgctgag
      181 caataactat cataacccct aggtgccatt tcattacctc tttctccgca cccgacatag
      241 atctgggcca acttttggcg aaaatgagac gttgatcggc acgtaagagg gtccaacttt
      301 caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga
      361 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      421 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      481 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      541 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      601 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      661 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      721 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      781 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
      841 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      901 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      961 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     1021 cagggtgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggttgctac
     1081 gcctgaataa gtgataataa gcggatgaat ggcagaaatt cgaaagcaaa ttcgacccgg
     1141 tcgtcggttc agggcagggt cgttaaatag ccgcttatgt ctattgctgg tttaccggtt
     1201 tattgactac cggaagcagt gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc
     1261 aggctctccc cgtggaggta ataattgacg atatgatcat ttattctgcc tcccagctga
     1321 cattcatccg gggtcagcac cgtttctgcg gactggcttt ctacgtgttc cgcttccttt
     1381 agcagccctt gcgccctgag tgcttgcggc agcgtgaagc taattcccat gtcagccgtt
     1441 aagtgttcct gtgtcactca aaattgcttt gagaggctct aagggcttct cagtgcgtta
     1501 catccctggc ttgttgtcca caaccgttaa accttaaaag ctttaaaagc cttatatatt
     1561 cttttttttc ttataaaact taaaacctta gaggctattt aagttgctga tttatattaa
     1621 ttttattgtt caaacatgag agcttagtac gtgaaacatg agagcttagt acgttagcca
     1681 tgagagctta gtacgttagc catgagggtt tagttcgtta aacatgagag cttagtacgt
     1741 taaacatgag agcttagtac gtgaaacatg agagcttagt acgtactatc aacaggttga
     1801 actgctgatc aacagatcct ctacgcggcc gcggtacc
//
